Protein Alignment Klp31E and Cenpe

DIOPT Version :10

• Sequence 1: NP_609398.2
• Gene: Klp31E / 34422
• FlyBaseID: FBgn0032243
• Length: 1048
• Species: Drosophila melanogaster
• Sequence 2: NP_776123.3
• Gene: Cenpe / 229841
• MGIID: 1098230
• Length: 2471
• Species: Mus musculus
• Alignment Length: 1162
• Identity: 296/1162 - (25%)
• Similarity: 495/1162 - (42%)
• Gaps: 257/1162 - (22%)

  Fly    10 KDSSVRVAVRIRPQNSRELIDMCRICTTVTLGE-PQIFLGSD----------KAFTFDYVFDTNS 63
            :::||.|.||:||.||||          ..||| ..|:..:|          |:|.||.|||:|.
Mouse     3 EEASVAVCVRVRPLNSRE----------EELGEATHIYWKTDKNAIYQSDGGKSFQFDRVFDSNE 57

  Fly    64 NQCDIYSDCVEKLVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHI 128
            ...::|.:....::.|.:.|||.|:.|||||.||||:||       ..|.|.  ||:||||:..|
Mouse    58 TTKNVYEEIAVPIISSAIQGYNGTIFAYGQTASGKTHTM-------MGSEDC--LGVIPRAIHDI 113

  Fly   129 FSGIEQLEGSSTSEHPAAGGSPQFSLAVQYIELYNEDIFDLLDPFNKNSNFKIHEDASGQITISG 193
            |..|::..            ..:|.|.|.|:|:|||.|.|||....|.....|.||.:..:.:|.
Mouse   114 FQRIKKFP------------EREFLLRVSYMEIYNETITDLLCNAQKMKPLIIREDTNRTVYVSD 166

  Fly   194 ASIKPIYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQRLLTPSDNVPDNDLETL 258
            .:.:.:|....|||:|..|...|....||||.:|||||.:|.:.:..:....||    :.|....
Mouse   167 LTEEVVYTAEMALKWLATGEKNRHYGITKMNQRSSRSHTIFRMILESREKAEPS----NCDGSVK 227

  Fly   259 TSKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRL 323
            .|..:.||||||||..:|.|.|.|.:||..||..|..||..|..|.|...... :.||||||||:
Mouse   228 VSHLNLVDLAGSERAAQTGAEGVRLKEGCFINRNLFILGQVIKKLSDGQVGGF-INYRDSKLTRI 291

  Fly   324 LQDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKINQ-DQSSRTISQLRREIAA 387
            ||:|||||::|.:|..::|:..|  |||.||::|:.|:.:||...:|: ......:.:.|||||.
Mouse   292 LQNSLGGNAKTRIICTITPASLD--ETLTTLQFASTAKYMKNTPYVNEVSNDEALLKRYRREIAD 354

  Fly   388 LQLELLEYKQGKLIVDCEGNTTISDTFNENKLL-------LSEIKR---------LQQRLK---- 432
            |:.:|.|........:.| ...::...:|..||       ::.:||         |||.|:
Mouse   355 LRKQLEEVNTKTRAQEME-KDQLAQLLDEKDLLQKVQDEKINNLKRMLVTSSSIALQQELEIKRK 418

  Fly   433 -------------------SLQSTIATLTQRNA--DLKTNLDLNKWTSDDNSDVEIAKVVGQYML 476
                               .:.::|.|..::.:  .|:.|..:....|..:|:.|:.....:.:.
Mouse   419 RRVTWCYGKMKDSNYEKEFKVPTSITTRKRKTSVTSLRENSLMKFGESAASSEFEMLNNTLESLA 483

  Fly   477 EIEELQTKLIESEEHRKQMEIKATTSPRNAKPVY-------------DSDIITKAKKDLEREREL 528
            |:|......:.|||:     :::..:..||:  |             :.|:..|.|:..|.|...
Mouse   484 EVEWSSATTLLSEEN-----VESELNSLNAQ--YNDLVLDYEQLRRENEDLKLKLKEKNELEEFE 541

  Fly   529 LMSRSLPGIQNQNVSSEETEVASSDSEAEEVVKDLEAIDNDIEMRTKLIEQLELTNSRYEQMRTH 593
            |:.:.....|...:..|.:.:.:....|||..:|||   ||:..:.||:::.|      ||::..
Mouse   542 LLEQKEERDQEMQLMHEVSNLKNLIKHAEEYNQDLE---NDLSSKVKLLKEKE------EQIKNL 597

