DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Npc1a and Npc1

DIOPT Version :9

Sequence 1:	NP_001188769.1	Gene:	Npc1a / 34358	FlyBaseID:	FBgn0024320	Length:	1287	Species:	Drosophila melanogaster
Sequence 2:	NP_705888.2	Gene:	Npc1 / 266732	RGDID:	628693	Length:	1278	Species:	Rattus norvegicus

Alignment Length:	1317	Identity:	582/1317 - (44%)
Similarity:	810/1317 - (61%)	Gaps:	109/1317 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly    13 GVHILIAAVLFTLI----QSSKQDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRC-G 72
            |.|.....:|..|:    |...|.|||||.|.........||.|:|..|.:..||.:|:::.| |
  Rat     2 GAHHPALGLLLLLLLCPAQVFSQSCVWYGECGVAFGDKRYNCEYSGPPKPLPKDGYDLVQELCPG 66

  Fly    73 FLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQ- 136
            ....|.  ..|||..|::.|..|::|....|.|||||..||:...|:.||||.|::|::|.||:
  Rat    67 LFFGNV--SLCCDVQQLQTLKSNLQLPLQFLSRCPSCFYNLMTLFCELTCSPHQSQFLNVTATED 129

  Fly   137 ----KNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNF 197
                :.::....:..::.::...:.|..|.:|..|..|.:.:.|..|:||. .|..||.|.|..:
  Rat   130 YFDPETRENKTNVKELEYYVGRSFANAMYNACRDVEAPSSNEKALGLLCGR-DARACNATNWIEY 193

  Fly   198 MGDATNPYVPFQI-------TYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCP 255
            |.:..|...||.|       :.:..||..|:         |..||::|......|||.||...|.
  Rat   194 MFNKDNGQAPFTIIPVFSDLSVLGMEPMRNA---------TKGCNESVDEVTGPCSCQDCSAVCG 249

  Fly   256 QGPPEPPRPEPFKIVGLDAYFVIMAAVFLVGVLVFLMG---------SFLFTQGSSMDDNFQVDG 311
            ..|..||.|.|::|:||||.:|||...::..:::|..|         .:..::.:.:|.|.....
  Rat   250 PKPQPPPPPVPWRILGLDAMYVIMWVAYMAFLVLFFGGLLAVWCHRRRYFVSEYTPIDSNIAFSS 314

  Fly   312 NDVSDEMPYSENDSYFEKLGAHTETFLETFFTKWGTYFASNPGLTLIAGASLVVI--LGYGINFI 374
            |. ||:    ...|..:.||...:..|...|||||.:...||  |.|...|||.|  ...|:.|:
  Rat   315 NS-SDK----GEASCCDPLGVAFDDCLRRMFTKWGAFCVRNP--TCIIFFSLVFIAACSSGLVFV 372

  Fly   375 EITTDPVKLWASPNSKSRLEREFFDTKFSPFYRLEQIIIKAVNLPQIVHN--TSNGPYTFGPVFD 437
            ::||:||:||::|:|::|||:|:||..|.||:|.||:||:|.|....::.  .|.....|||..:
  Rat   373 QVTTNPVELWSAPHSQARLEKEYFDKHFGPFFRTEQLIIQAPNSSVHIYEPYPSGADVPFGPPLN 437

  Fly   438 REFLTKVLDLQEGIKEINA---NGT-QLKDICYAPLSDDGSEIDVSQCVVQSIWGYFGDDRERLD 498
            :|.|.:|||||..|:.|.|   |.| .|:|||.||||...     ..|.:.|:..||.:....||
  Rat   438 KEILHQVLDLQIAIESITASYNNETVTLQDICVAPLSPYN-----KNCTIMSVLNYFQNSHAVLD 497

  Fly   499 DHDEDNGFNVTYLD----ALYDCISNPYL----------CLAPYGGPVDPAIALGGFLPPGDQLT 549
            :...|:.|  .|.|    .|| |:..|..          ||..:||||.|.:.|||:   .||
  Rat   498 NQVGDDFF--VYADYHTHFLY-CVRAPASLNDTSLLHGPCLGTFGGPVFPWLVLGGY---DDQ-- 554

  Fly   550 GSTKFELANAIILTFLVKNHHNKTD-LENALTWEKKFVEFMTNYTKNNMSQYMDIAFTSERSIED 613
               .:..|.|:::||.|.|::|.|: |:.|..|||:|:.|:.||...|::    |:||:||||||
  Rat   555 ---NYNNATALVITFPVNNYYNDTEKLQRAWAWEKEFINFVKNYKNPNLT----ISFTAERSIED 612

  Fly   614 ELNRESQSDVLTILVSYLIMFMYIAISLGHVKEFKRVFIDSKITLGIGGVIIVLASVVSSVGVFG 678
            ||||||.|||.|:|:||.:||:||:::|||::.:.||.:||||:|||.|::|||:||..|:|:|.
  Rat   613 ELNRESNSDVFTVLISYAVMFLYISLALGHIQSWNRVLVDSKISLGIAGILIVLSSVTCSLGIFS 677

