DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Klp31E and kif21a

DIOPT Version :9

Sequence 1:	NP_001162935.1	Gene:	Klp31E / 34422	FlyBaseID:	FBgn0032243	Length:	1048	Species:	Drosophila melanogaster
Sequence 2:	XP_009296478.1	Gene:	kif21a / 566481	ZFINID:	ZDB-GENE-110411-237	Length:	1598	Species:	Danio rerio

Alignment Length:	1190	Identity:	471/1190 - (39%)
Similarity:	664/1190 - (55%)	Gaps:	207/1190 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 DSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGSDKAFTFDYVFDTNSNQCDIYSDCVEK 75
            ||:||||:|||||.::|.|:.|.|||.|...|||:.||.|||||:|||||.:|.|.:|||:|.||
Zfish     3 DSTVRVALRIRPQLAKEKIEGCHICTFVMPDEPQVVLGKDKAFTYDYVFDMDSTQDNIYSNCTEK 67

  Fly    76 LVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFSGIEQ-LEGSS 139
            |::....|||||:.||||||||||||||||||......|   ||||||||.|:|.|||| .:.::
Zfish    68 LIEGCFEGYNATIFAYGQTGSGKTYTMGTGFDVAIPDED---LGIIPRAVTHLFKGIEQRRQQAA 129

  Fly   140 TSEHPAAGGSPQFSLAVQYIELYNEDIFDLLDPF------NKNSNFKIHEDASGQITISGASIKP 198
            ....|.    |:|.::.|::|||||::.||.|..      .:.|:.||||||||.|...|.:.:.
Zfish   130 EQSRPV----PEFKISAQFLELYNEEVLDLFDTTRDMESRKQKSHIKIHEDASGGIYTVGVTTRN 190

  Fly   199 IYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQRLLTPSDNVPDND--------- 254
            :....:.::.|:.|||:||||||:||.|||||||:|||.:.:.|:..|:|:.|:.|
Zfish   191 VSSEAEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHICQIRVCAPADSPPEQDNETDNRLAG 255

  Fly   255 -------LETLTSKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALH 312
                   .||||:||||||||||||||||.||||||:||||||||||||||.||||||:|||:.|
Zfish   256 SSSEMEEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDRSKRSTH 320

  Fly   313 VPYRDSKLTRLLQDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKINQDQSSRT 377
            ||||||||||||||||||||:|:||||:||||:||||||||||||||||||||||.:|||::|:.
Zfish   321 VPYRDSKLTRLLQDSLGGNSRTVMIACISPSDQDFMETLNTLKYANRARNIKNKVMVNQDRASQQ 385

  Fly   378 ISQLRREIAALQLELLEYKQGKLIVDCEGNTTISDTFNENKLLLSEIKRLQQRLKSLQSTI---- 438
            ||.||.|||.||:||:||:.||.:|..:|..:|:|.|:||.:|..|...|:.|:|::|..|
Zfish   386 ISALRTEIARLQMELMEYRTGKRMVGEDGLESINDMFHENSMLQVENNNLRMRVKAMQEAIDAQG 450

  Fly   439 ATLTQRNADLKTNLDLNKWTSDDNSDVEIAKVVGQYMLEIEELQTKLIESE---EHRKQMEIKAT 500
            |.|||..:.....|...   :.:.|: ||..::..|:.|||:|:.||:|||   |:.::...:|:
Zfish   451 ARLTQLLSQQANQLLAR---AGEGSE-EIGNMIQNYIKEIEDLRAKLLESEAVNENLRRNLTRAS 511

  Fly   501 TSPR-------------NAKPVYD-SDIITKAKKDLE--RERELLMSRSLPGI------------ 537
            :.|:             ...|..: ||||..||||||  :.||....:.|..:
Zfish   512 SRPQFYGASGSFSTALLGPDPSQETSDIIEIAKKDLEKLKRREKKKKKRLQQLLADKRDEDEDEE 576

  Fly   538 -----------------------------------------------QNQNVSSEETEVASSDSE 555
                                                           ..::|..||.|...|..|
Zfish   577 EEVEEDSANKDETQEDGEQKTEKEQSESTNQEPEMEASDREEGDGEEDEEDVEEEEMEAEESSEE 641

  Fly   556 A-------EEVVKDLEAIDNDIEMRTKLIEQLELTNSRYEQMRTHYEEKLSVLYCKIENTQKERD 613
            .       |:...||..|..:|.::.|||::||.:..|...::..||.||.:|..||.:||.|||
Zfish   642 TDSELDEKEDFQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEHKLMMLQSKIRDTQLERD 706

