DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Toll-4 and 18w

DIOPT Version :9

Sequence 1:	NP_523519.2	Gene:	Toll-4 / 34235	FlyBaseID:	FBgn0032095	Length:	1125	Species:	Drosophila melanogaster
Sequence 2:	NP_476814.1	Gene:	18w / 37277	FlyBaseID:	FBgn0004364	Length:	1385	Species:	Drosophila melanogaster

Alignment Length:	1189	Identity:	251/1189 - (21%)
Similarity:	427/1189 - (35%)	Gaps:	428/1189 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly   257 IFHGKNKMSALIFNGLKIKGLTNNTFENLTSLNTLIFDNVFLKDLSFLRSSTLQSSLTYCIMKVD 321
            :|....|:|.|..:..|::.:..|.||.|.||..|                ||:|         .
  Fly    85 LFRQLQKLSELRIDACKLQRVPPNAFEGLMSLKRL----------------TLES---------H 124

  Fly   322 NMV--DLKSFEKFTNLEIIEVSQYKGFKNFTAFICEPYKSHCKFTLGINEVACPLKCNCSYNRDK 384
            |.|  ..|:.|       :....::|.|..:           :..||.|.:
  Fly   125 NAVWGPGKTLE-------LHGQSFQGLKELS-----------ELHLGDNNI-------------- 157

  Fly   385 SQLEIDCWQKNLTTIPSLPVPK------KGSSALVFQSNLLA----ELPDNSLEGYHNLKSLDVS 439
            .||....|    .::|||.:..      :.:..|.|...|.|    ...:.::.|...|::||||
  Fly   158 RQLPEGVW----CSMPSLQLLNLTQNRIRSAEFLGFSEKLCAGSALSNANGAVSGGSELQTLDVS 218

  Fly   440 YNQLTSL----SVSQLPESLHYLDIRHNKITTLSPQVVEYLYSVNVFN-QYGNKWSIYCD----- 494
            :|:|.||    ..|:| ..|..|.::||.|:||:|..:..|.|:.|.| .|.:..|:..:
  Fly   219 FNELRSLPDAWGASRL-RRLQTLSLQHNNISTLAPNALAGLSSLRVLNISYNHLVSLPSEAFAGN 282

  Fly   495 ----EYHLQEFFWY---KAKLLRIK------------------TSKFQTIMEYIELS-------- 526
                |.|||....|   |..|.|::                  .|.|..::..|.|:
  Fly   283 KELRELHLQGNDLYELPKGLLHRLEQLLVLDLSGNQLTSHHVDNSTFAGLIRLIVLNLSNNALTR 347

  Fly   527 --SKGSFVENFFVQNIDQLYLEANEDEIID--AFGPSDKYFNLKLMEALNHAI--WLFSGEF--D 583
              || :|.|.:|:|.:|   :..|....|:  ||.|......|.|.|...|.:  .:|:|.:  .
  Fly   348 IGSK-TFKELYFLQILD---MRNNSIGHIEEGAFLPLYNLHTLNLAENRLHTLDNRIFNGLYVLT 408

  Fly   584 EIILHH-----LNSPCPYRCSCCFEWH-------------------------------------- 605
            ::.|::     :.|.....||...|..
  Fly   409 KLTLNNNLVSIVESQAFRNCSDLKELDLSSNQLTEVPEAVQDLSMLKTLDLGENQISEFKNNTFR 473

  Fly   606 -----TGEFLINCR--NLSLDIYPRLPNSIPYKTTLYLDRNEIRKL------TNTE--------- 648
                 ||..||:.|  |:::.::..||.    .:.|.|.:|.|:.:      .|||
  Fly   474 NLNQLTGLRLIDNRIGNITVGMFQDLPR----LSVLNLAKNRIQSIERGAFDKNTEIEAIRLDKN 534

  Fly   649 -----SLVVAGHASIHKLHMSQNLLRELPLHLLPENITYLDVRNNLLK----------------- 691
                 :.:.|..||:..|::|:|.|.......:|.|:.:||:..|.::
  Fly   535 FLTDINGIFATLASLLWLNLSENHLVWFDYAFIPSNLKWLDIHGNYIEALGNYYKLQEEIRVTTL 599

