Protein Alignment Megf8 and megf8

DIOPT Version :10

• Sequence 1: NP_609180.3
• Gene: Megf8 / 34099
• FlyBaseID: FBgn0031981
• Length: 2892
• Species: Drosophila melanogaster
• Sequence 2: XP_005158088.1
• Gene: megf8 / 791154
• ZFINID: ZDB-GENE-090730-1
• Length: 2870
• Species: Danio rerio
• Alignment Length: 3088
• Identity: 1033/3088 - (33%)
• Similarity: 1465/3088 - (47%)
• Gaps: 531/3088 - (17%)

  Fly    30 CDRSRKVFTEPYGEISDGPSGFNYTQDSHCEWLIKARNDSQYITLTFHSMGTECSYDYIYVYDGD 94
            |...|:|.....|.::|||.  ||:.:.:|||||.|.:.:..|.|.|..:.|||:|||::|||||
Zfish    25 CKGQRQVLRAVPGYVTDGPG--NYSVNGNCEWLIMAPSRNHRIVLNFMFIDTECTYDYLFVYDGD 87

  Fly    95 SFNSTLLGSFSGRTQPQRLVARSGSMLILMYSDTNYVLDGFRASYYISNCLNNCHNHGKC--VGH 157
            |:.|.||.|.||:|.||.:.|:||.||:.::||.||.|.||.|:|..|.|..:|..||:|  ...
Zfish    88 SYQSPLLASLSGKTLPQPIEAKSGKMLLHLFSDANYNLLGFNATYTFSVCPGDCSGHGRCDVATA 152

  Fly   158 QCVCHGEWVGPDCEDEACPQRCGESQGRGRCQKS--ICHCSRGYSGRLCDLS--DHPAGSSWRWL 218
            :|.|...|.|.||....|.|.|.: :|.|.|.|.  .|.||.|:.|:.|.|.  |:.....| |.
Zfish   153 RCQCQQGWGGLDCNTPLCSQACTQ-RGHGICDKRGLKCICSSGFVGQDCQLGLRDNSGAGQW-WE 215

  Fly   219 ATDAEGMTA-RAAHSAVYMEDEDALYVFGGYDLNNVISTLQIYRFSTSQWEDEWGIALQSRRHFY 282
            .:..:...| |.:.:.||:....|:|:|||:|||..:..|.:|.|||:.||
Zfish   216 VSSGDVKAAPRTSSAGVYLASTGAMYMFGGFDLNRALEDLVVYNFSTNSWE-------------- 266

  Fly   283 HPQKIDHTLLKAVLQHKNEDEAKLWGLNSDVSFFRNILYTLAESNLHQRRTRSSLPLTIVNANST 347
                                         .::|
Zfish   267 -----------------------------QMTF-------------------------------- 270

  Fly   348 DEELNEYLEDILEEVTDHKPHGRYGHAADSVPGGFVIYGGKHANGSFYSDLWQYNNTESGGKWKQ 412
                            .|.|..|:.|.|....|..|||||:.||.|..||:|.|...::  :|:|
Zfish   271 ----------------SHSPAPRHSHTAVEWQGSMVIYGGELANHSLASDVWIYRPQQN--EWQQ 317

  Fly   413 MAIRSAVKPPALARHTLNTAGSYLYIFGGSLETGEFSSSVYRIPLPLSEDSQWELVQPRGGKTLD 477
            :.:..|...|.||.|.......|||:|||..|...||||:||..|   .:..||.|||.|||  .
Zfish   318 LGLSDAPGAPRLANHAAAIVDHYLYVFGGRTEEDMFSSSLYRFRL---RNGVWEAVQPTGGK--P 377

  Fly   478 VRLAAHSTVYYKATNSLIVFGGIMTSLARFSKLSDRIYAFQLDQMHWTEI--LYPRTALRDTNIP 540
            ...|.||.:|:..:.:|:|:||...:.||||...:...||.:::..||..  .:|      .|.|
Zfish   378 PATAGHSMIYHSPSKTLLVYGGHRPTTARFSVRVNSTDAFHVEKRFWTSFRSRFP------ANGP 436

