Protein Alignment Nos and Nos1

DIOPT Version :10

• Sequence 1: NP_523541.2
• Gene: Nos / 34495
• FlyBaseID: FBgn0011676
• Length: 1349
• Species: Drosophila melanogaster
• Sequence 2: NP_001423318.1
• Gene: Nos1 / 18125
• MGIID: 97360
• Length: 1463
• Species: Mus musculus
• Alignment Length: 1345
• Identity: 614/1345 - (45%)
• Similarity: 830/1345 - (61%)
• Gaps: 127/1345 - (9%)

  Fly    78 GGDSSPSHEVDHPGGAQG-AQAAGGLPSSSGT---PLRHHKRASISTASPPIRERRGTNTSIVVE 138
            |..:.|.|       ||| .|.||.:..::|.   |...:.:|::..:        |....::.|
Mouse   154 GPSNGPQH-------AQGRGQGAGSVSQANGVAIDPTMKNTKANLQDS--------GEQDELLKE 203

  Fly   139 LD-------GSGSGSGSGG--------GGVGV-----GQGAGCPPSGSCTASGKSSREL------ 177
            ::       |.|.....||        .|:.|     |:....||.|     |::.|..
Mouse   204 IEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLG-----GENDRVFNDLWGK 263

  Fly   178 --------SPSPKNQQQPRKMSQD-----YRSRAGSFMHLDDEGRSLLMRKPMRLKNIEGRPEVY 229
                    :|..:|:|.|....|.     ..||...|               :::||.|....:.
Mouse   264 GNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRF---------------LKVKNWETDVVLT 313

  Fly   230 DTLHCKGREILSCSKATCTSSIMNIGN---AAVEARKSDLILEHAKDFLEQYFTSIKRTSSTAHE 291
            ||||.|......|::..|..|||...:   .:.:.|..|.:...||:||:||::||||..|.||.
Mouse   314 DTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHM 378

  Fly   292 TRWKQVRQSIETTGHYQLTETELIYGAKLAWRNSSRCIGRIQWSKLQVFDCRYVTTTSGMFEAIC 356
            .|.::|.:.||:|..|||.:||||||||.||||:|||:||||||||||||.|..||..|||..||
Mouse   379 DRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYIC 443

  Fly   357 NHIKYATNKGNLRSAITIFPQRTDAKHDYRIWNNQLISYAGYKQADGKIIGDPMNVEFTEVCTKL 421
            ||:|||||||||||||||||||||.|||:|:||:|||.||||||.||..:|||.||||||:|.:.
Mouse   444 NHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQ 508

  Fly   422 GWKSKGSEWDILPLVVSANGHDPDYFDYPPELILEVPLTHPKFEWFSDLGLRWYALPAVSSMLFD 486
            |||.....:|:|||::.|||:||:.|..||||:||||:.||||:||.||||:||.|||||:||.:
Mouse   509 GWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLE 573

  Fly   487 VGGIQFTATTFSGWYMSTEIGSRNLCDTNRRNMLETVALKMQLDTRTPTSLWKDKAVVEMNIAVL 551
            :||::|:|..||||||.||||.|:.||.:|.|:||.||.||.||.|..:|||||:|:||:|||||
Mouse   574 IGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVL 638

  Fly   552 HSYQSRNVTIVDHHTASESFMKHFENESKLRNGCPADWIWIVPPLSGSITPVFHQEMALYYLKPS 616
            :|:||..|||||||:|:|||:||.|||.:.|.||||||:|||||:||||||||||||..|.|.||
Mouse   639 YSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPS 703

  Fly   617 FEYQDPAWRTHVWKKGRGESKGKKPRRKFNFKQIARAVKFTSKLFGRALSKRIKATVLYATETGK 681
            ||||...|.|||||   |.:.....||...||::|.||||::||.|:|::||:|||:||||||||
Mouse   704 FEYQPDPWNTHVWK---GTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGK 765

  Fly   682 SEQYAKQLCELLGHAFNAQIYCMSDYDISSIEHEALLIVVASTFGNGDPPENGELFSQELYAMR- 745
            |:.|||.|||:..|||:|:...|.:|||..:|||||::||.|||||||||||||.|...|..||
Mouse   766 SQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRH 830

