DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Stam and stam2

DIOPT Version :9

Sequence 1:	NP_477448.1	Gene:	Stam / 34505	FlyBaseID:	FBgn0027363	Length:	689	Species:	Drosophila melanogaster
Sequence 2:	NP_001007370.2	Gene:	stam2 / 492497	ZFINID:	ZDB-GENE-041114-64	Length:	509	Species:	Danio rerio

Alignment Length:	581	Identity:	225/581 - (38%)
Similarity:	309/581 - (53%)	Gaps:	108/581 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MGIFGQSSPFDADVEKATSETNTNDNWSLILDVCDKVTTNPRLAKDCLKAVMRRMGHTDPHVVMQ 65
            |.:||| :|||.|||||||||||.::|.||:|:||:|.|.|..|||||:::|:|:.|..|||.||
Zfish     1 MPLFGQ-NPFDQDVEKATSETNTVEDWGLIMDICDRVGTAPNGAKDCLRSIMKRVNHKVPHVAMQ 64

  Fly    66 AITLLDALSNNCGKPLHLEVASRDFETEFRRLLAKAQPKVSLKMRQVLKNWAENDYKNDRELDLI 130
            |:|||.|...|.||..|||:.||:|.:|.|.:|.:|.|||:.|::.::..||| |::.|.:|.||
Zfish    65 ALTLLGACVANSGKIFHLEICSREFASEVRGVLNRAHPKVNEKLKALMAEWAE-DFQKDPQLSLI 128

  Fly   131 PALYAKLRQEGYDFKNLGDKTSKTVAEKAAALPKDPNVVSSQQEEDDIAKAIELSLKENKGSPKT 195
            .|....|::||..|.....::|.|   |.::.|    ..|....:||:||||||||:|.|...:|
Zfish   129 GATIKSLKEEGVTFPTANPQSSST---KPSSTP----ATSRASADDDLAKAIELSLQEQKQQTET 186

  Fly   196 GSVASTGTASAYPSLYPSFAGTGSSAPSAEASSAPAPEPRKVRALYDFEAAEENELTFFAGEIIH 260
            ..:    |..|.|. |.:..|               .|.|||||||||||||:|||||.|||::.
Zfish   187 RPL----TVIADPP-YNTNGG---------------KESRKVRALYDFEAAEDNELTFKAGELVI 231

  Fly   261 VLDDSDPNWWKGYNQRGEGLFPSNFVTADLSVDPERLDINQQHKSAAAAGQRELDSAAALQQKTE 325
            :|||||||||||.|.||.|||||||||.:|:.:|:.:...::                .:..:..
Zfish   232 ILDDSDPNWWKGENHRGVGLFPSNFVTTNLNAEPDPVMYVEK----------------TVVPEEP 280

  Fly   326 AAAAAAAAQPVEIDESKIDRLLHLLHEANPEDPSQDSDEMLQLEQEVHQMGPLIDAELERVDRKH 390
            .....|..:||.|||.|:|:.|:||...:|.|.:.|..|:|.||....:|.||||.:|:.:||||
Zfish   281 IVEVKAEPEPVYIDEEKMDKTLYLLQNTDPADATPDGPELLTLEDACEKMNPLIDEKLQEIDRKH 345

  Fly   391 AQLTQLSSDLVDAINLYHTLMRDDRAAAGHFAAAAGGFMPGLAGFPGAALYGAPG--------YP 447
            ::|::|:..:::|:.||:.||.:                        |.||.|..        ||
Zfish   346 SELSELNVKVLEALELYNQLMNE------------------------APLYNAYSKMQSLQGTYP 386

  Fly   448 AA------GGFPPAQNY-----PGMHSM--PYGPVPNPPTSAPSQAPGAP-----------GGYL 488
            ||      .|:.|:..|     |..:|:  ..||:.:.|.|..|.||..|           ..|:
Zfish   387 AAPPHLSMQGYQPSGAYMSAGVPQGYSLTDQAGPLCSMPPSINSVAPSQPAQPSYISGPMNAPYV 451

  Fly   489 GQ-PQGQVPLPYQNGHAAQMNSLP-AHLTQPTGTAPMPQPIYTQPTPVTTQQQQQPLPQHP 547
            .| |.|..|.|.|.|.|..|::.. |:...|..:.||..|  .||.|   ||||....|.|
Zfish   452 NQAPGGPTPYPPQMGVAMDMSAYQNANPGLPPTSYPMSAP--AQPAP---QQQQAAFYQQP 507

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Stam	NP_477448.1	VHS_STAM	10..154	CDD:239625	71/143 (50%)
		SH3_STAM	235..288	CDD:212754	43/52 (83%)
		GAT	340..412	CDD:281166	29/71 (41%)
stam2	NP_001007370.2	VHS_STAM	9..147	CDD:239625	70/138 (51%)
		SH3_STAM	206..259	CDD:212754	43/52 (83%)
		GAT	295..368	CDD:281166	30/72 (42%)
		Forkhead_N	366..479	CDD:254796	33/136 (24%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170574147
Domainoid	1	1.000	149	1.000	Domainoid score	I4384
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	360	1.000	Inparanoid score	I2167
OMA	1	1.010	-	-		QHG52024
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002740
OrthoInspector	1	1.000	-	-		otm24922
orthoMCL	1	0.900	-	-		OOG6_103033
Panther	1	1.100	-	-	LDO	PTHR45929
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R571
SonicParanoid	1	1.000	-	-		X1427
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.930