Protein Alignment Stam and GGA3

DIOPT Version :10

• Sequence 1: NP_477448.1
• Gene: Stam / 34505
• FlyBaseID: FBgn0027363
• Length: 689
• Species: Drosophila melanogaster
• Sequence 2: NP_619525.1
• Gene: GGA3 / 23163
• HGNCID: 17079
• Length: 723
• Species: Homo sapiens
• Alignment Length: 766
• Identity: 153/766 - (19%)
• Similarity: 242/766 - (31%)
• Gaps: 272/766 - (35%)

  Fly     5 GQSSPFDADVEKATSETNTNDNWSLILDVCDKVT---TNPRLAKDCLKAVMRRMGHTDPHVVMQA 66
            |:|  .::.:.|||:.:|..::|..|:..||::.   ..|::|   ::.:..::........:||
Human     6 GES--LESWLNKATNPSNRQEDWEYIIGFCDQINKELEGPQIA---VRLLAHKIQSPQEWEALQA 65

  Fly    67 ITLLDALSNNCGKPLHLEVASRDFETEFRRLLAKAQPKVSLKMRQVLKNWAENDYKNDRELDLIP 131
            :|:|:|...|||:..|.||....|..|   |:....||.
Human    66 LTVLEACMKNCGRRFHNEVGKFRFLNE---LIKVVSPKY-------------------------- 101

  Fly   132 ALYAKLRQEGYDFKNLGDKTSKTVAEKAAALPKDPNVVSSQQEEDDIAKAIELSLKENKGSPKTG 196
                           |||:.|:.|.                      .|.|||........|:..
Human   102 ---------------LGDRVSEKVK----------------------TKVIELLYSWTMALPEEA 129

  Fly   197 SVASTGTASAYPSLYPSFAGTGSSAP--SAEASSAPAPEPRKVRALYDFEAAEENELTFFAGEII 259
            .:     ..||..|  ...|...|.|  ..:.:..|:|.||....::|.|  |:::|      :.
Human   130 KI-----KDAYHML--KRQGIVQSDPPIPVDRTLIPSPPPRPKNPVFDDE--EKSKL------LA 179

  Fly   260 HVLDDSDPNWWKGYNQRGEGLFPSNFVTADLSVDPERLDINQQHKSAAAAGQRELDSAAALQQKT 324
            .:|...:|:                    ||.      :.|:..||.
Human   180 KLLKSKNPD--------------------DLQ------EANKLIKSM------------------ 200

  Fly   325 EAAAAAAAAQPVEIDESKIDRL---LHLLHEAN-------------PEDPSQDSDEML------Q 367
                       |:.||::|.::   ||.|.|.|             .::.|.|.|..|      |
Human   201 -----------VKEDEARIQKVTKRLHTLEEVNNNVRLLSEMLLHYSQEDSSDGDRELMKELFDQ 254

  Fly   368 LEQEVHQMGPLIDAELERVDRKHAQLTQLSSDLVDAINLYHTLMRDD-----------RAAAGHF 421
            .|.:...:..|. :|.|..|.....:.|.|.:|...||.|.|::...           ..:.|:.
Human   255 CENKRRTLFKLA-SETEDNDNSLGDILQASDNLSRVINSYKTIIEGQVINGEVATLTLPDSEGNS 318

  Fly   422 AAAAGGFMPGLAGF----------------PGAALYGAPGYPAAGGFPPAQNYPGMHSMPYG--- 467
            ..:..|.:..||..                |.:.:...|..|.|.| ||............|
Human   319 QCSNQGTLIDLAELDTTNSLSSVLAPAPTPPSSGIPILPPPPQASG-PPRSRSSSQAEATLGPSS 382

  Fly   468 -------------------PVPNPPTSAPSQAPGAPGGYLGQPQGQVPLPY---QNGHAAQMNSL 510
                               |.||.|   |.::.|....:|.|.: |..|.:   :.|.|| ..:.
Human   383 TSNALSWLDEELLCLGLADPAPNVP---PKESAGNSQWHLLQRE-QSDLDFFSPRPGTAA-CGAS 442

  Fly   511 PAHLTQP------TGTAPMPQPIYTQPTPVTTQQQQQPLPQ----------HPQQPPQQQGQQPP 559
            .|.|.||      :..||:|.|.   |.||.  ....|.|.          .|...|:.:...|.
Human   443 DAPLLQPSAPSSSSSQAPLPPPF---PAPVV--PASVPAPSAGSSLFSTGVAPALAPKVEPAVPG 502

  Fly   560 QHP---QNS--YHIDPQSQYAQVPPAVTQIQQPQQPPAPHAYMSPQHQQAPPPQGYQPGPNLYGP 619
            .|.   .||  :|:|...|..:.....:.:.:|...|     :.|....|.|...:..||
Human   503 HHGLALGNSALHHLDALDQLLEEAKVTSGLVKPTTSP-----LIPTTTPARPLLPFSTGP----- 557

  Fly   620 NGSPAVVNPQSYMSPQQQQQQHPP--PHDQFSQLHQQMAAMSMAGGPPVGA 668
             ||| :..|.|:     |.|..||  |....:.:|..:.::..:...||.|
Human   558 -GSP-LFQPLSF-----QSQGSPPKGPELSLASIHVPLESIKPSSALPVTA 601

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Stam	NP_477448.1	VHS_STAM	10..141	CDD:340770	26/133 (20%)
		SH3_STAM	235..288	CDD:212754	7/52 (13%)
		GAT_STAM	337..413	CDD:410593	25/97 (26%)
		PHA03247	<436..526	CDD:223021	28/120 (23%)
GGA3	NP_619525.1	Binds to ARF1 (in long isoform)	1..313		85/448 (19%)
		VHS_GGA3	6..146	CDD:340805	42/217 (19%)
		GGA_N-GAT	169..207	CDD:465702	14/100 (14%)
		GAT_GGA3	213..299	CDD:410587	22/86 (26%)
		Unstructured hinge	299..593		65/321 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	339..384		8/45 (18%)
		DXXLL. /evidence=ECO:0000269|PubMed:12060753	391..395		0/3 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	428..464		10/36 (28%)
		Alpha_adaptinC2	601..715	CDD:197886	1/1 (100%)