Protein Alignment Piezo and PIEZO2

DIOPT Version :9

• Sequence 1: NP_001303314.1
• Gene: Piezo / 34112
• FlyBaseID: FBgn0264953
• Length: 2973
• Species: Drosophila melanogaster
• Sequence 2: NP_001365112.1
• Gene: PIEZO2 / 63895
• HGNCID: 26270
• Length: 2865
• Species: Homo sapiens
• Alignment Length: 3272
• Identity: 877/3272 - (26%)
• Similarity: 1386/3272 - (42%)
• Gaps: 739/3272 - (22%)

  Fly     1 MVFSYACMVLQRIVVPAVLVLAALMRPVGISFVYLLMFFVSPFVPLATRRNFKGSVTAFFIILLT 65
            |.....|.::.|:::|..|.:|...|..|:|||||:...:.|.....|:...:|........|..
Human     1 MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKTTMQGHTGRLLKSLCF 65

  Fly    66 LSTLVLLGHITLQILAVSL-----TLPIYNCSFSERLLRHIGFVSFIDLQPFAIIEWLVPEVLVF 125
            :|...||.||...|..|||     ..|.||||..|:..|.|||.|.........|...||::.:|
Human    66 ISLSFLLLHIIFHITLVSLEAQHRIAPGYNCSTWEKTFRQIGFESLKGADAGNGIRVFVPDIGMF 130

  Fly   126 ATSLGSYLTVKRVASQPVGAE------QLENGEVVDGQ---AENAQTSSQPSAADANGGD----- 176
            ..||..:|..:.:..:||..|      :.||.|:.:|:   :|.|....:    |.||||
Human   131 IASLTIWLLCRNIVQKPVTDEAAQSNPEFENEELAEGEKIDSEEALIYEE----DFNGGDGVEGE 191

  Fly   177 VQQATVTTPLQQQQQQLRKRVSMISQHIHFEGLVKISPLFCLATLFFAAVLRPSVPGGFYFLIFL 241
            ::::|.....::......|....|...|...|.|.::.|     |..:.::.||:....||.:||
Human   192 LEESTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTIL-----LGSSGMMLPSLTSSVYFFVFL 251

  Fly   242 LSGTYWATCQTLQRGFALLLRCVMVVLVL----HSLSIVSYQTPWMQSHLNHTTLTARLIGLEPL 302
            ...|:|:.|:|..   .||..|:.|:|.:    |.:.:..||..:.|..:......|||.|::.:
Human   252 GLCTWWSWCRTFD---PLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSV 313

  Fly   303 IESYCSPDIRVFLYNNKLSLDSYLNPFALFFAYFALALTTKHLIKPRLVRQSTRK------ARTP 361
            |::.||...:: :.|..||...:.||..|...|:.||...:..::..||:....|      |.:|
Human   314 IQTDCSSTWKI-IVNPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDEGTKEEDKALACSP 377

  Fly   362 QPLESG-----------------------SSVAPS----VTQRGNDMQL---------------- 383
            ..:.:|                       ....||    ||..||.:..
Human   378 IQITAGRRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPGKA 442

  Fly   384 --------------ESMEQRSEQENTTTSILDQIS---------YGFVSVGGFIYQNSYIFTNIL 425
                          ||.|:|.|:|.......::.|         :..|||..||.:.|||...|.
Human   443 DLYSTPQYRWEPSDESSEKREEEEEEKEEFEEERSREEKRSIKVHAMVSVFQFIMKQSYICALIA 507

  Fly   426 MMAWSIVYHSWLTFVLLLSANVLWMIPNQRKAMMRSSPFIVLYAEALLIAQYIYGMDLNNEELP- 489
            ||||||.||||||||||:.:..||||.|:||..|.||||:|:|...|||.|||:..     |||
Human   508 MMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSF-----ELPE 567

  Fly   490 -TSVPTAGINLQQIGF---ERPIENQMRPCVPLIVKTAFVLMFWVTSRQFFKEKR---------- 540
             ..||         ||   :.|.|        |..|..|.:.||:..||...|::
Human   568 IKKVP---------GFLEKKEPGE--------LASKILFTITFWLLLRQHLTEQKALQEKEALLS 615

