DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Klp31E and Kif21b

DIOPT Version :9

Sequence 1:	NP_001162935.1	Gene:	Klp31E / 34422	FlyBaseID:	FBgn0032243	Length:	1048	Species:	Drosophila melanogaster
Sequence 2:	NP_001034561.1	Gene:	Kif21b / 16565	MGIID:	109234	Length:	1624	Species:	Mus musculus

Alignment Length:	1172	Identity:	469/1172 - (40%)
Similarity:	657/1172 - (56%)	Gaps:	195/1172 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 DSSVRVAVRIRPQNSRELIDMCRICTTVTLGEPQIFLGSDKAFTFDYVFDTNSNQCDIYSDCVEK 75
            |..|:||||||||.|:|.|:.|.|||:||.||||:.||.|||||:|:|||.::.|..|||.||.|
Mouse     6 DCCVKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLLGKDKAFTYDFVFDLDTWQEQIYSTCVSK 70

  Fly    76 LVDSTLHGYNATVLAYGQTGSGKTYTMGTGFDHESESSDSVQLGIIPRAVRHIFSGIEQLEGSST 140
            |::....|||||||||||||:|||||||||||  :.:|:..| ||||||:.|:|.||:  |....
Mouse    71 LIEGCFEGYNATVLAYGQTGAGKTYTMGTGFD--TVTSEEEQ-GIIPRAIAHLFRGID--ERKRR 130

  Fly   141 SEHPAAGGSPQFSLAVQYIELYNEDIFDLLDPF------NKNSNFKIHEDASGQITISGASIKPI 199
            ::.....| |:|.::.|::|||||:|.||.|..      ::.||.||||||:|.|..:|.:.:.|
Mouse   131 AQEKGVTG-PEFKVSAQFLELYNEEILDLFDSTRDPDARHRRSNIKIHEDANGGIYTTGVTSRLI 194

  Fly   200 YQPHDALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQRLLTPSD-------NVPD----- 252
            ....:.::.|:||||:||||||:||.|||||||:|||.:.:.|:....|       .:||
Mouse   195 NSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTIHLCQMRVCAQPDLVNETVTGLPDGAAPT 259

  Fly   253 -NDLETLTSKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYR 316
             .:.||||:||||||||||||||||.||||||:||||||||||||||.||||||:||:.:|||||
Mouse   260 GTEYETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDQSKKVVHVPYR 324

  Fly   317 DSKLTRLLQDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKINQDQSSRTISQL 381
            ||||||||||||||||||:||||||||||||||||||||||||||||||||.:|||::|:.||.|
Mouse   325 DSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQISAL 389

  Fly   382 RREIAALQLELLEYKQGKLIVDCEGNTTISDTFNENKLLLSEIKRLQQRLKSLQSTIATLTQRNA 446
            |.|||.||:||:|||.||.::..:|....||.|.||.:|..|...|:.|:|::|..|..:..|..
Mouse   390 RAEIARLQMELMEYKAGKRVIGEDGTEGYSDLFRENAMLQKENGALRLRVKAMQEAIDAINNRVT 454

  Fly   447 DL---KTNLDLNKWTSDDNSDVEIAKVVGQYMLEIEELQTKLIESEEHRKQMEIK-ATTSPRN-- 505
            .|   :.||.|.| ..|.|.  .|..::..|:.|||||:|||:|||...:.:... :..|.||
Mouse   455 QLMSQEANLLLAK-AGDGNE--AIGALIQNYIREIEELRTKLLESEAMNESLRRSLSRASARNPY 516

  Fly   506 ------AKPVYD----------SDIITKAKKDLER-------------ERELLMSRSLPGIQN-- 539
                  |.|.:.          |::|.|||:||||             |:|....|:....:|
Mouse   517 SLGASPAGPAFGGSPATSMEDASEVIRKAKQDLERLKKKEVRQRRKSPEKEAFKKRAKLQAENSE 581

  Fly   540 ---QNVSSEETE-------------------------VASSDSEAEE----VVKDLEAIDNDIEM 572
               :|.:.||.|                         :..|||:.||    ...||..:..:||:
Mouse   582 ETDENEAEEEEEERDESGCEEEEGREDEDEDSGSEESLVDSDSDPEEKEVNFQADLADLTCEIEI 646

  Fly   573 RTKLIEQLELTNSRYEQMRTHYEEKLSVLYCKIENTQKERDDVLANMTTSVSTPSKDSLKKVKTD 637
            :.|||::||.:..|.:.::..|||||.:|..||.:||.|||.||.|::| :...:::...|:|.|
Mouse   647 KQKLIDELENSQRRLQTLKHQYEEKLILLQNKIRDTQLERDRVLQNLST-MECYTEEKANKIKAD 710

