Protein Alignment Nos and nos2a

DIOPT Version :9

• Sequence 1: NP_523541.2
• Gene: Nos / 34495
• FlyBaseID: FBgn0011676
• Length: 1349
• Species: Drosophila melanogaster
• Sequence 2: NP_001098407.1
• Gene: nos2a / 404036
• ZFINID: ZDB-GENE-040305-1
• Length: 1079
• Species: Danio rerio
• Alignment Length: 1157
• Identity: 493/1157 - (42%)
• Similarity: 690/1157 - (59%)
• Gaps: 123/1157 - (10%)

  Fly   204 LDDEGRSLLMRKP----------MRLKNIEGRPEVYDTLHCKGREILSCSKATCTSSIMN----- 253
            :::|.:::..::|          ::|:|.:......|:||.|..:...|:..||..||||
Zfish    10 IENEKQTVQSKRPVDRPIRCPFAVQLRNYKDGSTHQDSLHHKAAKNTPCNSKTCEGSIMNPKPLI 74

  Fly   254 IGNAAVEARKSDLILEHAKDFLEQYFTSIKRTSSTAHETRWKQVRQSIETTGHYQLTETELIYGA 318
            .|.:.:.....|: |..|:||::||:.|:|...||..:.|..::...|..||.|.||..||.:||
Zfish    75 RGPSKITPSPDDM-LSQAEDFIDQYYKSLKIPGSTEQQARIDRIALEITLTGTYSLTTEELAFGA 138

  Fly   319 KLAWRNSSRCIGRIQWSKLQVFDCRYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQRTDAKH 383
            |.||||:.|||||||||.||:||.|...|...||.|:|.|:|||||.||||||||:||||||..|
Zfish   139 KQAWRNAPRCIGRIQWSNLQLFDARDCKTAEDMFHALCTHLKYATNGGNLRSAITVFPQRTDEGH 203

  Fly   384 DYRIWNNQLISYAGYKQADGKIIGDPMNVEFTEVCTKLGWKSKGSEWDILPLVVSANGHDPDYFD 448
            |:|:||.|||.||||:..||.:||||..|:||:||.:||||.|...:|:||||:.|||.||:.|:
Zfish   204 DFRVWNGQLIKYAGYQMDDGSVIGDPAGVDFTQVCIQLGWKPKYGRFDVLPLVLQANGEDPEVFE 268

  Fly   449 YPPELILEVPLTHPKFEWFSDLGLRWYALPAVSSMLFDVGGIQFTATTFSGWYMSTEIGSRNLCD 513
            .|||||||||:.||::|||.||.|:|||:|||::||.:|||::|||..|:||||.||||.|:.||
Zfish   269 IPPELILEVPIEHPEYEWFKDLDLKWYAIPAVANMLMEVGGLEFTACPFNGWYMGTEIGVRDFCD 333

  Fly   514 TNRRNMLETVALKMQLDTRTPTSLWKDKAVVEMNIAVLHSYQSRNVTIVDHHTASESFMKHFENE 578
            :.|.|:||.|...|.|:||..:|||||:|:|.:|:||:||||...|||.|||:||||||:|.|.|
Zfish   334 SQRYNILERVGQLMGLETRKLSSLWKDQALVAINVAVIHSYQKNKVTITDHHSASESFMQHLETE 398

  Fly   579 SKLRNGCPADWIWIVPPLSGSITPVFHQEMALYYLKPSFEYQDPAWRTHVWKKGRGESKGKKPRR 643
            .:||.||||||:|:|||:|||:|||:|||:..|.|.|.|.||...|.||.||   .|.||.: |.
Zfish   399 VRLRGGCPADWVWLVPPMSGSLTPVYHQELLNYILSPFFYYQTDPWLTHKWK---DEKKGLR-RC 459

  Fly   644 KFNFKQIARAVKFTSKLFGRALSKRIKATVLYATETGKSEQYAKQLCELLGHAFNAQIYCMSDYD 708
            |.:||.:.|||.|:..|....|:||::.|||||||||||..:||:|..:|..||::::.||.:||
Zfish   460 KVSFKGLIRAVFFSQTLIKAVLAKRVRCTVLYATETGKSLSFAKKLNTMLNRAFDSRLVCMEEYD 524

  Fly   709 ISSIEHEALLIVVASTFGNGDPPENGELFSQELYAMRVQESSEHGLQDSSIGSSKSFMKASSRQE 773
            .|.:|.|.||:||.||||||:.|.||..|.::|::
Zfish   525 FSELETETLLMVVTSTFGNGESPGNGVTFRKQLFS------------------------------ 559

  Fly   774 FMKLPLQQVKRIDRWDSLRGSTSDTFTEETFGPLSNVRFAVFALGSSAYPNFCAFGQYVDNILGE 838
                 ||:||                        :.:|:.||.|||..||.||||...||:.|.|
Zfish   560 -----LQKVK------------------------NTIRYGVFGLGSRVYPQFCAFAHAVDDKLAE 595

