DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Npc1a and Npc1l1

DIOPT Version :9

Sequence 1:	NP_001188769.1	Gene:	Npc1a / 34358	FlyBaseID:	FBgn0024320	Length:	1287	Species:	Drosophila melanogaster
Sequence 2:	NP_001002025.1	Gene:	Npc1l1 / 432367	RGDID:	1303135	Length:	1331	Species:	Rattus norvegicus

Alignment Length:	1323	Identity:	511/1323 - (38%)
Similarity:	767/1323 - (57%)	Gaps:	103/1323 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly    21 VLFTLIQSSKQD-----------CVWYGVCNTN-DFS------HSQNCPYNGTAKEMATDGLELL 67
            :|:.|:.|:.|.           |.:|..|..| :.|      .:.:|..|..|:.:..:.|.||
  Rat     9 LLWALLLSAAQGELYTPKHEAGVCTFYEECGKNPELSGGLTSLSNVSCLSNTPARHVTGEHLALL 73

  Fly    68 KKRCGFLLENSENKF-CCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMH 131
            ::.|..|.......| ||...|:..|..::.:...:|.|||:|.:|.|...|..||||.|:.|::
  Rat    74 QRICPRLYNGPNTTFACCSTKQLLSLESSMSITKALLTRCPACSDNFVSLHCHNTCSPDQSLFIN 138

  Fly   132 VVATQKNKKGD-EYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWF 195
            |....:...|: ..:.:.:......:..|.|:|||||.:|....||...|||.|.::.||..:|.
  Rat   139 VTRVVERGAGEPPAVVAYEAFYQRSFAEKAYESCSQVRIPAAASLAVGSMCGVYGSALCNAQRWL 203

  Fly   196 NFMGDATNPYVPFQITYIQHEP-KSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQ-GP 258
            ||.||..|...|..||:...|| ::..:...|||....|||::.......|||.||..|||. .|
  Rat   204 NFQGDTGNGLAPLDITFHLLEPGQALPDGIQPLNGKIAPCNESQGDDSAVCSCQDCAASCPVIPP 268

  Fly   259 PEPPRPEPF--KIVGLDAYFVIMAAVF--LVGVLVFLMGSFLFTQGSSMDDNFQVDGNDVSDEMP 319
            ||..||..:  ::.|..|..:|..|||  |..|||.|      ...|:.:.| :.:|...:.::|
  Rat   269 PEALRPSFYMGRMPGWLALIIIFTAVFVLLSAVLVRL------RVVSNRNKN-KAEGPQEAPKLP 326

  Fly   320 YSENDSYFEKLGAHTETFLETFFTKWGTYFASNPGLTLIAGASLVVI-LGYGINFIEITTDPVKL 383
            :.      .||..|  |.|..||..|||..||.| ||::|.:.:||| |..|:.|||:|||||:|
  Rat   327 HK------HKLSPH--TILGRFFQNWGTRVASWP-LTVLALSFIVVIALAAGLTFIELTTDPVEL 382

  Fly   384 WASPNSKSRLEREFFDTKFSPFYRLEQIIIKAVNLPQIVHNT-SNGPYTFGPVFDREFLTKVLDL 447
            |::|.|::|.|:.|.|..|.||:|..||.:.|.|.....::: ..|...|..:...:||.::|:|
  Rat   383 WSAPKSQARKEKSFHDEHFGPFFRTNQIFVTARNRSSYKYDSLLLGSKNFSGILSLDFLLELLEL 447

  Fly   448 QEGIKEINA------NGTQLKDICYAPLSDDGSEIDVSQCVVQSIWGYFGDDRE--RLDDHDEDN 504
            ||.::.:..      ....|:|||||||:...:.:  |.|.|.|:..||.::|.  .|..:...|
  Rat   448 QERLRHLQVWSPEAERNISLQDICYAPLNPYNTSL--SDCCVNSLLQYFQNNRTLLMLTANQTLN 510

  Fly   505 GFN--VTYLDALYDCISNPYL----------CLAPYGGPVDPAIALGGFLPPGDQLTGSTKFELA 557
            |..  |.:.|....|.:.|..          |:|.||.||.|.:|:||:        ..|.:..|
  Rat   511 GQTSLVDWKDHFLYCANAPLTFKDGTSLALSCMADYGAPVFPFLAVGGY--------QGTDYSEA 567

  Fly   558 NAIILTFLVKNH-HNKTDLENALTWEKKFVEFMTNYTKNNMSQYMDIAFTSERSIEDELNRESQS 621
            .|:|:||.:.|: .:...:..|..||:.|::.|.::.:|...:: .:||::|||:|||:||.:..
  Rat   568 EALIITFSLNNYPADDPRMAQAKLWEEAFLKEMESFQRNTSDKF-QVAFSAERSLEDEINRTTIQ 631

  Fly   622 DVLTILVSYLIMFMYIAISLGHVKEFKRVFIDSKITLGIGGVIIVLASVVSSVGVFGYIGLPATL 686
            |:....|||:|:|:||:::||......||.::||.|||:||||:||.:|::::|.:.|:|:|::|
  Rat   632 DLPVFAVSYIIVFLYISLALGSYSRCSRVAVESKATLGLGGVIVVLGAVLAAMGFYSYLGVPSSL 696

