DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment piwi and AGO3

DIOPT Version :9

Sequence 1:	NP_001285825.1	Gene:	piwi / 34521	FlyBaseID:	FBgn0004872	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_001036627.2	Gene:	AGO3 / 3355150	FlyBaseID:	FBgn0250816	Length:	867	Species:	Drosophila melanogaster

Alignment Length:	862	Identity:	274/862 - (31%)
Similarity:	445/862 - (51%)	Gaps:	85/862 - (9%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 GRGRRR---PLNEDDSSTSRGSGDGPRVKVFRGSSSGDPRADPRIEASRERRALEEAPRREGGPT 67
            ||||.|   .|..||.::::          |..|.|                 .|...::..||
  Fly    67 GRGRARLIDTLKTDDHTSNQ----------FITSES-----------------KENITKKTKGP- 103

  Fly    68 ERKPWGDQYDYLNTRPAELVSKK----------GTDGVPVMLQTNFFRLKTKPEWRIVHYHVEFE 122
                           .:|.::.:          |:.|..|.:..|:.:|.|.....:.:|.|.|.
  Fly   104 ---------------ESEAIASENGLFFPDLIYGSKGSSVNIYCNYLKLTTDESKGVFNYEVRFF 153

  Fly   123 PSIENPRVRMGVLSNHANLLGSGYLFDGLQLFTTRKFEQEITVLSGKSKLDIEYKISI---KFVG 184
            |.|::..:|:..|::|.:.||....|||..|:.......::||...|:: |:|.:|.|   |...
  Fly   154 PPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYLPILLPNKMTVFISKAE-DVELQIRILYKKKEE 217

  Fly   185 FISCAEPRFLQVLNLILRRSMKGLNLELVGRNLFDP-RAKIEIREFKMELWPGYETSIRQHEKDI 248
            ..:|.     |:.|::..|.||.||.....|..||| |.|| |...|:|:||||.|::.:::..:
  Fly   218 MRNCT-----QLYNILFDRVMKVLNYVKFDRKQFDPSRPKI-IPLAKLEVWPGYVTAVDEYKGGL 276

  Fly   249 LLGTEITHKVMRTETIYD-IMRRCSHNPARHQDEVRVNVLDLIVLTDYNNRTYRINDVDFGQTPK 312
            :|..:::|:::..:|:.: ::.....|...:|:..|..::..||||.||||||:|||:.|.|.|.
  Fly   277 MLCCDVSHRILCQKTVLEMLVDLYQQNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPT 341

  Fly   313 STFSCKGRDISFVEYYLTKYNIRIRDHNQPLLISKNRDKALKTNASEL-VVLIPELCRVTGLNAE 376
            ..|..|....|:||||...:||.|:|.||||:.|..:.:.:......| ..||||||.:|||..|
  Fly   342 CQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIKKSRGIPAERENLQFCLIPELCYLTGLRDE 406

  Fly   377 MRSNFQLMRAMSSYTRMNPKQRTDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQ 441
            :||:.:|||.::::||::|.||...|..|...:.|||.:.::|..|.:.|..|..::.||.:..:
  Fly   407 VRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIE 471

  Fly   442 NIVFHNGKVPAGENADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGL 506
            .|.|....|.||.:|::.:|.....||....  |.:|.:|..:.......:|||::.:|...:|:
  Fly   472 QIYFSKISVSAGRSAEFSKHAVTNEMLKVVH--LSKWIIIHLRNYRQAATSLLDNMKQACESLGM 534

  Fly   507 RIRSPQEFIIYDDRTGTYVRAMDDCVRSDPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTL 571
            .|.:|....:..||...|::|:...:..:.::::|:..|...:||::|||....:..:|:||:..
  Fly   535 NISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINA 599

  Fly   572 KTTKN-RSLMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDL 635
            ||.:| ..:.|:..||.:|:|||||.:.|.:::|...:|..|.|......:|..:..|.:||::.
  Fly   600 KTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINS 664

  Fly   636 QQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEV 700
            ..:..|...|.:....::: |.|......||:.|:..:.|||:.|:.||||:..|.|.....:|:
  Fly   665 SYSQWYSKAVVQTKREEIV-NGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEI 728

  Fly   701 KDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFF----LNGQNPPPGTIVDDVITLPERYDFYL 761
                    .::..|..:..:::||||.:.:|||.|    ::.:||.|||:||..||....|||:|
  Fly   729 --------PQFEMVCGNRIKISYIVVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFL 785

  Fly   762 VSQQVRQGTVSPTSYNVLYSSMGLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGT 826
            |||.||||||:||.|.||.......|:.:|||:||:|.|||||:||.|:||.|.||.|||.|:|.
  Fly   786 VSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQ 850

  Fly   827 NLHSIPQNALEKKFYYL 843
            ::......||.:|.:||
  Fly   851 SIQRDVAEALSEKLFYL 867

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
piwi	NP_001285825.1	ArgoN	91..201	CDD:293095	31/112 (28%)
		PAZ	262..396	CDD:198017	54/135 (40%)
		Piwi_piwi-like_Euk	382..826	CDD:240016	151/448 (34%)
AGO3	NP_001036627.2	PAZ	291..425	CDD:198017	54/133 (41%)
AGO3	NP_001036627.2	Piwi_piwi-like_Euk	412..850	CDD:240016	151/448 (34%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45449121
Domainoid	1	1.000	84	1.000	Domainoid score	I5262
eggNOG	1	0.900	-	-		E1_KOG1042
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D220258at2759
OrthoFinder	1	1.000	-	-		FOG0003636
OrthoInspector	1	1.000	-	-		otm14694
orthoMCL	1	0.900	-	-		OOG6_100694
Panther	1	1.100	-	-	P	PTHR22891
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X548
	10	9.750