Protein Alignment Megf8 and atrnl1

DIOPT Version :10

• Sequence 1: NP_609180.3
• Gene: Megf8 / 34099
• FlyBaseID: FBgn0031981
• Length: 2892
• Species: Drosophila melanogaster
• Sequence 2: XP_031761586.1
• Gene: atrnl1 / 100495547
• XenbaseID: XB-GENE-6033278
• Length: 1405
• Species: Xenopus tropicalis
• Alignment Length: 1802
• Identity: 390/1802 - (21%)
• Similarity: 610/1802 - (33%)
• Gaps: 609/1802 - (33%)

  Fly  1285 CERC------RQG----------SYGNATAPHGCLPCECNGHGNQDLGVCNVSNGECY---CKDN 1330
            |..|      |.|          |.|.|        ||.|               .|:   |.:|
 Frog    10 CHNCPGMSLLRSGLFYLLIFAYSSQGRA--------CEKN---------------PCFSGRCVNN 51

  Fly  1331 TQGLNCELCAPGYYGDPRGGGKCYYQCESR-------GILTNIGKSAIGSYQSYRSPWGASLEVK 1388
            |    | ||.||:.|:     .|.: |:.|       |.||:             .|.....:.|
 Frog    52 T----C-LCDPGWVGE-----LCQH-CQGRFRLTEPSGYLTD-------------GPINYKYKTK 92

  Fly  1389 ECLWILQ--PKTLQAEKSLLQLEFQWQSLAMDCDENAVYIYDSLPDLTGATQQNQLLAVVC---- 1447
             |.|:::  |      .::|:|.|  ...|.:|..:.:||||      |.:..:.|:||:.
 Frog    93 -CTWLIEGYP------HAVLRLRF--NHFATECSWDHMYIYD------GDSIYSPLIAVLSGLIV 142

  Fly  1448 --APYSS--PRIIEARSSHVTVHYKQGSERRHFGFNALYSVMNCVA-----GSCISPHICDAQQR 1503
              ||.:.  |.:: ..|....:|:...:.....|||..||:.:|.:     |.|....:..::..
 Frog   143 PEAPGNETVPEVV-TTSGFALLHFFSDAAYNLTGFNIFYSINSCPSNCSGHGKCTGSSLVPSRVY 206

  Fly  1504 CVCPAGYVGASCEIEICPSNCNAKRMQGFCD-TEYGRCICSNANYAGADCGTLVQRNHLVMTELF 1567
            |.|...:.|.:|:|..|.:||.:.. .|:|| |....|:| |.::.|.||...|...
 Frog   207 CECDKYWKGEACDIPYCKNNCGSPD-HGYCDLTGEKLCVC-NDSWQGPDCSLTVPST-------- 261

  Fly  1568 NTQLLSESLEHLRKTIPRFGHSVNADRRGS-LWMFGGYSPNHGPLNDFRQFDTKNSTWLQVTVES 1631
            ::..:..:::....::.|..|  .|...|. :|:.||::.|:........::.::|.|      :
 Frog   262 DSYWILPNVKPFSPSVGRASH--KAVMHGKFMWVIGGHAFNYSSFQMVLNYNVESSIW------N 318

  Fly  1632 STPEDRMPLGRYFHASEIYVKKQIIYIYGG----IGANSQLLNDFWMFSIQNQRWSQIKVEVEPP 1692
            ..|..|.||.||.|:..:|  :..||:|||    ||.|  :.::.|:|:|.:|.||.....|...
 Frog   319 VVPVLRGPLQRYGHSLALY--QDDIYMYGGKIETIGGN--VTDELWVFNISSQSWSTRTPTVLNH 379

  Fly  1693 EADYEVDVPPPLAGHTLTHIRYQEHES-LILLGGLSLNKSRPLELWEFNLDTGRWQQLAAVGARM 1756
            ...|.|:      |||...:.....:: ::::.|.|........:.||.|.:..|......||.:
 Frog   380 GQHYAVE------GHTAHIVELDNRDAVMVIIFGYSSVYGYISNVQEFYLKSNTWLVPETRGAIV 438

  Fly  1757 PVLYGHTSVYHQETNSVYLFGGYSTEPQS------NLYALDLQKLSWTELPSFRELNSPASLLPR 1815
            ...|||||||...|.::|:.|||...|.:      :||..::...:||.|       ..:.|
 Frog   439 QGGYGHTSVYEPITKAIYIHGGYKALPGNKYGLVDDLYRYEVNTRTWTIL-------KESGL--- 493

