Protein Alignment Klp31E and klp-12

DIOPT Version :9

• Sequence 1: NP_001162935.1
• Gene: Klp31E / 34422
• FlyBaseID: FBgn0032243
• Length: 1048
• Species: Drosophila melanogaster
• Sequence 2: NP_001023349.1
• Gene: klp-12 / 178057
• WormBaseID: WBGene00002223
• Length: 1605
• Species: Caenorhabditis elegans
• Alignment Length: 1127
• Identity: 412/1127 - (36%)
• Similarity: 646/1127 - (57%)
• Gaps: 148/1127 - (13%)

  Fly    11 DSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGSDKAFTFDYVFDTNSNQCDIYSDCVEK 75
            |:.|:||:|||||.:||.::..|:||:|...:||:.:|.|::||:|:|||..:.|..:|..||||
 Worm     3 DTCVQVALRIRPQGNREKLEGSRVCTSVLPNDPQVTIGGDRSFTYDHVFDMPTLQYVVYESCVEK 67

  Fly    76 LVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFSGIEQLEGSST 140
            |||....|||||||||||||||||:||||.|| .:.:.....||:||||::|.|..|.:.:..:.
 Worm    68 LVDGLFDGYNATVLAYGQTGSGKTHTMGTAFD-AAVTQKEEDLGVIPRAIQHTFRKIAECKAQAI 131

  Fly   141 SEHPAAGG--SPQFSLAVQYIELYNEDIFDLL-DPFNKNSNFKIHEDASGQITISGASIKPIYQP 202
            .:     |  .|.|.::||::||||:|:.||| |..:.:|:.:||||:.|:|.:.|...:.::..
 Worm   132 EQ-----GLLEPAFEVSVQFVELYNDDVLDLLSDDRSMSSSIRIHEDSRGEIVLHGVEQRSVFDM 191

  Fly   203 HDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQRL-LTPSDNVPDN-----DLETLTSK 261
            |..:..|:.|||.||.|:|.||:|||||||:||:.:::||: ..|.|...:.     ::|.|.:|
 Worm   192 HGTMDILKNGALNRTVAATNMNEQSSRSHAIFTLHLKQQRVAANPLDESGEQKTGELEMEMLCAK 256

  Fly   262 FHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRLLQD 326
            |||||||||||:|||.|||:||:||||||.|||||||.|:|||..:.:..|||||||||||||||
 Worm   257 FHFVDLAGSERMKRTGATGDRAKEGISINVGLLALGNVIAALGGANGKVSHVPYRDSKLTRLLQD 321

  Fly   327 SLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKINQDQSSRTISQLRREIAALQLE 391
            ||||||:||||||.||||.||:|||||:||||||:.|||||..|||:||:.|.:||..||||:.|
 Worm   322 SLGGNSRTLMIACCSPSDSDFVETLNTMKYANRAKEIKNKVVANQDKSSKMIGELRSRIAALEAE 386

  Fly   392 LLEYKQGKLIVDCEGNTTISDTFNENKLLLSEIKRLQQRLKSLQSTIATLTQRNADLKTNLDLNK 456
            |||:|||:..||.:|:..::|.::||..|.||:..|:.|:|:|..|:..|...|.|||...:.|.
 Worm   387 LLEFKQGRRTVDVDGHEVVNDQYHENVYLTSEVNHLRFRVKALNETLDILRTENIDLKAKQEFNS 451

  Fly   457 WTS-------DDNSDVE-IAKVVGQYMLEIEELQTKLIESEEHRKQME--------IKATTSPRN 505
            ..|       ....:|: |.....:|:.|:|..::.|.||:....|:.        :.|:.....
 Worm   452 IASLPTAGSGGAEGEVDAIQSTFRKYLEELERTKSLLYESQSTCDQLRKDNARWKALGASRGSGG 516

  Fly   506 AKPVYDSD-IITKAKKDLERERELLMSRSLPGIQNQNVSSEETEVASSD----SEAEEVVKDLEA 565
            ....::|. :|..||:::|::|:|:.|.::.|   :|||||.:.:|..:    :||||:: |.|.
 Worm   517 GNAEFNSQKLIEMAKQEVEKQRKLMESVNIGG---ENVSSEYSSMAQDEDGTSNEAEELL-DEED 577

  Fly   566 IDND---------------------------IEMRTKLIEQLELTNSRYEQMRTHYEEKLSVLYC 603
            :|.|                           ::::.|||:|||....:.:|:|..||:||..|..
 Worm   578 LDEDEDETAEEKQEQEESEALQIDLSEVMIELDIKEKLIDQLERAERQNQQIRETYEKKLRELME 642

