Protein Alignment Klp31E and KIF4A

DIOPT Version :10

• Sequence 1: NP_609398.2
• Gene: Klp31E / 34422
• FlyBaseID: FBgn0032243
• Length: 1048
• Species: Drosophila melanogaster
• Sequence 2: NP_036442.3
• Gene: KIF4A / 24137
• HGNCID: 13339
• Length: 1232
• Species: Homo sapiens
• Alignment Length: 1108
• Identity: 327/1108 - (29%)
• Similarity: 539/1108 - (48%)
• Gaps: 163/1108 - (14%)

  Fly     1 MISEVDSINKDSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGSDKAFTFDYVFDTNSNQ 65
            |..||..|    .||||:|.||...:|:.:.|::|.:...||||:.:|:||:||:|:|||.::.|
Human     1 MKEEVKGI----PVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQ 61

  Fly    66 CDIYSDCVEKLVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFS 130
            .::::..|..|:.....||||||||||||||||||:||..:..|.|:..:|  |:|||.::.:|.
Human    62 EEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTV--GVIPRVIQLLFK 124

  Fly   131 GIEQLEGSSTSEHPAAGGSPQFSLAVQYIELYNEDIFDLLDPFNKNSNFKIHEDASGQITISGAS 195
            .|::....            :|:|.|.|:|:|||:|.|||.|..:.:...|.||....|.|.|.:
Human   125 EIDKKSDF------------EFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLT 177

  Fly   196 IKPIYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQRLLTPSDNVPDNDLETLTS 260
            .|.:....|.:..|:||..:||.|||.||.|||||||:|||.:.:::   .||.     ..:..|
Human   178 EKTVLVALDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISLEQRK---KSDK-----NSSFRS 234

  Fly   261 KFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRLLQ 325
            |.|.||||||||.|:|:|.|:|.:|||:||.|||.|||.|||||| .|:...|||||||||||||
Human   235 KLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGD-DKKGGFVPYRDSKLTRLLQ 298

  Fly   326 DSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKINQDQSSRTISQLRREIAALQL 390
            |||||||.|||||||||:|.:..||||||:||:|||.||||..:|.|..:..::.|::::..||:
Human   299 DSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVNIDPQTAELNHLKQQVQQLQV 363

  Fly   391 ELLEYKQGKL--IVDCEGNTTISDTFNENKLLLSEIKRLQQRLKSLQSTIATLTQR-----NADL 448
            .||:...|.|  .:..|.:..:.....:|:.|:.|.::|.:.|.......|.:.:|     .|:.
Human   364 LLLQAHGGTLPGSITVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANE 428

  Fly   449 KTNLDLNKWTSDDNSDVEIAKVVGQYMLEIEELQTKLIESEEHRKQMEIKATTSPRNAKPVYDSD 513
            |.|..|.:........:::.|:|            :.:|.:|.::.:||....          ..
Human   429 KMNAKLEELRQHAACKLDLQKLV------------ETLEDQELKENVEIICNL----------QQ 471

  Fly   514 IITKAKKDLERERELLMSRSLPGIQNQNVSSEETEVASSDSEA---------EEVVKDLEAIDND 569
            :||:    |..|....|:.::.....|....|.:...|..|:|         .::.|:|..::..
Human   472 LITQ----LSDETVACMAAAIDTAVEQEAQVETSPETSRSSDAFTTQHALRQAQMSKELVELNKA 532

  Fly   570 IEMRTKLIEQLELTNSRYEQMRTHYEEKLSVLYCKIENTQKERDDVLANMTTSVSTPSKDSLKKV 634
            :.::..|..::...:|:.:.::..|::.:..|..::.|.|||:::::..:.|:....::..|.:.
Human   533 LALKEALARKMTQNDSQLQPIQYQYQDNIKELELEVINLQKEKEELVLELQTAKKDANQAKLSER 597

  Fly   635 KTDYESKISHMQTEIRKLQNAQREHIRSKQQLKSHEVRIGTLRNELNELKFAKVKLMKKMSQQSN 699
            :.....::.....:::|..|.|.:.::.|:   |.|..:..|..|:..:|..:|:||::|.:.:.
Human   598 RRKRLQELEGQIADLKKKLNEQSKLLKLKE---STERTVSKLNQEIRMMKNQRVQLMRQMKEDAE 659

  Fly   700 RHKQEESRKSKEIAQLLKEQRRQKNAVLTLEAKVSAKEQILKRKTEEVIALRK------------ 752
            :.:|.:.:|.||:.||.:..|:::..:|.||.....:..:|:|||||..|..|
Human   660 KFRQWKQKKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQREV 724

  Fly   753 -------SQRGKSG--QRAPAHLTSKIATLDGFSTRSARHRWENLY--RNILHAARNRQLITQLE 806
                   ..||..|  .|....|.::|..:  .||..|:....:|.  |.||     .|.:.||:
Human   725 ADKRKETQSRGMEGTAARVKNWLGNEIEVM--VSTEEAKRHLNDLLEDRKIL-----AQDVAQLK 782

  Fly   807 KELER-----LILEREDLSRELNIME-NGQTADRQTDEFNEVDNLKTNISYIQENIEHVQQAIME 865
            ::.|.     ..|.|    |..::.| .||.::.:.....::::|:|.:.:....|..:||.:::
Human   783 EKKESGENPPPKLRR----RTFSLTEVRGQVSESEDSITKQIESLETEMEFRSAQIADLQQKLLD 843

  Fly   866 FEDTKDTVQSHVNKIQSLLDEVSTVAEAKFILQKFSDTAIVMSCNLAIAESHLQEKES------- 923
            .|......|...|        ::|:.|||..|:......:.....::..||.|::.::
Human   844 AESEDRPKQRWEN--------IATILEAKCALKYLIGELVSSKIQVSKLESSLKQSKTSCADMQK 900

  Fly   924 -LLKE---------------VKQESGIQQQILQHFLSQNSNVHIADIFDSLNLKGSNIASSVNPG 972
             |.:|               |:.|...|:::| :.|||.....:|:         ..:..||:..
Human   901 MLFEERNHFAEIETELQAELVRMEQQHQEKVL-YLLSQLQQSQMAE---------KQLEESVSEK 955

  Fly   973 SQKSLISNATYDIPQDDKNSEFHQVEMRRTTSRSPSPLGNSDPFDKSPKVRRRTAKRQDLLFGDT 1037
            .|:.|.:....|          .::|..|........|...:...|......:.|.||..|..||
Human   956 EQQLLSTLKCQD----------EELEKMREVCEQNQQLLRENEIIKQKLTLLQVASRQKHLPKDT 1010

  Fly  1038 ELS 1040
            .||
Human  1011 LLS 1013

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp31E	NP_609398.2	KISc_KIF4	12..364	CDD:276823	173/351 (49%)
		Mplasa_alph_rch	<372..>748	CDD:275316	77/391 (20%)
		SMC_prok_B	622..>899	CDD:274008	68/305 (22%)
		Rcc_KIF21	798..870	CDD:410202	16/77 (21%)
KIF4A	NP_036442.3	KISc_KIF4	8..337	CDD:276823	174/355 (49%)
		PRK11281	383..>646	CDD:481316	48/291 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	496..515		4/18 (22%)
		Smc	<517..>1033	CDD:440809	109/539 (20%)
		Required for the interaction with PRC1. /evidence=ECO:0000269|PubMed:15297875	663..1232		84/390 (22%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:11736643	793..798		2/8 (25%)
		Globular	1000..1232		8/14 (57%)
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000269|PubMed:29848660	1086..1144
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1122..1142