  Fly   541 RERAFHTATISGNYMVVFGGYTHRHNKDEICYDNQMYWYHLSCHIWINQVVSADDSLYPK---PQ 602
            ||||||:||:.||||||:||..|.|..:|.|||.::::|||.||.|    ||..|||..:   .:
Zfish   437 RERAFHSATVIGNYMVVYGGNVHIHYTEEKCYDEEIFFYHLGCHQW----VSVGDSLGQRGVSVR 497

  Fly   603 GVFAHAAALRRNHTLLIVGGYHGNVNADLFAYELPQVLRVENTLYNPEISCRLHSSHTACLSNPE 667
            |.::|.||:.....||:.|||.|....||.||::|  |.|.:...:.:..|....|.:.||.|||
Zfish   498 GRYSHVAAVMEGRVLLVAGGYSGIARGDLVAYKVP--LFVSSDPGDRDSVCAEAPSESLCLKNPE 560

  Fly   668 CGWCSADSSCYGRTIGANCTTNLQTTRCPGICPSLGDCHSCLVHGSQWGKSSGNKAAFSVASKLG 732
            |.||.      ||......|....:|.|.|:...|.||.||||.       ||...:....|
Zfish   561 CSWCE------GRCREYQPTDPCGSTGCLGLARFLSDCQSCLVF-------SGVPNSLPRVS--- 609

  Fly   733 LNECTWCVQNAKC----HHRDDNYGICGDSSGWWGDKGTEIRRPSLCTSTDRRPGLTYIKYHFPI 793
             .|..|||||..|    .|..........:.||||::...:.....|.:.:..|||..:.:..|.
Zfish   610 -GEFGWCVQNESCLPVSEHSACRVDQISGAYGWWGERTRFLTSLPFCRTENYVPGLHLLTFQHPR 673

  Fly   794 NYTMPDYVGIVNATMVDFASPPFTTYFEHKLEGEMLARLVGFVRPQHQWNNSAIQVCTSYSSAV- 857
            |.:.||.|.|:.:|.: ..:|        ..|.::..:..||:.|  .|::...|...:..:.:
Zfish   674 NQSQPDKVSILRSTSI-ILNP--------STEMDVTLQFRGFIHP--LWSSPPHQETVAMWARIQ 727

  Fly   858 -----LRAGLGLNLDELVNVTTQSSNQSYCSNVQLPTTEQPFTIDFQTRRR---IGGNGIYN--- 911
                 .:...|.|..:|.:|..:.|.|......:|..::........||..   |...|..|
Zfish   728 KLHFTAQMAAGPNSLQLTDVLGRWSAQEEKDIHKLSRSDGSRLFQNLTRGNWYLIQAEGYLNNSA 792

  Fly   912 AYQKTKMELQHLHNGQL--NAFTFEYLEPYYSGKCTQYSNCLHCLTDASCAWCPLTNICHLRSVN 974
            :.|.::|.|.....|..  :..:|.:|||:.|..|::|.:||.||:|.||.|||..|:|.|||..
Zfish   793 SGQTSEMALTWNRTGLPGGSEISFLFLEPFRSNSCSEYRSCLACLSDQSCGWCPTVNLCFLRSDI 857

  Fly   975 ETEVCKMETLDTFHWSYLISQPSQCSNCTNYVSCEACARSGECEWWTEDAR-----CGRIGKTNS 1034
            ..:.|.....:..|   |:..|..|:.|..|..|.||.:...|||....::     |.|.|:...
Zfish   858 NQQPCIDPQGEERH---LLLNPLHCTLCEEYRDCAACTQDPYCEWQINSSKKGDYLCSRRGRLEG 919

  Fly  1035 SVRAVEHCPRSCRERHGCQECLGERGRCVWCEASAQCFSFSVYTSEYQFGMCREWVDQVVSRQTQ 1099
            |:|....||..|.:|..|.|||....:|.|||::..||.|:.|.:::.:|.||:|.|.|.|
Zfish   920 SIREPGGCPNVCNQRLTCGECLSNSSQCAWCESAQSCFYFAAYLTKFPYGECRDWYDSVHS---- 980

