DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Klp31E and KIF4A

DIOPT Version :9

Sequence 1:	NP_001162935.1	Gene:	Klp31E / 34422	FlyBaseID:	FBgn0032243	Length:	1048	Species:	Drosophila melanogaster
Sequence 2:	NP_036442.3	Gene:	KIF4A / 24137	HGNCID:	13339	Length:	1232	Species:	Homo sapiens

Alignment Length:	1108	Identity:	327/1108 - (29%)
Similarity:	539/1108 - (48%)	Gaps:	163/1108 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MISEVDSINKDSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGSDKAFTFDYVFDTNSNQ 65
            |..||..|    .||||:|.||...:|:.:.|::|.:...||||:.:|:||:||:|:|||.::.|
Human     1 MKEEVKGI----PVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQ 61

  Fly    66 CDIYSDCVEKLVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFS 130
            .::::..|..|:.....||||||||||||||||||:||..:..|.|:..:|  |:|||.::.:|.
Human    62 EEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTV--GVIPRVIQLLFK 124

  Fly   131 GIEQLEGSSTSEHPAAGGSPQFSLAVQYIELYNEDIFDLLDPFNKNSNFKIHEDASGQITISGAS 195
            .|::....            :|:|.|.|:|:|||:|.|||.|..:.:...|.||....|.|.|.:
Human   125 EIDKKSDF------------EFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLT 177

  Fly   196 IKPIYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQRLLTPSDNVPDNDLETLTS 260
            .|.:....|.:..|:||..:||.|||.||.|||||||:|||.:.:::   .||.     ..:..|
Human   178 EKTVLVALDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISLEQRK---KSDK-----NSSFRS 234

  Fly   261 KFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRLLQ 325
            |.|.||||||||.|:|:|.|:|.:|||:||.|||.|||.|||||| .|:...|||||||||||||
Human   235 KLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGD-DKKGGFVPYRDSKLTRLLQ 298

  Fly   326 DSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKINQDQSSRTISQLRREIAALQL 390
            |||||||.|||||||||:|.:..||||||:||:|||.||||..:|.|..:..::.|::::..||:
Human   299 DSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVNIDPQTAELNHLKQQVQQLQV 363

  Fly   391 ELLEYKQGKL--IVDCEGNTTISDTFNENKLLLSEIKRLQQRLKSLQSTIATLTQR-----NADL 448
            .||:...|.|  .:..|.:..:.....:|:.|:.|.::|.:.|.......|.:.:|     .|:.
Human   364 LLLQAHGGTLPGSITVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANE 428

  Fly   449 KTNLDLNKWTSDDNSDVEIAKVVGQYMLEIEELQTKLIESEEHRKQMEIKATTSPRNAKPVYDSD 513
            |.|..|.:........:::.|:|            :.:|.:|.::.:||....          ..
Human   429 KMNAKLEELRQHAACKLDLQKLV------------ETLEDQELKENVEIICNL----------QQ 471

  Fly   514 IITKAKKDLERERELLMSRSLPGIQNQNVSSEETEVASSDSEA---------EEVVKDLEAIDND 569
            :||:    |..|....|:.::.....|....|.:...|..|:|         .::.|:|..::..
Human   472 LITQ----LSDETVACMAAAIDTAVEQEAQVETSPETSRSSDAFTTQHALRQAQMSKELVELNKA 532

  Fly   570 IEMRTKLIEQLELTNSRYEQMRTHYEEKLSVLYCKIENTQKERDDVLANMTTSVSTPSKDSLKKV 634
            :.::..|..::...:|:.:.::..|::.:..|..::.|.|||:::::..:.|:....::..|.:.
Human   533 LALKEALARKMTQNDSQLQPIQYQYQDNIKELELEVINLQKEKEELVLELQTAKKDANQAKLSER 597

  Fly   635 KTDYESKISHMQTEIRKLQNAQREHIRSKQQLKSHEVRIGTLRNELNELKFAKVKLMKKMSQQSN 699
            :.....::.....:::|..|.|.:.::.|:   |.|..:..|..|:..:|..:|:||::|.:.:.
Human   598 RRKRLQELEGQIADLKKKLNEQSKLLKLKE---STERTVSKLNQEIRMMKNQRVQLMRQMKEDAE 659

  Fly   700 RHKQEESRKSKEIAQLLKEQRRQKNAVLTLEAKVSAKEQILKRKTEEVIALRK------------ 752
            :.:|.:.:|.||:.||.:..|:::..:|.||.....:..:|:|||||..|..|
Human   660 KFRQWKQKKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQREV 724

  Fly   753 -------SQRGKSG--QRAPAHLTSKIATLDGFSTRSARHRWENLY--RNILHAARNRQLITQLE 806
                   ..||..|  .|....|.::|..:  .||..|:....:|.  |.||     .|.:.||:
Human   725 ADKRKETQSRGMEGTAARVKNWLGNEIEVM--VSTEEAKRHLNDLLEDRKIL-----AQDVAQLK 782

  Fly   807 KELER-----LILEREDLSRELNIME-NGQTADRQTDEFNEVDNLKTNISYIQENIEHVQQAIME 865
            ::.|.     ..|.|    |..::.| .||.::.:.....::::|:|.:.:....|..:||.:::
Human   783 EKKESGENPPPKLRR----RTFSLTEVRGQVSESEDSITKQIESLETEMEFRSAQIADLQQKLLD 843

  Fly   866 FEDTKDTVQSHVNKIQSLLDEVSTVAEAKFILQKFSDTAIVMSCNLAIAESHLQEKES------- 923
            .|......|...|        ::|:.|||..|:......:.....::..||.|::.::
Human   844 AESEDRPKQRWEN--------IATILEAKCALKYLIGELVSSKIQVSKLESSLKQSKTSCADMQK 900

  Fly   924 -LLKE---------------VKQESGIQQQILQHFLSQNSNVHIADIFDSLNLKGSNIASSVNPG 972
             |.:|               |:.|...|:::| :.|||.....:|:         ..:..||:..
Human   901 MLFEERNHFAEIETELQAELVRMEQQHQEKVL-YLLSQLQQSQMAE---------KQLEESVSEK 955

  Fly   973 SQKSLISNATYDIPQDDKNSEFHQVEMRRTTSRSPSPLGNSDPFDKSPKVRRRTAKRQDLLFGDT 1037
            .|:.|.:....|          .::|..|........|...:...|......:.|.||..|..||
Human   956 EQQLLSTLKCQD----------EELEKMREVCEQNQQLLRENEIIKQKLTLLQVASRQKHLPKDT 1010

  Fly  1038 ELS 1040
            .||
Human  1011 LLS 1013

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Klp31E	NP_001162935.1	KISc_KIF4	12..364	CDD:276823	173/351 (49%)
		KISc	13..370	CDD:214526	177/356 (50%)
		KASH_CCD	409..589	CDD:291334	31/193 (16%)
		RasGAP	<685..750	CDD:295371	20/64 (31%)
KIF4A	NP_036442.3	KISc_KIF4	8..337	CDD:276823	174/355 (49%)
		SMC_prok_B	<375..714	CDD:274008	71/367 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	496..515		4/18 (22%)
		Smc	558..>918	CDD:224117	79/381 (21%)
		Required for the interaction with PRC1. /evidence=ECO:0000269\|PubMed:15297875	663..1232		84/390 (22%)
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:11736643	793..798		2/8 (25%)
		Globular	1000..1232		8/14 (57%)
		Pre-SET	<1078..1120	CDD:368254
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000269\|PubMed:29848660	1086..1144
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1122..1142
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0244
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D74122at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	4	3.720