DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Klp31E and KIF4B

DIOPT Version :9

Sequence 1:	NP_001162935.1	Gene:	Klp31E / 34422	FlyBaseID:	FBgn0032243	Length:	1048	Species:	Drosophila melanogaster
Sequence 2:	NP_001092763.1	Gene:	KIF4B / 285643	HGNCID:	6322	Length:	1234	Species:	Homo sapiens

Alignment Length:	1113	Identity:	332/1113 - (29%)
Similarity:	554/1113 - (49%)	Gaps:	173/1113 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MISEVDSINKDSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGSDKAFTFDYVFDTNSNQ 65
            |..||..|    .||||:|.||...:|:.:.|::|.:...||.|:.:|:||:||:|:|||..:.|
Human     1 MKEEVKGI----PVRVALRCRPLVPKEISEGCQMCLSFVPGETQVVVGTDKSFTYDFVFDPCTEQ 61

  Fly    66 CDIYSDCVEKLVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFS 130
            .::::..|..|:.....||||||||||||||||||:||..:..|.|:..:|  |||||.::.:|.
Human    62 EEVFNKAVAPLIKGIFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTV--GIIPRVIQLLFK 124

  Fly   131 GIEQLEGSSTSEHPAAGGSPQFSLAVQYIELYNEDIFDLLDPFNKNSNFKIHEDASGQITISGAS 195
            .|::....            :|:|.|.|:|:|||:|.|||.|..:.:...|.||....|.|.|.:
Human   125 EIDKKSDF------------EFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLT 177

  Fly   196 IKPIYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQRLLTPSD-NVPDNDLETLT 259
            .|.:....|.:..|:||..:||.|||.||.|||||||:|||.:.:::   .|| |.      :..
Human   178 EKTVLVALDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISIEQRK---KSDKNC------SFR 233

  Fly   260 SKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRLL 324
            ||.|.||||||||.|:|:|.|:|.:|||:||.|||.|||.|||||| .|:...||||||||||||
Human   234 SKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGD-DKKGSFVPYRDSKLTRLL 297

  Fly   325 QDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKINQDQSSRTISQLRREIAALQ 389
            ||||||||.|||||||||:|.:..|||:||:||:|||.||||..:|.|..:..::.|::::..||
Human   298 QDSLGGNSHTLMIACVSPADSNLEETLSTLRYADRARKIKNKPIVNIDPHTAELNHLKQQVQQLQ 362

  Fly   390 LELLEYKQGKL--IVDCEGNTTISDTFNENKLLLSEIKRLQQRLKSLQSTIATLTQR-------- 444
            :.||:...|.|  .::.|.:..:.....:|:.|:.|.::|.:.|.......|.:.:|
Human   363 VLLLQAHGGTLPGSINAEPSENLQSLMEKNQSLVEENEKLSRCLSKAAGQTAQMLERIILTEQVN 427

  Fly   445 ---NADLK-------TNLDLNKWTSDDNSDVEIAKVVGQYMLEIEELQTKLIESEEHRKQMEIKA 499
               ||.|:       ..|||.|.. :...|.|:.:.| :.:..:::|.|:|.:        |..|
Human   428 EKLNAKLEELRQHVACKLDLQKLV-ETLEDQELKENV-EIICNLQQLITQLSD--------ETVA 482

  Fly   500 TTSPRNAKPVYDSDIITKAKKDLERERELLMSRSLPGIQNQNVSSEETEVASSDSEAEEVVKDLE 564
            .|:         :.|.|..:::.:.|.....|||......|:...:           .::.|::.
Human   483 CTA---------AAIDTAVEEEAQVETSPETSRSSDAFTTQHALHQ-----------AQMSKEVV 527

  Fly   565 AIDNDIEMRTKLIEQLELTNSRYEQMRTHYEEKLSVLYCKIENTQKERDDVLANMTTSVSTPSKD 629
            .::|.:.::..|:.::...:::.:.::..|::.:..|..::.|.|||:::::..:.|:....::.
Human   528 ELNNALALKEALVRKMTQNDNQLQPIQFQYQDNIKNLELEVINLQKEKEELVRELQTAKKNVNQA 592