  Fly  1100 EIADHKPQQTPHFLQQQCKSCEQHRNCSSCLRTLSCGWCFDRDNPIEGICMQGDF----SYSAGN 1160
                     .|     |||.|.....||.||:|..||||.|..||..|.|::||:    .:||.|
Zfish   981 ---------VP-----QCKQCTALTTCSECLQTFQCGWCGDYSNPTIGRCLRGDWGGMNDHSAVN 1031

  Fly  1161 CSLAL-------------------------------NSSSHHDAEWAYAQCPDVDECGLGLHDCH 1194
            ||.|:                               ......||.|:|..||||:||.||||.||
Zfish  1032 CSTAVAEVKATSPEPQTLSPPRLMELEMDLEMEQLEGMEGDGDAVWSYPSCPDVEECRLGLHTCH 1096

  Fly  1195 KEAKCTNTQGSYNCHCRRGYIGDGKFSCVRTCYELCQNGNCSGPPDYTCRCALGWT--------- 1250
            ..|.|.||..||.|||.||:.|||...|.:|||..|:.|.|||.|.:.|.|:||||
Zfish  1097 PSATCVNTPTSYECHCERGFTGDGVHHCNQTCYNECRQGRCSGSPLFECECSLGWTSDPATLVLS 1161

  Fly  1251 GADCGLSCGCNNHSTCNERLGKCDQCQDWSEGEKCERCRQGSYGNATA-PHGCLPCECNGHGNQD 1314
            |.:|.:.||||.||||....|.|||||||:.|..||.|:.||:|:|.| ..||:||:|||||:..
Zfish  1162 GVECDVDCGCNFHSTCITAPGICDQCQDWTTGPHCEHCKPGSFGSALARGDGCVPCQCNGHGDPL 1226

  Fly  1315 LGVCNVSNGECYCKDNTQGLNCELCAPGYYGDPRGGGKCYYQCESRGIL----------TNIG-K 1368
            .|.|:...|:|||..:|||.:||.|.||||||||..|.|:.||:.|.:|          :::| .
Zfish  1227 QGFCHNQTGQCYCTHHTQGPHCESCLPGYYGDPRDNGTCFSQCQGRSVLLSSSVPLSLSSSLGWH 1291

  Fly  1369 SAIGSYQSYRSPWGASLEVKECLWILQPKTLQAE----KSLLQLEFQWQ-SLAMDCDENAVYIYD 1428
            .|:|.:.......|.|    .|||:|......|.    :|...:...|. .....|..:.||::|
Zfish  1292 GAVGGHGGVGGHGGLS----HCLWVLSVSQDLAPCMPGQSCPPVALTWHPDSHTHCTNSYVYVFD 1352

  Fly  1429 SLPDLTG---ATQQNQLLAVVC-APYSSPRIIEARSSHVTVHYKQG-SERRHFGFNALYSVMNCV 1488
            .||....   ....:.|:...| .....|..:||.|..|:|:::.. :..|..||||.:.|..|.
Zfish  1353 GLPRFLSNGVVHSHHNLIGAFCGTTRMEPITVEATSGVVSVYFEANVTSGRAQGFNASFWVKRCG 1417

  Fly  1489 AGS-----CISPHICDAQQRCV-----CPAGYVGASCEIEICPSNCNAKRMQGFCDTEYGRCICS 1543
            .||     .||.. |.|..:|:     ||.|..|..|:..:||.:|.|:..:|.|:...|.|:|:
Zfish  1418 NGSTDEGTVISAE-CQAGAQCINGLCECPHGLGGPQCDRPVCPGSCGAEEGRGTCNMNLGLCVCT 1481

  Fly  1544 NANYAGADCGTLVQRNHLVMTELFNTQLLSESLEHLRKTIPRFGHSVNADRRGSLWMFGGYSPNH 1608
            . .:||:||.::...:.||...|.:|||   |:....:.:.|.|||:.:..:|:|||:||.|.:.
Zfish  1482 E-GWAGSDCSSISDTDSLVWETLLDTQL---SVNKAHRFLRRMGHSLMSSPQGTLWMYGGLSLSE 1542

