Protein Alignment Piezo and piezo1

DIOPT Version :9

• Sequence 1: NP_001303314.1
• Gene: Piezo / 34112
• FlyBaseID: FBgn0264953
• Length: 2973
• Species: Drosophila melanogaster
• Sequence 2: XP_696355.4
• Gene: piezo1 / 567949
• ZFINID: ZDB-GENE-041111-255
• Length: 2538
• Species: Danio rerio
• Alignment Length: 3168
• Identity: 815/3168 - (25%)
• Similarity: 1331/3168 - (42%)
• Gaps: 859/3168 - (27%)

  Fly     1 MVFSYACMVLQRIVVPAVLVLAALMRPVGISFVYLLMFFVSPFVPLATRRNFKGSVTAFFIILLT 65
            |.....|.:|...::|..|:.|.:.|...:|.||||...:.|:.....:...:|....:...|.:
Zfish     1 MELQVVCGLLYCCLLPIFLLAACIFRYNALSLVYLLYLLLLPWFQWPNKHTLRGHTGCYIKALFS 65

  Fly    66 LSTLVLLGHITLQILAVSLTLPI------YNCSFSERLLRHIGFVSFIDLQ-PFAIIEWLVPEVL 123
            .|.:.:|||:|.||..  .|:|.      :|||..|.|.||:| ||.:.|: |..::..|.|::.
Zfish    66 TSLIFILGHVTFQICL--YTIPSLDDALGHNCSSWETLSRHVG-VSRLPLEDPQGVLWLLTPDLG 127

  Fly   124 VFATSLGSYLTVKRV------ASQP-------VGAEQLENGEVVDGQAENAQTSSQPSAADANGG 175
            ||..||.:.:...|:      .|.|       ...|..::.|.|:|:.|...:.|:....:
Zfish   128 VFIMSLITLILCSRLLKKRDEGSVPHMSALLHEADETEDDDEDVEGEDEGMLSCSETEDEE---- 188

  Fly   176 DVQQATVTTPLQQQQQQLRKRVSMISQHIHFEGLVKISPLFCLATLFFAAVLRPSVPGGFYFLIF 240
                    :|......||..|:...:|..    |..:..:..:..|..|.:..||....||||:|
Zfish   189 --------SPSSSSAAQLAARLRATAQRF----LRNMGRILAVTLLALAGITLPSAFSAFYFLLF 241

  Fly   241 LLSGTYWATCQTLQR-GFALLLRCVMVVLVL--HSLSIVSYQTPWMQSHLNHTTLTARLIGLEPL 302
            :...|:||....:.. ||..|  ||||....  |.:.:..||:.:.|:..:...|.|||.||:.|
Zfish   242 IGVCTWWACHFPISHLGFNAL--CVMVAFFTGGHLVCLYLYQSSFAQAMFSPAGLWARLFGLKDL 304

  Fly   303 IESYCSPDIRVFLYNNKLSLDSYLNPFALFFAYFALALTTKHLIKPRLVRQSTRKARTPQPLESG 367
            :.........|.| |.:.....|:||..|...|.::.:..|  |....:..:. |||      .|
Zfish   305 VTPGNCTTFEVTL-NTQYDWPVYVNPGILLLLYISVTIVLK--INSNSLGDAD-KAR------EG 359

  Fly   368 SSVAPSVTQRGNDMQLESMEQR--SEQENTTTSIL----DQISYGFVS----------------- 409
            .:|.....:.|.:::|...|.|  |.:::|..::|    ..:|...::
Zfish   360 EAVKTVQGEAGEEVELRLWEARRQSSEDDTKQTLLTTVDSTLSESHITKEPVINGSSQHDIGPSG 424

  Fly   410 ----------VGGFIYQNSYIFTNILMMAWSIVYHSWLTFVLLLSANVLWMIPNQRKAMMRSSPF 464
                      ||..:.|.|||...|.||.|||.|||||||||||.:.|:||:..:::.....|||
Zfish   425 EATVDNPLRLVGRMVLQQSYICALIAMMVWSITYHSWLTFVLLLWSCVIWMLRTRQRFAAYCSPF 489

  Fly   465 IVLYAEALLIAQYIYGMDLNNEELPTSVPTAGINLQQIGFERPIENQMRPCVPLIVKTAFVLMFW 529
            |:||..||...||::.|||.. |||..:  ..::|.|:|.:|    ...||:.|.....|.|.||
Zfish   490 ILLYGLALCCLQYVWAMDLET-ELPQHI--GSMSLHQLGLDR----AQYPCLRLGALLLFTLTFW 547

