DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tsp and thbs4a

DIOPT Version :9

Sequence 1:	NP_001137802.1	Gene:	Tsp / 33941	FlyBaseID:	FBgn0031850	Length:	1060	Species:	Drosophila melanogaster
Sequence 2:	NP_001107896.2	Gene:	thbs4a / 563429	ZFINID:	ZDB-GENE-080215-11	Length:	950	Species:	Danio rerio

Alignment Length:	1080	Identity:	419/1080 - (38%)
Similarity:	567/1080 - (52%)	Gaps:	167/1080 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 WTRVLL-IGLTALALTFVDVASLSLDPVASAELEQFIRKGDV---------VISTRHIR---PRR 54
            ||||:. :.|....|..|....:..|.:.|.:....:.:|.:         :::|..::   |.|
Zfish     4 WTRVIFSLQLILQLLDRVKSQGIVYDLLVSPDCLPDLLQGGIKAKNMDNVFILATLQMQSKTPTR 68

  Fly    55 KLHISIEALFMIDFPMLKHKMSFFLDRKQQRVTLD-ISANGATESRNF-EIP---NINETSTIRS 114
            ..|| ..||...|:      ..|.:..|..||.|. :.:||...:..| ::|   ..:....:..
Zfish    69 VFHI-FNALDNTDY------FDFTVQGKLNRVILKYLKSNGKVGTTTFNKVPLTDGQHHHILLHF 126

  Fly   115 LALQFSKNRITLHVDCKASTHHDIDMNLAKLYTQMDDPVIKLFRERKYPLHFDGDMEHSLQRANC 179
            ..||...:...|:|||:                 :.|.|:.:      ||.|     .||.
Zfish   127 SGLQQGSSATALYVDCR-----------------LVDRVLDV------PLAF-----KSLP---- 159

  Fly   180 QKGNHRRGNRRMLRNKITEREKNKKRDVRGWYEPTIAREGVVDHRHQEVPTDV-ERGDIPVLNGD 243
                  :|.:|:....:....::...|::...:.:|  :.||:.:.....::: |:.:...|.|.
Zfish   160 ------QGPKRVTLKTLPTSTQDTLSDLKLVLDESI--DNVVELQDCSTLSEMTEQKETLQLLGT 216

  Fly   244 CEDALARSLSDLLALVKLLREDVAHQRQEIAYLRMLLENCAGCKNPLTTDNQLRIEPDCRSANPC 308
            .....|..:.:|:.:::.|::.|..|.:|.|.|:..:..|..| |.|.......::    :..|
Zfish   217 TSKVEAGPMFELMRMIQELKQKVDQQTKETADLKKTILECVPC-NGLGGQTHAGLQ----NKKP- 275

  Fly   309 YPGVECLDSAAGPRCGHCPLGFIGDGKSCKPGVTCAHHMCYPGVQCHDTVNGAQCDSCPAGYEGD 373
                                      .:|.|||      |:....|.:..:|..|..||.||.||
Zfish   276 --------------------------SNCGPGV------CFTQDLCVEKADGVDCAPCPEGYTGD 308

  Fly   374 GRTCSLRNPCLDTPCPSGAQCLQVGYPPYFHCISCPMGHEVNGTSCRDMNECLLYDPCDELATCT 438
                                                      |..|.|::|| .::||.....|.
Zfish   309 ------------------------------------------GVHCEDVDEC-QFNPCFFGVRCV 330

  Fly   439 NLSPGFQCSPCPVGFDGTHAHGYFADYYSVDYRRQTCLDVDECRTGFFRCPEHSTCINEIGSYRC 503
            |.:|||:|..||.||.|....|...:|...:  :|.|.|:|||......|..:|.|.|.:|::.|
Zfish   331 NTAPGFRCEQCPRGFSGPEVQGVGVNYAQTN--KQICNDIDECLVDNGGCTPNSNCHNTMGAFHC 393

  Fly   504 -QCHEGYVTNGTYSC----------LDRSSVFMCPDG--TVCDRNAVCLRMDNIRHKCHCNVGWA 555
             .|..|:..:....|          :||    :|.:|  ..||.||.|:...:....|.|.:|||
Zfish   394 GSCKAGFTGDQNSGCKTAGPDAVPPMDR----LCGNGQPNPCDINAECVVERDGSISCVCKIGWA 454

  Fly   556 GNGLICGRDTDVDGWPDQAIGCPELRCQRDNCPKLPNSGQEDADLDGHGDGCDDDADGDNVQNSQ 620
            |||.:||:|||:|.:||..:.|.:..|::|||..:|||||||||.||.||.||:|||.|.:.|.|
Zfish   455 GNGYVCGKDTDIDAYPDDILKCRDATCRKDNCMFVPNSGQEDADRDGKGDACDEDADSDGILNDQ 519

  Fly   621 DNCWLAYNTEQLDSDGDKVGDVCDNCVLKYNPRQLDTDEDGLGDECDGDIDNDSIPNALDNCPLL 685
            |||||..|.:|.:||.|..||.||||:...||.|.|||.|||||.||.|||.|.:.|.||||..:
Zfish   520 DNCWLTPNIDQKNSDKDLHGDACDNCIEIKNPDQRDTDNDGLGDACDDDIDGDGLKNILDNCQKV 584

