DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG7627 and Cftr

DIOPT Version :9

Sequence 1:	NP_001245943.1	Gene:	CG7627 / 34148	FlyBaseID:	FBgn0032026	Length:	1355	Species:	Drosophila melanogaster
Sequence 2:	NP_066388.1	Gene:	Cftr / 12638	MGIID:	88388	Length:	1476	Species:	Mus musculus

Alignment Length:	1475	Identity:	422/1475 - (28%)
Similarity:	723/1475 - (49%)	Gaps:	189/1475 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 LPENPREHCNFISAACFWYTMPTFIKGRKRTLDTKDLYRALKEHKSETLGNKLCASWELELEKTK 73
            :.::|.|..:|||...|.:|.|...||.:..|:..|:|:|.....::.|..||...|:.| :.:|
Mouse     1 MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSADSADHLSEKLEREWDRE-QASK 64

  Fly    74 GKPNLLRALLRVFGWYFALLGLVLFLLELGLRTLQPIFLLKLIAYYTHGSESIES-AYYYAAGVI 137
            ..|.|:.||.|.|.|.|...|::|:|.|: .:.:||:.|.::||.|...::...| |.|...|:.
Mouse    65 KNPQLIHALRRCFFWRFLFYGILLYLGEV-TKAVQPVLLGRIIASYDPENKVERSIAIYLGIGLC 128

  Fly   138 LCSALNVIIMHPYMLGTMHVGLKMRVGMCSMIYRKALRLSKSALGDTTAGHVVNLMSNDVGRLDL 202
            |...:..:::||.:.|...:|::||..|.|:||:|.|:||...|...:.|.:|:|:||::.:.|.
Mouse   129 LLFIVRTLLLHPAIFGLHRIGMQMRTAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDE 193

  Fly   203 ATIFVHYLWVGPLETLFITYLMYREIGIAAVFGVAFMLLFIPLQAYLGKRTSVLRLRTALRTDER 267
            .....|::|:.||:...:..|::..:..:|..|:..:::.:..||.|||.....|.:.|.:.:||
Mouse   194 GLALAHFIWIAPLQVTLLMGLLWDLLQFSAFCGLGLLIILVIFQAILGKMMVKYRDQRAAKINER 258

  Fly   268 VRMMNEIISGIQVIKMYAWELPFEHMVAFARKKEINAIRHVSYIRGILLSFIIFLTRVSIFLSLV 332
            :.:.:|||..|..:|.|.||...|.|:...|:.|:...|..:|:|....|...|.....:|||::
Mouse   259 LVITSEIIDNIYSVKAYCWESAMEKMIENLREVELKMTRKAAYMRFFTSSAFFFSGFFVVFLSVL 323

  Fly   333 GYVLLGTFLTPEVAFLITAYYNILRTTMTVFFPQGISQMAETLVSIKRVQKYMQSDETNVMDMSV 397
            .|.::...:..:: |...::..:||.::|..||..:....::...|:::|.::|..|..|::.::
Mouse   324 PYTVINGIVLRKI-FTTISFCIVLRMSVTRQFPTAVQIWYDSFGMIRKIQDFLQKQEYKVLEYNL 387

  Fly   398 DLTEDFQGSNQETVHADGDEERDEAEDKLLGPPIATVNENAKLS--EAGISISGLMAKWDVNSPD 460
            ..|    |...|.|.|..:|...|..:|     :...|.:.|.|  |..:|.|.|..   |.:|
Mouse   388 MTT----GIIMENVTAFWEEGFGELLEK-----VQQSNGDRKHSSDENNVSFSHLCL---VGNP- 439

  Fly   461 YSLNGVNLRVQPGTMLGIVGRTGSGKSSLIQAILGELPAESGEIKVNGSMSYASQEPWLFSGTVR 525
             .|..:||.::.|.||.|.|.|||||:||:..|||||.|..|.||.:|.:|:.||..|:..||::
Mouse   440 -VLKNINLNIEKGEMLAITGSTGSGKTSLLMLILGELEASEGIIKHSGRVSFCSQFSWIMPGTIK 503

  Fly   526 QNILFGQPMDRRRYAKVVKKCALERDFELLPFKDKTIVGERGASLSGGQKARISLARAVYRETSI 590
            :||:||...|..||..|||.|.|::|......:|.|::||.|.:|||||:||||||||||::..:
Mouse   504 ENIIFGVSYDEYRYKSVVKACQLQQDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDADL 568

  Fly   591 YLLDDPLSAVDTHVARHLFEQCMRGYLRERIVILATHQLQFLQHADQIVIMDKGRVSAVGTYESL 655
            ||||.|...:|......:||.|:...:..:..||.|.:::.|:.||:|:|:.:|.....||:..|
Mouse   569 YLLDSPFGYLDVFTEEQVFESCVCKLMANKTRILVTSKMEHLRKADKILILHQGSSYFYGTFSEL 633

  Fly   656 RESGLDFASMLADPERDEQSEERSRS-----------------------RSGSYTHSHSDQRRNS 697
            :....||:|.|...:..:|..|..||                       :|...|....::|:||
Mouse   634 QSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDSSAPWSKPKQSFRQTGEVGEKRKNS 698

  Fly   698 ---------EQSLLSMADSCM----DDLEAEQANNQERQEAGQIGLRLYSKYFKAGGGF------ 743
                     :.|::.....|:    |||:.::.:.....|.|:..|. .|.....|..|
Mouse   699 ILNSFSSVRKISIVQKTPLCIDGESDDLQEKRLSLVPDSEQGEAALP-RSNMIATGPTFPGRRRQ 762

