Protein Alignment Uba4 and uba3

DIOPT Version :10

• Sequence 1: NP_609240.2
• Gene: Uba4 / 34187
• FlyBaseID: FBgn0032054
• Length: 453
• Species: Drosophila melanogaster
• Sequence 2: NP_998632.1
• Gene: uba3 / 406776
• ZFINID: ZDB-GENE-040426-2825
• Length: 462
• Species: Danio rerio
• Alignment Length: 412
• Identity: 92/412 - (22%)
• Similarity: 137/412 - (33%)
• Gaps: 121/412 - (29%)

  Fly    79 PDFGVQG---QLKLKNSSVLIVGLGGLGCPAAQYLAAAGCGHLGLVDYDEVERSNFHRQILHSED 140
            |||....   |..|....:|::|.|||||...:.||.:|..|:.:||.|.::.||.:||.|....
Zfish    53 PDFEASTESLQFLLDTCKILVIGAGGLGCELLKDLALSGFRHIHVVDMDTIDVSNLNRQFLFRPK 117

  Fly   141 RCGMSKAESARIALLELNPHCEIQCHSRMLYPHNAMHIIRGYDVVLDCTDNVPTR-----YLLS- 199
            ..|..|||.|...:.:..|.|.:..|.:.:...:.. ..|.:.:|:...|:|..|     .|||
Zfish   118 DVGRPKAEVAADFVNDRVPGCSVVPHFKKIQDLDET-FYRQFHIVVCGLDSVIARRWMNGMLLSL 181

  Fly   200 ----DACVMLSK--PLVSGSALKMDGQLTVYNYANGPCYRCIFPVPPP------------PEAVT 246
                |..:..|.  ||:.|......|...|.......|..|...:.||            |....
Zfish   182 LIYEDGVLDPSSIIPLIDGGTEGFKGNARVILPGMTACIDCTLELYPPQINFPMCTIASMPRLPE 246

  Fly   247 NC---------------GDGGVLGA---------------------VTG---------------- 259
            :|               |||.||..                     :||
Zfish   247 HCVEYVRMLLWPKEKPFGDGVVLDGDDPKHIQWVYQKSLERAAEFNITGVTYRLTQGVVKRIIPA 311

  Fly   260 ------TIGAMQALEAIKVIVGMGDVLAGRLLIFDGGSGVFRNIRIRSKRPNCHMCSAQP----- 313
                  .|.|..|.|..|:... ..|.....|:|:...|::.......::.||..||..|
Zfish   312 VASTNAVIAAACATEVFKIATS-AYVPLNNYLVFNDVDGLYTYTFEAERKENCSACSQVPQDMQF 375

  Fly   314 -----------LITELINYEMFCGMHAT--DKNNPMLLLSTDERLSVEDYQQKIQAQPHL----- 360
                       .:||..:.:|......|  |..|..|.|.|  ..|:|:     :.:|:|
Zfish   376 TPSAKLQEVLDYLTENASLQMKSPAITTTLDGKNKTLYLQT--VASIEE-----RTRPNLSKTLK 433

  Fly   361 ---LIDVRPTAEFEICQLPEAV 379
               |:|.:..|..:: ..|:.|
Zfish   434 ELGLVDGQELAVADV-TTPQTV 454

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Uba4	NP_609240.2	ThiF	65..311	CDD:440244	72/316 (23%)
		RHOD_ThiF	336..453	CDD:238784	12/52 (23%)
uba3	NP_998632.1	Interaction with ube2m N-terminus. /evidence=ECO:0000250	52..69		5/15 (33%)
		Uba3_RUB	70..367	CDD:238765	66/298 (22%)
		Interaction with ube2m N-terminus. /evidence=ECO:0000250	156..160		1/3 (33%)
		Interaction with ube2m N-terminus. /evidence=ECO:0000250	191..216		6/24 (25%)
		Interaction with nedd8. /evidence=ECO:0000250	226..228		0/1 (0%)
		Interaction with nae1. /evidence=ECO:0000250	241..247		1/5 (20%)
		Interaction with nae1. /evidence=ECO:0000250	291..294		0/2 (0%)
		Interaction with ube2m N-terminus. /evidence=ECO:0000250	330..337		0/7 (0%)
		Interaction with nedd8. /evidence=ECO:0000250	351..356		0/4 (0%)
		Interaction with ube2m core domain. /evidence=ECO:0000250	367..462		20/96 (21%)
		E2_bind	377..460	CDD:462611	18/86 (21%)