Protein Alignment ath and DHX9

DIOPT Version :10

• Sequence 1: NP_609356.2
• Gene: ath / 34357
• FlyBaseID: FBgn0032194
• Length: 694
• Species: Drosophila melanogaster
• Sequence 2: NP_001348.2
• Gene: DHX9 / 1660
• HGNCID: 2750
• Length: 1270
• Species: Homo sapiens
• Alignment Length: 775
• Identity: 207/775 - (26%)
• Similarity: 340/775 - (43%)
• Gaps: 159/775 - (20%)

  Fly     8 TSRNDAVGP----SPVKFSFKRKYDAMANSPILEKKPHVAQIKQTIAPILLTSKRTSIEQQQKSL 68
            ||.|...||    :|.:.|...|.:.|..   || :.|..|               :|.|:::.|
Human   343 TSSNIDEGPLAFATPEQISMDLKNELMYQ---LE-QDHDLQ---------------AILQERELL 388

  Fly    69 PVFNCRHRILKELEANDTVLIMSETGSGKTTQIPQFLLLAGYAKNGM-----IGITQPRRVAAIT 128
            ||......||:.:..|..|:|...||.|||||:||| :|..:.:|..     |.:|||||::|::
Human   389 PVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQF-ILDDFIQNDRAAECNIVVTQPRRISAVS 452

  Fly   129 VARRVAQELNGTIGDTVGYTVRFEDVTSRA-TKIRFLTDGVLLRESIKDRLLLKYSVIILDEAHE 192
            ||.|||.|.....|.:.||:||||.:..|. ..|.|.|.|||||:  .:..:...|.:|:||.||
Human   453 VAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRK--LEAGIRGISHVIVDEIHE 515

  Fly   193 RTVNADLLFGIVKDAQKERRKQKLANLKVVVTSATMDIDHFGNY-FNCKGMYLEGRTYPV----- 251
            |.:|.|.|..:::|.     .|....:::|:.|||:|...|..| |||..:.:.||||||
Human   516 RDINTDFLLVVLRDV-----VQAYPEVRIVLMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFL 575

  Fly   252 ----RVMH---------TKEEHED----------------------------------------- 262
                ::.|         .|::.:|
Human   576 EDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEKETPFELIEAL 640

  Fly   263 --YIHTVLVTLFHIHRTTPKNHDVLIFLTGQEEIESLAQQIRQLAKIDTTGTTDLRVFTLYAQLS 325
              ||.|:.|.           ..||:||.|...|.::.:.:......   |:...::..|::|:.
Human   641 LKYIETLNVP-----------GAVLVFLPGWNLIYTMQKHLEMNPHF---GSHRYQILPLHSQIP 691

  Fly   326 QGKQLECFVPTPANVRKVILATNIAETSITIPGIRCVIDCGFVKEKSFNTVDGLDVLKSVRISKA 390
            :.:|.:.|.|.|..|.||||:||||||||||..:..|||....|.|.|...:.:....:|..||.
Human   692 REEQRKVFDPVPVGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKT 756

  Fly   391 QAWQRAGRAGRDADGTCYRAYTKAEMDSFADATQPEILRT--NPTSMVLQLLALDIDCNNF--DF 451
            ...||.|||||...|.|:...::|..:.......||:.||  :..::.::||.|. ....|  ..
Human   757 NLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRLG-GIGQFLAKA 820

  Fly   452 LDPPLEDGLRSAYKSLDALGAIKTGDDSYITPLGRQMVQYPLDPKYSKLLLTASSFGCMEEILSL 516
            ::||..|.:..|..:|..|.|:...|:  :|||||.:.:.|::|::.|:::....|...:.|.::
Human   821 IEPPPLDAVIEAEHTLRELDALDANDE--LTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAICTI 883

  Fly   517 VSVLSSDHVFVSNSEKNEMAALAHAKFQ-SKHGDHLTLLNVFNGF----LKSEKPKM-WCHDNYL 575
            .:.......|::..::   ....|..|. ::..||:.||:||..:    :..|:.:: :|....|
Human   884 AAATCFPEPFINEGKR---LGYIHRNFAGNRFSDHVALLSVFQAWDDARMGGEEAEIRFCEHKRL 945

  Fly   576 NLRSLTYARNVRRQLREISEHLHLALNS-----------------SDDIEMLKKCILNGFFENIA 623
            |:.:|......:.||:||      .:||                 .::::::...:..|.:.|:.
Human   946 NMATLRMTWEAKVQLKEI------LINSGFPEDCLLTQVFTNTGPDNNLDVVISLLAFGVYPNVC 1004

  Fly   624 VLQRDGFYITASGNIRSKIHPSSV------LHGKYKPSYILFTEIVQTEQTFLRQVTEIS 677
            ..:.....:|..|. .:.||.|||      ...||...:.:|.|.::|.....:.:|.::
Human  1005 YHKEKRKILTTEGR-NALIHKSSVNCPFSSQDMKYPSPFFVFGEKIRTRAISAKGMTLVT 1063

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ath	NP_609356.2	HrpA	67..>686	CDD:441249	192/712 (27%)
DHX9	NP_001348.2	Interaction with CREBBP. /evidence=ECO:0000269|PubMed:9323138	1..250
		DSRM_DHX9_rpt1	3..71	CDD:380683
		siRNA-binding. /evidence=ECO:0000269|PubMed:23361462, ECO:0007744|PDB:3VYY	5..9
		siRNA-binding. /evidence=ECO:0000269|PubMed:23361462, ECO:0007744|PDB:3VYY	53..55
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	83..118
		DSRM_DHX9_rpt2	178..252	CDD:380684
		siRNA-binding. /evidence=ECO:0000269|PubMed:23361462, ECO:0007744|PDB:3VYX	182..186
		Interaction with BRCA1. /evidence=ECO:0000269|PubMed:9662397	230..325
		siRNA-binding. /evidence=ECO:0000305|PubMed:23361462	234..236
		Necessary for interaction with RNA polymerase II holoenzyme. /evidence=ECO:0000269|PubMed:9323138	255..664		104/358 (29%)
		Necessary for interaction with H2AX. /evidence=ECO:0000269|PubMed:15613478	313..952		186/655 (28%)
		DEXHc_DHX9	330..563	CDD:350730	86/246 (35%)
		MTAD. /evidence=ECO:0000269|PubMed:11416126	331..380		13/55 (24%)
		HrpA	388..>886	CDD:441249	157/522 (30%)
		Core helicase. /evidence=ECO:0000305|PubMed:25062910	398..809		132/432 (31%)
		DEIH box	511..514		2/2 (100%)
		Nuclear localization signal (NLS1). /evidence=ECO:0000255	586..595		0/8 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	588..608		2/19 (11%)
		HA2. /evidence=ECO:0000269|PubMed:25062910	831..919		20/92 (22%)
		OB-fold. /evidence=ECO:0000269|PubMed:20696886, ECO:0000269|PubMed:25062910, ECO:0000269|PubMed:25149208	958..1074		21/113 (19%)
		OB_NTP_bind	994..1073	CDD:400182	15/71 (21%)
		RGG. /evidence=ECO:0000269|PubMed:10207077, ECO:0000269|PubMed:25062910, ECO:0000269|PubMed:25149208, ECO:0000269|PubMed:9111062	1150..1270
		Nuclear localization signal (NLS2). /evidence=ECO:0000269|PubMed:16375861	1155..1173
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1229..1248