  Fly   541 --------DRRRDSTLADFIAPLQITVGSAGSSYLINDGKKTSKFLKKA---------------- 581
                    :..:|..|.|      |.|..........:.|:..:..||.
Human   616 EVKIGSQENEEKDEELQD------IQVEGEPKEEEEEEAKEEKQERKKVEQEEAEEEDEQDIMKV 674

  Fly   582 -GDVIKNLLVRLWIWLLVLVIFLCAITGENMTGFRICYMALFLFFLLVFQSSSKAWVKIMYGFWL 645
             |:::..:.::.||::...:.|..:..|: :..::|.||.||||.:.::|...:.|.||:..||:
Human   675 LGNLVVAMFIKYWIYVCGGMFFFVSFEGK-IVMYKIIYMVLFLFCVALYQVHYEWWRKILKYFWM 738

  Fly   646 FLIFYAMSILILIYTYQFDKFDTYWSDYLNVSATLQKDIGLKRYQTKDLFLHLVSPTIIVILTVI 710
            .::.|.|.:||.||||||:.|...|.:...:.....:|:|||::...:||..:..||..:::.::
Human   739 SVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKKEKLEDLGLKQFTVAELFTRIFIPTSFLLVCIL 803

  Fly   711 QVHYFHKRFI------------------------------------ASLQQQPLA--GGS----- 732
            .:||||.||:                                    ..:.|..||  .||
Human   804 HLHYFHDRFLELTDLKSIPSKEDNTIYSHAKVNGRVYLIINSIKKKLPIHQNELAHPEGSLPDLT 868

  Fly   733 ------AQQKPTETTALEPAPSKRRG-----SAGSLRKSQGPSAEAAPGATTDFETSVRDLVRIS 786
                  :.:||......||...|..|     ..|.|.|....|.|.........|......:|..
Human   869 MMHLTASLEKPEVRKLAEPGEEKLEGYSEKAQKGDLGKDSEESEEDGEEEEESEEEEETSDLRNK 933

  Fly   787 FRKIKNKSEYIFKNFKDVF-------WRFLELHIMKAVYIAAFVCSVSEVCVLHIIFV---GFCV 841
            :..:.::...:|..|.:.|       |..|||||:|.|.......||.||.:.:.:|:   .|.:
Human   934 WHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFAL 998

  Fly   842 LGATSRKAVQVVISRLISFIVT----VIVLSKMIYQIEYLSHSQHNVVCS---DNRT-------- 891
            ..|..|        ||.|.:.|    ||::.||:||::.:.....:|.||   :|:|
Human   999 PYAKLR--------RLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNIPFNELN 1055

  Fly   892 --------ANNAEWIGLTKADKVTGGLMSLLRTYIIYMVIVTMHAVISLRQLQMRVKIGALNAPP 948
                    .:..||:||.|:..    |:..||..::.:.|:.....|...|...|.: ..|.||.
Human  1056 KSLLYSAPIDPTEWVGLRKSSP----LLVYLRNNLLMLAILAFEVTIYRHQEYYRGR-NNLTAPV 1115

  Fly   949 TKLLFPNIIRADAEKDLVGLVKYLLNFGFYKFGIEISLIALVSTITYRQDIVAVVYALWLVVLLL 1013
            ::.:|.:|.|...:..|:...||.:|:.|||||:|...:..|:.|..|.|..|:::|.||:.:|.
Human  1116 SRTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLY 1180

  Fly  1014 LRRSQC-AKIWGVFQAFFAISILTQYIVLVGLPPSSCLVFPW--DEGPFGEGIQRWAMLPGALHF 1075
            .||.:. |:||..:..|.|..|..||.:.:|:||:.|..:||  ....|.:.|.:|...|..:..
Human  1181 RRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVR 1245

  Fly  1076 NHVPKLIFDFIVLVILNRQKSIFCIEQRYASNDDYPGGSNRSVI--ADIAQLGRVPFDNPTHDFC 1138
            .:...|::||::|:..:.|:.||..|.:.|..  ...|.|..:.  .|.|...:   .||..||.
Human  1246 PNPVFLVYDFMLLLCASLQRQIFEDENKAAVR--IMAGDNVEICMNLDAASFSQ---HNPVPDFI 1305