  Fly   744 -----FAFFVMMGFCVLSQGLASLGDYFLSYWVT-KKGNVAYRADNNDTT-------RSEELE-- 793
                 ..|....|...|.:...|:....|...:: .:.:|..|..:.|:|       ..|:|:
Mouse   763 SVLDLMTFTPNSGSSNLQRTRTSIRKISLVPQISLNEVDVYSRRLSQDSTLNITEEINEEDLKEC 827

  Fly   794 --------PRLSTWLRDIGLSVDAEMLDTYI--FT---------VITVLTILVTVARS------- 832
                    |.::||             :||:  ||         :..||..||.||.|
Mouse   828 FLDDVIKIPPVTTW-------------NTYLRYFTLHKGLLLVLIWCVLVFLVEVAASLFVLWLL 879

  Fly   833 -----------------------------FLFF---------------------NLAMKASIRLH 847
                                         ::|:                     :..:.||..||
Mouse   880 KNNPVNSGNNGTKISNSSYVVIITSTSFYYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILH 944

  Fly   848 NSMFRGITRAAMYFFNTNPSGRILNRFSKDMGQVDEILPAVMMDVIQIFLALAGIVIVIAVVNPL 912
            ..|...|..|.|...:...:|.||||||||:..:|:.||..:.|.||:...:.|.:||::.:.|.
Mouse   945 RKMLHSILHAPMSTISKLKAGGILNRFSKDIAILDDFLPLTIFDFIQLVFIVIGAIIVVSALQPY 1009

  Fly   913 FLIPTVVLGIIFYQLRTFYLKTSRDVKRMEAITRSPVYSHLAASLTGLSTIRAFGAQRVLEAEFD 977
            ..:.||...::|..||.::|.|::.:|::|:..|||:::||..||.||.|:|||..|...|..|.
Mouse  1010 IFLATVPGLVVFILLRAYFLHTAQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFH 1074

  Fly   978 NYQDMHSSAFYMFISTSRAFGYWLDCFCVIYIAIITLSFFIFPPANGGDVGLAITQAMGMTGMVQ 1042
            ...::|::.::|:::|.|.|...:|...|::..::|....:......|..|:.:|.||.:...:|
Mouse  1075 KALNLHTANWFMYLATLRWFQMRIDMIFVLFFIVVTFISILTTGEGEGTAGIILTLAMNIMSTLQ 1139

  Fly  1043 WGMRQSAELENTMTAVERVVEYEDIEPEGALEAPADKKPPKS------------------WPEQG 1089
            |.:..|.:.::.|.:|.||.::.||:.|.::.....|:.|:.                  ||..|
Mouse  1140 WAVNSSIDTDSLMRSVSRVFKFIDIQTEESMYTQIIKELPREGSSDVLVIKNEHVKKSDIWPSGG 1204

  Fly  1090 KIVFDELSLRYTPDPKSENVLKSLSFVIKPKEKVGIVGRTGAGKSSLINALFRLSYNDGSVLIDK 1154
            ::|..:|:::|..|..:  ||:::||.|.|.::||::||||:|||:|::|..|:....|.:.||.
Mouse  1205 EMVVKDLTVKYMDDGNA--VLENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDG 1267

  Fly  1155 RDTSEMGLHDLRSKISIIPQEPVLFSGTMRYNLDPFDEYSDDKLWRSLEEVKLKEVVADLPSGLQ 1219
            ...:.:.|.:.|....:|.|:..:||||.|.||||..::.|:::|:..:||.||.|:...|..|.
Mouse  1268 VSWNSVTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLN 1332

  Fly  1220 SKITEGGTNFSVGQRQLVCLARAILRENRILVMDEATANVDPQTDGLIQTTIRNKFKECTVLTIA 1284
            ..:.:||...|.|.:||:||||::|.:.:|:::||.:|::||.|..:|:..::..|..|||:...
Mouse  1333 FTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCE 1397

  Fly  1285 HRLHTIMDSDKVLVMDAGRAVEFGTPYELLTLADSKVFHGMVKQT 1329
            ||:..::|..:.||::.....::.:...|  |::..:|...:..:
Mouse  1398 HRIEAMLDCQRFLVIEESNVWQYDSLQAL--LSEKSIFQQAISSS 1440

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG7627	NP_001245943.1	CFTR_protein	12..1315	CDD:273530	420/1456 (29%)
		ABC_membrane	107..360	CDD:294371	72/253 (28%)
		ABCC_MRP_domain1	445..645	CDD:213217	86/199 (43%)
		ABC_membrane	815..1014	CDD:294371	70/266 (26%)
		ABCC_MRP_domain2	1089..1310	CDD:213211	76/220 (35%)
Cftr	NP_066388.1	CFTR_protein	1..1464	CDD:273530	422/1475 (29%)
		ABC_membrane	85..303	CDD:294371	66/218 (30%)
		ABCC_CFTR1	389..670	CDD:213258	110/294 (37%)
		CFTR_R	639..844	CDD:291094	38/218 (17%)
		Disordered R region. /evidence=ECO:0000250\|UniProtKB:P13569	654..826		30/172 (17%)
		ABC_membrane	861..1142	CDD:279056	75/280 (27%)
		ABCC_CFTR2	1204..1464	CDD:213256	79/241 (33%)
		Interaction with GORASP2. /evidence=ECO:0000250\|UniProtKB:P13569	1382..1476		12/61 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1446..1476
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:P13569	1474..1476
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167841709
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24223
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.850