Protein Alignment Nos and nos2

DIOPT Version :9

• Sequence 1: NP_523541.2
• Gene: Nos / 34495
• FlyBaseID: FBgn0011676
• Length: 1349
• Species: Drosophila melanogaster
• Sequence 2: XP_002935342.2
• Gene: nos2 / 100494698
• XenbaseID: XB-GENE-485093
• Length: 1160
• Species: Xenopus tropicalis
• Alignment Length: 1139
• Identity: 500/1139 - (43%)
• Similarity: 693/1139 - (60%)
• Gaps: 119/1139 - (10%)

  Fly   215 KPMRLKNIEGRPEVYDTLHCKGREILSCSKATCTSSIMN----IGNAAVEARKSDLILEHAKDFL 275
            |.::|||.|....:.||||.|.::.|||....|..||||    |.....|....|.:|..|.:|:
 Frog    80 KYLKLKNYESGSILQDTLHLKAKKNLSCQSKICQGSIMNPKVYIRGPRNEPVPQDELLPQAIEFV 144

  Fly   276 EQYFTSIKRTSSTAHETRWKQVRQSIETTGHYQLTETELIYGAKLAWRNSSRCIGRIQWSKLQVF 340
            .||::|.|......|..|.:.|.:.||.||.||||..||.|..|:||||:.|||||||||.||||
 Frog   145 NQYYSSFKEPQIENHLNRLETVAKEIECTGTYQLTRDELAYATKMAWRNAPRCIGRIQWSNLQVF 209

  Fly   341 DCRYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQRTDAKHDYRIWNNQLISYAGYKQADGKI 405
            |.|...|...|||.:|.||.|::|.||:||||||||||||.|||:|:||:|||.|||::..||.|
 Frog   210 DARDCKTAKEMFELLCRHITYSSNGGNIRSAITIFPQRTDGKHDFRVWNSQLIRYAGFQMPDGSI 274

  Fly   406 IGDPMNVEFTEVCTKLGWKSKGSEWDILPLVVSANGHDPDYFDYPPELILEVPLTHPKFEWFSDL 470
            :|||.::||||:|..||||.|.|.::|||||:.|||.||:.|:.|.::|.|||:.|||:|||.:|
 Frog   275 VGDPASIEFTELCIHLGWKPKYSRFEILPLVLQANGQDPELFEIPLDIIDEVPIEHPKYEWFKEL 339

  Fly   471 GLRWYALPAVSSMLFDVGGIQFTATTFSGWYMSTEIGSRNLCDTNRRNMLETVALKMQLDTRTPT 535
            .|:||.||||::||.:|||::|:|..|:||||.||||.|:.||..|.|:||.|..::.|:|....
 Frog   340 SLKWYTLPAVANMLLEVGGLEFSACPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRLGLETHKLA 404

  Fly   536 SLWKDKAVVEMNIAVLHSYQSRNVTIVDHHTASESFMKHFENESKLRNGCPADWIWIVPPLSGSI 600
            |||||:||:|:|||||:|:|..||||:|||:|:||||||.|||.:||.||||||:|:|||:||||
 Frog   405 SLWKDQAVIELNIAVLYSFQKHNVTIMDHHSAAESFMKHLENEYRLRGGCPADWVWLVPPMSGSI 469

  Fly   601 TPVFHQEMALYYLKPSFEYQDPAWRTHVWKKGRGESKGKKPRRK-FNFKQIARAVKFTSKLFGRA 664
            ||||||||..|.|.|.:.||..||:||||     :.:.:||::| |.|..:|::|.|.|.|..:.
 Frog   470 TPVFHQEMLNYILSPFYYYQIDAWKTHVW-----QDENRKPKKKEFKFSVVAKSVLFASSLLKKT 529

  Fly   665 LSKRIKATVLYATETGKSEQYAKQLCELLGHAFNAQIYCMSDYDISSIEHEALLIVVASTFGNGD 729
            ::.|.|.|:||||||||||.:||:|..|..:||||::.||.||:|.::|.|:||:||.|||||||
 Frog   530 VAARTKVTILYATETGKSETFAKKLQALFSYAFNAKVLCMDDYNIRNLEDESLLLVVTSTFGNGD 594

  Fly   730 PPENGELFSQELYAMRVQESSEHGLQDSSIGSSKSFMKASSRQEFMKLPLQQVKRIDRWDSLRGS 794
            .|.|||.|.:.:::.:                            ::|
 Frog   595 CPGNGEKFKKAMFSCK----------------------------YLK------------------ 613

