DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Klp31E and Kif4

DIOPT Version :9

Sequence 1:	NP_001162935.1	Gene:	Klp31E / 34422	FlyBaseID:	FBgn0032243	Length:	1048	Species:	Drosophila melanogaster
Sequence 2:	NP_032472.2	Gene:	Kif4 / 16571	MGIID:	108389	Length:	1231	Species:	Mus musculus

Alignment Length:	1028	Identity:	308/1028 - (29%)
Similarity:	527/1028 - (51%)	Gaps:	123/1028 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MISEVDSINKDSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGSDKAFTFDYVFDTNSNQ 65
            |..||..|    .||||:|.||..|:|:.:.|:.|.:...||||:.:|:||:||:|:|||.::.|
Mouse     1 MKEEVKGI----PVRVALRCRPLVSKEIKEGCQTCLSFVPGEPQVVVGNDKSFTYDFVFDPSTEQ 61

  Fly    66 CDIYSDCVEKLVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFS 130
            .::::..|..|:.....||||||||||||||||||:||..:..|.|...::  |:|||.::.:|.
Mouse    62 EEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQEHDSAI--GVIPRVIQLLFK 124

  Fly   131 GIEQLEGSSTSEHPAAGGSPQFSLAVQYIELYNEDIFDLL-DPFNKNSNFKIHEDASGQITISGA 194
            .|.:....            :|:|.|.|:|:|||:|.||| ....|.:...|.||....|.|.|.
Mouse   125 EINKKSDF------------EFTLKVSYLEIYNEEILDLLCSSREKATQINIREDPKEGIKIVGL 177

  Fly   195 SIKPIYQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQRLLTPSDNVPDNDL-ETL 258
            :.|.:....|.:..|:||..:||.|||.||.|||||||:|||.:.:::         .||. .:.
Mouse   178 TEKTVLVASDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISIEQRK---------KNDKNSSF 233

  Fly   259 TSKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTRL 323
            .||.|.||||||||.|:|:|.|:|.||||:||.|||.|||.|||||| .|:...|||||||||||
Mouse   234 RSKLHLVDLAGSERQKKTKAEGDRLREGININRGLLCLGNVISALGD-DKKGNFVPYRDSKLTRL 297

  Fly   324 LQDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKINQDQSSRTISQLRREIAAL 388
            |||||||||.|||||||||:|.:..||||||:||:|||.||||..||.|..:..::.|::::..|
Mouse   298 LQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIINIDPQAAELNHLKQQVQQL 362

  Fly   389 QLELLEYKQGKL--IVDCEGNTTISDTFNENKLLLSEIKRLQQRLKSLQSTIATLTQR-----NA 446
            |:.||:...|.|  .::.|.:..:.....:|:.|:.|.::|.:.|.......|.:.:|     .|
Mouse   363 QILLLQAHGGTLPGDINVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQA 427

  Fly   447 DLKTNLDLNKWTSDDNSDVEIAKVVGQYMLEIEELQTKLIESEEHRKQMEIKATTS------PRN 505
            :.|.|..|.:........|::.|:|            :.:|.:|.::.:||.....      ...
Mouse   428 NEKMNAKLEELRRHAACKVDLQKLV------------ETLEDQELKENIEIICNLQQVIAQLSDE 480

  Fly   506 AKPVYDSDIITKAKKDLERERELLMSRSLPGIQNQNVSSEETEVASSDS--EAEEVVKDLEAIDN 568
            |.....:.|.|..:.|.:             :|:...:|..::|.|:..  ...::.|:|..::.
Mouse   481 AAACMTATIDTAGEADTQ-------------VQSSPDTSRSSDVFSTQHALRQAQMSKELIELNK 532

  Fly   569 DIEMRTKLIEQLELTNSRYEQMRTHYEEKLSVLYCKIENTQKERDDVLANMTTSVSTPSKDSLK- 632
            .:.::..|.:::...:::.:.::..|::.:..|..::.:.|:|:::::..:.|:    .||:.:
Mouse   533 ALALKEALAKKMTQNDNQLQPIQFQYQDNIKNLESEVLSLQREKEELVLELQTA----KKDANQA 593

  Fly   633 KVKTDYESKISHMQTEIRKLQNAQREHIRSKQQLKSHEVRIGTLRNELNELKFAKVKLMKKMSQQ 697
            |:......::..::.:|..|:...:|..:..:..:|.|..:..|..|:..:|..:|:||::|.:.
Mouse   594 KLSERRRKRLQELEGQIADLKKKLQEQSKLLKLKESTEHTVSKLNQEIRMMKNQRVQLMRQMKED 658

  Fly   698 SNRHKQEESRKSKEIAQLLKEQRRQKNAVLTLEAKVSAKEQILKRKTEEVIALRKSQRGKSGQRA 762
            :.:.:|.:.:|.||:.||.:..|:::..:|.||.....:..:|:|||||..|..|  |.|...:.
Mouse   659 AEKFRQWKQQKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANK--RLKDALQK 721

  Fly   763 PAHLTSKIATLDGFSTRSARHRWENLYRNILHAARNRQLITQLEKELERLILEREDLSRELNIM- 826
            ...:..|..........|...|.:|...|.:....:.:   :.::.|..|:.||:.|::::..:
Mouse   722 QKEVAEKRKETQSRGMESTAARMKNWLGNEIEVMVSTE---EAKRHLNGLLEERKILAQDVAQLK 783

  Fly   827 ---ENGQTAD----RQTDEFNE---------------VDNLKTNISYIQENIEHVQQAIMEFEDT 869
               |:|:...    |:|..::|               :::|:|.:......|..:||.:::.| :
Mouse   784 EKRESGENPPLKLRRRTFSYDEIHGQDSGAEDSIAKQIESLETELELRSAQIADLQQKLLDAE-S 847

  Fly   870 KDTVQSHVNKIQSLLDE-------VSTVAEAKFILQKFSD-------TAIVMSCNLAIAESHLQE 920
            :|..:.....|.::|:.       |..:..:|.::.|...       :.|.:...|...::|..:
Mouse   848 EDRPKQRWESIATILEAKCAIKYLVGELVSSKILVSKLESSLNQSKASCIDVQKMLFEEQNHFAK 912

  Fly   921 KESLLKE--VKQESGIQQQILQHFLS--QNSNVHIADIFDSLNLKGSNIASSV 969
            .|:.|||  ||.|...|:::| :.||  |.|.:....:.:|::.|...:.|::
Mouse   913 IETELKEELVKVEQQHQEKVL-YLLSQLQQSQMTEKQLEESVSEKEQQLLSTL 964

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Klp31E	NP_001162935.1	KISc_KIF4	12..364	CDD:276823	174/353 (49%)
		KISc	13..370	CDD:214526	178/358 (50%)
		KASH_CCD	409..589	CDD:291334	29/192 (15%)
		RasGAP	<685..750	CDD:295371	20/64 (31%)
Kif4	NP_032472.2	KISc_KIF4	8..338	CDD:276823	175/357 (49%)
		KISc	9..344	CDD:214526	178/358 (50%)
		SbcC	407..995	CDD:223496	111/594 (19%)
		Globular	1001..1231
		Pre-SET	<1070..1120	CDD:282838
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1189..1212
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0244
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D74122at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.720