  Fly   638 YESKISHMQTEIRKLQNAQREHIRSKQQLKSHEVRIGTLRNELNELKFAKVKLMKKMSQQSNRHK 702
            ||.::..|..:::|||.||:||.|..:....:|..:..|:.|:.|:|.|||.|||:|.::..|.:
Mouse   711 YEKRLREMNRDLQKLQAAQKEHARLLKNQSRYERELKKLQAEVAEMKKAKVALMKQMREEQQRRR 775

  Fly   703 QEESRKSKEIAQLLKEQRRQKNAVLTLEAKVSAKEQILKRKTEEVIALRK-----SQR--GKSGQ 760
            ..|:::::|||||.||||||:..:..||::...:|.:|:|||:||.|||:     |:|  |:.|.
Mouse   776 LVETKRNREIAQLKKEQRRQEFQIRALESQKRQQEIVLRRKTQEVSALRRLAKPMSERVAGRVGL 840

  Fly   761 RAP---------AHLTS-------------------KI---------ATLDG------------- 775
            :.|         |..||                   ||         ||::|
Mouse   841 KPPNMDSGAEVSASTTSSEAESGARSVSSIVRQWNRKIDHFLGDRPTATVNGGRPARKKFQKKGA 905

  Fly   776 ---FSTRSARHRWENLYRNILHAARNRQLITQLEKELERLILEREDL---------SRELNIMEN 828
               || ::||.:|::|.|.|:.....|..|..||.::||||.:||:|         .||....|:
Mouse   906 SQSFS-KAARLKWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELFLLQEALRRKREHLQAES 969

  Fly   829 GQTADRQTDEFNEVDNLKTNISYIQENIEHVQQAIMEFEDTKDTVQSHVNKIQSLLDEVSTVAEA 893
            .:......:...|::.|..||.||.::|...|..|::.|:||:.:.|   ...|::....::|||
Mouse   970 PEEEKGLQELAEEIEVLAANIDYINDSITDCQATIVQLEETKEELDS---TDTSVVISSCSLAEA 1031

  Fly   894 KFILQKFSDTAIVMSCNLAIAESHLQEKESLLKEVKQESGIQQQILQHFLSQNSNVHIADIFDSL 958
            :.:|..|...:|.....:|..|:.::..|..|::.......|..:|...|.:.:..|
Mouse  1032 RLLLDNFLKASIDKGLQVAQKEAQIRLLEGRLRQTDMTGSSQNHLLLDALREKAEAH-------- 1088

  Fly   959 NLKGSNIASSVNPGSQKSLISNATYD---IPQDDKNSEFHQVEMRRTTSRSPSPLGNSDPFDKSP 1020
                        |..| :||.|..::   ...|::.|||.:....::.:...|...:...|...|
Mouse  1089 ------------PELQ-ALIYNVQHENGYASTDEEVSEFSEGSFSQSFTMKGSTSHDDFKFKGEP 1140

  Fly  1021 KV 1022
            |:
Mouse  1141 KL 1142

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Klp31E	NP_001162935.1	KISc_KIF4	12..364	CDD:276823	228/370 (62%)
		KISc	13..370	CDD:214526	233/375 (62%)
		KASH_CCD	409..589	CDD:291334	70/248 (28%)
		RasGAP	<685..750	CDD:295371	29/64 (45%)
Kif21b	NP_001034561.1	KISc_KIF4	7..372	CDD:276823	228/370 (62%)
		KISc	9..378	CDD:214526	233/374 (62%)
		Interaction with TRIM3. /evidence=ECO:0000269\|PubMed:24086586	401..1100		212/727 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	553..629		12/75 (16%)
		PKK	661..795	CDD:289257	54/134 (40%)
		JAKMIP_CC3	679..807	CDD:292653	51/128 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	837..866		5/28 (18%)
		Prefoldin	965..1101	CDD:298833	34/159 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1199..1253
		WD40	1302..1615	CDD:238121
		WD40	1308..>1617	CDD:225201
		WD 1	1308..1345
		WD40 repeat	1314..1348	CDD:293791
		WD 2	1348..1386
		WD40 repeat	1353..1389	CDD:293791
		WD40 repeat	1409..1452	CDD:293791
		WD 3	1412..1450
		WD 4	1453..1495
		WD40 repeat	1458..1502	CDD:293791
		WD 5	1504..1541
		WD40 repeat	1511..1543	CDD:293791
		WD 6	1545..1584
		WD40 repeat	1550..1574	CDD:293791
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	425	1.000	Domainoid score	I614
eggNOG	1	0.900	-	-		E2759_KOG0244
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	697	1.000	Inparanoid score	I674
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002395
OrthoInspector	1	1.000	-	-		otm44325
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R5505
SonicParanoid	1	1.000	-	-		X2095
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	12	11.750