DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Rca1 and FBXO5

DIOPT Version :9

Sequence 1:	NP_477214.1	Gene:	Rca1 / 33959	FlyBaseID:	FBgn0017551	Length:	411	Species:	Drosophila melanogaster
Sequence 2:	NP_036309.1	Gene:	FBXO5 / 26271	HGNCID:	13584	Length:	447	Species:	Homo sapiens

Alignment Length:	415	Identity:	85/415 - (20%)
Similarity:	136/415 - (32%)	Gaps:	134/415 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    47 LEDAEGENCRNASNTTTFFRGLNTPSGHQEQDLYWGKPYPRTQPQKKFSAEEEPFSMTPRLQDEH 111
            |.:.|.::.:...|:|.....|.|      ..||....|.....|...|..||...:.....|
Human   115 LHNKENQHVQQTLNSTNEIEALET------SRLYEDSGYSSFSLQSGLSEHEEGSLLEENFGD-- 171

  Fly   112 SLPKRRKKHFQSPHSSPKKSKKLLFP--HIEE------------PPK-----------------N 145
            || :......|||...|.|:   |.|  |.|:            .||                 .
Human   172 SL-QSCLLQIQSPDQYPNKN---LLPVLHFEKVVCSTLKKNAKRNPKVDREMLKEIIARGNFRLQ 232

  Fly   146 RFYG---GVEKLDIVAKLAQWQPALQCILRHVGAHTLDV----MTKVSPAWKQAVYRSQRDLERL 203
            ...|   |:|.:||:::|  ::..|:.:|..:.|...|:    ::|||..||: :....:...:|
Human   233 NIIGRKMGLECVDILSEL--FRRGLRHVLATILAQLSDMDLINVSKVSTTWKK-ILEDDKGAFQL 294

  Fly   204 QN---HRLKLNLTKENPHVPKR--------CSHVPKANHTVPLQTSNHSSLANSAASLMDSGNS- 256
            .:   .|:..|..|.:||...|        .:.|.|:.....|:....:.|:|..    |...|
Human   295 YSKAIQRVTENNNKFSPHASTREYVMFRTPLASVQKSAAQTSLKKDAQTKLSNQG----DQKGST 355

  Fly   257 -SIHLMDVDAGRVLREQTQRVKCPRCGRGSRVFISEAAKCGENLLSQTLPIGRTTSTFPCMTGPP 320
             |.|....:..:.|::......|.||                          .:.:.:.|.
Human   356 YSRHNEFSEVAKTLKKNESLKACIRC--------------------------NSPAKYDCY---- 390

  Fly   321 LKRFLSLDLDEVRTSPQGPPYNFAECTSVICQFRFCVNCLCKSHPGERCLVTELDTPSKLMMPRE 385
            |:|                    |.|....|.|.:|..|||..|..:.|      :..||:....
Human   391 LQR--------------------ATCKREGCGFDYCTKCLCNYHTTKDC------SDGKLLKASC 429

  Fly   386 RLTP-PQRAQNRDPKITRKNSLKRL 409
            ::.| |      ..|.::|| |:||
Human   430 KIGPLP------GTKKSKKN-LRRL 447

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	Identity
Rca1	NP_477214.1	None
FBXO5	NP_036309.1	Interaction with EVI5. /evidence=ECO:0000269\|PubMed:16439210	135..244	26/120 (22%)
		Requires for efficient binding to CDC20. /evidence=ECO:0000250\|UniProtKB:Q7TSG3	261..409	35/202 (17%)
		Sufficient for interaction with RPS6KA2, Prevents association of CDC20 with RPS6KA2. /evidence=ECO:0000250\|UniProtKB:Q7TSG3	261..339	17/78 (22%)
		Inhibits APC ubiquitin ligase activity. /evidence=ECO:0000269\|PubMed:23708605	305..447	39/208 (19%)
		Competitively blocks access of APC substrates to the D-box coreceptor formed by FZR1 and ANAPC10. /evidence=ECO:0000269\|PubMed:23708001	322..325	0/2 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	337..358	5/24 (21%)
		Allows a rapid multiple mono-ubiquitination of the APC substrate, but strongly inhibits the slow ubiquitin chain elongation catalyzed by UBCH10. /evidence=ECO:0000269\|PubMed:23708001	378..420	16/97 (16%)
		Sufficient to suppress UBE2S activity, essential for interaction with UBE2S, competitively inhibits the rapide ubiquitin chain elongation by UBE2D1 which blocks UBE2D1 with APC, indispensable for recruitment and position of FBXO5 to the catalytic site of APC, abrogates the inhibition of ubiquitin chain assembly primarily catalyzed by UBE2S, inhibits the ubiquitination by either UBE2C or UBE2D1. /evidence=ECO:0000269\|PubMed:23708001	437..447	4/10 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165159652
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR15493
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	3	2.990