DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Piezo and piezo1

DIOPT Version :9

Sequence 1:	NP_001303314.1	Gene:	Piezo / 34112	FlyBaseID:	FBgn0264953	Length:	2973	Species:	Drosophila melanogaster
Sequence 2:	XP_002933721.3	Gene:	piezo1 / 100488940	XenbaseID:	XB-GENE-6050252	Length:	2474	Species:	Xenopus tropicalis

Alignment Length:	3089	Identity:	809/3089 - (26%)
Similarity:	1305/3089 - (42%)	Gaps:	761/3089 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MVFSYACMVLQRIVVPAVLVLAALMRPVGISFVYLLMFFVSPFVPLATRRNFKGSVTAFFIILLT 65
            |.....|.||..:::|..|:.|.|.|...:|.|||....:.|:.......:.:|........||.
Frog     1 MELQVLCGVLFWLLLPGSLLAACLFRYNSLSLVYLFYLLLLPWFAGPNPCSMRGHTGRLMKALLA 65

  Fly    66 LSTLVLLGHITLQILAVSLTLPI------YNCSFSERLLRHIGFVSFIDLQPFA-IIEWLVPEVL 123
            .|.|.|..|:..||..  .|:|.      :|||..:.|.||:| ::.:||.... ::..:.|:..
Frog    66 TSILFLCAHLIFQICL--YTIPELDDLLGHNCSSWDVLARHVG-INRLDLSDIPNMVRQVSPDFG 127

  Fly   124 VFATS-----LGSYLTVKRVASQPVG-AEQLENGEVVDGQAENAQTSSQPSAADANGGDVQQATV 182
            :|..|     |.:.|..|:...:..| .||.|....::.:.|:.:..::...    |||:.....
Frog   128 MFVVSALCLGLCNRLVTKKRRQRSSGYFEQEEERTELNLKYESRECDTEFEV----GGDLHSVPR 188

  Fly   183 TTPLQQQQQQLRKRVSMISQHIHFEGLVKISPLFCLATLFFAAVLRPSVPGGFYFLIFLLSGTYW 247
            ...|   ...|||..        :..|..:.....:..|..|.:..||.....|:::|:...|:|
Frog   189 DRLL---GSWLRKAA--------YRLLETVEKSLAVLLLALAGITDPSAFSAPYYVVFIGLCTWW 242

  Fly   248 ATCQ--TLQRGFALLLRCVMVVLVL--HSLSIVSYQTPWMQSHLNHTTLTARLIGLEPLIESYCS 308
            | |.  ..|.||..:  ||:|....  |.:.:..||||::|...:...|.|||.||..:|.....
Frog   243 A-CHFPISQLGFNTI--CVIVGFFTGGHMICLYIYQTPFIQQMFHPEQLWARLFGLHNIISHSNI 304

  Fly   309 PDIRVFLYNNKLSLDSYLNPFALFFAYFAL--ALTTKHLIKPRLVRQSTRKAR------------ 359
            .|......|..|....|::|..|...|::|  .|..|...|.|:.....||::
Frog   305 TDCTHDSLNQNLDWPVYVHPGILLLLYYSLLSILKLKSSDKKRISGCDLRKSQQEDMELTDIEPV 369

  Fly   360 TPQPLESGSSVAPSVTQRGNDMQLESMEQRSEQENTTTSILDQIS---------------YGFVS 409
            :.:...:|....| :.||.|      .|:.::.::.|..::.:.|               :..:.
Frog   370 SKEQYSAGEETKP-MLQRMN------TEEEAKPQDCTVHVIRRNSNQLPEELETKEPSQFHLMIH 427

  Fly   410 VGGFIYQNSYIFTNILMMAWSIVYHSWLTFVLLLSANVLWMIPNQRKAMMRSSPFIVLYAEALLI 474
            |   |...|||...|.||.|||.|||||||||||.|.::||:.|:|:..|..||||::|...|..
Frog   428 V---ILSQSYICALIAMMVWSITYHSWLTFVLLLWACLIWMVRNRRQFAMLCSPFILMYGVTLCS 489