  Fly   839 LGGERLLRVAYGDEMCGQEQSFRKWAPEVFKLACETFCL--------DPEESLSDASLALQNDSL 895
            ||.:|:.....|||:.||:::|..||...||.|||.|.:        :..|........:|.||.
Zfish   596 LGAKRMTATGEGDELNGQDEAFSAWACTAFKDACEEFNIKKQLKAVEEQTEGWDPQRYRVQQDSC 660

  Fly   896 TVNTVRLVPSANKGSLDSSLSKYHNKKVHCCKAKAKPHNLTR-----LSEGAKTTMLLEICAPG- 954
            |::.:            ::||..|:|.|.       |..|.|     .::.:::|:|:|:...|
Zfish   661 TLDPI------------TALSALHSKTVF-------PMTLKRRQKLQSAQSSRSTILVELKTDGN 706

  Fly   955 ---LEYEPGDHVGIFPANRTELVDGLLNRLVGVDNPDEVLQLQLLKEKQTSNGIFKCWEPHDKIP 1016
               |::.|||||||||.|.:|||.|::..|......::.|:|:.|.|.....   |.|:..:::|
Zfish   707 TDALKFAPGDHVGIFPENSSELVHGIVKHLPDATPDNQSLRLETLSESSHEG---KRWQTDERLP 768

  Fly  1017 PDTLRNLLARFFDLTTPPSRQLLTLLAGFCEDTADKERLELLVNDSSAYEDWRHWRLPHLLDVLE 1081
            ..||...|..|.|:|||||:.||..|:...:...|::|||.|.:|...|..|:.:..|..|:|||
Zfish   769 ACTLVQALTYFLDVTTPPSQSLLRKLSTAADQEEDRKRLEALASDFQEYSTWKDFYRPTFLEVLE 833

  Fly  1082 EFPSCRPPAPLLLAQLTPLQPRFYSISSSPRRVSDEIHLTVAIVKYRCEDGQGDERYGVCSNYLS 1146
            ||||.:.|:..:|:|:..|:||.||:|||.....:|:|||:::|:|:.:.|.|...:|.||.:.:
Zfish   834 EFPSLKVPSAFILSQIPLLKPRLYSVSSSLDHHPNELHLTISVVEYQTQGGAGPMHFGTCSTWFN 898

  Fly  1147 GLRADDELFMFVRSALGFHLPSDRSRPIILIGPGTGIAPFRSFWQEFQVLSDLDPTA-KLPKMWL 1210
            .::....:..||..:.|||||.|.|.|::|||.|:||||||||||  |...|:..|. ::..|.|
Zfish   899 TIKRGHTVPCFVHRSGGFHLPEDPSTPVLLIGAGSGIAPFRSFWQ--QRTQDVKKTGQQISPMTL 961

  Fly  1211 FFGCRNRDVD-LYAEEKAELQKDQILDRVFLALSREQAIPKTYVQDLI-EQEFDSLYQLIVQERG 1273
            .||||:.:.| ||..|..:|:.:.||..|..|.||:...||.||||:: ||..:...:::.:..|
Zfish   962 LFGCRDSETDHLYKVETLKLRLNGILGNVNTAYSRQPGKPKMYVQDVLKEQLVEKALEVLHKSAG 1026

  Fly  1274 HIYVCGDVTMAEHVYQTIRKCIAGKEQKSEAEVETFLLTLRDESRYHEDIFG 1325
            |:||||.:.||:.|.||::..:......||.|...::..|:.|.||||||||
Zfish  1027 HLYVCGGMNMAKDVAQTLKDILVNNTIMSEVEAGEYMDMLKVEKRYHEDIFG 1078

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nos	NP_523541.2	NOS_oxygenase_euk	216..624	CDD:238410	228/422 (54%)
		CysJ	669..1323	CDD:223446	240/673 (36%)
		Flavodoxin_1	673..863	CDD:278677	67/189 (35%)
		Nitric_oxide_synthase	931..1328	CDD:99799	158/407 (39%)
nos2a	NP_001098407.1	NOS_oxygenase_euk	33..444	CDD:238410	227/411 (55%)
		CysJ	469..1076	CDD:223446	247/689 (36%)
		Flavodoxin_1	489..620	CDD:278677	67/189 (35%)
		FNR_like	680..1079	CDD:297884	157/404 (39%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	524	1.000	Domainoid score	I281
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG46820
OrthoDB	1	1.010	-	-		D56017at33208
OrthoFinder	1	1.000	-	-		FOG0001418
OrthoInspector	1	1.000	-	-		otm25886
orthoMCL	1	0.900	-	-		OOG6_103176
Panther	1	1.100	-	-	O	PTHR19384
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4692
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.960