  Fly   692 ----------------------YLDDGVIAFLE---------------YRENITKIELS------ 713
                                  ::::.:|..::               |...::||.|:
  Fly   600 DASHNRITEIGAMSVPNSIELLFINNNIIGQIQANTFVDKTRLARVDLYANVLSKISLNALRVAP 664

  Fly   714 -------------GNPWECNC-------------------------------------------K 722
                         |||:||:|                                           .
  Fly   665 VSAEKPVPEFYLGGNPFECDCSMEWLQRINNLTTRQHPHVVDLGNIECLMPHSRSAPLRPLASLS 729

  Fly   723 AKAFLSFLRRHEP-----MEYETVLRRVEITDDKCPEDCICCVDTSNSDSLAYVVDCSGKELSEI 782
            |..|:.....|.|     .|||..  ..|:.   ||.:|.|..|.:.:.:   :|||..::|:.:
  Fly   730 ASDFVCKYESHCPPTCHCCEYEQC--ECEVI---CPGNCSCFHDATWATN---IVDCGRQDLAAL 786

  Fly   783 P-QLPTPTYGQTTLVFERNSLKKWPSSLLPGYSSVTRFYLAHNRL-----SDIDQLPDKLEYLDI 841
            | ::|...   :.|..:.|::.:.....|.|..::...||..:.|     ..:.||.: |..|.:
  Fly   787 PNRIPQDV---SDLYLDGNNMPELEVGHLTGRRNLRALYLNASNLMTLQNGSLAQLVN-LRVLHL 847

  Fly   842 SNNNFSALDD---RVRGFLQK-------------------------RMNSSQLQ----------- 867
            .||..:||:.   |..|.|::                         |:::::|.
  Fly   848 ENNKLTALEGTEFRSLGLLRELYLHNNMLTHISNATFEPLVSLEVLRLDNNRLSSLPHLQYRHSL 912

  Fly   868 --LSLFGNPWTCRCED-KDFLVFVKEQAKNIANASAIQCIDTG-----------------RSLIE 912
              |:|..|.|:|||:. ::...||.:.|..:.:|..|.|:|.|                 ..|::
  Fly   913 QGLTLGRNAWSCRCQQLRELAQFVSDNAMVVRDAHDIYCLDAGIKRELELIGNLANGPDCSDLLD 977

  Fly   913 VEETDICPSV-------LIYYTSLAVSLLIIALSINVFICFRQPIMIWFYEH---EICLSLAARR 967
            ...::|..|.       |....::.|.:.::.:.|.||: ||:.:.:|.:.|   .:|    ..|
  Fly   978 ASASNISSSQDLAGGYRLPLLAAVLVLIFLVVVLIIVFV-FRESVRMWLFAHYGVRVC----EPR 1037

  Fly   968 ELDEDKKYDAFLSFTHKDEDLIEEFVDR-----LENGRHKFRLCFYLRDWLVGESIPDCINQSVK 1027
            ..|..|.|||.:..:.||    .|||.|     ||:||..||||...|| |..::....:.:..:
  Fly  1038 FEDAGKLYDAIILHSEKD----YEFVCRNIAAELEHGRPPFRLCIQQRD-LPPQASHLQLVEGAR 1097

  Fly  1028 GSRRIIILMTKNFLKSTWGRLEFRLALHATSRDRCKRLIVVLYPDVEHFDDLDSELRAYM--VLN 1090
            .||:||:::|:|.|.:.|.|:|||.|.|.:.|...::|:::....|....:..:||..|:  |.:
  Fly  1098 ASRKIILVLTRNLLATEWNRIEFRNAFHESLRGLAQKLVIIEETSVSAEAEDVAELSPYLKSVPS 1162