  Fly  1139 SYIRNYSDILKNGVLCGFYWFTLAVVFLAGTNIADLLALGYLIGAFIFLWQGSDFYLRPIHTIIF 1203
             :.|:|.|:.|..:....:||.|.::|:.||....:..:|||:..|.||..|.|..|:||.:|:.
Human  1306 -HCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGDLLLKPIKSILR 1369

  Fly  1204 RWKWLLAFNVANILIKTSFQMAGCLFMTQLTKDCCWLVHMLGITCTSNVLTEQIMLPEEAELALK 1268
            .|.||:|:||..|.:|....:..|.::..|..:.|||:....:.||    .:...:|    .|..
Human  1370 YWDWLIAYNVFVITMKNILSIGACGYIGTLVHNSCWLIQAFSLACT----VKGYQMP----AANS 1426

  Fly  1269 PGECPKITHQVVLLWDTICFAFIIFQLRIFKSHYFCHIITDTKANNILASRGADIIESLRHKQIA 1333
            |  |...:.:..::||:|||||::.|.|:|.|:||.|::.|.||:.|||||||::.::...|.:.
Human  1427 P--CTLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVK 1489

  Fly  1334 HRHDHEKQVLHKIKRKMERIRATQQKMLRPLDKQTHFDGRRTPSEHAMSDVAPLVHNSSFTSCQG 1398
            .|.:.||:.:.::||:|:||:|.|||..:..::..                       |.|...|
Human  1490 ARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERML-----------------------SLTQEPG 1531

  Fly  1399 SGYLTPDEHSSATSGTSSPARASILSSSGGSVE----SVDSADAAVIIEPEINVMPCDEITDYVD 1459
            .|     :.....|.......|....:.|...:    .||.|...             ...||..
Human  1532 EG-----QDMQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMV-------------RSGDYYL 1578

  Fly  1460 VQSVSEEETTVAMPKKDLSCQSKECVFKSPEPSKPTTPSTDALSTLLTEGFLEPKRISLGLAPPE 1524
            .::.||||....:.|:|            .||     |...|....:.:           .|...
Human  1579 FETDSEEEEEEELKKED------------EEP-----PRRSAFQRAIGK-----------FASAI 1615

  Fly  1525 LSPPVGMQCLAPPLAAYATAMASSAASSVLSTNLTPNLRREH-------------RRQSSTNAAV 1576
            |:.|..:..|...:..|....|.....:.::...|. ||:.|             ||:.|....|
Human  1616 LALPKSVIKLPKTILQYLIRAAKFVYQAWITDPKTA-LRQRHKEKKRSAREERKRRRKGSKEGPV 1679

  Fly  1577 SWNETVSIKRSPMKKQMSADKEEVVTMRHKSLHRRHQSMGNASYSLDEASQSQRKRLSSNLSGAR 1641
            .|.:.   :..|:||: |...:.::                             ||:.:.|.
Human  1680 EWEDR---EDEPIKKK-SDGPDNII-----------------------------KRIFNILK--- 1708

  Fly  1642 DEAALRSAQRPHSWGPVGTSSYMESLMCAFYEPALLHGPSTRAGDYYMFEEMDDKFELDLIHDEI 1706
                       .:|                                .:|....|.|...|
Human  1709 -----------FTW--------------------------------VLFLATVDSFTTWL----- 1725

  Fly  1707 DFLEEENITESE-MKMQRRKTLYDVWKDLNDAEYRRHFYMRER--SYASEPGSRILLKLDDEKEK 1768
            :.:..|:|..|. ::::|.....::.|.........|.|.:..  :.:.|.|      ||...|.
Human  1726 NSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESG------LDTIDEH 1784

  Fly  1769 IGPDLGLASDHDEDD----DTIECDLGMRTSTLSEPLDFGRRSQTLLEVKSKDAP-TGE--FPST 1826
            .|...|..:.|..|.    |:|       :|..:|......|..||.::..::.| |.|  .|..
Human  1785 PGAASGAQTAHRMDSLDSHDSI-------SSCYTEATMLFSRQSTLDDLDGQEIPKTSERARPRL 1842

