Protein Alignment Megf8 and atrn-1

DIOPT Version :10

• Sequence 1: NP_609180.3
• Gene: Megf8 / 34099
• FlyBaseID: FBgn0031981
• Length: 2892
• Species: Drosophila melanogaster
• Sequence 2: NP_510443.5
• Gene: atrn-1 / 181566
• WormBaseID: WBGene00006432
• Length: 1269
• Species: Caenorhabditis elegans
• Alignment Length: 1705
• Identity: 356/1705 - (20%)
• Similarity: 550/1705 - (32%)
• Gaps: 659/1705 - (38%)

  Fly  1310 HGN----QDLGVCN--VSNGECYCKDNTQGLNCELCAPGYYGDPRGGGKCYYQCESRGILTNIGK 1368
            |.|    :.|..|:  ..||.|..|      .| :|:.|:||.         ||:.     ..|:
 Worm    31 HANFEFEKSLSSCDKPCYNGVCLNK------AC-VCSKGWYGS---------QCDH-----CFGR 74

  Fly  1369 SAIGSYQSYRS--PWGASLEVKECLWILQPKTLQAEKSLLQLEFQWQSLAMDCDENAVYIYDSLP 1431
            ..|....||.|  |...|...| |.|:::|     |.|...|:.:..|...:|..:.:||||
 Worm    75 IRISDNASYISDGPLDYSPSAK-CTWLIEP-----ENSATPLKIRINSFFTECGWDYLYIYD--- 130

  Fly  1432 DLTGATQQNQLLAVVCAPYSSPRIIEARSSHVTVHYKQGSERRHFGFNALYSVMNCV-----AGS 1491
               |.:...:.||.:|....|.. ..|.|....||:.........|||..|....|.     .||
 Worm   131 ---GDSVYGKQLAALCGEQPSQE-FTAASGKALVHFFSDLAINLNGFNVSYESNRCAYNCSNHGS 191

  Fly  1492 CISPHICDAQQRCVCPAGYVGASCEIEIC-------PSNCNAKRMQGFCDTEYGRCICSNANYAG 1549
            |::       .:|.|..||.|.:||.::|       .|.|:    :|.|..  |||.|.:|...|
 Worm   192 CLN-------GKCDCEDGYKGLNCEYQVCQLSGKSTESPCH----EGQCVD--GRCECLSARVHG 243

  Fly  1550 ADCGTLVQRNHLVMTELFNTQLLSES---LEHLRKTIP--RFGH-SVNADRRGSLWMFGG--YSP 1606
            ..|...|                |.|   |.|.....|  :..| |:..|  ..:|..||  :..
 Worm   244 ETCQMPV----------------SSSVWDLIHPTNNAPTGKASHASIAID--DVVWSIGGEFFDG 290

  Fly  1607 NHGPLNDFRQFDTKNSTWLQVTVESSTPEDRMPLGRYFHASEIYVKKQIIYIYGGIGANSQL--- 1668
            :..| |:...::..:..|.:|.|...     ||..|:.|....|..|  :|::||: ..:|:
 Worm   291 SSDP-NNIDVYNVTSRIWSKVEVSGD-----MPKPRFDHTVVKYKNK--LYMFGGV-TKTQVRHQ 346

  Fly  1669 -----LNDFWMFSIQNQRWSQIKVEVEPPEADYEVDVPPPLAGHTLTHIRYQEHESLILLGGLSL 1728
                 .|:.|:|.:.:::|:|   ::...|.  .:..|..:|||:...||   .|..::.|
 Worm   347 TTQAATNELWIFDMGSKKWAQ---QIHKNET--IIAAPFAVAGHSAHVIR---SEMFVIFG---- 399

  Fly  1729 NKSRPL-----ELWEFNLDTGRWQQLAAVGARMPVLYG---HTSV-YHQET--NSVYLFGG--YS 1780
              ..||     .:..:|.:|..|    .|......:||   |::| |...|  .::.::||  ::
 Worm   400 --YNPLFGFMHHVQIYNFETEEW----TVANTSDHVYGRFKHSAVEYTTPTGATAILVYGGSMWN 458

  Fly  1781 TEPQSNLYALDLQKLSWTELPSFRELNSPASLLPRARYFHSAVTTEHYMILYGGRTQPFNG---- 1841
            .....:|...|.....|:.||     .|...|     |.|:|......|::.|||......
 Worm   459 NTITDSLMQFDTSTKKWSNLP-----QSGVQL-----YLHAAAYLNGLMVVVGGRGSNVTAGSKS 513

