DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nos and nos1

DIOPT Version :9

Sequence 1:	NP_523541.2	Gene:	Nos / 34495	FlyBaseID:	FBgn0011676	Length:	1349	Species:	Drosophila melanogaster
Sequence 2:	NP_571735.1	Gene:	nos1 / 60658	ZFINID:	ZDB-GENE-001101-1	Length:	1431	Species:	Danio rerio

Alignment Length:	1232	Identity:	578/1232 - (46%)
Similarity:	774/1232 - (62%)	Gaps:	92/1232 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly   152 GVGVGQGAGCPPSGSCTASGKSSRELSPSPKNQQQPRKMSQDYRSRAGSFMHLDDEGRSLLMRKP 216
            |||.||.|        ..:..:.|......:....|..::..|..        .|:.:|.....|
Zfish   239 GVGNGQNA--------QLASDNDRVFDDLWRKDNMPTVLNNPYSE--------SDKPQSYERVSP 287

  Fly   217 ---------------MRLKNIEGRPEVYDTLHCKGREILSCSKATCTSSIMNIGNAAVEA---RK 263
                           |::||.|......||||....::..|::..|..|||.....|.|.   |:
Zfish   288 TKAVQNGSPSKCPRFMKVKNWETGALYNDTLHHSSSKVPICTEHVCIGSIMVPNQHAHEPDEDRR 352

  Fly   264 SDLILEHAKDFLEQYFTSIKRTSSTAHETRWKQVRQSIETTGHYQLTETELIYGAKLAWRNSSRC 328
            .:.:|..|.||::||:|||||..|.||..|.::|::.|||:|.|||.:||||||||.||||::||
Zfish   353 KEELLPLATDFIDQYYTSIKRCCSKAHVDRLEEVKKEIETSGTYQLKDTELIYGAKHAWRNAARC 417

  Fly   329 IGRIQWSKLQVFDCRYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQRTDAKHDYRIWNNQLI 393
            :||||||||||||.|..||..|||..|||||||||||||||||||||||:||.|||:|:||:|||
Zfish   418 VGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQKTDGKHDFRVWNSQLI 482

  Fly   394 SYAGYKQADGKIIGDPMNVEFTEVCTKLGWKSKGSEWDILPLVVSANGHDPDYFDYPPELILEVP 458
            .||||||.||.|:|||.:||.||:|.:.|||:....:|:|||::.|||:||:.|:.|.:|:||||
Zfish   483 RYAGYKQPDGSILGDPASVELTEICMQQGWKAPKGRFDVLPLLLQANGNDPELFEIPDDLVLEVP 547

  Fly   459 LTHPKFEWFSDLGLRWYALPAVSSMLFDVGGIQFTATTFSGWYMSTEIGSRNLCDTNRRNMLETV 523
            :.|||||||.:|.|:||.|||||:||.::||::|||..||||||.||||.|:.||::|.|:||.|
Zfish   548 IIHPKFEWFKELNLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDFCDSSRYNILEEV 612

  Fly   524 ALKMQLDTRTPTSLWKDKAVVEMNIAVLHSYQSRNVTIVDHHTASESFMKHFENESKLRNGCPAD 588
            |..|.||||..:|||||:|:||:|||||:|||...|||||||:|:||||||.|||.::|.|||.|
Zfish   613 ATMMGLDTRKTSSLWKDQALVEINIAVLYSYQMSKVTIVDHHSATESFMKHMENEYRVRGGCPGD 677

  Fly   589 WIWIVPPLSGSITPVFHQEMALYYLKPSFEYQDPAWRTHVWKKGRGESKGKKPRRKFNFKQIARA 653
            |:|||||:||||||||||||..|.|.||||||...|.|||||...|....|   |...||::|:|
Zfish   678 WVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGVNGTPTKK---RAIGFKKLAKA 739

  Fly   654 VKFTSKLFGRALSKRIKATVLYATETGKSEQYAKQLCELLGHAFNAQIYCMSDYDISSIEHEALL 718
            |||::.|.|:|::||:|||:|:|||||||:.|||.|||:..|||:|::..|.:||...:|||.|:
Zfish   740 VKFSATLMGQAMAKRVKATILFATETGKSQDYAKTLCEIFKHAFDAKVMSMDEYDTVDLEHETLV 804

  Fly   719 IVVASTFGNGDPPENGELFSQELYAMR-----VQESSEHGLQDSSIGSSKSFMKASSRQEFMKLP 778
            |||.|||||||||||||.|...|..||     |::...:.::.:|:.|.....|:||.:...|..
Zfish   805 IVVTSTFGNGDPPENGEKFGAALMEMRHPTTSVEDRKTYKVRFNSVSSYSDTRKSSSDEPESKAN 869

  Fly   779 LQQVKRIDRWDSLRGSTSDTFTEETFGPLSNVRFAVFALGSSAYPNFCAFGQYVDNILGELGGER 843
            .                      |:.|||:||||:||.|||.|||:||||...||.:..|||.||
Zfish   870 F----------------------ESTGPLANVRFSVFGLGSRAYPHFCAFAHAVDTLFEELGRER 912

