DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Toll-4 and TLR9

DIOPT Version :9

Sequence 1:	NP_523519.2	Gene:	Toll-4 / 34235	FlyBaseID:	FBgn0032095	Length:	1125	Species:	Drosophila melanogaster
Sequence 2:	NP_059138.1	Gene:	TLR9 / 54106	HGNCID:	15633	Length:	1032	Species:	Homo sapiens

Alignment Length:	1000	Identity:	224/1000 - (22%)
Similarity:	343/1000 - (34%)	Gaps:	338/1000 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   356 PYKSHCKFTLGINE-VACPL--KCNCSYNRDKSQLEIDCWQKNLTTIPSLPVPKKGSSALVFQSN 417
            |....|..|:..:. :|.|.  :.|.|||             |:.|:|:|  ||...|..:..:|
Human   105 PMHFPCHMTIEPSTFLAVPTLEELNLSYN-------------NIMTVPAL--PKSLISLSLSHTN 154

  Fly   418 LLAELPDNSLEGYH--------------------------------NLKSLDVSYNQLTSLSVSQ 450
            :|. |...||.|.|                                ||..|.:.||.||.:. ..
Human   155 ILM-LDSASLAGLHALRFLFMDGNCYYKNPCRQALEVAPGALLGLGNLTHLSLKYNNLTVVP-RN 217

  Fly   451 LPESLHYLDIRHNKITTLSPQVVEYLYSVNVFNQYGNKWSIYCD--------------EYHLQEF 501
            ||.||.||.:.:|:|..|:|:.:..|.::.|.:..||  ...||              :.|...|
Human   218 LPSSLEYLLLSYNRIVKLAPEDLANLTALRVLDVGGN--CRRCDHAPNPCMECPRHFPQLHPDTF 280

  Fly   502 ----------------FWYKAKLLR-------------------IKTSKFQTIMEYIELS----- 526
                            .|..|...|                   .||..||.:.:..:|:
Human   281 SHLSRLEGLVLKDSSLSWLNASWFRGLGNLRVLDLSENFLYKCITKTKAFQGLTQLRKLNLSFNY 345

  Fly   527 -------------SKGSFV-------ENFFVQNIDQ--------------LYLEAN-----EDEI 552
                         |.||.|       ...|.:::|:              |.|:.|     :..|
Human   346 QKRVSFAHLSLAPSFGSLVALKELDMHGIFFRSLDETTLRPLARLPMLQTLRLQMNFINQAQLGI 410

  Fly   553 IDAFGPSDKYFNL---------KLMEALNHA-----IWLFSGEFDEIILHHLNSPCPYRCSCCFE 603
            ..|| |..:|.:|         :|...:..|     :||..|:.         :|.|....
Human   411 FRAF-PGLRYVDLSDNRISGASELTATMGEADGGEKVWLQPGDL---------APAPVDTP---- 461

  Fly   604 WHTGEFLINCRNLSLDIYPRLPNSIPYKTTLYLDRNEIRKLTNTESLVVAGHASIHKLHMSQNLL 668
             .:.:|..||..|:.              ||.|.||   .|...:..:.|..:.:..|.:|.|.:
Human   462 -SSEDFRPNCSTLNF--------------TLDLSRN---NLVTVQPEMFAQLSHLQCLRLSHNCI 508

  Fly   669 RE-------LPLHLLPENITYLDVRNNLLKYLDDGVIAFLEYRENITKIELSGNPWECNCKAK-- 724
            .:       |||    ..:..||:.:|.|....:.  :|.|. ..:..::||.|......:..
Human   509 SQAVNGSQFLPL----TGLQVLDLSHNKLDLYHEH--SFTEL-PRLEALDLSYNSQPFGMQGVGH 566

  Fly   725 --AFLSFLR--RHEPMEYETVLRRVEITDDKCPEDCICCVDTSNSDSLAYVVDCSGKELSEIPQL 785
              :|::.||  ||..:.:..:  ..:::...|            |.||. .:|.||..|..: ..
Human   567 NFSFVAHLRTLRHLSLAHNNI--HSQVSQQLC------------STSLR-ALDFSGNALGHM-WA 615