  Fly   530 VTSRQFFKEKRDRRRDSTLADFIAPLQ-ITVG-SAGSSYLINDGKKTSKFLKKAGDVIKNLLVRL 592
            :..||..|:...|::..|:     ||| :|.| |.|         :....||..|.::.:|..:.
Zfish   548 LLLRQSVKDTFSRKKSLTV-----PLQEVTTGESTG---------RNESILKVFGGLVMSLYAKY 598

  Fly   593 WIWLLVLVIFLCAITGENMTGFRICYMALFLFFLLVFQSSSKAWVKIMYGFWLFLIFYAMSILIL 657
            ||::...:..:.:..|: :..::|.||.|||..:.::|.....|.:::..||..::.|.|.:||.
Zfish   599 WIYVCGGMFIMVSFAGK-LVAYKIVYMLLFLLCMCLYQVYYSLWRRLLKAFWWMVVAYTMVVLIA 662

  Fly   658 IYTYQFDKFDTYWSDYLNVSATLQKDIGLKRYQTKDLFLHLVSPTIIVILTVIQVHYFHKRFIAS 722
            |||:||:.|..||.::...:.....|:||:.::..:||..:|.|...::..::|:|||||.|:
Zfish   663 IYTFQFEDFPGYWGNFTGFTEQQLADMGLETFKLSELFTSIVIPGFFLLACILQLHYFHKPFM-- 725

  Fly   723 LQQQPLAGGSAQQKPTETTALEPAPSKRRGSAGSLRKSQ---GPSAEAAPGATTDFETSVRDLVR 784
                         |.|:...:.|...||......|.:|.   .|..|.......|:.:...:::.
Zfish   726 -------------KITDLENVSPIHRKRGIDNPDLVQSTEEVRPEEEELIVDQDDYPSEADEVIP 777

  Fly   785 ISFRKIKNKSEYIFKNFKDV-------FWRFLELHIMKAVYIAAFVCSVS--EVCVLHIIFVGFC 840
            ..:..:.::...:.:.|.|.       .||.|||||:|.|  |.||..|:  |...::::.|
Zfish   778 SKWGLVMDRLMVLSRKFSDTLTHVQSFIWRVLELHIVKIV--AFFVVWVALLEPSAMNLVLV--- 837

  Fly   841 VLG--ATSRKAVQVVISRLISFIVTVIVLSKMIYQIEYLSHSQHNVVCSD---NRTA-------- 892
            ||.  |......:.:.|.|.:..|.||::.||:||:..::.::::..|:.   |.|:
Zfish   838 VLWSFAMPYGRFRAMASCLSTIWVCVIIVCKMLYQLSVVNPAEYSNNCTAPMVNETSLEPDEVLN 902

  Fly   893 --------NNAEWIGLTKADKVTGGLMS--LLRTYIIYMVIVTMHAVISLRQLQMRVKIGALNAP 947
                    :.|.|.|:.|.|.|.|.:..  |:...:::...|..|.:...||.|.       :.|
Zfish   903 STLYRASVDPASWFGVRKDDTVLGYVKDHLLVLMLLVFEATVYRHQIHHYRQQQR-------SPP 960

  Fly   948 PTKLLFPNIIRADAEKDLVGLVKYLLNFGFYKFGIEISLIALVSTITYRQDIVAVVYALWLVVLL 1012
            |..::||...|...::.|:..:||:||:.|||||:||..:..|:.|..|.:.:.:::..|||.::
Zfish   961 PIPVIFPQATRDTLDQGLLHCIKYMLNYSFYKFGLEICFLMTVNVIGQRMNFLVIIHGCWLVAIM 1025

  Fly  1013 L-LRRSQCAKIWGVFQAFFAISILTQYIVLVGLPPSSCLVFPW---DEGPFGEGIQRWAMLPGAL 1073
            : .||:..|.||..:..|..:.::.||::.||:||:.|:.:||   ........:.:|..||
Zfish  1026 VRRRRANIATIWSKYCLFLVVFMIYQYVLCVGIPPALCIDYPWRWRSTVTIHSALVKWMYLP--- 1087

  Fly  1074 HFNHVP---KLIFDFIVLVILNRQKSIFCIEQRYASNDDY--PGGSNRSVIADIAQLGRVPFD-- 1131
            .|..||   .||.||::|:..::|.::|..|::    :::  .||.||.        ...|.:
Zfish  1088 DFYTVPNSKNLISDFLLLMCASQQWTVFDNEKK----EEWMVMGGENRD--------NPDPMENM 1140

