DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment AlaRS and ALA1

DIOPT Version :9

Sequence 1:	NP_523511.2	Gene:	AlaRS / 34156	FlyBaseID:	FBgn0027094	Length:	966	Species:	Drosophila melanogaster
Sequence 2:	NP_014980.3	Gene:	ALA1 / 854513	SGDID:	S000005862	Length:	958	Species:	Saccharomyces cerevisiae

Alignment Length:	975	Identity:	483/975 - (49%)
Similarity:	628/975 - (64%)	Gaps:	40/975 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 TAKEVRNAYLDFFKEQKHIYVHSSSTIPLDDPTLLFANAGMNQFKPIFLGTADPNSEMSKWVRVA 69
            ||..|||.:||:||.::|.:|.||..:|.||||||||||||||:|||||||.||.|:.....|..
Yeast    10 TATNVRNTFLDYFKSKEHKFVKSSPVVPFDDPTLLFANAGMNQYKPIFLGTVDPASDFYTLKRAY 74

  Fly    70 NTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICSWAWEFLTQRLALPKDRLYV 134
            |:|||||||||||||:|||||.|||||||||||||||||||||..:::|..||:...:|||||||
Yeast    75 NSQKCIRAGGKHNDLEDVGKDSYHHTFFEMLGNWSFGDYFKKEAITYSWTLLTEVYGIPKDRLYV 139

  Fly   135 TYFGGDAASGLEPDLECKQMWLDLGLKPEHILPGSMKDNFWEMGETGPCGPCSELHFDRIGGRSV 199
            |||.||...|||||.|.:::|.::|:..:|||||:.||||||||:.||||||||:|:||||||:.
Yeast   140 TYFEGDEKLGLEPDTEARELWKNVGVPDDHILPGNAKDNFWEMGDQGPCGPCSEIHYDRIGGRNA 204

  Fly   200 PELVNMDDPDVLEIWNLVFIQYNRESDGSLKQLPKKHIDCGMGFERLVSVIQNKRSNYDTDLFVP 264
            ..|||||||||||:|||||||:|||.|||||.||.||||.||||||||||:|:.|||||||:|.|
Yeast   205 ASLVNMDDPDVLEVWNLVFIQFNREQDGSLKPLPAKHIDTGMGFERLVSVLQDVRSNYDTDVFTP 269

  Fly   265 LFDAIQAGTGAPPYQGRVGADDVDGIDMAYRVLADHARTITIALADGGTPDNTGRGYVLRRILRR 329
            ||:.||..|...||.|..|.:|.||||.||||||||.||:|.||||||.|:|.||||||||||||
Yeast   270 LFERIQEITSVRPYSGNFGENDKDGIDTAYRVLADHVRTLTFALADGGVPNNEGRGYVLRRILRR 334

  Fly   330 AVRYATEKLNAKPG-FFATLVNTVVDLLGDAFPEVKKDPQHIIDIINEEELQFLKTLTRGRNLLN 393
            ..|||.:.:|...| ||:||..|::..:.|.|||:.|||..:.:|::|||..|.|||.||..|..
Yeast   335 GARYARKYMNYPIGNFFSTLAPTLISQVQDIFPELAKDPAFLFEILDEEEASFAKTLDRGERLFE 399

  Fly   394 R--TIEKLGNQTTIPGDVAWRLYDTYGFPVDLTQLMAEEKSLKIDMDGYEAAKHNSYVLSQGKGA 456
            :  :........|:.|...||||||||||||||:|||||:.||||..|:|.||..||..|: :|.
Yeast   400 KYASAASKTESKTLDGKQVWRLYDTYGFPVDLTELMAEEQGLKIDGPGFEKAKQESYEASK-RGG 463

  Fly   457 SKIEE--INLDVHAISQLQEQGVPPTNDTFKYKYEAVSDERDSAYNYGVCN--SKIVALRFENQF 517
            .|.:.  |.|:||.:|:|.:..||.|||.||               ||..|  ..|:.|.....|
Yeast   464 KKDQSDLIKLNVHELSELNDAKVPKTNDEFK---------------YGSANVEGTILKLHDGTNF 513