  Fly   786 PTPTY--------GQTTLVFERNSLKK-WPSSL--LPGYSSVTRF---YLAHNRLSDIDQLPDKL 836
            ....|        |...|...:|.|.. .|.:|  ||....|.|.   |||..:...:..|| ||
Human   616 EGDLYLHFFQGLSGLIWLDLSQNRLHTLLPQTLRNLPKSLQVLRLRDNYLAFFKWWSLHFLP-KL 679

  Fly   837 EYLDISNNNFSALDD-------RVR--------------GFLQKRMNSSQLQLSL---------- 870
            |.||::.|...||.:       |:|              ||..|.....:|.||.
Human   680 EVLDLAGNQLKALTNGSLPAGTRLRRLDVSCNSISFVAPGFFSKAKELRELNLSANALKTVDHSW 744

  Fly   871 FG-------------NPWTCRC--EDKDFLVFVKEQAKNIANASAIQCIDTGR---SLIEVEETD 917
            ||             ||..|.|  ...|||:.|:.....:  .|.::|...|:   ..|..::..
Human   745 FGPLASALQILDVSANPLHCACGAAFMDFLLEVQAAVPGL--PSRVKCGSPGQLQGLSIFAQDLR 807

  Fly   918 ICPSVLIYYTSLAVSLLIIALSINVFICFRQPIM-------IWFYEHEICLSLAARR----ELDE 971
            :|....:.:...|:|||.:||.:.|      |::       :|:..| :||:....|    ..||
Human   808 LCLDEALSWDCFALSLLAVALGLGV------PMLHHLCGWDLWYCFH-LCLAWLPWRGRQSGRDE 865

  Fly   972 DK-KYDAFLSFTHKDEDLIEEFV-----DRLE--NGRHKFRLCFYLRDWLVGESIPDCINQSVKG 1028
            |. .||||:.| .|.:..:.::|     .:||  .||...|||...||||.|:::.:.:..||.|
Human   866 DALPYDAFVVF-DKTQSAVADWVYNELRGQLEECRGRWALRLCLEERDWLPGKTLFENLWASVYG 929

  Fly  1029 SRRIIILMTKNFLKSTWGRLEFRLALHATSRDRCKRLI-VVLYPDVEHFDDLDSELRAYMVLNTY 1092
            ||:.:.::......|...|..|.||......||...:: |:|.|        |.....|:.|...
Human   930 SRKTLFVLAHTDRVSGLLRASFLLAQQRLLEDRKDVVVLVILSP--------DGRRSRYVRLRQR 986

  Fly  1093 LDRNNPNFWNKLMYSMPHASHLKRS 1117
            |.|.:...|       ||....:||
Human   987 LCRQSVLLW-------PHQPSGQRS 1004