  Fly   475 AQYIYGMDLNNEELPTSVPTAGINLQQIGFER-PIENQMRPCVPLIVKTAFVLMFWVTSRQFFKE 538
            .||::.||| .|||||.:  ..:.|.|:|..| |.     ||:.:.....:.|.||:...||..|
Frog   490 LQYVWSMDL-REELPTQL--GFMKLSQLGLVRAPY-----PCLNMGALLLYTLTFWLLLHQFVTE 546

  Fly   539 KRDRRRDSTLADFIAP-LQITVGSAGSSYLINDGKKTSKFLKKAGDVIKNLLVRLWIWLLVLVIF 602
            |..:|:     .|::| |::||..||         |....||:.|:.:|:...:.||::...:..
Frog   547 KFLKRK-----KFMSPLLEVTVADAG---------KEKNLLKRFGEQVKSFYAKYWIYVCGGMFI 597

  Fly   603 LCAITGENMTGFRICYMALFLFFLLVFQSSSKAWVKIMYGFWLFLIFYAMSILILIYTYQFDKFD 667
            :.:..| .:..::|.||.|||..|::||.....|.|::..||..::.|.|.:||.|||:||::|.
Frog   598 VVSFAG-RLVVYKIVYMLLFLLCLVLFQVYYSLWRKLLTFFWWLVVAYTMLVLITIYTFQFEEFP 661

  Fly   668 TYWSDYLNVSATLQKDIGLKRYQTKDLFLHLVSPTIIVILTVIQVHYFHKRFIASLQQQPLAGGS 732
            .||.:..|::.....||||:|:...:||..::.|...::..::|:||||:.|   :....||..|
Frog   662 EYWKNLTNLTDEQLGDIGLERFSVSELFTSILIPGFFLLACILQLHYFHRPF---MHMTNLAHAS 723

  Fly   733 AQQKPTETTALEPAPSKRRGSAGS--LRKSQGPSAEAAPGATTDFETSVRDLVRISFRKIKNKSE 795
            .|.......:.:|...:.:..|.|  ....:....|:|.||    :.|..|||......:..|..
Frog   724 CQGSFHSHESEQPKEEEEKAGACSDAAADEEEDEEESAHGA----DLSKWDLVTQRLSVLSFKFS 784

  Fly   796 YIFKNFKDVFWRFLELHIMKAVYIAAFVCSVSEVCVLHIIFVGFCVLGATSR--KAVQVVISRLI 858
            .:....:...||.||||::|.|.:.:...::.||.|::.:||   ||.|.:.  ...|.:.|.|.
Frog   785 ELLHRLQVFLWRLLELHVLKLVALYSVWVALQEVSVMNFLFV---VLWAFAMPFSRFQHMASCLC 846

  Fly   859 SFIVTVIVLSKMIYQIEYLSHSQHNVVCSD---NRTA----------------NNAEWIGLTKAD 904
            :...::|::.||:||:..::..:::..|:.   |.|:                :.|.|.|:.|..
Frog   847 TIWTSIIIVCKMLYQLTVVNPLEYSSNCTQPPLNNTSLLEDEILNSTLYREPIDPANWFGIRKGY 911

  Fly   905 KVTGGLMSLLR--TYIIYMVIVTMHAVISLRQLQMRVKIGALNAPPTKLLFPNIIRADAEKDLVG 967
            ...|.:.:.|:  ..:::..||..|.....:|.|       |..|..:.:||::.|...::.|..
Frog   912 PNLGYIQNHLQILLLLVFEAIVYRHQEFYRKQYQ-------LEKPHIQAVFPDVYREHLDQGLFS 969

  Fly   968 LVKYLLNFGFYKFGIEISLIALVSTITYRQDIVAVVYALWLVVLLLLR-RSQCAKIWGVFQAFFA 1031
            .:||.:|:.|||||:||..:..|:.|..|.:.:.:.:..||:|:|..| |...||:|..:..|..
Frog   970 CIKYFINYSFYKFGLEICFLLTVNVIGQRMNFMVIFHGCWLIVILTRRQRVNIAKLWPKYCVFLI 1034

  Fly  1032 ISILTQYIVLVGLPPSSCLVFPWDEG---PFGEGIQRWAMLPGALHFNHVPK---LIFDFIVLVI 1090
            ..:|.||::.:|:||..|:.:||...   |....:.:|..||   .|...||   ||.||::|:.
Frog  1035 FFLLWQYMICLGIPPVLCIDYPWRWDTFVPVNSALIKWLYLP---DFYTTPKSVNLISDFVLLIG 1096

