DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nos and Nos1

DIOPT Version :9

Sequence 1:	NP_523541.2	Gene:	Nos / 34495	FlyBaseID:	FBgn0011676	Length:	1349	Species:	Drosophila melanogaster
Sequence 2:	NP_032738.1	Gene:	Nos1 / 18125	MGIID:	97360	Length:	1429	Species:	Mus musculus

Alignment Length:	1329	Identity:	612/1329 - (46%)
Similarity:	827/1329 - (62%)	Gaps:	129/1329 - (9%)

- Green bases have known domain annotations that are detailed below.

  Fly    78 GGDSSPSHEVDHPGGAQG-AQAAGGLPSSSGT---PLRHHKRASISTASPPIRERRGTNTSIVVE 138
            |..:.|.|       ||| .|.||.:..::|.   |...:.:|::..:        |....::.|
Mouse   154 GPSNGPQH-------AQGRGQGAGSVSQANGVAIDPTMKNTKANLQDS--------GEQDELLKE 203

  Fly   139 LD-------GSGSGSGSGG--------GGVGV-----GQGAGCPPSGSCTASGKSSREL------ 177
            ::       |.|.....||        .|:.|     |:....||.|     |::.|..
Mouse   204 IEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLG-----GENDRVFNDLWGK 263

  Fly   178 --------SPSPKNQQQPRKMSQD-----YRSRAGSFMHLDDEGRSLLMRKPMRLKNIEGRPEVY 229
                    :|..:|:|.|....|.     ..||...|               :::||.|....:.
Mouse   264 GNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRF---------------LKVKNWETDVVLT 313

  Fly   230 DTLHCKGREILSCSKATCTSSIMNIGN---AAVEARKSDLILEHAKDFLEQYFTSIKRTSSTAHE 291
            ||||.|......|::..|..|||...:   .:.:.|..|.:...||:||:||::||||..|.||.
Mouse   314 DTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHM 378

  Fly   292 TRWKQVRQSIETTGHYQLTETELIYGAKLAWRNSSRCIGRIQWSKLQVFDCRYVTTTSGMFEAIC 356
            .|.::|.:.||:|..|||.:||||||||.||||:|||:||||||||||||.|..||..|||..||
Mouse   379 DRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYIC 443

  Fly   357 NHIKYATNKGNLRSAITIFPQRTDAKHDYRIWNNQLISYAGYKQADGKIIGDPMNVEFTEVCTKL 421
            ||:|||||||||||||||||||||.|||:|:||:|||.||||||.||..:|||.||||||:|.:.
Mouse   444 NHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQ 508

  Fly   422 GWKSKGSEWDILPLVVSANGHDPDYFDYPPELILEVPLTHPKFEWFSDLGLRWYALPAVSSMLFD 486
            |||.....:|:|||::.|||:||:.|..||||:||||:.||||:||.||||:||.|||||:||.:
Mouse   509 GWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLE 573

  Fly   487 VGGIQFTATTFSGWYMSTEIGSRNLCDTNRRNMLETVALKMQLDTRTPTSLWKDKAVVEMNIAVL 551
            :||::|:|..||||||.||||.|:.||.:|.|:||.||.||.||.|..:|||||:|:||:|||||
Mouse   574 IGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVL 638

  Fly   552 HSYQSRNVTIVDHHTASESFMKHFENESKLRNGCPADWIWIVPPLSGSITPVFHQEMALYYLKPS 616
            :|:||..|||||||:|:|||:||.|||.:.|.||||||:|||||:||||||||||||..|.|.||
Mouse   639 YSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPS 703

  Fly   617 FEYQDPAWRTHVWKKGRGESKGKKPRRKFNFKQIARAVKFTSKLFGRALSKRIKATVLYATETGK 681
            ||||...|.|||||   |.:.....||...||::|.||||::||.|:|::||:|||:||||||||
Mouse   704 FEYQPDPWNTHVWK---GTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGK 765

  Fly   682 SEQYAKQLCELLGHAFNAQIYCMSDYDISSIEHEALLIVVASTFGNGDPPENGELFSQELYAMR- 745
            |:.|||.|||:..|||:|:...|.:|||..:|||||::||.|||||||||||||.|...|..||
Mouse   766 SQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRH 830

  Fly   746 ---VQES-SEHGLQDSSIGSSKSFMKASSRQEFMKLPLQQVKRIDRWDSLRGSTSDTFTEETFGP 806
               |||. ..:.::.:|:.|.....|:|.               |..| ||    |.|  |:.||
Mouse   831 PNSVQEERKSYKVRFNSVSSYSDSRKSSG---------------DGPD-LR----DNF--ESTGP 873