  Fly  1842 ---TDVLIAYVYACNQW----------VRLTEDVELIGRVPASSYAEDMAIDPDTGAIYVIGGWD 1893
               ::::.:|..||.||          .|....|.:||:                 .:|.:||::
 Worm   514 ECFSNMVQSYDVACKQWSNMSTAPVDLKRFGHSVHVIGQ-----------------KLYALGGFN 561

  Fly  1894 G-------SSTHSHVTKITLPDDICQLWSNGKYQCRHYMGCSYCTIQNTYSYSSHCFS--HGRTP 1949
            |       :.:.:..:..|.||: |:|.::|. :| .::..|......|.||.|...|  ...||
 Worm   562 GKMKSDVWTLSPAKCSSATRPDE-CRLITDGT-KC-VFVDSSCVPFDPTVSYKSSFASMIKSSTP 623

  Fly  1950 -----CANHNGTLVVNNGAACDDDWMASRNCSSFATCGACLAAWPTHQEVAPVCHWCDDCGIRGR 2009
                 |.|....|             |.:.|.....|.:|.:        ...|.||..   ..:
 Worm   624 KSFDECTNTPLRL-------------ALKTCEEQTDCVSCAS--------KSGCGWCSS---GEQ 664

  Fly  2010 CVPAGVDC----GRRSAW--CNKELSVGVLGLCPLPQCYQLSCESCMLQPQCNWARNELGTVECI 2068
            |:|...:|    |..::|  |.:..||..:..|.:    :.:|.||.:.|.|.|...:..: .|:
 Worm   665 CLPNEQECVDGPGMLTSWEKCPQRNSVATMRPCNM----ENNCGSCRISPHCTWYPIDKAS-PCV 724

  Fly  2069 AKE--------------LVEKNQ----------YRVVESCPLPCHTYENCSLCLSQTPTQDHQEC 2109
            :||              |.::|:          :|....||:||....|||.|      .|.::|
 Worm   725 SKEDLIAVVYEYETKSVLADRNKFLSPSHFPSFFRNATECPMPCAQRNNCSDC------TDLEQC 783

  Fly  2110 KWSTMLNLCLTPSSQPLLCAGGVC------------GLVLEASELQRCPEPCHVYTQCSSCLEHA 2162
            .|....|.|:...:..|..|.|.|            ..|.:|..:     .|..:..|..|....
 Worm   784 MWCPSTNRCINLEAYTLSFAYGQCHSWVTSGSGSVINRVCQAESV-----VCEEHKTCGECQRDP 843

  Fly  2163 HCGWCAREGFNGDGIC----TEGALEHKQEHPSGSTCDLIYASWRNDSQLTHADVVSWHYVQCPA 2223
            .|||.|.:...|.|:|    :.|.||.|   |..||                     |:::.|||
 Worm   844 GCGWLADDSKTGLGLCIRGTSTGPLEPK---PENST---------------------WYFIDCPA 884

  Fly  2224 ENECINGHHNCDTVSEQCIDLDTAVGYKCVCAQGYREEQGACLPVCSQGCVRGNCVSPDQCQCDF 2288
                                                                             
 Worm   885 ----------------------------------------------------------------- 884

  Fly  2289 GYVGANCSIQCLCNGHSNCESS------SRLDICLKCHNNTMGEQCEKCQPLFVGNPREGHACQP 2347
                      |.|||||.|.:|      ..::.|..|.|:|.|..||:|.|.|.|:.|.|..|.
 Worm   885 ----------CQCNGHSTCFTSVGSFPPVTIEKCQSCQNHTTGAHCERCAPGFYGDARNGGVCS- 938

  Fly  2348 CLDYCHGHSDVCVAYDADPAVFNMTRSELERILQEGPAYNATCLRCGNHTAGDRCDSCLTGYFRG 2412
                                                                             
 Worm   939 ----------------------------------------------------------------- 938

  Fly  2413 SEDLHKECRPCQCHGHGNICDPVTGEKCNC-ANNTESDATCTAGGGKNSAQLCWMVQCSKCRDSY 2476
                     ||.||...::||||:|: |.| ......|                  :|.||...|
 Worm   939 ---------PCDCHHQADMCDPVSGQ-CFCKTKGVTGD------------------RCDKCEAKY 975