  Fly  1132 --NPTHDFCSYIRNYSDILKNGVLCGFYWFTLAVVFLAGTNIADLLALGYLIGAFIFLWQGSDFY 1194
              ||..:|.: .|:|.|:.|..|....:||.|:|||:.|.....:..||||:..|.||..|:...
Zfish  1141 LFNPAPNFIN-CRSYLDMAKILVFRYMFWFVLSVVFVTGATRISVFGLGYLMACFFFLLFGTKLL 1204

  Fly  1195 LRPIHTIIFRWKWLLAFNVANILIKTSFQMAGCLFMTQLTKDCCWLVHMLGITCT-------SNV 1252
            .:|..|.:..|..|:.:|||.|:.|....:..|:|:.::.|:.||::.:..:.||       .:|
Zfish  1205 TKPSRTRLVMWDCLIIYNVAVIISKNILSILACVFVMEMQKNFCWVIQLFSLVCTVKGYYDPESV 1269

  Fly  1253 LTEQIMLPEEAELALKPGECPKITHQVVLLWDTICFAFIIFQLRIFKSHYFCHIITDTKANNILA 1317
            ..:...||.|               :..::||:|||.|::.|.|:|.|.||.|:..|.:|:...|
Zfish  1270 CNKDCSLPVE---------------EAGIIWDSICFFFLLLQRRVFLSFYFLHVSADLQASARQA 1319

  Fly  1318 SRGADIIESLRHKQIAHRHDH---EKQVLHKIKRKMERIRATQQKMLRPLDKQTHFDGRRTPSEH 1379
            |||   .|..|...|.:.|.|   |::.:.::|..|:|||..|||         :.|||.|
Zfish  1320 SRG---FELFRASIIKNMHFHQQAERKSIQQLKESMKRIRGKQQK---------YKDGRIT---- 1368

  Fly  1380 AMSDVAPLVHNSSFTSCQGSGYLTPDEHSSATSGTSSPARASILSSSGGSVESVDSADAAVIIEP 1444
              ||:
Zfish  1369 --SDI------------------------------------------------------------ 1371

  Fly  1445 EINVMPCDEITDYVDVQSVSEEETTVAMPKKDLSCQSKECVFKSPEPSKPTTPSTDALSTLLTEG 1509
                                 ||                      :||.|
Zfish  1372 ---------------------EE----------------------QPSDP--------------- 1378

  Fly  1510 FLEPKRISLGLAPPELSPPVGMQCLAPPLAAYATAMASSAASSVLSTNLTPNLRREHRRQSSTNA 1574
                                                                  :|.::::..:.
Zfish  1379 ------------------------------------------------------KEQKKETKLSK 1389

  Fly  1575 AVSWNETVSIKRSPMKKQMSADKEEVVTMRHKSLHRRHQSMGNASYSLDEASQSQRKRLSSNLSG 1639
            :.||.
Zfish  1390 SKSWQ------------------------------------------------------------ 1394

  Fly  1640 ARDEAALRSAQRPHSWGPVGTSSYMESLMCAFYEPALLHGPSTRAGDYYMFEEMDDKFELDLIHD 1704
                         :.|....|               :||     :|:|||||.
Zfish  1395 -------------YPWVDHAT---------------VLH-----SGEYYMFES------------ 1414

  Fly  1705 EIDFLEEENITESEMKMQRR---KTLYDVW-KDLNDAEYRRHFYMRERSYASEPGSRILLKLDDE 1765
              |..||:...:.|.|.:|:   :..|..| ....||...|.  .|:|...:|.          |
Zfish  1415 --DSGEEDETFQEEQKPRRQTAFQLAYQAWVTSAKDALKERQ--QRQRKQRAEA----------E 1465

  Fly  1766 KEKIGPDLGLASDHDEDDDTIECDLGMRTSTLSEPLDFGRRSQTLLEVKSKDAPTGEFPSTSKGI 1830
            |::...    |:|..|:.:|:              .| |..||...|::.:
Zfish  1466 KQQNAQ----ATDDAEEGETV--------------FD-GENSQHAEEMQEE-------------- 1497

  Fly  1831 SKERDAATASSSASPAPTRDVGDLPVIPPPSTGLGREQTSKETSDSKSKMEVDSGEVTAKDSDED 1895
                                              |.:..|:.|.....:..:|
Zfish  1498 ----------------------------------GEDAASQATGSDMVQRILD------------ 1516