  Fly   518 VNEITS-GQKAGIVLDKTNFYAESGGQIYDQGALVKVNDEANEFLVDRVYNRGGYILHIG-VVEG 580
            |:|||. |:|.||:||||.||||.|||.||.|.:  |.|:|.||.|:.|....|::.|.| :.||
Yeast   514 VDEITEPGKKYGIILDKTCFYAEQGGQEYDTGKI--VIDDAAEFNVENVQLYNGFVFHTGSLEEG 576

  Fly   581 TLKVGDELELHIDVERRWLTMKNHSATHALNHCLLQVLGKDTEQKGSLVVPEKLRFDFNSKAAMT 645
            .|.|||::....|..||:....||:.||.||..|.:.||.|.:||||||.||||||||:.|.|::
Yeast   577 KLSVGDKIIASFDELRRFPIKNNHTGTHILNFALKETLGNDVDQKGSLVAPEKLRFDFSHKKAVS 641

  Fly   646 IEQVSKTEQLTKEMVYKNVPIYAKESKLALAKKIRGLRSVFDEVYPDPVRVISFGVPVDELEQNP 710
            .|::.|.|.:..|.:.:|:.::.||..|.|||.|.|:|:||.|.|||||||:|.|.|::||..||
Yeast   642 NEELKKVEDICNEQIKENLQVFYKEIPLDLAKSIDGVRAVFGETYPDPVRVVSVGKPIEELLANP 706

  Fly   711 DSEAGEQTSVEFCGGTHLRRSGHIMDFVISSEEAIAKGIRRIVALTGPEALKALKKSEAFEQEIV 775
            .:|...:.|:|||||||:.::|.|..|||..|..||||||||||:||.||.:|.:.:|.|
Yeast   707 ANEEWTKYSIEFCGGTHVNKTGDIKYFVILEESGIAKGIRRIVAVTGTEAFEAQRLAEQF----- 766

  Fly   776 RLKATIDNDKSGKDSKSHVKE--IVELTEQISHATIPYVKKDEMRNLLKGLKKTLDDKERALRAA 838
                ..|.|.:.|...|.:||  :.||..::...:|..:.|:|::.....::|.:.|:.::....
Yeast   767 ----AADLDAADKLPFSPIKEKKLKELGVKLGQLSISVITKNELKQKFNKIEKAVKDEVKSRAKK 827

  Fly   839 VSVTVVERAKTLCEANPNATVLVEQLEAFNNTKALDAALKQVRS--QLPDAAAMFLSVDADSKKI 901
            .:...::..||..|.|.||..||:.::...|.||:..|:..::|  .:.|.:...|:.:....::
Yeast   828 ENKQTLDEVKTFFETNENAPYLVKFIDISPNAKAITEAINYMKSNDSVKDKSIYLLAGNDPEGRV 892

  Fly   902 FCLSSVPKSAVEKGLKANEWVQHVSATLGGKGGGKPESAQASGTNYEKVDEIVQLASKFAQSKLS 966
            .....:..:|:.||:..:...:.||:.:|||.|||....|..|.....:.:.|.......:.|||
Yeast   893 AHGCYISNAALAKGIDGSALAKKVSSIIGGKAGGKGNVFQGMGDKPAAIKDAVDDLESLFKEKLS 957

Fly 967 966
Yeast 958 957

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
AlaRS	NP_523511.2	PLN02900	5..963	CDD:215487	480/970 (49%)
		AlaRS_core	6..253	CDD:238360	172/246 (70%)
		tRNA_SAD	529..>626	CDD:298782	45/97 (46%)
		tRNA_SAD	694..752	CDD:285247	31/57 (54%)
		DHHA1	888..957	CDD:280440	15/68 (22%)
ALA1	NP_014980.3	PLN02900	7..946	CDD:215487	479/962 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157344254
Domainoid	1	1.000	673	1.000	Domainoid score	I14
eggNOG	1	0.900	-	-		E1_COG0013
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H1213
Inparanoid	1	1.050	907	1.000	Inparanoid score	I41
Isobase	1	0.950	-	0	Normalized mean entropy	S431
OMA	1	1.010	-	-		QHG60684
OrthoFinder	1	1.000	-	-		FOG0001295
OrthoInspector	1	1.000	-	-		oto100131
orthoMCL	1	0.900	-	-		OOG6_100414
Panther	1	1.100	-	-	LDO	PTHR11777
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1429
SonicParanoid	1	1.000	-	-		X999
TreeFam	1	0.960	-	-
	16	15.740