  Fly   795 TSDTFTEETFGPLSNVRFAVFALGSSAYPNFCAFGQYVDNILGELGGERLLRVAYGDEMCGQEQS 859
                         :.:|:|||.||||.||.||||...||..|.:||..::.....|||:.|||:|
 Frog   614 -------------NKLRYAVFGLGSSMYPQFCAFAHTVDQRLAQLGAAQISLTGEGDELSGQEES 665

  Fly   860 FRKWAPEVFKLACETF------------CLDPEESLSDASLALQNDSLTVNTVRLVPSANKGSLD 912
            |..||.:.||.|||.|            |....||.|..:..|.||:...:.::
 Frog   666 FLNWAVQTFKAACELFKIRDRRNIHLPKCYTSTESWSAENYRLVNDNQQYDHIK----------- 719

  Fly   913 SSLSKYHNKKVHCCKAKAKPHNLTRLSEGAKTTMLLEICAPGLEYE--------PGDHVGIFPAN 969
             :|.|.|::.:...|.|.: .|| :..:.::||:|::     |.||        ||:|.|:|..|
 Frog   720 -ALCKMHSRSIVPMKLKFR-KNL-QSPKSSRTTLLVK-----LSYENSQEVRFMPGEHAGLFAGN 776

  Fly   970 RTELVDGLLNRLVGVDNPDEVLQLQLLKEKQTSNGIFKCWEPHDKIPPDTLRNLLARFFDLTTPP 1034
            :.|||..:::.|......::.::|:...|:::      .|...:||||.:|...|..|.|:||||
 Frog   777 QPELVASVISHLKDAPPCNQHVRLETRNEQES------FWTISEKIPPCSLTQALTYFLDITTPP 835

  Fly  1035 SRQLLTLLAGFCEDTADKERLELLVNDSSAYEDWRHWRLPHLLDVLEEFPSCRPPAPLLLAQLTP 1099
            ::.||..|:.......||.|||.|..|:..|:.|::...|:.|:|||||||.:.|...:|.||.|
 Frog   836 TQLLLKKLSLLATKVTDKSRLEELSKDADEYKQWKNHNFPNFLEVLEEFPSLQVPTTFVLTQLPP 900

  Fly  1100 LQPRFYSISSSPRRVSDEIHLTVAIVKYRCEDGQGDERYGVCSNYLSGLRADDELFMFVRSALGF 1164
            |:||:||||||...|..|||||||:|.|....|||...:||||.:|:.|..::.:..|:||...|
 Frog   901 LKPRYYSISSSRDMVPGEIHLTVAVVNYTTRGGQGPLHHGVCSTWLNDLDINETVPCFIRSTNTF 965

  Fly  1165 HLPSDRSRPIILIGPGTGIAPFRSFWQEFQVLSDLDPTA-KLPKMWLFFGCRNRDVD-LYAEEKA 1227
            |||.:.|.|.:||||||||:|||.|||  |.|.||:... |...|.|.|||:...:| :||:|..
 Frog   966 HLPENPSVPCVLIGPGTGISPFRGFWQ--QRLYDLEKKGIKAGSMILLFGCQQSGMDEIYADETQ 1028

  Fly  1228 ELQKDQILDRVFLALSREQAIPKTYVQDLIEQEFDS-LYQLIVQERGHIYVCGDVTMAEHVYQTI 1291
            .::...:|..|:.|.||:....|.||||::.:..:| :.:::::|.||:||||:..||::|.||:
 Frog  1029 YMKSKGVLKDVYTAYSRQPGQAKVYVQDILIKNLESEVCRILLEEEGHLYVCGNFEMAKNVAQTL 1093

  Fly  1292 RKCIAGKEQKSEAEVETFLLTLRDESRYHEDIFG 1325
            :..||.:.:.:|.|.|.:...|:::.||||||||
 Frog  1094 KSMIAKRLKLNEEEAENYFSELKNKLRYHEDIFG 1127

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nos	NP_523541.2	NOS_oxygenase_euk	216..624	CDD:238410	230/411 (56%)
		CysJ	669..1323	CDD:223446	248/676 (37%)
		Flavodoxin_1	673..863	CDD:278677	69/189 (37%)
		Nitric_oxide_synthase	931..1328	CDD:99799	161/406 (40%)
nos2	XP_002935342.2	NOS_oxygenase_euk	81..493	CDD:238410	230/411 (56%)
		Flavodoxin_1	538..669	CDD:365983	69/189 (37%)
		FNR_like	733..1127	CDD:384719	161/408 (39%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	514	1.000	Domainoid score	I315
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1094	1.000	Inparanoid score	I241
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D56017at33208
OrthoFinder	1	1.000	-	-		FOG0001418
OrthoInspector	1	1.000	-	-		otm49279
Panther	1	1.100	-	-	O	PTHR19384
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	7	7.160