  Fly  2477 AGNPTDGHQCYKQITVESRMCFDAKPIEECKSKPAALKPGQTVFFVIQPRFMNVDIRIIIDVTQG 2541
            .|||.:|..|:.::.|:                           |:.
 Worm   976 VGNPRNGTPCFYELAVD---------------------------FIF------------------ 995

  Fly  2542 ELDVFMSPQDDSFIVETNETTGYHEIFLDNRYNWGPKIKREHPLNVALPRHDNVTIQ-KLFSPER 2605
               .|....||    :.|.|:   ||:|               .:|...:..:||.| ...||:
 Worm   996 ---TFKLRSDD----KDNHTS---EIYL---------------YSVPYKKDTDVTFQISCESPK- 1034

  Fly  2606 RIGGGGLGGGGGERIGANTYYVPQLQD----------CKSHGGHN-FIVKDQHAKDLSTHVTLNH 2659
                    |.....:...:.||..|.|          |.|.|... ::..|:...
 Worm  1035 --------GNALVALNMTSSYVNGLADKSQAMMVDTICDSKGFRRVYVASDKGYP---------- 1081

  Fly  2660 CNTLLRLFGLKNRLVLTLPQHAHNLSATRFFIALRASSGPEPSYGSVVFRQDQLHIDLFVFFSVF 2724
                   ||         |.     |.|.||:  |..:...|....|.|.|.. .|:..:||.:|
 Worm  1082 -------FG---------PD-----SNTTFFV--RVYNFNTPVQIVVSFAQSP-PINWVLFFVIF 1122

  Fly  2725 FSCFFLFLAVCVIVWKVKQAADLRRARRQHVVEMLHLAKRPFAQIFLASNGLDMDSPQPTSSSSS 2789
            .:||.:.|.|..::|.:|...:..|..::.:.|:.|:|.|||     ||..:::
 Worm  1123 AACFIVLLVVAGLLWMIKVRIEAYRRNQRRIDEIEHMASRPF-----ASTKMEL----------- 1171

  Fly  2790 ARAMRQRARQALLLQEQSAGDSQSVMHHSTRRQSSRIMMVAIEPTFDNLAAVGTVFISLP--GRS 2852
                      ::|.|..|||....               ::|||..:..|.|.|:.:.||  |::
 Worm  1172 ----------SMLSQFSSAGGPTP---------------LSIEPCSNYRAGVFTLAVRLPTGGKA 1211

  Fly  2853 RAP---LSIALGSTL 2864
            ..|   ..:|:.|:|
 Worm  1212 VTPSGTSGLAVASSL 1226

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Megf8	NP_609180.3	CUB	33..141	CDD:238001
		NanM	<210..455	CDD:442289
		KELCH repeat	228..268	CDD:276965
		KELCH repeat	370..420	CDD:276965
		KELCH repeat	425..476	CDD:276965
		NanM	431..>630	CDD:442289
		KELCH repeat	480..540	CDD:276965
		EGF_3	1186..1222	CDD:463759
		EGF_Lam	1257..1302	CDD:238012
		EGF_Lam	1304..1354	CDD:238012	13/49 (27%)
		KELCH repeat	1585..1638	CDD:276965	11/55 (20%)
		NanM	1596..1892	CDD:442289	71/335 (21%)
		KELCH repeat	1642..1685	CDD:276965	12/50 (24%)
		KELCH repeat	1760..1813	CDD:276965	14/60 (23%)
		KELCH repeat	1817..1867	CDD:276965	15/66 (23%)
		EGF_Lam	2299..2346	CDD:238012	21/52 (40%)
		EGF_Lam	2422..2487	CDD:238012	20/65 (31%)
atrn-1	NP_510443.5	CUB	71..179	CDD:238001	33/120 (28%)
		NanM	260..572	CDD:442289	77/367 (21%)
		KELCH repeat	269..316	CDD:276965	11/54 (20%)
		KELCH repeat	320..379	CDD:276965	14/66 (21%)
		KELCH repeat	382..422	CDD:276965	12/52 (23%)
		KELCH repeat	485..537	CDD:276965	13/56 (23%)
		PSI	640..686	CDD:396154	11/56 (20%)
		PSI	767..816	CDD:396154	15/54 (28%)
		PSI	830..883	CDD:396154	18/76 (24%)
		EGF_Lam	884..937	CDD:238012	22/127 (17%)
		EGF_Lam	939..985	CDD:238012	20/64 (31%)