  Fly  1091 LNRQKSIFCIEQR-------------YASNDDYPGGSNRSVIADIAQLGRVPFDNPTHDFCSYIR 1142
            :.:|..:|..|::             |.:..|.|                    ||..:|.: .:
Frog  1097 VAQQWKVFINEKKEEWYTMAGENVEEYEAPKDRP--------------------NPVPNFIN-CQ 1140

  Fly  1143 NYSDILKNGVLCGFYWFTLAVVFLAGTNIADLLALGYLIGAFIFLWQGSDFYLRPIHTIIFRWKW 1207
            :|.|::|..|....:||.|.|||:.|.....:..||||:..|..|..|:....:|:...:..|..
Frog  1141 SYLDMVKVAVFRYLFWFVLTVVFITGATRISVFGLGYLMSCFYLLLSGTTLLQKPMKAKLVLWDC 1205

  Fly  1208 LLAFNVANILIKTSFQMAGCLFMTQLTKDCCWLVHMLGITCTSNVLTEQIMLPEEAELALKPGEC 1272
            |:.:|:..||.|....:..|:|:.|:..:.||::.:..:.||    .:....|::.:   |..:|
Frog  1206 LILYNITVILSKNMLSLLSCVFVQQMQTNFCWVIQLFSLVCT----VKGYYNPQDMD---KDRDC 1263

  Fly  1273 PKITHQVVLLWDTICFAFIIFQLRIFKSHYFCHIITDTKANNILASRGADIIESLRHKQIAHRHD 1337
            .....:..::||:|||.|::.|.|:|.|.||.|::.|.:|:::..|||.::..:...|.:....:
Frog  1264 SLPVEEAGIIWDSICFLFLLLQRRVFLSCYFRHVVLDLRASSLQTSRGFELFMASIRKNVFFHQE 1328

  Fly  1338 HEKQVLHKIKRKMERIRATQQKMLRPLDKQTHFDGRRTPSEHAMSDVAPLVHNSSFTSCQGSGYL 1402
            .|::.|::::::||||||.|::         :.:|||......:.:..|       .|.:||
Frog  1329 SERRSLNQLRKQMERIRAKQER---------YHEGRRQTDSSQVKEGEP-------GSKKGS--- 1374

  Fly  1403 TPDEHSSATSGTSSP--ARASILSSSGGSVESVDSADAAVIIEPEINVMPCDEITDYVDVQSVSE 1465
                 |......|.|  ..||:|.|.                             ||...:|.||
Frog  1375 -----SRQKIHWSRPWLDHASVLHSG-----------------------------DYFLFESDSE 1405

  Fly  1466 EETTVAMPKKDLSCQSKECVFKSPEPSKPTTPSTDALSTLLTEGFLEPKRISLGLAPPELSPPVG 1530
            |:                                       .|..||
Frog  1406 ED---------------------------------------EESLLE------------------ 1413

  Fly  1531 MQCLAPPLAAYATAMASSAASSVLSTNLTPNLRREHRRQSSTNAAV-SWNETVSIKRSPMKKQMS 1594
             |...||                              :||:...|. :|   |:..:|.:|::.|
Frog  1414 -QDQRPP------------------------------KQSAFQLAYQAW---VTSAKSALKERKS 1444

  Fly  1595 ADKE-----EVVTMRHKSLHRRHQSMGNASYSLDEASQSQRKRLSSNLSGARDEAALRSAQRPHS 1654
            ..||     |:||                              :.:|.:|.
Frog  1445 RQKERSGKVEMVT------------------------------VENNEAGG-------------- 1465

  Fly  1655 WGPVGTSSYMESLMCAFYEPALLHGPSTRAGDYYMFEEMDDKFELDLIHDEIDFLEEENITESEM 1719
                ||.|:.:.             |..:..|..:.||......:..|.|.:.||
Frog  1466 ----GTESHTQE-------------PREKGEDKDLNEERGGSNVVQRILDVLKFL---------- 1503

  Fly  1720 KMQRRKTLYDVWKDLNDAEYRRHFYMRERSYASEPGSRILLKLDDEKEKIGPDLGLASDHDEDDD 1784
                       |                        ..:|..:|.....:|.   |...|.:...
Frog  1504 -----------W------------------------VLLLAMVDGLINWLGT---LTKQHVDMST 1530