  Fly  1827 SKGISKERDAATASSS--ASPAPTRDVGDLPVIPPPSTGLGREQTSKETSDSKSKMEVDSGEVTA 1889
            .|.:|.:..:::|.|.  ||..||:           .|.|...|.:.||.:          ||.|
Human  1843 RKMLSMDMSSSSADSGSLASSEPTQ-----------CTMLYSRQGTTETIE----------EVEA 1886

  Fly  1890 KDSDEDFDTNPIIRLLEGFLVTLTIRLNRFSRNYRFVNRILAGEKKTLKESSSLNRLGLSSAAAM 1954
            :..:|...|.|..|..:.:..|            .:....:..|:.:|.....|.:.
Human  1887 EQEEEAGSTAPEPREAKEYEAT------------GYDVGAMGAEEASLTPEEELTQF-------- 1931

  Fly  1955 FHFLKSNLESDESEPPASSSTPRRVVIAPPNATEHSDPTSTTLNTNTTTTPLSPPEPLQPLQPLQ 2019
                 |.|:.|...||:.|...         :.||....|...:.......:||.:
Human  1932 -----STLDGDVEAPPSYSKAV---------SFEHLSFGSQDDSAGKNRMAVSPDD--------- 1973

  Fly  2020 PNTTSTPQQQHQHIRAAEEIIELPVDTVDGVAHRKQSINSS--PPAKGTMLSRKSDCGLPEIRIK 2082
                                            .|...:.||  ||.
Human  1974 --------------------------------SRTDKLGSSILPPL------------------- 1987

  Fly  2083 APSIERGAHYYHNHHSGGGSGSLSKHWSYEQVDSAGEFNLEEENFAQRDHHIIVEVLISSWY-AL 2146
                        .|........|.|.:..::::.:.:|.:.:..|.         :|..:.| .|
Human  1988 ------------THELTASELLLKKMFHDDELEESEKFYVGQPRFL---------LLFYAMYNTL 2031

  Fly  2147 LANTDLICYIVVFINQVVNASLISLPLPIMVFLWGTLSLPRPTKTFWVTLIAYTQAIVLIKCIFQ 2211
            :|.::::||.|:.:|.:|:||:|:|.|||::|||..||:|||::.||:..|.||:..:::|..||
Human  2032 VARSEMVCYFVIILNHMVSASMITLLLPILIFLWAMLSVPRPSRRFWMMAIVYTEVAIVVKYFFQ 2096

  Fly  2212 FKLI-WS-NYHQLPNQPLTPAKIFGVENKAHYAIYDLILLLVLFLHRYLLKSQGLWKSGYKDTDN 2274
            |... |: |.....::|..|..|.|||.|..|.:||||.||.||.||.:||..|||     |.|:
Human  2097 FGFFPWNKNVEVNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLW-----DEDD 2156

  Fly  2275 QFTKPTA---SIDE-------RDDSDNL-------------------------------SQPDSR 2298
            ......|   |.||       ||.||:|                               |:|..|
Human  2157 MTESGMAREESDDELSLGHGRRDSSDSLKSINLAASVESVHVTFPEQQTAVRRKRSGSSSEPSQR 2221

  Fly  2299 -QLNDDAAQKLSLQVSQASLPGSPEFS--KTGINQLERTKYTSSLYK------------------ 2342
             ..:.:.:|:.|.....:|..||...|  :.|..:|...|....|.|
Human  2222 SSFSSNRSQRGSTSTRNSSQKGSSVLSIKQKGKRELYMEKLQEHLIKAKAFTIKKTLEIYVPIKQ 2286

  Fly  2343 FFFSLVHKSRLA-TDVYALMFLCDFVNFFVLLFGFTAFGTQQTESDEGVQTYLAENKVPIPFLIM 2406
            ||::|:|....| ||||.||||.|.|:|.:::|||.|||.....:|  :.:.|:|::||.|||:|
Human  2287 FFYNLIHPEYSAVTDVYVLMFLADTVDFIIIVFGFWAFGKHSAAAD--ITSSLSEDQVPGPFLVM 2349