  Fly  1609 GPLNDFRQFDTKNSTWLQVTVESSTPEDRMPLGRYFHASE-IYVK----------KQIIYIYGGI 1662
            |.|.:..::...:..|.:| :.||..|..|||.||.|||. :|.:          :.::::.||:
Zfish  1543 GILGNVYRYSVADRRWTEV-LTSSVEESSMPLPRYHHASALVYYQFPISGSHSATRNLMFVVGGV 1606

  Fly  1663 GANSQLLNDFWMFSIQNQRWSQIKVEVEPPEADYEVDVPPPLAGHTLTHIRYQEHESLILLGGLS 1727
             ....:.||.|..::.:|.|.|           |:..|.||:||||||   .::..|::|:||.|
Zfish  1607 -TKEGVANDTWCLNLDSQEWKQ-----------YKSSVLPPVAGHTLT---VRKGSSVLLIGGYS 1656

  Fly  1728 LNKSRPLELWEFNLDTGRWQQLAAVGARMPVLYGHTSVYHQETNSVYLFGGY-----STEPQSNL 1787
            ........|.||:..:|.|......|.....||||::|||::|::||:||||     :.||...|
Zfish  1657 PENGFNHHLLEFSPQSGNWTIALHTGTPPTGLYGHSAVYHEQTDAVYVFGGYRFHVEAVEPSGEL 1721

  Fly  1788 YALDLQKLSWTEL-PSFRELNSPASLLPRARYFHSAVTTEHYMILYGGRTQPFNGTDVLIAYVYA 1851
            |:|....|:|:.| ||  :.|.|.|     |:||:|...:..|::.||||...:.::.:..|...
Zfish  1722 YSLYYPNLTWSLLVPS--QGNKPLS-----RFFHAAALVKDTMVIVGGRTGEEDYSNTVSLYQIN 1779

  Fly  1852 CNQWVRLTEDVELIGRVPASSYAEDMAIDPDTGAIYVIGGWDGSSTHSHVTKITLPDDICQLWSN 1916
            ||.|:   :.|..:|....||.:..|.:..|  .:::.||::|.:....:| ::||.|.|.|...
Zfish  1780 CNTWI---QPVSSVGEPVNSSVSLAMTVWGD--RLFLSGGFNGVTLGRFLT-LSLPSDPCILLPT 1838

  Fly  1917 GKYQCRHYMG-CSYCTIQNTYSYSSHCFSHGRTPCANHNG------TLVVNNGAACDDDWMASRN 1974
            .: .|....| |.:|                |..||:.:.      ||.:   :.|.....:...
Zfish  1839 PE-ACNSSTGSCVWC----------------RGTCASSDAAERLGCTLGL---STCSPTPRSPDQ 1883

  Fly  1975 CSSFATCGACLAAWP----THQEVAPVCHWCDDCGIRGRCVPAGVDCGRRSAWCNKELSVGVLGL 2035
            |....||..|||..|    :..:.|..|.||.:|. .|.|:.:.|.|.........:..:.:...
Zfish  1884 CRRLKTCSECLARHPKTFSSPVQSALQCKWCTNCP-EGACISSSVSCTSEHDCRINQREIFLSSN 1947

  Fly  2036 CPLPQCYQLSCESCMLQPQCNWARNELGTVE--------------CIAKELVEKNQYRVVES--- 2083
            |....|....|..|....:|.|.|....|.|              |.:..|:..:..:|..|
Zfish  1948 CTETSCEASDCPKCTASGKCMWTRQFKRTGETRRILSVNPTYDWTCFSYALLNVSPMQVESSPPM 2012

  Fly  2084 -CPLPCHTYENCSLCLSQTPTQ-DHQECKWSTMLNLCLTPSSQPLLCAGGVCGLVLEASELQRCP 2146
             ||.|||..:.||.|||...:. ..|.|.||..|..|::||..||.|..|.||.:|  |:...|.
Zfish  2013 PCPEPCHEIKTCSQCLSSRGSDGGWQRCVWSMALQQCMSPSFVPLRCEAGQCGRLL--SDEDSCS 2075