  Fly  1816 ARYFHSAVTTEHYMILYGGRTQPFNGTDV----------LIAYVYACNQW-----VRLTEDVELI 1865
            :||.|:||.....|:::||.|.  |.|.:          .:||..||::|     ..|..||...
 Frog   494 SRYLHTAVLISGTMLVFGGNTH--NDTALSNGAKCFSADFLAYDIACDEWRVLQKPNLHRDVNRF 556

  Fly  1866 GRVPASSYAEDMAIDPDTGAIYVIGGWDGSSTHSHVTKITLPDDI-------CQLWSNGKYQC-- 1921
            |.....|          .|::|:.||:..          .|.:||       |:.: ..:.||
 Frog   557 GHSAVVS----------NGSMYIFGGFSS----------VLLNDILVYKPPSCEAF-RSEDQCTS 600

  Fly  1922 -RHYMGC----SYCTIQNTYSYSSHCFSHGRTPCANHNGTLVVNNGAACDDDWMASRNCSSFATC 1981
             ...:.|    .:|....:...|.|.|   |..|.        |..||.||      .|.....|
 Frog   601 AARGISCLWDKDHCVSWESVRPSRHLF---RANCR--------NKSAAADD------RCYKNTDC 648

  Fly  1982 GACLAAWPTHQEVAPVCHWCDDCGIRGRCVPAGVDCG-----------RRSAWCNKELSVGVLGL 2035
            .:|.|.       ...|.||||    .:||....:|.           |....|||
 Frog   649 ASCTAN-------TNGCQWCDD----KKCVSLYSNCSVSVKNYTKCYVRNEQICNK--------- 693

  Fly  2036 CPLPQCYQLSCESCMLQPQCNWARNELGTVEC--IAKELVEKNQYRVVESC-------------P 2085
                   ..||:||.|...|.|.:.:   .||  :...|..:....|.:||             .
 Frog   694 -------LTSCKSCSLNLNCQWDQRQ---QECQALPAHLCGEGWSHVGDSCLRINSSRDSYDNAR 748

  Fly  2086 LPCH----------TYENCSLCLSQTPTQDHQECK--------------WSTMLNLCLT------ 2120
            :.||          |.:.....|.:.....:|:..              |..|.....|
 Frog   749 VYCHNLSGSLASLTTSKEVDFVLDEMQKYTNQKLSPWVGLRKINVSYWGWEDMCPFTNTTLQWLP 813

  Fly  2121 --PSSQPLL---------------CAGGVCGLVLEA------SELQRCPEPCHVYTQCSSCLEHA 2162
              ||.....               |.....|||.|.      ...:.|..||.:.|.||:|..:.
 Frog   814 GEPSDSGFCAYLERAEVAGLKANPCTAMTDGLVCEKPVVSPNQNARPCKLPCSLRTSCSNCTSNG 878

  Fly  2163 -HCGWCAREGFNGDGICTEGALEHKQEHPSGSTCDLIYASWRNDSQLTHADVVSWHYVQCPAENE 2226
             .|.||:..     ..|.:            |...:|        ...:...:.|....|.::|
 Frog   879 MECMWCSNA-----KRCVD------------SNAYII--------SFPYGQCLEWQTTTCSSQN- 917

  Fly  2227 CINGHHNCDTVSEQCIDLDTAVGYKCVCAQGYREEQGACLPVCSQGCVR-------------GNC 2278
            | :|...|    .||:: ....|:   |.......:|:|:...|:|.:|             |.|
 Frog   918 C-SGFRTC----AQCLE-QPGCGW---CNDPSNTGKGSCVEGSSRGPMRPLGKHSSEMVLDTGLC 973

  Fly  2279 VSPDQCQCDFGYVGANCSIQCLCNGHSNCESSSRLDICLKCHNNTMGEQCEKCQPLFVGNPREGH 2343
              |.:...::.::  :|. .|.|||||.|.::   ::|.:|.|.|||:.||.|.|.:.|||..|.
 Frog   974 --PREKNYEWSFI--HCP-ACQCNGHSTCVNT---NVCEQCKNLTMGKHCESCMPGYYGNPTNGG 1030

  Fly  2344 ACQP---CLDYCHGHSDVCVAYDADPAVFNMTRSELERILQEGPAYNATCLRCGNHTAGDRCDSC 2405
            .|:.   |.|||  .:.|....|..|.:.....|:|..:  ..|.|
 Frog  1031 ECEAMLLCTDYC--GAGVMAEMDEQPGILFPLHSKLSSL--TAPLY------------------- 1072

  Fly  2406 LTGYFRGSEDLHKECRPCQCHGHGNICDPVTGEKCNCANNTESDATCTAGGGKNSAQLCWMVQCS 2470
                      :..|..|           |:                ||                .
 Frog  1073 ----------VRPETPP-----------PI----------------CT----------------R 1084