  Fly   686 PNPSQSDVDNDGVGDACDNCPNLPNPDQKDRDMDFVGDACHRDIDGDDDGVPNSLDNCPMVSNSD 750
            |||.|.|.|:||||||||:||::|||:|.|.|.|.|||.|..:||.|.||..|:.||||.|.||:
Zfish   585 PNPDQKDKDHDGVGDACDSCPDVPNPNQSDIDNDLVGDTCDTNIDSDGDGHQNTKDNCPNVINSE 649

  Fly   751 QLDTDGDGTGDECDDDMDGDGIPNYKDNCPLAKNPKQDDFNRNGKGDSCEDDEDVDGVPNGMDNC 815
            |||||.||.|||||||.|.|||.:..|||.|..||.|.|.|.:|.||.|..|.|.|.|.:.:|.|
Zfish   650 QLDTDKDGLGDECDDDDDNDGILDDNDNCRLVPNPDQKDENNDGVGDVCTGDFDKDHVIDRIDTC 714

  Fly   816 PNNSMIHHTDFRTLQTIPLDPKGLSQADPNWVVHANGTEIVQTLNSDPGLAVGKDAFGGVDFDGT 880
            |.|:.|..||||..||:.|||:|.:|.||||||...|.|||||:|||||||||..||.||||:||
Zfish   715 PENAEITLTDFRAYQTVVLDPEGEAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGT 779

  Fly   881 FYINDDTDDDYAGFVFSYQSSYKYYVVQWKKGTQTYWEPRPFTASAAPGIQIKLVNSTEGPGPMM 945
            |::|..||||||||:||||.|..:|||.||:..||||:..||.|.|.||||:|.|.|..|||..:
Zfish   780 FHVNTVTDDDYAGFIFSYQDSSSFYVVMWKQTEQTYWQAVPFRAVAEPGIQLKAVKSNTGPGEFL 844

  Fly   946 RNSLWHEGNTDGEARLLWKDPKNIAWKERTSYRWSLVHRPAIGLIRLQMHEGNRLIFDSGNVFDS 1010
            ||||||.|:|..:.|||||||:|:.||::.||||:|.|||.:|.||:|.:||..|:.|||.|.|:
Zfish   845 RNSLWHTGDTKEQVRLLWKDPRNVGWKDKVSYRWNLKHRPQVGYIRVQFYEGTDLVADSGVVIDT 909

  Fly  1011 TLKGGRLGVFCFSQRMIIWSNLQYKCNNRVKPLIYNDLSD 1050
            :::|||||||||||..||||||:|:||:.: |..|.:..|
Zfish   910 SMRGGRLGVFCFSQENIIWSNLRYRCNDTI-PEYYIEEQD 948

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tsp	NP_001137802.1	EGF_CA	477..513	CDD:284955	12/36 (33%)
		TSP3 repeat_1C	563..595	CDD:275367	14/31 (45%)
		TSP3 repeat_short	632..654	CDD:275365	11/21 (52%)
		TSP_3	656..690	CDD:280556	22/33 (67%)
		TSP3 repeat_long	656..690	CDD:275366	22/33 (67%)
		TSP3 repeat_short	691..713	CDD:275366	15/21 (71%)
		TSP3 repeat_long	714..751	CDD:275365	20/36 (56%)
		TSP_3	715..751	CDD:280556	20/35 (57%)
		TSP3 repeat_short	752..787	CDD:275366	23/34 (68%)
		TSP_3	789..822	CDD:280556	14/32 (44%)
		TSP_C	841..1038	CDD:283408	125/196 (64%)
		coiled coil	245..286	CDD:293927	9/40 (23%)
		TSPcc_insect	245..286	CDD:293927	9/40 (23%)
		EGF_CA	343..377	CDD:214542	10/33 (30%)
		EGF_CA	421..457	CDD:238011	16/35 (46%)
thbs4a	NP_001107896.2	LamG	<54..186	CDD:304605	33/176 (19%)
		coiled coil	220..259	CDD:293923	9/38 (24%)
		TSPcc	220..259	CDD:293923	9/38 (24%)
		EGF_CA	314..348	CDD:238011	15/34 (44%)
		EGF_3	371..403	CDD:289699	9/31 (29%)
		EGF_3	423..460	CDD:289699	15/36 (42%)
		TSP3 repeat_long	495..530	CDD:275366	22/34 (65%)
		TSP_3	496..530	CDD:280556	21/33 (64%)
		TSP3 repeat_short	531..553	CDD:275366	11/21 (52%)
		TSP3 repeat_long	554..589	CDD:275366	22/34 (65%)
		TSP3 repeat_short	590..612	CDD:275365	15/21 (71%)
		TSP3 repeat_long	613..650	CDD:275365	20/36 (56%)
		TSP_3	614..650	CDD:280556	20/35 (57%)
		TSP_3	688..721	CDD:280556	14/32 (44%)
		TSP_C	740..937	CDD:283408	125/196 (64%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170592968
Domainoid	1	1.000	285	1.000	Domainoid score	I1574
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	738	1.000	Inparanoid score	I580
OMA	1	1.010	-	-		QHG49685
OrthoDB	1	1.010	-	-		D120983at2759
OrthoFinder	1	1.000	-	-		FOG0001167
OrthoInspector	1	1.000	-	-		otm26215
orthoMCL	1	0.900	-	-		OOG6_101187
Panther	1	1.100	-	-	O	PTHR10199
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X995
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.910