  Fly  1785 TIECDLGMRTSTLSEPLDFGRRSQTLLEVKSKDAPTGEFPSTSKGISKERDAATASSSASPAPTR 1849
            .:..:..:.|..||:..|..||.   ||   |...||:
Frog  1531 VLWVERYIITQKLSKGEDINRRD---LE---KVYETGQ--------------------------- 1562

  Fly  1850 DVGDLPVIPPPSTGLGREQTSKETSDSKSKMEVDSGEVTAKDSDEDFDTNPIIRLLEGFLVTLTI 1914
            ||.:..:..||        |...||.:.|..|
Frog  1563 DVREPRISSPP--------TDMSTSIASSGCE--------------------------------- 1586

  Fly  1915 RLNRFSRNYRFVNRILAGEKKTLKESSSLNRLGLSSAAAMFHFLKSNLESDESEPPASSSTPRRV 1979
                                                               |.|          :
Frog  1587 ---------------------------------------------------EEE----------L 1590

  Fly  1980 VIAPPNATEHSDPTSTTLNTNTTTTPLSPPEPLQPLQPLQPNTTSTPQQQHQHIRAAEEIIELPV 2044
            :.:....||:....||..||.:.:                                        :
Frog  1591 ICSTAECTENITQLSTGYNTRSNS----------------------------------------L 1615

  Fly  2045 DTVDGVAHRKQSINSSPPAKGTMLSRKSDCGLPEIRIKAPSIERGAHYYHNHHSGGGSGSLSKHW 2109
            |::.|....|.| :.|..|...:|||..|  .||:.
Frog  1616 DSIRGFPEIKPS-HRSRTASELLLSRPLD--FPELS----------------------------- 1648

  Fly  2110 SYEQVDSAGEFNLEEENFAQRDHHIIVEVLISSWYALLANTDLICYIVVFINQVVNASLISLPLP 2174
                         |.::|.|....|: .:|::.:..:.|:::|:||.::.:|.:|:||||||.||
Frog  1649 -------------ESQHFFQSQSRIL-RLLLALYNFVAAHSELLCYFIIILNNMVSASLISLFLP 1699

  Fly  2175 IMVFLWGTLSLPRPTKTFWVTLIAYTQAIVLIKCIFQFKLI-WSNYHQL---PNQPLTPAKIFGV 2235
            |::||||.||:.:|:|.||:|.|.:|:.:|:||.:|||... |:||..|   ..:|..|.::.|:
Frog  1700 ILIFLWGMLSIHKPSKRFWMTAIIFTEVMVVIKYLFQFGFFPWNNYTILVRNEGKPFFPPRVLGL 1764

  Fly  2236 ENKAHYAIYDLILLLVLFLHRYLLKSQGLWKSGYKDTDNQFTKPTASIDERDDSDNLSQPDSRQL 2300
            |....|..||||.||:||.||.||.|.|||..  ||.|.:...|     .:|.........|:.|
Frog  1765 EKSDSYIKYDLIQLLMLFFHRSLLMSYGLWDR--KDLDLEERTP-----GKDKGSKTEIQASQDL 1822

  Fly  2301 NDDAAQKLSL-----QVSQASLPGSPEFSKTGINQLERTK--------------------YTSSL 2340
            ........||     :.||.|:.......:..::..::.|                    ...::
Frog  1823 EPAGGSSSSLRQRKRKESQGSVQERDSLKEKSLDVQKKKKGRVALRVKIMGMKMQQFFACIVRNI 1887

  Fly  2341 YK----FFFSLVH-KSRLATDVYALMFLCDFVNFFVLLFGFTAFGTQQTESDEGVQTYLAENKVP 2400
            |:    ||..::| |:|.||||||.|||.|.|:|.:::|||.|||.....:|  :.:.|::::||
Frog  1888 YQPVKAFFHDILHTKNRAATDVYAFMFLADVVDFVIIVFGFWAFGKHSAAAD--IASSLSDDQVP 1950

  Fly  2401 IPFLIMLLVQFLLIVIDRALYLRKALVNKIIFHFFSVIGIHIWMFFVVPAVTERTFNSLAPPIIF 2465
            ..||:|:|:||..:||||||||||.::.|:||....|||||:||||::||||||.||......::
Frog  1951 EAFLVMVLIQFSTMVIDRALYLRKTVLGKLIFQVILVIGIHVWMFFILPAVTERMFNQNTVAQLW 2015