  Fly   844 LLRVAYGDEMCGQEQSFRKWAPEVFKLACETFCLDPEESLSDASLALQNDSLTVN-------TVR 901
            :||:..|||:||||:|||.||.:|||.||:.||:.     .|.::...||||..|       ..|
Zfish   913 ILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVG-----DDVNIEKANDSLISNDRSWKKSKFR 972

  Fly   902 LVPSANKGSLDSSLSKYHNKKVHCCKAKAKPHNLTRLSEGAKTTMLLEICA---PGLEYEPGDHV 963
            :..:|...:|..:|...|.|||:..|. .:..|| :.:...:.|:.:.:.|   ..|:|.||||:
Zfish   973 MTYTAEAPTLTQALYSIHKKKVYGAKV-VQRQNL-QSARSNRLTIFVRLDANNHESLKYLPGDHL 1035

  Fly   964 GIFPANRTELVDGLLNRLVGVDNPDEVLQLQLLKEKQTSNGIFKCWEPHDKIPPDTLRNLLARFF 1028
            |:||.|..:||..|:.:|......:::::::.|:|:.|:.|:...|....:|||.|:......|.
Zfish  1036 GVFPGNNEDLVTTLIEKLEDAPPVNQIVKVEFLEERNTALGVISNWTDESRIPPCTIYQAFKYFL 1100

  Fly  1029 DLTTPPSRQLLTLLAGFCEDTADKERLELLVNDSSAYEDWRHWRLPHLLDVLEEFPSCRPPAPLL 1093
            |:|||||..||...|....:...::|||:|......||:|:.:..|.:::|||||||.:.|:.||
Zfish  1101 DITTPPSPVLLQQFAPLATNEKQRKRLEVLSKGLQEYEEWKWYNNPTMVEVLEEFPSLQIPSTLL 1165

  Fly  1094 LAQLTPLQPRFYSISSSPRRVSDEIHLTVAIVKYRCEDGQGDERYGVCSNYLSGLRADDELFMFV 1158
            |.||..||||:|||||||.....|||||||:|.||..||:|...:||||::|:.|...|.:..||
Zfish  1166 LTQLPLLQPRYYSISSSPDLYPGEIHLTVAVVSYRPRDGEGPIHHGVCSSWLNSLDEGDTVPCFV 1230

  Fly  1159 RSALGFHLPSDRSRPIILIGPGTGIAPFRSFWQEFQVLSDLDPTA-KLPKMWLFFGCRNRDVD-L 1221
            |.|..|.:|.|...|.|||||||||||||:|||:.|.  |::... |...|.|.||||...:| :
Zfish  1231 RGAPSFRMPKDSQVPCILIGPGTGIAPFRAFWQQRQY--DVENKGIKACPMILVFGCRQSQIDHI 1293

  Fly  1222 YAEEKAELQKDQILDRVFLALSREQAIPKTYVQDLI-EQEFDSLYQLIVQERGHIYVCGDVTMAE 1285
            |.|:..:.:..::...::.|.|||...||.||||:: ||..:::|:.:.:|.||||||||||||.
Zfish  1294 YKEQTIQAKNKEVFKELYTAYSREPGRPKKYVQDVLREQLSETVYKCLREEGGHIYVCGDVTMAG 1358

  Fly  1286 HVYQTIRKCIAGKEQKSEAEVETFLLTLRDESRYHEDIFGITLRTAEIHTKSRATARIRMAS 1347
            .|.:|:::........|..:...::..||||:||||||||:||||.|:      |.|:|..|
Zfish  1359 DVLKTVQQIFKLHGNMSLEDAGFYISKLRDENRYHEDIFGVTLRTCEV------TNRLRSES 1414

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nos	NP_523541.2	NOS_oxygenase_euk	216..624	CDD:238410	253/425 (60%)
		CysJ	669..1323	CDD:223446	280/671 (42%)
		Flavodoxin_1	673..863	CDD:278677	91/194 (47%)
		Nitric_oxide_synthase	931..1328	CDD:99799	166/402 (41%)
nos1	NP_571735.1	PDZ_signaling	15..90	CDD:238492
		NOS_oxygenase_euk	302..713	CDD:238410	252/410 (61%)
		CysJ	756..1396	CDD:223446	280/670 (42%)
		Flavodoxin_1	759..932	CDD:278677	91/194 (47%)
		Nitric_oxide_synthase	998..1401	CDD:99799	167/406 (41%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	524	1.000	Domainoid score	I281
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H37327
Inparanoid	1	1.050	1098	1.000	Inparanoid score	I229
OMA	1	1.010	-	-		QHG46820
OrthoDB	1	1.010	-	-		D56017at33208
OrthoFinder	1	1.000	-	-		FOG0001418
OrthoInspector	1	1.000	-	-		otm25886
orthoMCL	1	0.900	-	-		OOG6_103176
Panther	1	1.100	-	-	O	PTHR19384
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X3122
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.980