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Toll-4	NP_523519.2	leucine-rich repeat	264..287	CDD:275380
		leucine-rich repeat	288..308	CDD:275380
		leucine-rich repeat	309..334	CDD:275380
		leucine-rich repeat	335..386	CDD:275380	9/32 (28%)
		leucine-rich repeat	388..408	CDD:275380	6/19 (32%)
		LRR_8	412..465	CDD:290566	21/84 (25%)
		leucine-rich repeat	412..432	CDD:275378	7/51 (14%)
		LRR_4	432..470	CDD:289563	16/37 (43%)
		leucine-rich repeat	433..454	CDD:275378	8/20 (40%)
		leucine-rich repeat	455..478	CDD:275378	8/22 (36%)
		leucine-rich repeat	479..490	CDD:275378	3/10 (30%)
		leucine-rich repeat	632..657	CDD:275378	7/24 (29%)
		leucine-rich repeat	658..679	CDD:275378	6/27 (22%)
		leucine-rich repeat	680..706	CDD:275378	6/25 (24%)
		leucine-rich repeat	707..719	CDD:275378	3/11 (27%)
		leucine-rich repeat	771..791	CDD:275380	5/27 (19%)
		leucine-rich repeat	793..815	CDD:275378	7/24 (29%)
		leucine-rich repeat	816..835	CDD:275378	7/21 (33%)
		leucine-rich repeat	836..859	CDD:275378	11/43 (26%)
		TIR	974..1110	CDD:214587	40/143 (28%)
TLR9	NP_059138.1	leucine-rich repeat	43..64	CDD:275380
		Interaction with CpG-DNA. /evidence=ECO:0000250\|UniProtKB:Q2EEY0	47..51
		LRR 1	62..85
		LRR_8	64..135	CDD:290566	9/42 (21%)
		leucine-rich repeat	65..88	CDD:275380
		Interaction with CpG-DNA. /evidence=ECO:0000250\|UniProtKB:Q2EEY0	72..77
		LRR 2	87..110		1/4 (25%)
		leucine-rich repeat	89..124	CDD:275380	4/18 (22%)
		Interaction with CpG-DNA. /evidence=ECO:0000250\|UniProtKB:Q2EEY0	95..109		1/3 (33%)
		LRR 3	122..147		11/39 (28%)
		LRR_8	123..177	CDD:290566	19/69 (28%)
		leucine-rich repeat	125..144	CDD:275380	9/33 (27%)
		leucine-rich repeat	145..168	CDD:275380	7/23 (30%)
		LRR 4	150..166		5/16 (31%)
		LRR 5	167..190		1/22 (5%)
		leucine-rich repeat	169..200	CDD:275380	0/30 (0%)
		Interaction with CpG-DNA. /evidence=ECO:0000250\|UniProtKB:Q2EEY0	179..181		0/1 (0%)
		LRR 6	198..221		9/23 (39%)
		LRR_8	200..254	CDD:290566	19/54 (35%)
		leucine-rich repeat	201..221	CDD:275380	8/20 (40%)
		leucine-rich repeat	222..245	CDD:275380	8/22 (36%)
		LRR 7	223..242		6/18 (33%)
		LRR 8	243..268		6/26 (23%)
		leucine-rich repeat	246..285	CDD:275380	7/40 (18%)
		LRR 9	283..306		2/22 (9%)
		LRR_8	284..344	CDD:290566	8/59 (14%)
		leucine-rich repeat	286..307	CDD:275380	3/20 (15%)
		LRR 10	308..332		3/23 (13%)
		leucine-rich repeat	310..335	CDD:275380	4/24 (17%)
		LRR 11	333..356		1/22 (5%)
		leucine-rich repeat	336..365	CDD:275380	4/28 (14%)
		LRR 12	363..386		3/22 (14%)
		leucine-rich repeat	366..392	CDD:275380	2/25 (8%)
		LRR 13	390..413		4/22 (18%)
		leucine-rich repeat	393..416	CDD:275380	6/23 (26%)
		LRR 14	415..440		4/24 (17%)
		leucine-rich repeat	417..472	CDD:275380	12/68 (18%)
		LRR 15	470..494		8/40 (20%)
		LRR_RI	476..739	CDD:238064	67/289 (23%)
		leucine-rich repeat	476..497	CDD:275380	7/23 (30%)
		LRR_8	496..555	CDD:290566	14/65 (22%)
		LRR 16	496..519		3/22 (14%)
		leucine-rich repeat	498..522	CDD:275380	6/27 (22%)
		LRR 17	520..543		6/28 (21%)
		leucine-rich repeat	523..546	CDD:275380	6/25 (24%)
		LRR 18	545..572		4/26 (15%)
		leucine-rich repeat	547..599	CDD:275380	10/65 (15%)
		LRR 19	574..598		5/37 (14%)
		LRR_8	598..665	CDD:290566	17/68 (25%)
		leucine-rich repeat	600..629	CDD:275380	6/30 (20%)
		LRR 20	600..622		6/23 (26%)
		LRR 21	627..650		6/22 (27%)
		leucine-rich repeat	630..654	CDD:275380	7/23 (30%)
		LRR 22	652..675		6/22 (27%)
		leucine-rich repeat	655..678	CDD:275380	7/23 (30%)
		LRR 23	676..699		10/23 (43%)
		leucine-rich repeat	679..702	CDD:275380	7/22 (32%)
		LRR_8	701..762	CDD:290566	11/60 (18%)
		LRR 24	701..723		4/21 (19%)
		leucine-rich repeat	703..726	CDD:275380	4/22 (18%)
		LRR 25	724..747		4/22 (18%)
		leucine-rich repeat	727..748	CDD:275380	5/20 (25%)
		LRR 26	749..772		4/22 (18%)
		leucine-rich repeat	752..770	CDD:275380	4/17 (24%)
TIR_2	870..1009	CDD:304906	43/150 (29%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG4641
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D147327at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.820