  Fly   604 KIENTQKERDDVLANMTTSVSTPSKDSLKKVKTDYESKISHMQTEIRKLQNAQREHIR--SKQQL 666
            :|::|:.|||.||..  ......:.:.:|.:|.:||.||:.::.|::|::...:||::  :|.|.
 Worm   643 RIKDTETERDRVLNE--GGKRGGNNEQMKAIKQEYELKITDLRKELKKIEALDKEHLKVIAKSQR 705

  Fly   667 KSHEVRIGTLRNELNELKFAKVKLMKKMSQQSNRHKQEESRKSKEIAQLLKEQRRQKNAVLTLEA 731
            :..|..  .|::|:.:||.|||:|:|||::...:.|.::...::..|...|:.|.|.|.:.|||.
 Worm   706 ELQEKT--RLKSEVVDLKKAKVELIKKMNEDKKKQKTQQLANARAFATKEKQTRLQANKIRTLEM 768

  Fly   732 KVSAKEQILKRKTEEVIALRKS---------QRGKSGQRAPAHLTSKIA-----TLDG------- 775
            |...:||.||:.|:||.||||.         |..:...|..|.:|:..|     .:.|
 Worm   769 KDKQREQFLKKTTQEVNALRKEKAVAAATARQANRGTPRGGAAVTNSPARRVRGVVGGVQAIKEL 833

  Fly   776 -FSTRSARHRWENLYRNILHAARNRQLITQLEKELERLILEREDLSRELNIMENGQTADRQTDE- 838
             ||.::::.:|:.:.|.|..:||.||::.::|.||||.:.||..:..|  |:||.:...:..|.
 Worm   834 AFSAKASKVKWDVIVRKIEESARRRQIVQKMEAELERYLNERHAVMVE--IVENEKQFTQSQDVI 896

  Fly   839 -----FNEVDNLKTNISYIQENIEHVQQAIMEFEDTKDTVQSHVNK------------IQSLLDE 886
                 ...:|:.|..:.|:|:.|.:.|:.|.:.::  |...|:...            |:.|.|.
 Worm   897 YRDGLLEAIDSAKQKLQYVQDQITYQQKLICDVDE--DITASNAENEPILDVGLKKQTIKQLFDG 959

  Fly   887 VSTVAEAKFILQKFSDTAIVMSCNLAIAESHLQEKESLLKEVKQESGIQQQILQHFLSQNSNVHI 951
            ..|::||:::||...|..|..:...|..||..:|..:.:::::|:|.:::|:|...:...:.|..
 Worm   960 CDTLSEARYLLQHLFDLCIDKAALAAKVESEFKECAARIEQLEQQSSLKEQLLTSIIEDKNLVDE 1024

  Fly   952 ADIFDSLNLKGSNIASSVNPGSQKSLISNATYDIPQDDKNSEFHQVEMRRTTSRSPSPLGNSDPF 1016
            .:.|...:|:.|..:      ||.||:.:.:..:.:|     .|.::
 Worm  1025 IEGFVPSDLRKSRTS------SQSSLLRSGSPSVVED-----AHTLQ------------------ 1060

  Fly  1017 DKSPKVRRRTAKRQDLLFGDTE 1038
              :.||||.||.:::|||.::|
 Worm  1061 --NYKVRRHTATQEELLFANSE 1080

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp31E	NP_001162935.1	KISc_KIF4	12..364	CDD:276823	194/360 (54%)
		KISc	13..370	CDD:214526	199/365 (55%)
		KASH_CCD	409..589	CDD:291334	57/227 (25%)
		RasGAP	<685..750	CDD:295371	24/64 (38%)
klp-12	NP_001023349.1	KISc_KIF4	4..359	CDD:276823	194/360 (54%)
		KISc	6..365	CDD:214526	199/364 (55%)
		ATP-synt_B	<609..739	CDD:304375	44/133 (33%)
		WD40	1277..1601	CDD:238121
		WD40	<1287..1603	CDD:225201
		WD40 repeat	1288..1322	CDD:293791
		WD40 repeat	1327..1364	CDD:293791
		WD40 repeat	1373..1429	CDD:293791
		WD40 repeat	1439..1482	CDD:293791
		WD40 repeat	1489..1524	CDD:293791
		WD40 repeat	1531..1556	CDD:293791

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160164619
Domainoid	1	1.000	373	1.000	Domainoid score	I467
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H56761
Inparanoid	1	1.050	646	1.000	Inparanoid score	I431
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG28834
OrthoDB	1	1.010	-	-		D390391at2759
OrthoFinder	1	1.000	-	-		FOG0002395
OrthoInspector	1	1.000	-	-		oto18314
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	1	1.100	-	-	LDO	PTHR24115
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R5505
SonicParanoid	1	1.000	-	-		X2095
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	15	14.940