  Fly  2471 KCRDSYAGNPTDGHQCYKQITVESRMCFDAKPIEECKSKPAALKPGQTVFFVIQPR--FMNVDIR 2533
            :.||            |....::.:..|.....|:        |....:.|:..|.  ||.:.:.
 Frog  1085 EMRD------------YTAFLIDYQFTFSLVQEED--------KYHTAINFIANPEQMFMVICVP 1129

  Fly  2534 IIIDVTQGELDVFMSPQDDSFIVE-------TNETTGYHEI--FLDNRYNWGPKIKREHPLNVAL 2589
            |:               .||.:::       ..|...||..  |:.|     |:...:: |::::
 Frog  1130 IL---------------TDSLLIDYQFTFSLVQEEDKYHTAINFIAN-----PEQSNKN-LDISI 1173

  Fly  2590 PRHDNVTIQKLFSPERRIGGGGLGGGGGERIGANTYYVPQLQDCKSHGGHNFIVKDQHAKDLSTH 2654
            ...:|..:...:|         :|...|...|..   ||             :|...|.|:....
 Frog  1174 NASNNFNLNITWS---------IGSTAGTISGEE---VP-------------LVSRAHIKEYRDS 1213

  Fly  2655 VTLNHCNTLLRLFGLKNRLVLTLPQHAHNLSATRFFIALRASSGPEPSYGSVVFRQDQLHIDLFV 2719
            .:   |..    |..::...:|...:..|.:              .|....:.|.|....:||..
 Frog  1214 FS---CEK----FNFRSNPNITFYVYVSNFT--------------WPIKIQIAFSQHNTIMDLVQ 1257

  Fly  2720 FFSVFFSCFFLFLAVCVIVWKVKQAADLRRARRQHVVEMLHLAKRPFAQIFLASNGLDMDSPQPT 2784
            ||..|||||...|.|..:|||:||.....|.|.|.:.|...:|.||||.:.:
 Frog  1258 FFVTFFSCFLSLLLVAAVVWKIKQTCWASRRREQLMRERQQMASRPFATVDV------------- 1309

  Fly  2785 SSSSSARAMRQRARQALLLQEQSAGDSQSVMHHSTRRQSSRIMMVAIEPTFDNLAAVGTVFISLP 2849
                   |:.....|..:|:..:.|..:.               :||||...|.|||.|||:.||
 Frog  1310 -------ALEVGPEQGDILRAPTEGAPKP---------------IAIEPCSGNKAAVLTVFLCLP 1352

  Fly  2850 -GRSRAP----LSIALGSTLISYSRQYPLNTRHFMRAQRGQNVANHP 2891
             |.|..|    ..:|:.|.||..|:|..|:.:.  ::...:|...||
 Frog  1353 RGLSGVPPPGQSGLAIASALIDISQQKSLDCKD--KSSCIRNRKQHP 1397

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Megf8	NP_609180.3	CUB	33..141	CDD:238001
		NanM	<210..455	CDD:442289
		KELCH repeat	228..268	CDD:276965
		KELCH repeat	370..420	CDD:276965
		KELCH repeat	425..476	CDD:276965
		NanM	431..>630	CDD:442289
		KELCH repeat	480..540	CDD:276965
		EGF_3	1186..1222	CDD:463759
		EGF_Lam	1257..1302	CDD:238012	7/32 (22%)
		EGF_Lam	1304..1354	CDD:238012	13/52 (25%)
		KELCH repeat	1585..1638	CDD:276965	11/53 (21%)
		NanM	1596..1892	CDD:442289	86/322 (27%)
		KELCH repeat	1642..1685	CDD:276965	18/46 (39%)
		KELCH repeat	1760..1813	CDD:276965	18/58 (31%)
		KELCH repeat	1817..1867	CDD:276965	19/64 (30%)
		EGF_Lam	2299..2346	CDD:238012	21/46 (46%)
		EGF_Lam	2422..2487	CDD:238012	6/64 (9%)
atrnl1	XP_031761586.1	CUB	67..182	CDD:238001	32/143 (22%)
		DSL	<208..254	CDD:473190	15/47 (32%)
		NanM	277..572	CDD:442289	88/334 (26%)
		KELCH repeat	279..324	CDD:276965	11/52 (21%)
		KELCH repeat	329..377	CDD:276965	18/51 (35%)
		KELCH repeat	385..431	CDD:276965	10/51 (20%)
		KELCH repeat	495..550	CDD:276965	16/56 (29%)
		CLECT_attractin_like	723..849	CDD:153067	17/125 (14%)
		PSI	864..910	CDD:396154	13/70 (19%)
		EGF_Lam	988..1033	CDD:238012	21/47 (45%)