  Fly  1896 FDTNPIIRLL--------EGFLVTLTIRLNRFSRNYRFVNRILAGEKKTLKESSSLNRLGLSSAA 1952
                 |::.|        :||    |:.||..::.|...:.:|:.|:.....:       :|..|
Zfish  1517 -----ILKFLWVLFLAMVDGF----TMWLNLLTKQYVDTSMVLSEERYLFIHN-------VSQRA 1565

  Fly  1953 AMFHFLKSNLESDESEPPASSSTPRRVVIAPPNATEHSDPTSTTLNTNTTTTPLSPPEPLQPLQP 2017
            :     :.|::...|.                   :..|.|..|....|.|
Zfish  1566 S-----RENMDDQISH-------------------DSEDLTLETCLDETDT-------------- 1592

  Fly  2018 LQPNTTSTPQQQHQHIRAAEEIIELPVDTVDGVAHRKQSINSSPPAKGTMLSRKSDCGLPEIRIK 2082
              .|...|            .::|  .:|:.|.|   :|.:.|.....:.|:.:.||
Zfish  1593 --DNICDT------------NVVE--SETLGGFA---KSRDFSSTTLSSELTLELDC-------- 1630

  Fly  2083 APSIERGAHYYHNHHSGGGSGSLSKH-WSYEQVDSAGEFNLEEENFAQRDHHIIVEVLISSWYAL 2146
            .|...:..|  ..|.|...|..||:. :..:::..:.||...:..        ::::|.:.:..|
Zfish  1631 EPFYSQQTH--RPHRSRTASELLSERPFRVDELIQSREFYSSQNR--------LLKLLFALYNVL 1685

  Fly  2147 LANTDLICYIVVFINQVVNASLISLPLPIMVFLWGTLSLPRPTKTFWVTLIAYTQAIVLIKCIFQ 2211
            .|:::|:||.::.:|.:|.||:|||.||::||||..||:|||:|.||:|.|.||:.:|::|.:||
Zfish  1686 AAHSELVCYFIIVLNNLVTASVISLVLPVLVFLWAMLSVPRPSKRFWMTAIIYTEVMVVVKYLFQ 1750

  Fly  2212 FKLI-WSNYHQL---PNQPLTPAKIFGVENKAHYAIYDLILLLVLFLHRYLLKSQGLW------- 2265
            |... |:..::|   .::|..|.:|.|:|...:|..|||:.|||||.||.||:..|||
Zfish  1751 FGFFPWNTEYELKLNEDKPFFPPRILGLEKTDNYIRYDLLQLLVLFFHRSLLQRYGLWDQEGPLE 1815

  Fly  2266 -KSGYKDTDNQFTKPTASIDER-----DDSDNLSQPD---------------------------- 2296
             ||.....:|...:.    ||:     |:.|:.:||:
Zfish  1816 EKSDLSPAENSKNEN----DEKKFQHSDEEDSQAQPEPNTEVLSLTPSAETPTDAEIQTDPEPNS 1876

  Fly  2297 --------------------------SRQLNDDAAQK-LSLQVSQASLPGSPEFSKTGINQLERT 2334
                                      .::...||.|| ...:..:.....|.|.||..:|.:| .
Zfish  1877 HTNNTKTSDTVKNDGMKKSHFLRFRKKKKAEPDAVQKEPKKKKKKCKTKHSKETSKKLLNAIE-G 1940

  Fly  2335 KYTS-------SLYK----FFFSLVH-KSRLATDVYALMFLCDFVNFFVLLFGFTAFGTQQTESD 2387
            |:.|       ::|:    ||.:::| :.|.:|||||||||.|.|:|.:::|||.|||.....:|
Zfish  1941 KFKSLFLSVVKNVYRPTWDFFQNILHAEYRASTDVYALMFLTDVVDFIIIIFGFWAFGKHSAAAD 2005

  Fly  2388 EGVQTYLAENKVPIPFLIMLLVQFLLIVIDRALYLRKALVNKIIFHFFSVIGIHIWMFFVVPAVT 2452
              :.:.|:|::||..||:|||:||..::|||||||||:::.|:||....|.|||:||||::||||
Zfish  2006 --IASSLSEDQVPEAFLVMLLIQFSTMIIDRALYLRKSILGKLIFQVILVFGIHLWMFFILPAVT 2068