  Fly   807 LSNVRFAVFALGSSAYPNFCAFGQYVDNILGELGGERLLRVAYGDEMCGQEQSFRKWAPEVFKLA 871
            |:||||:||.|||.|||:|||||..||.:|.||||||:|::..|||:||||::||.||.:|||.|
Mouse   874 LANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAA 938

  Fly   872 CETFCLDPEESLSDASLAL-QND-SLTVNTVRLVPSANKGSLDSSLSKYHNKKVHCCKAKAKPHN 934
            |:.||:..:.::..|:.:| .|| |...|..||...|....|...||..|.|:|...:..:: .|
Mouse   939 CDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSR-QN 1002

  Fly   935 LTRLSEGAKTTMLLEICAPG---LEYEPGDHVGIFPANRTELVDGLLNRLVGVDNPDEVLQLQLL 996
            | :..:.:::|:.:.:...|   |:|:||||:|:||.|..:||:.|:.||......:.|:::::|
Mouse  1003 L-QSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEML 1066

  Fly   997 KEKQTSNGIFKCWEPHDKIPPDTLRNLLARFFDLTTPPSRQLLTLLAGFCEDTADKERLELLVND 1061
            :|:.|:.|:...|:...::||.|:......:.|:||||:...|...|....:..:|:||.:|...
Mouse  1067 EERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKG 1131

  Fly  1062 SSAYEDWRHWRLPHLLDVLEEFPSCRPPAPLLLAQLTPLQPRFYSISSSPRRVSDEIHLTVAIVK 1126
            ...||:|:..:.|.:::|||||||.:.||.|||.||:.||||:|||||||....||:|||||||.
Mouse  1132 LQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVS 1196

  Fly  1127 YRCEDGQGDERYGVCSNYLSGLRADDELFMFVRSALGFHLPSDRSRPIILIGPGTGIAPFRSFWQ 1191
            |...||:|...:||||::|:.::|||.:..|||.|..||||.:...|.||:||||||||||||||
Mouse  1197 YHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQ 1261

  Fly  1192 EFQVLSDLDPTAKLP-KMWLFFGCRNRDVD-LYAEEKAELQKDQILDRVFLALSREQAIPKTYVQ 1254
            :.|.  |:......| .|.|.||||...:| :|.||..:.:...:...::.|.|||...||.|||
Mouse  1262 QRQF--DIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQ 1324

  Fly  1255 DLIEQEF-DSLYQLIVQERGHIYVCGDVTMAEHVYQTIRKCIAGKEQKSEAEVETFLLTLRDESR 1318
            |:::::. :|:|:.:.::.||||||||||||..|.:.|::.:..:.:.||.:...|:..|||::|
Mouse  1325 DVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNR 1389

  Fly  1319 YHEDIFGITLRTAEIHTKSRATARIRMAS 1347
            |||||||:||||.|:      |.|:|..|
Mouse  1390 YHEDIFGVTLRTYEV------TNRLRSES 1412

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nos	NP_523541.2	NOS_oxygenase_euk	216..624	CDD:238410	253/410 (62%)
		CysJ	669..1323	CDD:223446	290/666 (44%)
		Flavodoxin_1	673..863	CDD:278677	100/194 (52%)
		Nitric_oxide_synthase	931..1328	CDD:99799	167/402 (42%)
Nos1	NP_032738.1	Interaction with NOSIP. /evidence=ECO:0000250	1..200		13/60 (22%)
		PDZ_signaling	15..98	CDD:238492
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	114..174		9/26 (35%)
		PIN (nNOS-inhibiting protein) binding. /evidence=ECO:0000250	163..240		16/84 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	271..298		7/26 (27%)
		NOS_oxygenase_euk	300..711	CDD:238410	254/425 (60%)
		Calmodulin-binding	725..745		10/19 (53%)
		Tetrahydrobiopterin-binding. /evidence=ECO:0000250	750..769		14/18 (78%)
		CysJ	754..1394	CDD:223446	290/665 (44%)
		Flavodoxin_1	757..930	CDD:278677	100/194 (52%)
		Nitric_oxide_synthase	996..1399	CDD:99799	167/406 (41%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	526	1.000	Domainoid score	I296
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H37327
Inparanoid	1	1.050	1113	1.000	Inparanoid score	I226
Isobase	1	0.950	-	0.75	Normalized mean entropy	S1636
OMA	1	1.010	-	-		QHG46820
OrthoDB	1	1.010	-	-		D56017at33208
OrthoFinder	1	1.000	-	-		FOG0001418
OrthoInspector	1	1.000	-	-		otm44105
orthoMCL	1	0.900	-	-		OOG6_103176
Panther	1	1.100	-	-	O	PTHR19384
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X3122
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	13	12.930