  Fly   679 YIGLPATLIIVEVIPFLVLAVGVDNIFILVQTHQRDQRKPNETLEQQVGRILGKVGPSMLLTSLS 743
            |||:|.|||::|||||||||||||||||||||:|||:|...|||:||:|||||:|.|:|.|:|.|
  Rat   678 YIGMPLTLIVIEVIPFLVLAVGVDNIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSSFS 742

  Fly   744 ESFCFFLGGLSDMPAVRAFALYAGVALIIDFLLQITCFVSLFTLDTKRREENRMDICCFIKGKKP 808
            |:..||.|.||.||||..|:|:||:|::||||||||||||...||.||:|:||:||.|.::|  |
  Rat   743 ETSAFFFGALSSMPAVHTFSLFAGMAVLIDFLLQITCFVSFLGLDIKRQEKNRLDILCCVRG--P 805

  Fly   809 D---SITSNEGLLYKFFSSVYVPFLMKKIVRASVMVIFFAWLCFSIAIAPRIDIGLDQELAMPQD 870
            |   ...::|..|::||.:.:.|||:...:|..||.:|...|.||||:..:::|||||.|:||.|
  Rat   806 DDGQESQASESYLFRFFKNAFAPFLLTDWLRPVVMAVFVGILSFSIAVVNKVEIGLDQSLSMPND 870

  Fly   871 SFVLHYFQSLNENLNIGPPVYFVLKGDLAYTNSSDQNLVCAGQYCNDDSVLTQIYLASRHSNQTY 935
            |:|:.||:||.:.|:.||||||||:....|::...||:||.|..|::||::.||:.|:.....|.
  Rat   871 SYVIDYFKSLGQYLHSGPPVYFVLEEGYNYSSRRGQNMVCGGMGCDNDSLVQQIFNAAELDTYTR 935

  Fly   936 IARPASSWIDDYFDWAAAASSCCKYRKDSGDFCPHQ--DTSCLRCN-ITKNSLLRPEEKEFVKYL 997
            :....||||||||||.:..||||:....:..||...  |.:|:||. :|.....||:.|||:|:|
  Rat   936 VGFAPSSWIDDYFDWVSPQSSCCRLYNVTHQFCNASVIDPTCVRCRPLTPEGKQRPQGKEFMKFL 1000

  Fly   998 PFFLKDNPDDTCAKAGHAAYGGAVRYSNSHERLNIEASYFMAYHTILKSSADYFLALESARKISA 1062
            |.||.|||:..|.|.|||||..||....  :...|.|:|||.||||||:||||..||:.||.|::
  Rat  1001 PMFLSDNPNPKCGKGGHAAYSSAVNIMG--DDTYIGATYFMTYHTILKTSADYIDALKKARLIAS 1063

  Fly  1063 NITQMLQGRLMSNGVPMASALTVEVFPYSVFYVFYEQYLTMWSDTLQSMGISVLSIFVVTFVLMG 1127
            |||:.::.:          .....||||||||||||||||:..|.:.::.:|:.|||:||.|.:|
  Rat  1064 NITETMRSK----------GSDYRVFPYSVFYVFYEQYLTIIDDAIFNLSVSLGSIFLVTLVALG 1118

  Fly  1128 FDVHSALVVVITITMIVVNLGGLMYYWNISLNAVSLVNLVMAVGISVEFCSHLVHSFATSKSVSQ 1192
            .::.||:::.:||.||:||:.|:|:.|.|||||||||||||..|||||||||:..:|..|...|:
  Rat  1119 CELWSAVIMCLTIAMILVNMFGVMWLWGISLNAVSLVNLVMTCGISVEFCSHITRAFTMSTKGSR 1183

  Fly  1193 IDRAADSLSKMGSSIFSGITLTKFAGILVLAFAKSQIFQVFYFRMYLGIVVIGAAHGLIFLPVLL 1257
            :.||.::|:.||||:|||||||||.||:||||||||||::|||||||.:|::||.||||||||||
  Rat  1184 VSRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLL 1248

  Fly  1258 SYIGAPVSNARLRYHSQ 1274
            ||||..|:.|: |:.:|
  Rat  1249 SYIGPSVNKAK-RHTTQ 1264

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Npc1a	NP_001188769.1	NPC1_N	31..274	CDD:293023	83/255 (33%)
		2A060601	33..1261	CDD:273337	570/1278 (45%)
		Sterol-sensing	651..804	CDD:289145	101/152 (66%)
Npc1	NP_705888.2	NPC1_N	24..268	CDD:293023	83/255 (33%)
		2A060601	26..1252	CDD:273337	570/1278 (45%)
		Sterol-sensing	650..803	CDD:289145	101/152 (66%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166353286
Domainoid	1	1.000	236	1.000	Domainoid score	I2270
eggNOG	1	0.900	-	-		E1_KOG1933
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H228
Inparanoid	1	1.050	1053	1.000	Inparanoid score	I242
OMA	1	1.010	-	-		QHG55095
OrthoDB	1	1.010	-	-		D31089at33208
OrthoFinder	1	1.000	-	-		FOG0001659
OrthoInspector	1	1.000	-	-		oto97593
orthoMCL	1	0.900	-	-		OOG6_101370
Panther	1	1.100	-	-	LDO	PTHR45727
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X943
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	16	15.770