  Fly   687 IIVEVIPFLVLAVGVDNIFILVQTHQRDQRKPNETLEQQVGRILGKVGPSMLLTSLSESFCFFLG 751
            :|::|:||||||||.|||||.|..:||..|.|.|..|..:||.||.|.|||||.||||:.|||||
  Rat   697 VIIQVVPFLVLAVGADNIFIFVLEYQRLPRMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLG 761

  Fly   752 GLSDMPAVRAFALYAGVALIIDFLLQITCFVSLFTLDTKRREENRMDI-CCFIKGKKPDSITSNE 815
            .|:.|||||.|||.:|:|:|:|||||:|.||:|.:||:||:|.:|.|: |||...|.|.. ...|
  Rat   762 ALTPMPAVRTFALTSGLAIILDFLLQMTAFVALLSLDSKRQEASRPDVLCCFSTRKLPPP-KEKE 825

  Fly   816 GLLYKFFSSVYVPFLMKKIVRASVMVIFFAWLCFSIAIAPRIDIGLDQELAMPQDSFVLHYFQSL 880
            |||.:||..:|.|||:.:.:|..||::|......::.:...|::|||||||:|:||:::.||..|
  Rat   826 GLLLRFFRKIYAPFLLHRFIRPVVMLLFLTLFGANLYLMCNINVGLDQELALPKDSYLIDYFLFL 890

  Fly   881 NENLNIGPPVYFVLKGDLAYTNSSDQNLVCAGQYCNDDSVLTQIYLASRHSNQTYIARPASSWID 945
            |..|.:|||||||......:::.:..|..|:...|...|:..:|..||...:|:|:|..||||:|
  Rat   891 NRYLEVGPPVYFVTTSGFNFSSEAGMNATCSSAGCKSFSLTQKIQYASEFPDQSYVAIAASSWVD 955

  Fly   946 DYFDWAAAASSCCKY-----RKDSGDFCPHQDTS--CLRCNITKNSL--LRPEEKEFVKYLPFFL 1001
            |:.||...:||||:.     .||  :|||..|||  ||: |....:|  :||..::|.||||:||
  Rat   956 DFIDWLTPSSSCCRLYIRGPHKD--EFCPSTDTSFNCLK-NCMNRTLGPVRPTAEQFHKYLPWFL 1017

  Fly  1002 KDNPDDTCAKAGHAAYGGAVRYSNSHERLNIEASYFMAYHTILKSSADYFLALESARKISANITQ 1066
            .|.|:..|.|.|.|||..:|..|:..:   :.||.|||||..|::|.|:..||.::|.::||||.
  Rat  1018 NDPPNIRCPKGGLAAYRTSVNLSSDGQ---VIASQFMAYHKPLRNSQDFTEALRASRLLAANITA 1079

  Fly  1067 MLQGRLMSNGVPMASALTVEVFPYSVFYVFYEQYLTMWSDTLQSMGISVLSIFVVTFVLMGFDVH 1131
            .|:      .|| .:....|||||::..|||:||||:..:.:.::.:..:..|||.::|:|.|:.
  Rat  1080 DLR------KVP-GTDPNFEVFPYTISNVFYQQYLTVLPEGIFTLALCFVPTFVVCYLLLGLDMC 1137

  Fly  1132 SALVVVITITMIVVNLGGLMYYWNISLNAVSLVNLVMAVGISVEFCSHLVHSFATSKSVSQIDRA 1196
            |.::.:::|.||:|:..|||..|.||.|||||:|||.|||:||||.||:..|||.|...::::||
  Rat  1138 SGILNLLSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSFAVSTKPTRLERA 1202

  Fly  1197 ADSLSKMGSSIFSGITLTKFAGILVLAFAKSQIFQVFYFRMYLGIVVIGAAHGLIFLPVLLSYIG 1261
            .|:...|||::|:|:.:|.|.|||:|.||::|:.|:|:||:.|.|.::|..|||:||||:|||:|
  Rat  1203 KDATVFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLPVVLSYLG 1267

  Fly  1262 APVSNARLRYHSQAAAEHETALA 1284
            ..|:.|.::....|:   |.|:|
  Rat  1268 PDVNQALVQEEKLAS---EAAVA 1287

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Npc1a	NP_001188769.1	NPC1_N	31..274	CDD:293023	82/266 (31%)
		2A060601	33..1261	CDD:273337	500/1275 (39%)
		Sterol-sensing	651..804	CDD:289145	89/153 (58%)
Npc1l1	NP_001002025.1	NPC1_N	30..283	CDD:293023	80/252 (32%)
		2A060601	32..1267	CDD:273337	500/1275 (39%)
		Sterol-sensing	662..814	CDD:289145	88/151 (58%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1933
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG55095
OrthoDB	1	1.010	-	-		D31089at33208
OrthoFinder	1	1.000	-	-		FOG0001659
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101370
Panther	1	1.100	-	-	O	PTHR45727
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X943
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	9	8.830