DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment piwi and piwil1

DIOPT Version :9

Sequence 1:	NP_001285825.1	Gene:	piwi / 34521	FlyBaseID:	FBgn0004872	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_899181.1	Gene:	piwil1 / 368200	ZFINID:	ZDB-GENE-030813-2	Length:	858	Species:	Danio rerio

Alignment Length:	894	Identity:	300/894 - (33%)
Similarity:	491/894 - (54%)	Gaps:	94/894 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 GRGRRRPLNEDDSSTSRGSGDGPRVKVFRGSSSGDPRADPRIEASRERRALEEAPRREG---GPT 67
            ||.|.|         |||.|        ||.....|.|.|.:  |:|......:...||   |..
Zfish     3 GRARAR---------SRGRG--------RGQEPAAPGAQPPV--SQEAAKPVVSTPSEGQLVGRG 48

  Fly    68 ERKP-------------------------WGDQYDY----LNTRPAELV-----SKKGTDGVPVM 98
            .:||                         .|.:.|:    ::||  :|:     ||.|..|..:.
Zfish    49 RQKPAPGAMSEEAMLQISAGFQQVKIGERGGRRRDFHDSGVHTR--QLMEHVKESKTGVSGTAIE 111

  Fly    99 LQTNFFRLKTKPEWRIVHYHVEFEPSIENPRVRMGVLSNHANLLGSGYLFDGLQLFTTRKFEQEI 163
            |:.||.||.::|.|.:..|||:::|.:|:.|:|..:|..|...||..:.|||..||...|.....
Zfish   112 LRANFMRLLSRPMWALYQYHVDYKPPMESRRLRSALLFQHEETLGKAHTFDGAILFLPNKLRNAE 176

  Fly   164 TVLSGKSKLDIEYKISIKFVGFISCAEPRFLQVLNLILRRSMKGLNLELVGRNLFDPRAKIEIRE 228
            |||..:::...:.:|::.....:..:.|..||..|::.||.::.||::.:||:.::|.....|.:
Zfish   177 TVLCSETRNGEKVEITVTLTNELPPSSPVCLQFYNILFRRILRILNMQQIGRHYYNPDDPFNIPQ 241

  Fly   229 FKMELWPGYETSIRQHEKDILLGTEITHKVMRTETIYDIM----RRCSHNPARHQDEVRVNVLDL 289
            .::.:|||:.|:|.|:|..|:|.::::|||:|:||:.|.|    ::|...  |..:.....::.|
Zfish   242 HRLTIWPGFMTTILQYESSIMLCSDVSHKVLRSETVLDFMYSLRQQCGDQ--RFPEACTKELVGL 304

  Fly   290 IVLTDYNNRTYRINDVDFGQTPKSTFSCKGRDISFVEYYLTKYNIRIRDHNQPLLISKNRDKALK 354
            |:||.|||:||||:|:.:..||.:||.....:|||..|:.::|.:.|.|.||.||:|  ..|.|.
Zfish   305 IILTKYNNKTYRIDDIAWDHTPNNTFKKGDTEISFKNYFKSQYGLDITDGNQVLLVS--HVKRLG 367

  Fly   355 TNASE---LVVLIPELCRVTGLNAEMRSNFQLMRAMSSYTRMNPKQRTDRLRAFNHRLQNTPESV 416
            .:...   ..:|:||.|.:|||..:||::|.:|:.::|:||::|:||..|:......:....:..
Zfish   368 PSGRPPPGPAMLVPEFCYLTGLTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNGDVQ 432

  Fly   417 KVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGKVPAGE----NADWQRHFRDQRMLTTPSDGLDR 477
            ..|..|.:..:..:..:.||::..:.|: ..|:  |.|    .|||.:..|...:::..|  ||.
Zfish   433 NELTTWGLSFENKLLSLNGRVLPSERII-QGGR--AFEYNPWTADWSKEMRGLPLISCMS--LDN 492

  Fly   478 WAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSPQEFIIYDDRTGTYVRAMDDCVRSDPKLILCL 542
            |.:...:||:...::||.:|.:.:..||:|::. ...|.|:||..:.:||:...|..:.::::.:
Zfish   493 WLMFYTRRNADVAQSLLQTLNKVSGPMGIRMQR-AVMIEYEDRQESLLRALQQNVARETQMVVVI 556

  Fly   543 VPNDNAERYSSIKKRGYVDRAVPTQVVTLKT-TKNRSLMSIATKIAIQLNCKLGYTPWMIELPLS 606
            :|.:..::|..:||...||...|:|.|..:| :|.::||::|||||:|:|||:|...|.:|:||.
Zfish   557 LPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWSVEIPLR 621

  Fly   607 GLMTIGFDIAKSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQH 671
            .||.:|.|....|...||:.||::||:: |..|.:||.....:....:.:.|...:..||:.|..
Zfish   622 QLMIVGIDCYHDTAAGKRSIGAMVASLN-QGMSRWFSKCVLQNRGQEIIDALKGSLQGALKAYLK 685

  Fly   672 EHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIEKLKT---EYARVQLSPPQLAYIVVTRSMNTR 733
            .:..|||||:.|||||..|.|:.:.::||..|::.:||   :|      .|:|:.:||.:.:::|
Zfish   686 YNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDY------EPKLSVVVVKKRISSR 744

  Fly   734 FF--LNGQ--NPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSMGLSPEKMQKLT 794
            ||  ::|:  ||||||::|..:|.||.|||::|||.||.|.|:||.|||::.:.||.|:.||:||
Zfish   745 FFARIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDNSGLKPDHMQRLT 809

  Fly   795 YKMCHLYYNWSGTTRVPAVCQYAKKLATLVGTNLHSIPQNALEKKFYYL 843
            ||:||:||||.|..||||.||||.|||.|||.::|..|...|:...|||
Zfish   810 YKLCHMYYNWQGIVRVPAPCQYAHKLAFLVGQSIHKEPNMNLDDFLYYL 858

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
piwi	NP_001285825.1	ArgoN	91..201	CDD:293095	34/109 (31%)
		PAZ	262..396	CDD:198017	50/140 (36%)
		Piwi_piwi-like_Euk	382..826	CDD:240016	165/455 (36%)
piwil1	NP_899181.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..56		20/71 (28%)
		GAGE	17..104	CDD:283487	17/90 (19%)
		ArgoN	104..203	CDD:293095	30/98 (31%)
		PAZ	275..412	CDD:198017	50/140 (36%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000250\|UniProtKB:Q9JMB7	314..316		1/1 (100%)
		Piwi_piwi-like_Euk	398..841	CDD:240016	165/455 (36%)
		MID region. /evidence=ECO:0000250\|UniProtKB:Q9JMB7	476..612		43/138 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580668
Domainoid	1	1.000	146	1.000	Domainoid score	I4499
eggNOG	1	0.900	-	-		E1_KOG1042
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54391
OrthoDB	1	1.010	-	-		D220258at2759
OrthoFinder	1	1.000	-	-		FOG0003636
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100694
Panther	1	1.100	-	-	O	PTHR22891
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X548
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	1	1.500	-	-
	13	13.220