Protein Alignment Klp31E and Kif21a

DIOPT Version :10

• Sequence 1: NP_609398.2
• Gene: Klp31E / 34422
• FlyBaseID: FBgn0032243
• Length: 1048
• Species: Drosophila melanogaster
• Sequence 2: XP_017171930.1
• Gene: Kif21a / 16564
• MGIID: 109188
• Length: 1685
• Species: Mus musculus
• Alignment Length: 1202
• Identity: 468/1202 - (38%)
• Similarity: 662/1202 - (55%)
• Gaps: 216/1202 - (17%)

  Fly    19 RIRPQNSRELIDMCRICTTVTLGEPQIFLGSDKAFTFDYVFDTNSNQCDIYSDCVEKLVDSTLHG 83
            |||||.::|.|:.|.|||:||.||||:|||.|||||||||||.:|.|..||:.|:|||::....|
Mouse    23 RIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEG 87

  Fly    84 YNATVLAYGQTGSGKTYTMGTGFD---HESESSDSVQLGIIPRAVRHIFSGIEQLEGSSTSEHPA 145
            |||||.||||||:|||||||||||   .|.|.      |||.|||||:|..|::   ..||....
Mouse    88 YNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQ------GIISRAVRHLFKSIDE---KKTSAIKN 143

  Fly   146 AGGSPQFSLAVQYIELYNEDIFDL------LDPFNKNSNFKIHEDASGQITISGASIKPIYQPHD 204
            ....|:|.:..|::|||||::.||      :|..||.||.:||||::|.|...|.:.:.:....:
Mouse   144 GLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPE 208

  Fly   205 ALKYLQQGALARTTASTKMNDQSSRSHALFTIFVRRQRLL--TPSDNVPDNDL----------ET 257
            .::.|:.|||:||||||:||.|||||||:|||.|.:.|:.  |.::|..||.|          ||
Mouse   209 MMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFET 273

  Fly   258 LTSKFHFVDLAGSERLKRTQATGERAREGISINCGLLALGNCISALGDKSKRALHVPYRDSKLTR 322
            ||:||||||||||||||||.||||||:||||||||||||||.|||||||||||.|||||||||||
Mouse   274 LTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTR 338

  Fly   323 LLQDSLGGNSQTLMIACVSPSDRDFMETLNTLKYANRARNIKNKVKINQDQSSRTISQLRREIAA 387
            ||||||||||||:||||||||||||||||||||||||||||||||.:|||::|:.|:.||.||..
Mouse   339 LLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITR 403

  Fly   388 LQLELLEYKQGKLIVDCEGNTTISDTFNENKLLLSEIKRLQQRLKSLQSTIATLTQRNADLKTNL 452
            ||:||:|||.||.|:|.||..:|:|.|:||.:|.:|...|:.|:|::|.|:..|..|...|.:..
Mouse   404 LQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQ 468

  Fly   453 DLNKWTSDDNSDVEIAKVVGQYMLEIEELQTKLIESE---EHRKQMEIKAT-------TSPRNAK 507
            ...........:.||:.::..|:.|||:|:.||:|||   |:.::...:||       .|...:.
Mouse   469 ANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSP 533

  Fly   508 PVYDSD-----IITKAKKDLER---------------ERELLMSRSLPG---------------- 536
            .:..||     ||..||||||:               |......||:.|
Mouse   534 TILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKG 598

  Fly   537 -----------IQNQNVSSEETEVA------------------SSDSEAEEVVK---DLEAIDND 569
                       .:||.||..|.|..                  .||||::|...   ||..|..:
Mouse   599 VSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCE 663

  Fly   570 IEMRTKLIEQLELTNSRYEQMRTHYEEKLSVLYCKIENTQKERDDVLANMTTSVSTPSKDSLKKV 634
            |.::.|||::||.:..|.:.::..|||||.:|..||.:||.|||.||.|: .||.:.|::..|||
Mouse   664 IAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNL-GSVESYSEEKAKKV 727

  Fly   635 KTDYESKISHMQTEIRKLQNAQREHIRSKQQLKSHEVRIGTLRNELNELKFAKVKLMKKMSQQSN 699
            |.:||.|:..|..|:::||.||:||.|..:....:|.::..|:.::.|:|..||:|||:|.::..
Mouse   728 KCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQE 792

  Fly   700 RHKQEESRKSKEIAQLLKEQRRQKNAVLTLEAKVSAKEQILKRKTEEVIALRKSQR-------GK 757
            :.:..|||:::|||||.|:||::.:.:..|||:...:|.:|:||||||.|||:..|       ||
Mouse   793 KARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGK 857

  Fly   758 -----SGQRAPAHLTSKIA-----------------------------TLDG------------- 775
                 |...:||..|...|                             |.:|
Mouse   858 VTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGR 922

  Fly   776 -FSTRSARHRWENLYRNILHAARNRQLITQLEKELERLILEREDLS---------RELNIMENGQ 830
             |::::||.:|:.|.|.:......:..|:.:|.::.||:.:||:|:         ||..:.|:|:
Mouse   923 VFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGE 987

  Fly   831 TADRQTDEFNEVDNLKTNISYIQENIEHVQQAIMEFEDTKDTVQSHVNKIQSLLDEVSTVAEAKF 895
            ......:...|:::|..||.||.::|...|..||:.|:.|:  :.....:.:::: ..|:.||::
Mouse   988 GDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKE--EGETLDVTAVIN-ACTLTEARY 1049

  Fly   896 ILQKFSDTAIVMSCNLAIAESHLQEKESLLKEVKQESGIQQQILQHFLSQNSNVHIADIFDSLNL 960
            :|..|....|......|..|:.::..|..||:.:..|..|.|:|.|.|.:.
Mouse  1050 LLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEK-------------- 1100

  Fly   961 KGSNIASSVNPGSQKSLISNATYDI----PQDDKNSEFHQVEMRRTTSRSPSPLGNSDPFD---- 1017
                  :.:|| ...:|:.:|..|:    |:::::|......:     .||...|::...|
Mouse  1101 ------AELNP-ELDALLGHALQDLDGAPPENEEDSSEEDGPL-----HSPGSEGSTLSSDLMKL 1153

  Fly  1018 -----KSPKVRRRTAKRQDLLFGD-TELSEQT 1043
                 ...|.||||..:.:||:.| :|::..|
Mouse  1154 CGEVKPKNKARRRTTTQMELLYADSSEVASDT 1185

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp31E	NP_609398.2	KISc_KIF4	12..364	CDD:276823	228/365 (62%)
		Mplasa_alph_rch	<372..>748	CDD:275316	152/453 (34%)
		SMC_prok_B	622..>899	CDD:274008	97/340 (29%)
		Rcc_KIF21	798..870	CDD:410202	22/80 (28%)
Kif21a	XP_017171930.1	KISc_KIF4	23..380	CDD:276823	228/365 (62%)
		Cast	<372..846	CDD:431111	170/474 (36%)
		EnvC	650..>865	CDD:443969	84/215 (39%)
		Rcc_KIF21A	946..1027	CDD:410204	22/80 (28%)
		WD40	1350..1663	CDD:238121
		WD40 repeat	1401..1460	CDD:293791
		WD40 repeat	1466..1501	CDD:293791
		WD40 repeat	1506..1550	CDD:293791
		WD40 repeat	1558..1591	CDD:293791
		WD40 repeat	1598..1634	CDD:293791