DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Sema1a and Sema4b

DIOPT Version :9

Sequence 1:	NP_001260265.1	Gene:	Sema1a / 34192	FlyBaseID:	FBgn0011259	Length:	1131	Species:	Drosophila melanogaster
Sequence 2:	NP_001347064.1	Gene:	Sema4b / 20352	MGIID:	107559	Length:	823	Species:	Mus musculus

Alignment Length:	1022	Identity:	258/1022 - (25%)
Similarity:	388/1022 - (37%)	Gaps:	331/1022 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    54 LLLTVLVIGNQSAWQENIRPKLYVELGPED--VLKF----VGNESVVDHFKLVTKDGNSLLIGAR 112
            |||.:|::..::.....:.|::.|.||.|:  :.||    :.|.:.:    |:::||.:|.:|||
Mouse    14 LLLLLLLLLLRTTTTRALGPRISVPLGSEERLIRKFEAENISNYTAL----LLSQDGKTLYVGAR 74

  Fly   113 NTVF----NLSIHDLVEQQRLVWTSPEDDTKMCLVKGKD-EEACQNYIRIMVVPSPGRLFVCGTN 172
            ..:|    |||.....|.|.|:|::..|..:.|..|||| :..|||||:|::..:...|..|||.
Mouse    75 EALFALNSNLSFLPGGEYQELLWSADADRKQQCSFKGKDPKRDCQNYIKILLPLNSSHLLTCGTA 139

  Fly   173 SFRPMCNTYIISDSNYTLEATKNGQAV-------CPYDPRHNSTSVLADNELYSGTVADFSGSDP 230
            :|.|:| .| |..:::||...:.|..:       ||:||...||:::.|.|||:|||:.|.|:||
Mouse   140 AFSPLC-AY-IHIASFTLAQDEAGNVILEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDP 202

  Fly   231 IIYR----EPLQTEQYDSLS-LNAPNFVSS----------FTQGDFVYFFFRETAVEFINCGKAI 280
            .|.|    .|.:||  .||: |..|.||:|          ....|.:||||.||..||......|
Mouse   203 AISRSQSSRPTKTE--SSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTI 265

  Fly   281 YSRVARVCKWDKGGPHRFRNRWTSFLKSRLNCSIPGDYPFYFNEIQSASNLVEGQYGSMSSKLIY 345
            .||||||||.|:||....:.|||||||::|.||.|.| .|.||.:|....|..... .....|.|
Mouse   266 VSRVARVCKGDEGGERVLQQRWTSFLKAQLLCSRPDD-GFPFNVLQDVFTLNPNPQ-DWRKTLFY 328

  Fly   346 GVFNTP--SNSIPGSAVCAFALQDIADTFEGQFKEQTGINSNWLPVNNAKVPDPRPGSC------ 402
            |||.:.  ..:..|||:|.|.:.|:...|:|.:|:.......|. ....:||.||||:|
Mouse   329 GVFTSQWHRGTTEGSAICVFTMNDVQKAFDGLYKKVNRETQQWY-TETHQVPTPRPGACITNSAR 392

  Fly   403 ---HNDSRALPDPTLNFIKTHSLMDENVPAFFSQPILVRTSTIYRFTQIAVDAQIKTPG-GKTYD 463
               .|.|..|||..|||:|.|.|||..|.   |:.:|::...  |:.::||.   :.|| ..|||
Mouse   393 ERKINSSLQLPDRVLNFLKDHFLMDGQVR---SRLLLLQPRA--RYQRVAVH---RVPGLHSTYD 449

  Fly   464 VIFVGTDHGKIIKSVNAESADSADKVTSVVIEEIDVLTKSEPIRNLEIVRTMQYDQPKDGSYDDG 528
            |:|:||..|::.|:|...|...       :|||:.:..:.:|::||.:            ....|
Mouse   450 VLFLGTGDGRLHKAVTLSSRVH-------IIEELQIFPQGQPVQNLLL------------DSHGG 495

  Fly   529 KLIIVTDSQVVAIQLHRCHNDKITSCSECVALQDPYCAWDKIAGKCRSHGAPRWLEENYFYQNVA 593
            .|...:.|.||.:.:..|  ....:|.:|:..:||||||
Mouse   496 LLYASSHSGVVQVPVANC--SLYPTCGDCLLARDPYCAW-------------------------- 532

  Fly   594 TGQHAACPSGKINSKDANAGEQKGFRNDMDLLDSRRQSKDQEIIDNIDKNFEGPQISADIINAQY 658
            ||  :||....:...|               |.||..::|          .||..:.....|:.|
Mouse   533 TG--SACRLASLYQPD---------------LASRPWTQD----------IEGASVKELCKNSSY 570

  Fly   659 TVETLVMAVLAGSIFSLLVGFFTGYFCGRRCHKDEDDNLPYPDTEYEYFEQRQNVNSFPSSCRIQ 723
            ....||.                    |:.|.                              ::|
Mouse   571 KARFLVP--------------------GKPCK------------------------------QVQ 585

  Fly   724 QEPKLLPQVEEVTYAEPVLLPQPPPPNKMHSPKNTLRKPPMHQM------HQG------------ 770
            .:|..:                           |||..|.:..:      |.|
Mouse   586 IQPNTV---------------------------NTLACPLLSNLATRLWVHNGAPVNASASCRVL 623

  Fly   771 PNSETLFQFQPDGYNTQQSYRGRDNFGTLRSHQVMGDNYRRGDGFSTTRSVKKAVNNTNTRNRSL 835
            |..:.|......|....|.:...:.|..|.:
Mouse   624 PTGDLLLVGSQQGLGVFQCWSIEEGFQQLVA---------------------------------- 654

  Fly   836 GRARRQPPRHGIVTQHRSNSPQQQQQQSQQPHSSSGSSPVMSNSS--SSPAPPSSSPSPQESPKN 898
                             |..|:..::......:....:||:.|:|  |:||...:|....:|..|
Mouse   655 -----------------SYCPEVMEEGVMDQKNQRDGTPVIINTSRVSAPAGGRASWGADKSYWN 702

  Fly   899 -----CS------------YIYRDXLICNTKSMPLIQAQSTHAQPHPQSHPHPLPPP-------G 939
                 |:            ::||     :...|.|...|...|..||::.|..|||.       |
Mouse   703 EFLVMCTLFVFAMVLLFLFFLYR-----HRDGMKLFLKQGECASVHPKTRPIVLPPETRPLNGVG 762

  Fly   940 PTTPPAQPRARSPMIG-----RTYAKS-----------MPVTPVQPQ 970
            |.:.|...|....:..     |.:.:|           :.|:||.|:
Mouse   763 PPSTPLDHRGYQALSDSSPGPRVFTESEKRPLSIQDSFVEVSPVCPR 809

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sema1a	NP_001260265.1	Sema_1A	91..546	CDD:200498	171/493 (35%)
Sema1a	NP_001260265.1	PSI	545..>579	CDD:214655	9/33 (27%)
Sema4b	NP_001347064.1	Sema	48..511	CDD:417757	174/500 (35%)
		PSI	512..558	CDD:396154	18/100 (18%)
		Ig_Sema4B_like	579..663	CDD:409456	17/191 (9%)
		Ig strand B	592..596	CDD:409456	3/3 (100%)
		Ig strand C	604..608	CDD:409456	0/3 (0%)
		Ig strand E	626..629	CDD:409456	0/2 (0%)
		Ig strand F	639..644	CDD:409456	1/4 (25%)
		Ig strand G	656..659	CDD:409456	0/2 (0%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3611
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR11036
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.910