Protein Alignment Btk and zap70

DIOPT Version :10

• Sequence 1: NP_476745.1
• Gene: Btk / 34132
• FlyBaseID: FBgn0003502
• Length: 786
• Species: Drosophila melanogaster
• Sequence 2: NP_001018425.1
• Gene: zap70 / 553615
• ZFINID: ZDB-GENE-050522-257
• Length: 604
• Species: Danio rerio
• Alignment Length: 602
• Identity: 170/602 - (28%)
• Similarity: 275/602 - (45%)
• Gaps: 126/602 - (20%)

  Fly   279 LGLGGGGGGGGSCTPTSLQPQSSLTTFKQSPTLLNG--------------------NGTLLDANM 323
            |.|.|.|||              |...:|....|.|                    |||...|
Zfish    24 LKLAGMGGG--------------LFLLRQCLRSLGGYVLSMIWNLDFYHYSIEKQLNGTYCIA-- 72

  Fly   324 PGGIPTPGTPN-----SKAKDNSHFVKLVVALYPFKAIEGGDLSLEK----NAEYEVIDDSQEHW 379
             ||.|..|...     ||..|.     ||..|.. ..:...:..:.|    |....::.:...|.
Zfish    73 -GGKPHCGPAELCEYYSKDPDG-----LVCTLRK-PCLRSPETEIRKGVFDNLRDNMLREYVRHT 130

  Fly   380 WKVK-DALGN--VGYIPSNYVKPKALLGLERYEWYVGDMSRQRAESLLKQGDK-EGCFVVRKSST 440
            ||:: ||:..  :...| ...|..|....|:..|:.|.:.||..|..|..|.: :|.|:||:...
Zfish   131 WKLEGDAMEQAIISQAP-QLEKLIATTAHEKMPWFHGKIPRQEGERRLYSGSQPDGKFLVRERDE 194

  Fly   441 KGLYTLSL-HTKVPQSHVKHYHIKQNARCEYYLSEKHCCETIPDLINYHRHNSGGLACRLKS--- 501
            .|.:.||: :.|.    |.||.|.::...:..:.|....:|:..|:.|.:....||...|:.
Zfish   195 MGTFALSVTYGKT----VYHYQILRDKSGKIAMPEGTKFDTVWQLVEYLKMKPDGLVTVLREPCV 255

  Fly   502 -------SPCDRP-------------VP---------PTAGLSHDKWE---------------IH 522
                   :|.:.|             ||         |:..:.||.:.               |.
Zfish   256 NQNNAAPAPANAPRRSRGNGYTPPPMVPKPMGAEGSRPSLPMDHDGFTSPYDDPNELKKKTLFIK 320

  Fly   523 PMELMLME-ELGSGQFGVVRRGKWR---GSIDTAVKMMK-EGTMS-EDDFIEEAKVMTKLQHPNL 581
            ..:||:.| |||||.||.|::|.::   ..||.|:|::| |...| .|:.:.||::|.:|..|.:
Zfish   321 RDKLMIDEVELGSGNFGCVKKGVFKMESKQIDVAIKVLKNENEKSVRDEMMREAEIMHQLSDPFI 385

  Fly   582 VQLYGVCSKHRPIYIVTEYMKHGSLLNYLR-RHEKTLIGNMGLLLDMCIQVSKGMTYLERHNYIH 645
            |::.|:| :...:.:|.|....|.|..:|. :.::....|:.:|:.   |||.||.|||..|::|
Zfish   386 VRMIGLC-EAEALMLVMEMAPAGPLNKFLSGKKDQITTENIVMLMH---QVSMGMKYLEGRNFVH 446

  Fly   646 RDLAARNCLVGSENVVKVADFGLARYV--LDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWA 708
            |||||||.|:.::...|::||||::.:  .|:.|.:..|.|:|:||..||.:::.:||||||||:
Zfish   447 RDLAARNVLLVNQQYAKISDFGLSKALGADDNYYKARTGGKWPLKWYAPECIHFHKFSSKSDVWS 511

  Fly   709 YGVLMWEIFTCGKMPYGRLKNTEVVERVQRGIILEKPKSCAKEIYDVMKLCWSHGPEERPAFRVL 773
            :|:.|||.|:.|..||.::|..||:..::.|..|:.|.:|.:.:|::||.||::..|||..|..:
Zfish   512 FGITMWEAFSFGGKPYKKMKGPEVITYIEGGSRLDCPAACPEAMYELMKECWTYKHEERLNFAKV 576

  Fly   774 MDQLAL----VAQTLTD 786
            .:::..    :|:.:.|
Zfish   577 EEKMRTFYYSIAKKIPD 593

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Btk	NP_476745.1	PH_Btk	44..222	CDD:269944
		SH3_Tec_like	346..399	CDD:212702	11/59 (19%)
		SH2_Tec_family	403..505	CDD:198188	28/113 (25%)
		PTKc_Tec_like	521..778	CDD:173637	100/265 (38%)
zap70	NP_001018425.1	SH2_N-SH2_Zap70_Syk_like	8..111	CDD:198191	24/109 (22%)
		SH2_C-SH2_Zap70	152..256	CDD:198265	28/107 (26%)
		PTKc_Zap-70	320..588	CDD:270686	99/271 (37%)