Protein Alignment Klp31E and Kif4a

DIOPT Version :9

• Sequence 1: NP_001162935.1
• Gene: Klp31E / 34422
• FlyBaseID: FBgn0032243
• Length: 1048
• Species: Drosophila melanogaster
• Sequence 2: XP_006257175.1
• Gene: Kif4a / 84393
• RGDID: 620526
• Length: 1231
• Species: Rattus norvegicus
• Alignment Length: 1149
• Identity: 328/1149 - (28%)
• Similarity: 543/1149 - (47%)
• Gaps: 199/1149 - (17%)

  Fly     1 MISEVDSINKDSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGSDKAFTFDYVFDTNSNQ 65
            |..||..|    .||||:|.||..|:|:.:.|:.|.:...||||:.:|:||:||:|:|||.::.|
  Rat     1 MKEEVKGI----PVRVALRCRPLVSKEINEGCQTCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQ 61

  Fly    66 CDIYSDCVEKLVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFS 130
            .::::..|..|:.....||||||||||||||||||:||..:..|.|...::  |:|||.::.:|.
  Rat    62 EEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQEHDSAI--GVIPRVIQLLFK 124

  Fly   131 GIEQLEGSSTSEHPAAGGSPQFSLAVQYIELYNEDIFDLL-DPFNKNSNFKIHEDASGQITISGA 194
            .|.:....            :|:|.|.|:|:|||:|.||| ....|.|...|.||....|.|.|.
  Rat   125 EINKKTDF------------EFTLKVSYLEIYNEEILDLLCSSREKASQINIREDPKEGIKIVGL 177

  Fly   195 SIKPIYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQRLLTPSDNVPDNDL-ETL 258
            :.|.:....|.:..|:||...||.|||.||.|||||||:|||.:.:::         .||. .:.
  Rat   178 TEKTVLVASDTVSCLEQGNNCRTVASTAMNSQSSRSHAIFTISIEQRK---------KNDKNSSF 233

  Fly   259 TSKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRL 323
            .||.|.||||||||.|:|:|.|:|.:|||:||.|||.|||.|||||| .|:...|||||||||||
  Rat   234 RSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGD-DKKGNFVPYRDSKLTRL 297

  Fly   324 LQDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKINQDQSSRTISQLRREIAAL 388
            |||||||||.|||||||||:|.:..||||||:||:|||.||||..||.|..:..::.|::::..|
  Rat   298 LQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIINIDPQAAELNHLKQQVQQL 362

  Fly   389 QLELLEYKQGKLIVDCEGNTTISDTFN------ENKLLLSEIKRLQQRLKSLQSTIATLTQR--- 444
            |:.||:...|.|    .||..:..:.|      :|:.|:.|.::|.:.|.......|.:.:|
  Rat   363 QVLLLQAHGGTL----PGNINVKPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIIL 423

  Fly   445 --NADLKTNLDLNKWTSDDNSDVEIAKVVGQYMLEIEELQTKLIESEEHRKQMEIKATTSPRNAK 507
              .|:.|.|..|.:........|::.|:            .:.:|.:|.::.:||........|:
  Rat   424 TEQANEKMNAKLEELRQHAACKVDLQKL------------AETLEDQEVKENIEIICNLQQAIAR 476

  Fly   508 PVYDSDIITKAKKDLERERELLMSRSLPGIQNQNVSSEETEVASSDSEAE------EVVKDLEAI 566
            ...::.....|..|...|           :..|..||.:|..:|.....:      ::.|:|..:
  Rat   477 LSDEAVACMTATIDTAGE-----------VDTQEQSSPDTSRSSDVFSTQHALRQAQMSKELIEL 530

  Fly   567 DNDIEMRTKLIEQLELTNSRYEQMRTHYEEKLSVLYCKIENTQKERDDVLANMTTSVSTPSKD-S 630
            :.::.::..|.:::...:::.:.::..|::.:..|..::.:.|||:::::..:.|:    .|| :
  Rat   531 NKELALKEALAKKMTQNDNQLQPIQFQYQDNIKNLELEVLSLQKEKEELVLELQTA----KKDVN 591