  Fly   614 DVLANMTTSVSTPSKDSLKKVKTDYESKISHMQTEIRKLQNAQREHIRSKQQLKSHEVRIGTLRN 678
            .||.:| .||.:.|:|..|::|.:||.|:|.|..|::|||.||:||.|..:....:|.::..|:.
Zfish   707 RVLHSM-GSVESCSEDKTKRIKAEYEKKLSVMNKELQKLQAAQKEHARLLKNQSQYEKQLKKLQL 770

  Fly   679 ELNELKFAKVKLMKKMSQQSNRHKQEESRKSKEIAQLLKEQRRQKNAVLTLEAKVSAKEQILKRK 743
            :|.|:|..||:|||:|.:|..:.:..|||:::|||.|.|:||:|::.:..|||:...:|.||:||
Zfish   771 DLTEMKKTKVRLMKQMKEQQEKSRMAESRRNREIATLKKDQRKQEHQLKLLEAQKRQQELILRRK 835

  Fly   744 TEEVIALRKSQRGKSGQ----------------RAPAHLTSKIATLDG-----------FSTRSA 781
            ||||.|||:..|..||:                |.||..|......:|           :|||.|
Zfish   836 TEEVTALRRQVRPISGKVTRKANLSEPNFDLSHRPPAGRTYGSGAPNGTRLYHRRAAGIYSTRVA 900

  Fly   782 RHRWENLYRNILHAARNRQLITQLEKELERLILEREDLSRELN--------IMENGQTADRQTDE 838
            |.:|::|.|.|......|..|:.:|.::.||:.:||:|:|..:        ::.:....::....
Zfish   901 RGKWQSLERRISDIIMQRMTISNMEADMNRLLKQREELTRRRDKISRKRERLLADDPEVEKHLQP 965

  Fly   839 FN-EVDNLKTNISYIQENIEHVQQAIMEFEDTKDTVQSHVNKIQSLLDEVSTVAEAKFILQKFSD 902
            .| |:::|..||.||.::|...|..||:.|:.|:  :.....:.:::.. .|:|||:|:|..|..
Zfish   966 VNEELESLLANIDYINDSISDCQANIMQMEEAKE--EGDAVDVSAVISS-CTLAEARFLLDHFMS 1027

  Fly   903 TAIVMSCNLAIAESHLQEKESLLKEVKQESGIQQQILQHFLSQNSNVHIADIFDSLNLKGSNIAS 967
            .|:......|..||.::..|..||:.:..|..|.|:|.|.|.:.                    :
Zfish  1028 MALNKGLQAAQKESQIKVMEGRLKQTEINSATQNQLLFHMLKEK--------------------A 1072

  Fly   968 SVNPGSQKSLISNATYDIPQ------DDKNSEFHQVEMRRTTSRSPSPLGNSDPFD--------K 1018
            ..|| ...:|:.||..::..      ||.:|:        .::.||:..||:...|        |
Zfish  1073 EFNP-ELDALLGNALQELGNVPLENGDDSSSD--------ESTPSPAVEGNTLASDLMKLCGETK 1128

  Fly  1019 S-PKVRRRTAKRQDLLFGDT 1037
            | .|.||||..:.:||:.|:
Zfish  1129 SRTKARRRTTTQMELLYVDS 1148

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Klp31E	NP_001162935.1	KISc_KIF4	12..364	CDD:276823	225/374 (60%)
		KISc	13..370	CDD:214526	229/379 (60%)
		KASH_CCD	409..589	CDD:291334	64/268 (24%)
		RasGAP	<685..750	CDD:295371	30/64 (47%)
kif21a	XP_009296478.1	KISc_KIF4	4..372	CDD:276823	225/374 (60%)
		KISc	6..378	CDD:214526	229/378 (61%)
		SCP-1	<389..809	CDD:114219	133/424 (31%)
		Prefoldin	652..747	CDD:298833	40/95 (42%)
		CEP63	668..973	CDD:293650	107/305 (35%)
		DUF4200	740..834	CDD:290574	38/93 (41%)
		Laminin_I	905..>1091	CDD:283627	50/209 (24%)
		WD40	1266..1577	CDD:238121
		WD40	1272..>1576	CDD:225201
		WD40 repeat	1278..1312	CDD:293791
		WD40 repeat	1317..1351	CDD:293791
		WD40 repeat	1371..1414	CDD:293791
		WD40 repeat	1420..1464	CDD:293791
		WD40 repeat	1471..1505	CDD:293791
		WD40 repeat	1512..1536	CDD:293791
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	420	1.000	Domainoid score	I619
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H56761
Inparanoid	1	1.050	678	1.000	Inparanoid score	I695
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D390391at2759
OrthoFinder	1	1.000	-	-		FOG0002395
OrthoInspector	1	1.000	-	-		otm24916
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	1	1.100	-	-	LDO	PTHR24115
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2095
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.970