  Fly  2147 EPCHVYTQCSSCLEHAHCGWCAREGFNGDGICTEGALEHKQEHPSGSTCDLIYASWRNDSQLTHA 2211
            ..|...||||.|:....|||||..|.||.|.|.:|.|....|    :.|    ..|.:
Zfish  2076 PQCFQLTQCSQCISRPQCGWCAGRGGNGSGRCLQGGLNGVSE----ALC----PQWNS------- 2125

  Fly  2212 DVVSWHYVQCPAENECINGHHNCDTVSEQCIDLDTAVGYKCVCAQGY--REEQGACLPVCSQGCV 2274
               ||.::.||.|:||.||||:|: |::.|.||..  ||.|.|.|||  ....|.|.|||:||||
Zfish  2126 ---SWSFLHCPEEDECSNGHHHCN-VTQDCHDLPE--GYHCTCRQGYVLSSVSGQCEPVCAQGCV 2184

  Fly  2275 RGNCVSPDQCQCDFGYVGANCSIQCLCNGHSNCESSSRLDICLKCHNNTMGEQCEKCQPLFVGNP 2339
            .|.|:||..|||.||:||.|||.||.||.||||:|.|:||.||:|.|||.|:.|:||:|||||:.
Zfish  2185 NGTCISPGICQCHFGFVGENCSSQCRCNKHSNCKSISQLDTCLECKNNTKGQHCDKCKPLFVGSA 2249

  Fly  2340 REGHACQPCLDYCHGHSDVCVAYD------ADPAVFNMTRSELERILQEGPAY-NATCLRCGNHT 2397
            ..|..|:||.::|.|:||:|::.|      .:|..:.:.|..:|:...|||.. :|.|:.|.|::
Zfish  2250 MGGGMCRPCREFCRGNSDICLSKDEHNKAMENPKDYPLDRESIEQWATEGPTEDHAVCVSCQNNS 2314

  Fly  2398 AGDRCDSCLTGYFRGSEDLHKECRPCQCHGHGNICDPVTGEKCNCANNTESDATCTAGGGKNSAQ 2462
            .||:|:|||:|||.    |..:|..|||:||.:.|:...|..|.|.||||: ::|.: ..:|..:
Zfish  2315 VGDKCESCLSGYFL----LQGKCEKCQCNGHADTCNEHDGTGCPCQNNTET-SSCLS-SPQNDRK 2373

  Fly  2463 LCWMVQCSKCRDSYAGNPTDGHQCYKQITVESRMCFDAKPIEECKSKP--AALKPGQTVFFVIQP 2525
            .|:..||:||::|:.|||.:|.|||:|..|:|..|||......|..:|  ..|..|:||||..||
Zfish  2374 ECYRQQCAKCKESFNGNPVNGRQCYRQFNVDSECCFDPTSQVNCFHEPNIRNLLRGRTVFFSAQP 2438

  Fly  2526 RFMNVDIRIIIDVTQGELDVFMSPQDDSFIVETNETTGYHEIFLDNRYNWGPKIKREHPLNVALP 2590
            :|.|||||:.||||.||::|::|...|:|||:.:..:|.|.|.:::........:.|..:.:|.|
Zfish  2439 KFTNVDIRVTIDVTFGEVEVYVSNSHDTFIVQVDRQSGVHTIKIEDEATSATASRGEKEVPLAPP 2503

  Fly  2591 RHDNVTIQKLFSPERRIGGGGLGGGGGERIGANTYYVPQLQDCKSHGGHNFIVKDQHAKDLSTHV 2655
                 :..|:|:.......|.|..              :||      |....|:::.|:.|.|::
Zfish  2504 -----SPMKVFANSSSALLGTLSA--------------KLQ------GSEREVREERAEGLITYI 2543

  Fly  2656 TLNHCNTLLRLFGLKNRLVLTLPQHAHNLSATRFFIALRASSGPE----PSYGSVVFRQDQLHID 2716
            |:....|:|.:.|:::|:|:|.|...|:|.::||||||| ..|.|    .|.|.:..||||.|||
Zfish  2544 TVWKPQTVLIVRGVRDRVVITFPHEVHSLKSSRFFIALR-GVGTETHNGESQGLLFLRQDQAHID 2607