  Fly  2407 LLVQFLLIVIDRALYLRKALVNKIIFHFFSVIGIHIWMFFVVPAVTERTFNSLAPPIIFYVIKCF 2471
            :|:||..:|:||||||||.::.|:||....|.|||.||||::|.||||.|:......::|.:||.
Human  2350 VLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCV 2414

  Fly  2472 YMLLSSYQIKSGYPKRILGNFFTKGFSMVNMIAFKVYMQIPFLYELRTILDWVCIDSTMTIFDWL 2536
            |..||:|||:.|||.|:||||.||.::.||:..|:.:..:|||.|||.::|||..|:|:::..|:
Human  2415 YFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWI 2479

  Fly  2537 KMEDIFSNIYLIRCTRQSETDFPAMRAQKKASLSKLIMGGTIVLLIVICIWGPLCLFALGNAV-G 2600
            .:|||:::|::::|.|:||..:|..|.|||..:.|..|||.|::|::..:|.||...:|..:| |
Human  2480 CVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAG 2544

  Fly  2601 TSNVPFHVSLSIRIGPYDPIYTTNNYDSIFEI-NPEMYSQMTNAYIKEKQALTFIAGYDATDVAA 2664
            ..|.|..||::|.:|.|.||:|.:...|..:: :.:.:::...|:.::..|:.|:..|:..|:..
Human  2545 VINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITV 2609

  Fly  2665 VRLAGNSPSLWNIAPPDRQRLLNDLRN-NHTLKARFSYSLTRKAPAKGLKENVGDEHAISLDE-S 2727
            ..|.|||.|||.|:||.:|:::::|.: |.:....||:|:.|..       ::|.:..|:.|: |
Human  2610 AELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNL-------SLGAKSEIATDKLS 2667

  Fly  2728 FEGRAALIHMLSETHDVEPIYSNGTTNGTTPEVEEVVVIPGMIPKFIKVLNSGDAAVVSVLSPKH 2792
            |.     :..::..:..:.|..|.|.:..||     |.|..:.|.::|..:..::..:..|..::
Human  2668 FP-----LKNITRKNIAKMIAGNSTESSKTP-----VTIEKIYPYYVKAPSDSNSKPIKQLLSEN 2722

  Fly  2793 YDYRPLVIKMHRDNET--NGLWWEIRDYCNDTFYNETLSKFAYSNCTSGIVMYTFNDKKFPSTFS 2855
             ::..:.|.:.|||.|  |..|| :.:...:..||..         :..:.:..||||..|.:..
Human  2723 -NFMDITIILSRDNTTKYNSEWW-VLNLTGNRIYNPN---------SQALELVVFNDKVSPPSLG 2776

  Fly  2856 FLTAGGIIGLYTTFVLLASRFMKSFIGGQNRKIMFEDLPYVDRVLQLCLDIYLVREALEFALEED 2920
            ||...||:|||.:.||:..:|::.|..|.:..||||:||.|||:|:||.||:||||..|..||||
Human  2777 FLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEED 2841

  Fly  2921 LFAKLLFLYRSPETLIKWTRPK 2942
            |:|||:||||||||:|||||.|
Human  2842 LYAKLIFLYRSPETMIKWTREK 2863

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Piezo	NP_001303314.1	PIEZO	1226..>1363	CDD:292539	48/136 (35%)
		Piezo_RRas_bdg	2511..2941	CDD:288978	151/435 (35%)
PIEZO2	NP_001365112.1	PIEZO	1391..1658	CDD:406366	80/346 (23%)
		Piezo_RRas_bdg	2453..2862	CDD:403405	151/436 (35%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165149573
Domainoid	1	1.000	485	1.000	Domainoid score	I397
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H49695
Inparanoid	1	1.050	1094	1.000	Inparanoid score	I239
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG46055
OrthoDB	1	1.010	-	-		D13738at2759
OrthoFinder	1	1.000	-	-		FOG0001637
OrthoInspector	1	1.000	-	-		mtm8662
orthoMCL	1	0.900	-	-		OOG6_102664
Panther	1	1.100	-	-	O	PTHR13167
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3039
SonicParanoid	1	1.000	-	-		X1528
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	13	12.940