  Fly   631 LKKVKTDYESKISHMQTEIRKLQNAQREHIRSKQQLKSHEVRIGTLRNELNELKFAKVKLMKKMS 695
            ..|:......::..::.:|.:|:....|..:..:..::.|:.:..|..|:..:|..:|:||::|.
  Rat   592 QAKLSERRRKRLQELEGQIAELKKKLHEQSKLLKLKETTELTVSKLNQEIRMMKNQRVQLMRQMK 656

  Fly   696 QQSNRHKQEESRKSKEIAQLLKEQRRQKNAVLTLEAKVSAKEQILKRKTEEVIALRK-------- 752
            :.:.:.:|.:.:|.||:.||.:..|:::..:|.||.....:..:|:|||||..|..|
  Rat   657 EDAEKFRQWKQQKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQK 721

  Fly   753 -----------SQRGKSG-------------------QRAPAHLTS-----KIATLDGFSTRSAR 782
                       ..||..|                   :.|..||..     ||...|....:..|
  Rat   722 QKEVAEKRKETQSRGMEGTAARMKSWLGNEIEVMVSTEEAKRHLNDLLEERKILAQDVAQLKEKR 786

  Fly   783 HRWEN---------LYRNILHAARN------RQLITQLEKELERLILEREDLSRELNIMENGQTA 832
            ...||         ..|:.:|...:      .:.|..||.|||....:..||.::|...|:....
  Rat   787 ESGENPPPKLRRRTFSRDEVHGQDSGAEDSISKQIESLESELELRSAQIADLQQKLLDAESEDQP 851

  Fly   833 DRQTDEF--------------NEVDNLKTNISYIQENIEHVQQAIMEFEDTKDTVQSHVNKIQSL 883
            .::.:..              .|:.:.|..:|.::..:...:.:.:|.:......|:|..||::.
  Rat   852 KQRWENIATILEAKCAIKYLVGELVSSKIQVSKLESGLSQSKASCIEVQKMLFEEQNHFAKIETE 916

  Fly   884 LDEVSTVAEAKFILQKFSDTAIVMSCNL---AIAESHLQE-----KESLLKEVK-QESGIQ--QQ 937
            |.|     |...:.|:..:..:.:...|   .:.|..|:|     ::.||..:| ||..::  |:
  Rat   917 LKE-----ELVRVEQQHQEKVLYLLSQLQQCQVTEKQLEESVSEKEQQLLSTLKCQEEELRKMQE 976

  Fly   938 IL---QHFLSQNSNVHIADIFDSLNLKGSNIASSVNPGSQKSLISNATYDIPQDDKNSEFHQVEM 999
            :.   |..|.:||.:.     ..|||  ..:.|...|...::::.:         .:|.|..:
  Rat   977 VCEQNQQLLQENSAIK-----QKLNL--LQVVSKEKPHLVRNILQS---------PDSSFEYI-- 1023

  Fly  1000 RRTTSRSPSPLGNSDPFDKSPKVRRRTAKRQDL-LFGDTELSEQ 1042
                  .|.|    .||....|...::...:|| .:.:|.::|:
  Rat  1024 ------PPKP----KPFRIKEKCPEKSFAIEDLQYYSETSVAEE 1057

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp31E	NP_001162935.1	KISc_KIF4	12..364	CDD:276823	174/353 (49%)
		KISc	13..370	CDD:214526	178/358 (50%)
		KASH_CCD	409..589	CDD:291334	30/196 (15%)
		RasGAP	<685..750	CDD:295371	20/64 (31%)
Kif4a	XP_006257175.1	KISc_KIF4	8..338	CDD:276823	175/357 (49%)
		KISc	9..344	CDD:214526	178/358 (50%)
		HCR	390..1057	CDD:284517	132/726 (18%)
		COG4985	<494..638	CDD:227318	22/158 (14%)
		Cas1_I-II-III	<681..>744	CDD:294435	15/62 (24%)
		Pre-SET	<1070..1120	CDD:282838

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0244
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D74122at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.720