  Fly  2717 LFVFFSVFFSCFFLFLAVCVIVWKVKQAADLRRARRQHVVEMLHLAKRPFAQIFLASNGLDMDSP 2781
            ||||||||||||||||:|||::|||||..|.||.:|:|:.||..:|.||||::.:.   |:.:.|
Zfish  2608 LFVFFSVFFSCFFLFLSVCVLLWKVKQFLDFRREQRRHIQEMTKMASRPFAKLTVY---LEPEEP 2669

  Fly  2782 Q----PTSSSSSA-------------RAMRQRARQALLLQEQSAGDSQSVMHH------------ 2817
            |    |::...||             ..|.||.|...|..:...|...:..||
Zfish  2670 QLVYLPSAGGGSAVSLAHVRTGKLGGMVMGQRGRPGALSYKHDPGTGTATHHHHHLALSGGNNGQ 2734

  Fly  2818 ---------------STRRQSS----------------------------------RIMMVAIEP 2833
                           ||...:|                                  ::..:.:||
Zfish  2735 HLPLHYLNAHHYAPSSTANPTSHHQHHYPSSHGSYQHFCRSDPFLSQLMGFSYSSFKVGPITLEP 2799

  Fly  2834 TFDNLAAVGTVFISLPGRSRAPLSIALGSTLIS 2866
            |.|.:|.|.||.|.|||...||....|||.|::
Zfish  2800 TDDGMAGVATVLIQLPGGILAPNRACLGSALVT 2832

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Megf8	NP_609180.3	CUB	33..141	CDD:238001	52/107 (49%)
		NanM	<210..455	CDD:442289	59/245 (24%)
		KELCH repeat	228..268	CDD:276965	16/39 (41%)
		KELCH repeat	370..420	CDD:276965	19/49 (39%)
		KELCH repeat	425..476	CDD:276965	24/50 (48%)
		NanM	431..>630	CDD:442289	84/203 (41%)
		KELCH repeat	480..540	CDD:276965	18/61 (30%)
		EGF_3	1186..1222	CDD:463759	21/35 (60%)
		EGF_Lam	1257..1302	CDD:238012	25/45 (56%)
		EGF_Lam	1304..1354	CDD:238012	28/49 (57%)
		KELCH repeat	1585..1638	CDD:276965	18/52 (35%)
		NanM	1596..1892	CDD:442289	99/312 (32%)
		KELCH repeat	1642..1685	CDD:276965	13/53 (25%)
		KELCH repeat	1760..1813	CDD:276965	26/58 (45%)
		KELCH repeat	1817..1867	CDD:276965	14/49 (29%)
		EGF_Lam	2299..2346	CDD:238012	27/46 (59%)
		EGF_Lam	2422..2487	CDD:238012	26/64 (41%)
megf8	XP_005158088.1	CUB	36..134	CDD:238001	50/99 (51%)
		NanM	223..517	CDD:442289	125/401 (31%)
		KELCH repeat	277..326	CDD:276965	19/50 (38%)
		KELCH repeat	329..372	CDD:276965	20/45 (44%)
		KELCH repeat	380..429	CDD:276965	16/48 (33%)
		KELCH repeat	439..487	CDD:276965	28/51 (55%)
		PSI	931..982	CDD:396154	21/63 (33%)
		EGF_3	1088..1122	CDD:463759	21/33 (64%)
		Laminin_EGF	1169..1214	CDD:395007	25/44 (57%)
		EGF_Lam	1216..1265	CDD:238012	28/48 (58%)
		CUB	<1307..1413	CDD:412131	27/109 (25%)
		NanM	1528..1814	CDD:442289	98/313 (31%)
		KELCH repeat	1575..1633	CDD:276965	15/69 (22%)
		KELCH repeat	1636..1676	CDD:276965	15/42 (36%)
		KELCH repeat	1689..1737	CDD:276965	21/47 (45%)
		KELCH repeat	1745..1791	CDD:276965	14/48 (29%)