  Fly   594 YEEKLSVLYCKIENTQKERDDVLANMTTSVSTPSKDSLK------KVKTDYESKISHMQTEIRKL 652
            .|      |...:.::|.:.|:  :.|:..:...|.:::      .|..|.:.:.:.:::|..:|
Mouse   598 QE------YIDAQKSEKMKIDL--SYTSDATEDLKQAMRTLSDLDTVALDAKKESAFLRSENLEL 654

  Fly   653 QNAQREHIRSKQQLKSHEVRIGTLRNELNELKFAKVKLMKKMSQQSNRHKQEESRKS-------- 709
            :....|...|::|::|.   |...:.:|.    ||.|:...:.::.....||.|:.|
Mouse   655 KEKINELSDSRKQMESD---IQMYQRQLE----AKKKMQTDLDKELQLAFQEISKLSALVDGKGL 712

  Fly   710 -------KEIAQLLKEQRRQKNAVLTLEAKVSAKEQILKRKTEEVIALRKSQRGKSGQ------- 760
                   |.|..|.||..::.....||:.:|:...: ||....||..||:....||.:
Mouse   713 LSNLELEKRITDLQKELNKEAEEKQTLQEEVNLLSE-LKSLPSEVETLRRELYEKSEELHIITTE 776

  Fly   761 -----RAPAHLTSKIA--------TLDGFST-----RSARHRWENLYRNILHAARNRQLITQLEK 807
                 ...||..|:|.        |.|..:|     |.:...|:.|  ..|||        :||.
Mouse   777 REKLFSEMAHKDSRIQGLLEEIGNTRDDLATSQLSRRGSDGEWQAL--ESLHA--------ELEH 831

  Fly   808 ELERLILEREDLSRELNIMENGQTADRQTDEFNEVDNLKTNISYIQENIEHVQQAIMEFEDTKDT 872
            ....::.|||.|.:|:..:.            .|.::|..::..::..:.|..|   |.|.....
Mouse   832 RHAGVLEERERLKQEIGALS------------KEAESLAFSLDSVKAELSHKTQ---ELEQKTVE 881

  Fly   873 VQSHVNKIQSLLDEVSTVAEAKFILQKFSDTAIVMSCNLAIAESHLQEKESLLKEVK----QESG 933
            .|..:||:::|.:|:.:...:   ||......::::          ::.:..|||||    ::..
Mouse   882 GQERLNKMEALREELESRDSS---LQSVEKEKVLLT----------EKLQQALKEVKALTQEKKN 933

  Fly   934 IQQQILQHFLSQNSNVHIADIFDSLNLKGSNIASSVNPGSQKSLISNATYDIPQDDKNSEFHQVE 998
            ::|  ||..|....:...:||.|::|:         |..:|:.|: ||...:.|       ||..
Mouse   934 LKQ--LQESLQTERDQLRSDIQDTVNM---------NIDTQEQLL-NALESLKQ-------HQET 979

  Fly   999 MRRTTSRSPSPLGNS-----------DPFDKSPKVRRRTAKRQDLLFG--DTELSEQ 1042
            :.....::...|.::           :...|...:..:......||.|  |.|::|:
Mouse   980 INMLKMKAAEELSDNLHVKDRGGARDEAQQKMDGIDEQNESAHTLLGGGKDNEVTEE 1036

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp31E	NP_609398.2	KISc_KIF4	12..364	CDD:276823	138/362 (38%)
		Mplasa_alph_rch	<372..>748	CDD:275316	88/450 (20%)
		SMC_prok_B	622..>899	CDD:274008	66/322 (20%)
		Rcc_KIF21	798..870	CDD:410202	13/71 (18%)
Cenpe	NP_776123.3	KISc_CENP_E	6..329	CDD:276825	138/360 (38%)
		SMC_prok_B	586..1436	CDD:274008	105/530 (20%)
		SMC_prok_B	1080..1896	CDD:274008
		SMC_prok_B	1712..>2105	CDD:274008
		Kinetochore binding domain. /evidence=ECO:0000250|UniProtKB:Q02224	1959..2249
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2193..2214
		Globular autoinhibitory domain. /evidence=ECO:0000250	2283..2468
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2368..2438