  Fly   746 ---VQESSEH----------GLQDSSIGSSKSFMKAS--SRQEFMKLPLQQVKRIDRWDSLRGS- 794
               |||..::          |...|...|....:.|:  |:....|:....|....  ||.:.|
Mouse   831 PNSVQEERKYPEPLRFFPRKGPSLSHADSEAHSLVAARDSQHRSYKVRFNSVSSYS--DSRKSSG 893

  Fly   795 ----TSDTFTEETFGPLSNVRFAVFALGSSAYPNFCAFGQYVDNILGELGGERLLRVAYGDEMCG 855
                ..|.|  |:.|||:||||:||.|||.|||:|||||..||.:|.||||||:|::..|||:||
Mouse   894 DGPDLRDNF--ESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCG 956

  Fly   856 QEQSFRKWAPEVFKLACETFCLDPEESLSDASLAL-QND-SLTVNTVRLVPSANKGSLDSSLSKY 918
            ||::||.||.:|||.||:.||:..:.::..|:.:| .|| |...|..||...|....|...||..
Mouse   957 QEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNV 1021

  Fly   919 HNKKVHCCKAKAKPHNLTRLSEGAKTTMLLEICAPG---LEYEPGDHVGIFPANRTELVDGLLNR 980
            |.|:|...:..:: .|| :..:.:::|:.:.:...|   |:|:||||:|:||.|..:||:.|:.|
Mouse  1022 HKKRVSAARLLSR-QNL-QSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIER 1084

  Fly   981 LVGVDNPDEVLQLQLLKEKQTSNGIFKCWEPHDKIPPDTLRNLLARFFDLTTPPSRQLLTLLAGF 1045
            |......:.|:::::|:|:.|:.|:...|:...::||.|:......:.|:||||:...|...|..
Mouse  1085 LEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASL 1149

  Fly  1046 CEDTADKERLELLVNDSSAYEDWRHWRLPHLLDVLEEFPSCRPPAPLLLAQLTPLQPRFYSISSS 1110
            ..:..:|:||.:|......||:|:..:.|.:::|||||||.:.||.|||.||:.||||:||||||
Mouse  1150 ATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSS 1214

  Fly  1111 PRRVSDEIHLTVAIVKYRCEDGQGDERYGVCSNYLSGLRADDELFMFVRSALGFHLPSDRSRPII 1175
            |....||:|||||||.|...||:|...:||||::|:.::|||.:..|||.|..||||.:...|.|
Mouse  1215 PDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCI 1279

  Fly  1176 LIGPGTGIAPFRSFWQEFQVLSDLDPTAKLP-KMWLFFGCRNRDVD-LYAEEKAELQKDQILDRV 1238
            |:||||||||||||||:.|.  |:......| .|.|.||||...:| :|.||..:.:...:...:
Mouse  1280 LVGPGTGIAPFRSFWQQRQF--DIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFREL 1342

  Fly  1239 FLALSREQAIPKTYVQDLIEQEF-DSLYQLIVQERGHIYVCGDVTMAEHVYQTIRKCIAGKEQKS 1302
            :.|.|||...||.||||:::::. :|:|:.:.::.||||||||||||..|.:.|::.:..:.:.|
Mouse  1343 YTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLS 1407

  Fly  1303 EAEVETFLLTLRDESRYHEDIFGITLRTAEIHTKSRATARIRMAS 1347
            |.:...|:..|||::||||||||:||||.|:      |.|:|..|
Mouse  1408 EEDAGVFISRLRDDNRYHEDIFGVTLRTYEV------TNRLRSES 1446

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nos	NP_523541.2	NOS_oxygenase_euk	216..624	CDD:238410	253/410 (62%)
		Flavodoxin_1	673..863	CDD:425562	102/210 (49%)
		Nitric_oxide_synthase	931..1328	CDD:99799	167/402 (42%)
Nos1	NP_001423318.1	PDZ_nNOS-like	14..123	CDD:467192
		NOS_oxygenase_euk	300..711	CDD:238410	254/425 (60%)
		Flavodoxin_1	757..964	CDD:425562	102/210 (49%)
		Nitric_oxide_synthase	1030..1433	CDD:99799	167/406 (41%)