  Fly  1091 TYLDRNNPNFWNKLMYSMP 1109
            ..|...:..||.||.|::|
  Fly  1163 NRLLTCDRYFWEKLRYAIP 1181

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Toll-4	NP_523519.2	leucine-rich repeat	264..287	CDD:275380	7/22 (32%)
		leucine-rich repeat	288..308	CDD:275380	2/19 (11%)
		leucine-rich repeat	309..334	CDD:275380	6/26 (23%)
		leucine-rich repeat	335..386	CDD:275380	5/50 (10%)
		leucine-rich repeat	388..408	CDD:275380	4/25 (16%)
		LRR_8	412..465	CDD:290566	20/60 (33%)
		leucine-rich repeat	412..432	CDD:275378	5/23 (22%)
		LRR_4	432..470	CDD:289563	18/41 (44%)
		leucine-rich repeat	433..454	CDD:275378	11/24 (46%)
		leucine-rich repeat	455..478	CDD:275378	9/22 (41%)
		leucine-rich repeat	479..490	CDD:275378	3/11 (27%)
		leucine-rich repeat	632..657	CDD:275378	8/44 (18%)
		leucine-rich repeat	658..679	CDD:275378	5/20 (25%)
		leucine-rich repeat	680..706	CDD:275378	5/79 (6%)
		leucine-rich repeat	707..719	CDD:275378	6/30 (20%)
		leucine-rich repeat	771..791	CDD:275380	6/20 (30%)
		leucine-rich repeat	793..815	CDD:275378	4/21 (19%)
		leucine-rich repeat	816..835	CDD:275378	5/23 (22%)
		leucine-rich repeat	836..859	CDD:275378	9/25 (36%)
		TIR	974..1110	CDD:214587	48/143 (34%)
18w	NP_476814.1	leucine-rich repeat	66..91	CDD:275380	1/5 (20%)
		leucine-rich repeat	92..115	CDD:275380	7/22 (32%)
		LRR_8	115..181	CDD:290566	21/126 (17%)
		leucine-rich repeat	116..146	CDD:275380	10/61 (16%)
		leucine-rich repeat	147..170	CDD:275380	6/51 (12%)
		leucine-rich repeat	171..201	CDD:275380	5/29 (17%)
		LRR_RI	210..464	CDD:238064	60/258 (23%)
		leucine-rich repeat	212..236	CDD:275380	11/24 (46%)
		LRR_8	235..295	CDD:290566	18/59 (31%)
		leucine-rich repeat	237..260	CDD:275380	9/22 (41%)
		leucine-rich repeat	261..284	CDD:275380	4/22 (18%)
		LRR_8	283..345	CDD:290566	12/61 (20%)
		leucine-rich repeat	285..308	CDD:275380	8/22 (36%)
		leucine-rich repeat	309..334	CDD:275380	2/24 (8%)
		LRR_8	334..393	CDD:290566	17/62 (27%)
		leucine-rich repeat	335..358	CDD:275380	6/23 (26%)
		leucine-rich repeat	359..382	CDD:275380	7/25 (28%)
		LRR_8	382..441	CDD:290566	11/58 (19%)
		leucine-rich repeat	383..406	CDD:275380	6/22 (27%)
		leucine-rich repeat	407..430	CDD:275380	3/22 (14%)
		LRR_8	431..488	CDD:290566	5/56 (9%)
		leucine-rich repeat	431..451	CDD:275380	1/19 (5%)
		leucine-rich repeat	454..477	CDD:275380	0/22 (0%)
		LRR_8	477..559	CDD:290566	21/85 (25%)
		leucine-rich repeat	478..499	CDD:275380	6/20 (30%)
		leucine-rich repeat	502..525	CDD:275380	5/22 (23%)
		leucine-rich repeat	526..548	CDD:275380	1/21 (5%)
		leucine-rich repeat	590..617	CDD:275380	0/26 (0%)
		leucine-rich repeat	618..641	CDD:275380	1/22 (5%)
		leucine-rich repeat	642..689	CDD:275380	10/46 (22%)
		LRRCT	679..736	CDD:214507	7/56 (13%)
		leucine-rich repeat	775..796	CDD:275380	6/23 (26%)
		LRR_8	797..852	CDD:290566	13/55 (24%)
		leucine-rich repeat	797..817	CDD:275380	4/19 (21%)
		leucine-rich repeat	818..841	CDD:275380	5/22 (23%)
		LRR_8	841..900	CDD:290566	10/59 (17%)
		leucine-rich repeat	842..865	CDD:275380	7/22 (32%)
		LRR_4	866..907	CDD:289563	3/40 (8%)
		leucine-rich repeat	866..889	CDD:275380	1/22 (5%)
		leucine-rich repeat	890..911	CDD:275380	2/20 (10%)
		TIR	1045..1183	CDD:214587	48/142 (34%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45468844
Domainoid	1	1.000	44	1.000	Domainoid score	I4673
eggNOG	1	0.900	-	-		E2759_KOG4641
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D147327at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X221
	6	5.750