Protein Alignment Nos and cysJ

DIOPT Version :10

• Sequence 1: NP_523541.2
• Gene: Nos / 34495
• FlyBaseID: FBgn0011676
• Length: 1349
• Species: Drosophila melanogaster
• Sequence 2: NP_417244.1
• Gene: cysJ / 947239
• ECOCYCID: EG10191
• Length: 599
• Species: Escherichia coli
• Alignment Length: 672
• Identity: 196/672 - (29%)
• Similarity: 289/672 - (43%)
• Gaps: 156/672 - (23%)

  Fly   672 TVLYATETGKSEQYAKQL-CELLGHAFNAQIYCMSDYDISSIEHEALLIVVASTFGNGDPPENGE 735
            |::.|::||.:.:.|:.| .:||....|.::....||....|..|.|||||.||.|.|:|||...
E. coli    65 TIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAV 129

  Fly   736 LFSQELYAMRVQESSEHGLQDSSIGSSKSFMKASSRQEFMKLPLQQVKRIDRWDSLRGSTSDTFT 800
            ...:.|::.:..:
E. coli   130 ALHKFLFSKKAPK---------------------------------------------------- 142

  Fly   801 EETFGPLSNVRFAVFALGSSAYPNFCAFGQYVDNILGELGGERLL-RVAYGDEMCGQEQSFRKWA 864
                  |.|..||||:||.|:|..||..|:..|:.|.|||||||| ||....|.......:|...
E. coli   143 ------LENTAFAVFSLGDSSYEFFCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARV 201

  Fly   865 PEVFKLACETFCLDPEESLSDASLALQNDSL---TVNTVRLVPSANKGSLDSSLSKYHNKKVHCC 926
            .:..|              |.|.:|..:.|:   .||.:...|.:....|.:|||.  |:|:
E. coli   202 VDALK--------------SRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVASLSV--NQKI--- 247

  Fly   927 KAKAKPHNLTRLSEGAKTTMLLEICAPGLEYEPGDHVGIFPANRTELVDGLLNRL-VGVDNP--- 987
                    ..|.||.....:.:::...|:.|:|||.:|::..|...||..|:..| :..|.|
E. coli   248 --------TGRNSEKDVRHIEIDLGDSGMRYQPGDALGVWYQNDPALVKELVELLWLKGDEPVTV 304

  Fly   988 -------DEVLQLQLLKEKQTSNGIFKCWEPHDKIPPDTLRNLLARFFDLTTPPSRQLLTLLAGF 1045
                   :|.||                |  |.::..:| .|::..:..||.  |..||.|:
E. coli   305 EGKTLPLNEALQ----------------W--HFELTVNT-ANIVENYATLTR--SETLLPLV--- 345

  Fly  1046 CEDTADKERLELLVNDSSAYEDWRHWRLPHLLDVLEEFPSCRPPAPLLLAQLTPLQPRFYSISSS 1110
                .||.:|:             |:.....:..:..|...:..|..|:..|.||.||.|||:||
E. coli   346 ----GDKAKLQ-------------HYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASS 393

  Fly  1111 PRRVSDEIHLTVAIVKYRCEDGQGDERYGVCSNYLSG-LRADDELFMFVRSALGFHLPSDRSRPI 1174
            ...|.:|:|:||.:|:|   |.:|..|.|..|::|:. :..:.|:.:|:.....|.||::...|:
E. coli   394 QAEVENEVHVTVGVVRY---DVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPV 455

  Fly  1175 ILIGPGTGIAPFRSFWQEFQVLSDLDPTAKLPKMWLFFGCRNRDVD-LYAEEKAELQKDQILDRV 1238
            |:|||||||||||:|.|  |..:|..|    .|.|||||..:...| ||..|.....||.:|.|:
E. coli   456 IMIGPGTGIAPFRAFMQ--QRAADEAP----GKNWLFFGNPHFTEDFLYQVEWQRYVKDGVLTRI 514

  Fly  1239 FLALSREQAIPKTYVQDLIEQEFDSLYQLIVQERGHIYVCGDVT-MAEHVYQTIRKCIAGKEQKS 1302
            .||.||:|. .|.||||.:.::...|::.| .:..|||||||.. ||:.|.|.:.:.||......
E. coli   515 DLAWSRDQK-EKVYVQDKLREQGAELWRWI-NDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMD 577

  Fly  1303 EAEVETFLLTLRDESRYHEDIF 1324
            ....:.||..||.|.||..|::
E. coli   578 TEAADEFLSELRVERRYQRDVY 599

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nos	NP_523541.2	NOS_oxygenase_euk	216..624	CDD:238410
		Flavodoxin_1	673..863	CDD:425562	52/191 (27%)
		Nitric_oxide_synthase	931..1328	CDD:99799	129/408 (32%)
cysJ	NP_417244.1	cysJ	1..599	CDD:182862	196/670 (29%)