  Fly   630 SLK----KVKTDYESKISHMQTEIRKLQNAQREHIRSKQQLKSHEVRIGTLRNELNELKFAKVKL 690
            .|.    |:..:.|.:|:    :::|..|.|.:.::.|:   |.|..:..|..|:..:|..:|:|
Human   593 KLSEHRHKLLQELEGQIA----DLKKKLNEQSKLLKLKE---STERTVSKLNQEIWMMKNQRVQL 650

  Fly   691 MKKMSQQSNRHKQEESRKSKEIAQLLKEQRRQKNAVLTLEAKVSAKEQILKRKTEEVIALRK--- 752
            |::|.:.:.:.:|.:.:|.||:.||.:..|:::..:|.||.....:..:|:|||||..|..|
Human   651 MRQMKEDAEKFRQWKQKKDKEVIQLKERDRKRQYELLKLERNFQKQSSVLRRKTEEAAAANKRLK 715

  Fly   753 ----SQR------------GKSG--QRAPAHLTSKIATLDGFSTRSARHRWENLY--RNILHAAR 797
                .||            ||.|  .|....|.::|..:  .||..|:....:|.  |.||
Human   716 DALQKQREVTDKRKETQSHGKEGIAARVRNWLGNEIEVM--VSTEEAKRHLNDLLEDRKIL---- 774

  Fly   798 NRQLITQLEKELERLILEREDLSREL-----NIME-NGQTADRQTDEFNEVDNLKTNISYIQENI 856
             .|.:.||:::.|    .||:...:|     ::.| :||..:.:.....::::|:|.:......|
Human   775 -AQDVVQLKEKKE----SRENPPPKLRKCTFSLSEVHGQVLESEDCITKQIESLETEMELRSAQI 834

  Fly   857 EHVQQAIMEFEDTKDTVQSHVNKIQSLLDEVSTVAEAKFILQKFSDTAIVMSCNLAIAESHLQEK 921
            ..:||.:::.|..        ::.:...:.::|:.|||..|:       .:...|..::.|:.:.
Human   835 ADLQQKLLDAESE--------DRPKQCWENIATILEAKCALK-------YLIGELVSSKIHVTKL 884

  Fly   922 ESLLKEVKQESGIQQQILQHFLSQNSNVHIADIFDSLNL-----------KGSNIASSVNPGSQK 975
            |:.|::.|......|::|  |..||   |.::|...|..           |...:.|.:    |:
Human   885 ENSLRQSKASCADMQKML--FEEQN---HFSEIETELQAELVRMEQQHQEKVLYLVSQL----QE 940

  Fly   976 SLISNATYDIPQDDKNSEF--------HQVEMRRTTSRSPSPLGNSDPFDKSPKVRRRTAKRQDL 1032
            |.::....:....:|..:.        .::|..|........|...:...|...:..:.|.||..
Human   941 SQMAEKQLEKSASEKEQQLVSTLQCQDEELEKMREVCEQNQQLLQENEIIKQKLILLQVASRQKH 1005

  Fly  1033 LFGDTELS 1040
            |..||.||
Human  1006 LPNDTLLS 1013

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Klp31E	NP_001162935.1	KISc_KIF4	12..364	CDD:276823	173/352 (49%)
		KISc	13..370	CDD:214526	177/357 (50%)
		KASH_CCD	409..589	CDD:291334	33/197 (17%)
		RasGAP	<685..750	CDD:295371	20/64 (31%)
KIF4B	NP_001092763.1	KISc_KIF4	8..337	CDD:276823	174/356 (49%)
		KISc	9..343	CDD:214526	177/357 (50%)
		SbcC	406..995	CDD:223496	129/660 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	494..513		4/18 (22%)
		Interaction with PRC1. /evidence=ECO:0000250\|UniProtKB:O95239	663..1234		84/386 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	712..737		4/24 (17%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:O95239	793..798		1/4 (25%)
		Globular. /evidence=ECO:0000250	1000..1234		8/14 (57%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1007..1030		4/7 (57%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1052..1076
		Pre-SET	<1072..1122	CDD:282838
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000250\|UniProtKB:O95239	1086..1144
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1122..1143
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1183..1234
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0244
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D74122at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	4	3.720