  Fly  2453 ERTFNSLAPPIIFYVIKCFYMLLSSYQIKSGYPKRILGNFFTKGFSMVNMIAFKVYMQIPFLYEL 2517
            ||.||..:...::|..||.|..||:|||:.|||.||||||.||.|:.:|:..|:.:..:|||.||
Zfish  2069 ERMFNHNSVAQLWYFFKCIYFTLSAYQIRCGYPTRILGNFLTKKFNHLNLFLFQGFRLVPFLVEL 2133

  Fly  2518 RTILDWVCIDSTMTIFDWLKMEDIFSNIYLIRCTRQSETDFPAMRAQKKASLSKLIMGGTIVLLI 2582
            |.::|||..|:|:::.:|:.:|||::||::|:|:|::|..:|..:.|||..:.|..|||.|:|.:
Zfish  2134 RAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKRIVKYGMGGLIILFL 2198

  Fly  2583 VICIWGPLCLFAL-GNAVGTSNVPFHVSLSIRIGPYDPIYTTN-NYDSIFEINPEMYSQMTNAYI 2645
            :..||.||...:| .:.||..|.|..|::::::|.|:|::|.: ...||.......|:|:.|.:.
Zfish  2199 ICIIWFPLLFISLVKSVVGVVNHPVDVTVTVKLGGYEPLFTMSVQQQSIQPFTESRYNQLNNQFS 2263

  Fly  2646 KEKQALTFIAGYDATDVAAVRLAGNSPSLWNIAPPDRQRLLND-LRNNHTLKARFSYSLTRKAPA 2709
            |...|:.||..|...|:....:.|:|.|:|.|:||.||.|:.: |.:...:..|..::..|....
Zfish  2264 KNAVAMQFITMYSYEDIVTANIEGSSGSVWRISPPSRQELIKELLSSTGDMTLRLDWNFQRDLGK 2328

  Fly  2710 KGLKENVGDEHAISLDESFEGRAALIHMLSETHDVEPIYSNGTTNGTTPEVEEVVVIPGMIPKFI 2774
            .|..||..|:|:|||......||.|..:|..|.. :|::                 :|.|.|.:|
Zfish  2329 GGTVENTFDKHSISLAPKNPVRADLASLLLGTRK-DPVH-----------------VPHMFPNYI 2375

  Fly  2775 KVLNSGDA-AVVSVLSPKHYDYRPLVIKMHRDNETNGL----WWEI-----RDYCNDTFYNETLS 2829
            :..:..:| .|:.:.......::.:.:.:.|:...|..    ||:|     ::.||
Zfish  2376 RAPSGAEAKPVMQLFDGDEEGFQDVTVSLMREASVNTTGAQEWWDISIADCKESCN--------- 2431

  Fly  2830 KFAYSNCTSGIVMYTFNDKKFPSTFSFLTAGGIIGLYTTFVLLASRFMKSFIGGQNRKIMFEDLP 2894
                     .:.|..||||..|.:..||...||:|||.:.||:..:|::.|....:..||||:||
Zfish  2432 ---------VLPMIIFNDKVSPPSLGFLAGYGIMGLYVSVVLVIGKFVRGFFSEISHSIMFEELP 2487

  Fly  2895 YVDRVLQLCLDIYLVREALEFALEEDLFAKLLFLYRSPETLIKWTRPK 2942
            ..||:|:||:||:||||..|..|||:|::||:||||||||:|||||.|
Zfish  2488 CADRILKLCMDIFLVRETGELELEEELYSKLIFLYRSPETMIKWTREK 2535

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Piezo	NP_001303314.1	PIEZO	1226..>1363	CDD:292539	44/146 (30%)
		Piezo_RRas_bdg	2511..2941	CDD:288978	149/442 (34%)
piezo1	XP_696355.4	PIEZO	1235..1452	CDD:292539	78/528 (15%)
		Piezo_RRas_bdg	2126..2534	CDD:288978	149/443 (34%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170583720
Domainoid	1	1.000	427	1.000	Domainoid score	I585
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1038	1.000	Inparanoid score	I263
OMA	1	1.010	-	-		QHG46055
OrthoDB	1	1.010	-	-		D13738at2759
OrthoFinder	1	1.000	-	-		FOG0001637
OrthoInspector	1	1.000	-	-		mtm6604
orthoMCL	1	0.900	-	-		OOG6_102664
Panther	1	1.100	-	-	LDO	PTHR13167
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3039
SonicParanoid	1	1.000	-	-		X1528
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	14	13.940