  Fly  2466 YVIKCFYMLLSSYQIKSGYPKRILGNFFTKGFSMVNMIAFKVYMQIPFLYELRTILDWVCIDSTM 2530
            |.:||.|..||:|||:.|||.||||||.||.::.:|:..|:.:..:|||.|||.::|||..|:|:
Frog  2016 YFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTL 2080

  Fly  2531 TIFDWLKMEDIFSNIYLIRCTRQSETDFPAMRAQKKASLSKLIMGGTIVLLIVICIWGPLCLFAL 2595
            ::.:|:.:|||::||::|:|:|::|..:|..:.|||..:.|..|||.|::.::..||.||...:|
Frog  2081 SLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIIVFLICIIWFPLLFMSL 2145

  Fly  2596 -GNAVGTSNVPFHVSLSIRIGPYDPIYTTN-NYDSIFEINPEMYSQMTNAYIKEKQALTFIAGYD 2658
             .:.||..|.|..|:::.::|.|:|::|.: ...||....|:.|..:|..:.::..|:.||..|.
Frog  2146 VRSVVGVVNHPIDVTVTFKLGGYEPLFTMSAQQQSIQPFTPQQYEALTYEFERQPTAMQFITLYS 2210

  Fly  2659 ATDVAAVRLAGNSPSLWNIAPPDRQRLLNDLRNNHT-LKARFSYSLTRKAPAKGLKENVGDEHAI 2722
            ..|:...|:.|:|.|:|.|:||.|:::..:|:|..: :..||::...|.....|..|:..::|..
Frog  2211 YEDIVTARIEGSSGSVWGISPPSREQMRKELQNGSSDITLRFTWDFQRDLAKGGTVEHTSEKHTK 2275

  Fly  2723 SLDESFEGRAALIHMLSETHDVEPIYSNGTTNGTTPEVEEVVVIPGMIPKFIKVLNSGDAAVVSV 2787
            .|:...:.|..|..:|....|             ||     |.|..:.||:|:..|..:|..|..
Frog  2276 DLEPGSQERMELADLLQGVRD-------------TP-----VSIQHLFPKYIRAPNGPEANPVKQ 2322

  Fly  2788 LSP-KHYDYRPLVIKMHR-----DNETNGL--WWEIRDYCNDTFYNETLSKFAYSNCTSGIVMYT 2844
            |.| :...|..:.::::|     .|.|:..  ||.|           ||......:|.. :.|..
Frog  2323 LQPDEEESYLDVEVQLNRMRVAEGNSTSSFVEWWVI-----------TLKDCEGEDCNI-LPMVI 2375

  Fly  2845 FNDKKFPSTFSFLTAGGIIGLYTTFVLLASRFMKSFIGGQNRKIMFEDLPYVDRVLQLCLDIYLV 2909
            ||||..|.:..||...||:|||.:.||:..:|::.|....:..||||:||.|||:|:||.|||:|
Frog  2376 FNDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIYVV 2440

  Fly  2910 REALEFALEEDLFAKLLFLYRSPETLIKWTRPKE 2943
            ||..|..|||:|:|||:||||||||:||||..|:
Frog  2441 RERGELELEEELYAKLIFLYRSPETMIKWTVDKD 2474

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Piezo	NP_001303314.1	PIEZO	1226..>1363	CDD:292539	38/136 (28%)
Piezo	NP_001303314.1	Piezo_RRas_bdg	2511..2941	CDD:288978	153/440 (35%)
piezo1	XP_002933721.3	PIEZO	1223..1445	CDD:406366	71/372 (19%)
piezo1	XP_002933721.3	Piezo_RRas_bdg	2060..2470	CDD:403405	151/439 (34%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	440	1.000	Domainoid score	I541
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG46055
OrthoDB	1	1.010	-	-		D13738at2759
OrthoFinder	1	1.000	-	-		FOG0001637
OrthoInspector	1	1.000	-	-		mtm9559
Panther	1	1.100	-	-	LDO	PTHR13167
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3039
SonicParanoid	1	1.000	-	-		X1528
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	11	11.060