DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nos and Nos1

DIOPT Version :9

Sequence 1:	NP_523541.2	Gene:	Nos / 34495	FlyBaseID:	FBgn0011676	Length:	1349	Species:	Drosophila melanogaster
Sequence 2:	XP_038944987.1	Gene:	Nos1 / 24598	RGDID:	3184	Length:	1532	Species:	Rattus norvegicus

Alignment Length:	1372	Identity:	621/1372 - (45%)
Similarity:	841/1372 - (61%)	Gaps:	133/1372 - (9%)

- Green bases have known domain annotations that are detailed below.

  Fly    48 TQQQNSRKIKTQATPTLNGNGLLSGNPNGGGGDSSPSHEVDHPGGAQGAQAAGGL---PSSSGTP 109
            :.|.::.|.::.|...:.|.|      ||      |.|...|..||.....|.|:   |:...|
  Rat   136 SHQPSASKDQSLAVDRVTGLG------NG------PQHAQGHGQGAGSVSQANGVAIDPTMKST- 187

  Fly   110 LRHHKRASISTASPPIRERRGTNTSIVVELD------GSGSGSGSGGG---------GVGV---- 155
                 :|::...        |.:..::.|::      .|||.:.:.||         |:.|
  Rat   188 -----KANLQDI--------GEHDELLKEIEPVLSILNSGSKATNRGGPAKAEMKDTGIQVDRDL 239

  Fly   156 -GQGAGCPPSGSCTAS------GK-----------SSRELSPSPKNQQQPRKMSQDYRSRAGSFM 202
             |:....||.|.....      ||           |.:|.||: ..:|.|.|....  ||...|
  Rat   240 DGKSHKAPPLGGDNDRVFNDLWGKDNVPVVLNNPYSEKEQSPT-SGKQSPTKNGSP--SRCPRF- 300

  Fly   203 HLDDEGRSLLMRKPMRLKNIEGRPEVYDTLHCKGREILSCSKATCTSSIM---NIGNAAVEARKS 264
                          :::||.|....:.||||.|......|::..|..|||   .......:.|..
  Rat   301 --------------LKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPSQHTRKPEDVRTK 351

  Fly   265 DLILEHAKDFLEQYFTSIKRTSSTAHETRWKQVRQSIETTGHYQLTETELIYGAKLAWRNSSRCI 329
            |.:...||:||:||::||||..|.||..|.::|.:.||:|..|||.:||||||||.||||:|||:
  Rat   352 DQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCV 416

  Fly   330 GRIQWSKLQVFDCRYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQRTDAKHDYRIWNNQLIS 394
            ||||||||||||.|..||..|||..||||:|||||||||||||||||||||.|||:|:||:|||.
  Rat   417 GRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIR 481

  Fly   395 YAGYKQADGKIIGDPMNVEFTEVCTKLGWKSKGSEWDILPLVVSANGHDPDYFDYPPELILEVPL 459
            ||||||.||..:|||.||:|||:|.:.|||:....:|:|||::.|||:||:.|..||||:||||:
  Rat   482 YAGYKQPDGSTLGDPANVQFTEICIQQGWKAPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPI 546

  Fly   460 THPKFEWFSDLGLRWYALPAVSSMLFDVGGIQFTATTFSGWYMSTEIGSRNLCDTNRRNMLETVA 524
            .||||:||.||||:||.|||||:||.::||::|:|..||||||.||||.|:.||.:|.|:||.||
  Rat   547 RHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVA 611

  Fly   525 LKMQLDTRTPTSLWKDKAVVEMNIAVLHSYQSRNVTIVDHHTASESFMKHFENESKLRNGCPADW 589
            .||.||.|..:|||||:|:||:|||||:|:||..|||||||:|:|||:||.|||.:.|.||||||
  Rat   612 KKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADW 676

  Fly   590 IWIVPPLSGSITPVFHQEMALYYLKPSFEYQDPAWRTHVWKKGRGESKGKKPRRKFNFKQIARAV 654
            :|||||:||||||||||||..|.|.||||||...|.|||||   |.:.....||...||::|.||
  Rat   677 VWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWK---GTNGTPTKRRAIGFKKLAEAV 738

  Fly   655 KFTSKLFGRALSKRIKATVLYATETGKSEQYAKQLCELLGHAFNAQIYCMSDYDISSIEHEALLI 719
            ||::||.|:|::||:|||:|||||||||:.|||.|||:..|||:|:...|.:|||..:|||||::
  Rat   739 KFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVL 803

  Fly   720 VVASTFGNGDPPENGELFSQELYAMR----VQESSEH----------GLQDSSIGSSKSFMKAS- 769
            ||.|||||||||||||.|...|..||    |||..::          |...|.:.|....:.|:
  Rat   804 VVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPSLSHVDSEAHSLVAAR 868

  Fly   770 -SRQEFMKLPLQQVKRIDRWDSLRGS-----TSDTFTEETFGPLSNVRFAVFALGSSAYPNFCAF 828
             |:....|:....|....  ||.:.|     ..|.|  |:.|||:||||:||.|||.|||:||||
  Rat   869 DSQHRSYKVRFNSVSSYS--DSRKSSGDGPDLRDNF--ESTGPLANVRFSVFGLGSRAYPHFCAF 929

  Fly   829 GQYVDNILGELGGERLLRVAYGDEMCGQEQSFRKWAPEVFKLACETFCLDPEESLSDASLAL-QN 892
            |..||.:|.||||||:|::..|||:||||::||.||.:|||.||:.||:..:.::..|:.:| .|
  Rat   930 GHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISN 994

  Fly   893 D-SLTVNTVRLVPSANKGSLDSSLSKYHNKKVHCCKAKAKPHNLTRLSEGAKTTMLLEICAPG-- 954
            | |...|..||...|....|...||..|.|:|...:..:: .|| :..:.:::|:.:.:...|
  Rat   995 DRSWKRNKFRLTYVAEAPDLTQGLSNVHKKRVSAARLLSR-QNL-QSPKSSRSTIFVRLHTNGNQ 1057

  Fly   955 -LEYEPGDHVGIFPANRTELVDGLLNRLVGVDNPDEVLQLQLLKEKQTSNGIFKCWEPHDKIPPD 1018
             |:|:||||:|:||.|..:||:.|:.||......:.|:::::|:|:.|:.|:...|:...::||.
  Rat  1058 ELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPC 1122

  Fly  1019 TLRNLLARFFDLTTPPSRQLLTLLAGFCEDTADKERLELLVNDSSAYEDWRHWRLPHLLDVLEEF 1083
            |:......:.|:||||:...|...|....:..:|:||.:|......||:|:..:.|.:::|||||
  Rat  1123 TIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEF 1187

  Fly  1084 PSCRPPAPLLLAQLTPLQPRFYSISSSPRRVSDEIHLTVAIVKYRCEDGQGDERYGVCSNYLSGL 1148
            ||.:.||.|||.||:.||||:|||||||....||:|||||||.|...||:|...:||||::|:.:
  Rat  1188 PSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRI 1252

  Fly  1149 RADDELFMFVRSALGFHLPSDRSRPIILIGPGTGIAPFRSFWQEFQVLSDLDPTAKLP-KMWLFF 1212
            :|||.:..|||.|..||||.:...|.||:||||||||||||||:.|.  |:......| .|.|.|
  Rat  1253 QADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQF--DIQHKGMNPCPMVLVF 1315

  Fly  1213 GCRNRDVD-LYAEEKAELQKDQILDRVFLALSREQAIPKTYVQDLIEQEF-DSLYQLIVQERGHI 1275
            |||...:| :|.||..:.:...:...::.|.|||...||.||||:::::. :|:|:.:.::.|||
  Rat  1316 GCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHI 1380

  Fly  1276 YVCGDVTMAEHVYQTIRKCIAGKEQKSEAEVETFLLTLRDESRYHEDIFGITLRTAEIHTKSRAT 1340
            |||||||||..|.:.|::.:..:.:.||.:...|:..|||::||||||||:||||.|:      |
  Rat  1381 YVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEV------T 1439

  Fly  1341 ARIRMAS 1347
            .|:|..|
  Rat  1440 NRLRSES 1446

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nos	NP_523541.2	NOS_oxygenase_euk	216..624	CDD:238410	252/410 (61%)
		CysJ	669..1323	CDD:223446	292/682 (43%)
		Flavodoxin_1	673..863	CDD:278677	102/210 (49%)
		Nitric_oxide_synthase	931..1328	CDD:99799	167/402 (42%)
Nos1	XP_038944987.1	PDZ_signaling	15..98	CDD:238492
		NOS_oxygenase_euk	300..711	CDD:238410	253/425 (60%)
		Flavodoxin_1	757..964	CDD:395199	102/210 (49%)
		Nitric_oxide_synthase	1030..1433	CDD:99799	167/406 (41%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	526	1.000	Domainoid score	I283
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H37327
Inparanoid	1	1.050	1111	1.000	Inparanoid score	I213
OMA	1	1.010	-	-		QHG46820
OrthoDB	1	1.010	-	-		D56017at33208
OrthoFinder	1	1.000	-	-		FOG0001418
OrthoInspector	1	1.000	-	-		otm46194
orthoMCL	1	0.900	-	-		OOG6_103176
Panther	1	1.100	-	-	O	PTHR19384
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X3122
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.980