DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tsp and thbs3a

DIOPT Version :9

Sequence 1:	NP_001137802.1	Gene:	Tsp / 33941	FlyBaseID:	FBgn0031850	Length:	1060	Species:	Drosophila melanogaster
Sequence 2:	NP_775332.1	Gene:	thbs3a / 252849	ZFINID:	ZDB-GENE-020708-3	Length:	962	Species:	Danio rerio

Alignment Length:	1091	Identity:	418/1091 - (38%)
Similarity:	562/1091 - (51%)	Gaps:	215/1091 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 LIGLTALALTFVDVASLSLDPVASAELEQFIRKGDVVISTRHIRPR-------------RKLHIS 59
            ::||..:..|...|..|||.....:::        .|:||..:.|:             .|.::.
Zfish    34 VLGLEDVKQTVAAVEKLSLALKTLSDV--------YVMSTFRLPPKLGGVLLGLYNKQDNKKYLE 90

  Fly    60 IEALFMIDFPMLKHKMSFFLDRKQQRVTLDISANGATESRNFEIPNINETSTIRSLALQFSKNR- 123
            :..:..|:..::::                :..:|...:.|.:.||:.: ...:||.|:....|
Zfish    91 VAIMSKINKVLVRY----------------VREDGKLHTVNMQSPNVAD-GRPQSLILRVGGLRR 138

  Fly   124 ----ITLHVDCKASTHHDIDMNLAKLYTQMDDPVIKLFRERKYPLHFDGDMEHSLQRANCQKGNH 184
                :.|:|:|:.:..           .|...|::.|.|:.:.           ::..|..|...
Zfish   139 EYLSLELYVNCRLADS-----------AQRLPPLVDLPRDAEL-----------VEIRNGHKAYA 181

  Fly   185 R-RGNRRMLRNKITEREKNKKRDVRGWYEPTIAREGVVDHRHQEVPTD-----------VERGDI 237
            | :|:...|           |..:.|    |:|:.|.:        ||           :..|::
Zfish   182 RMQGSMDTL-----------KLALGG----TVAQAGAL--------TDCPFQGDASSYNIVNGEV 223

  Fly   238 PVLNGDCEDALARSLSDLLALVKLLREDVAHQRQEIAYLRMLLENCAGC--KNPLTTDNQLRIEP 300
            ..:.||...||...|.....::..||||:..|.:|::.:|..:..|..|  ..|     :.|.:|
Zfish   224 NSILGDHTKALIGQLIIFNQILGELREDIREQVKEMSLVRNAILECQMCGFHEP-----RSRCQP 283

  Fly   301 DCRSANPCYPGVECLDSAAGPRCGHCPLGFIGDGKSCKPGVTCAHHMCYPGVQCHDTVNGAQCDS 365
                 |||:.||.|:::..                             |||.:|
Zfish   284 -----NPCFKGVSCMETFE-----------------------------YPGYRC----------- 303

  Fly   366 CPAGYEGDGRTCSLRNPCLDTPCPSGAQCLQVGYPPYFHCISCPMGHEVNGTSCRDMNECLLYDP 430
                                .|||.|    ..|                |||.|:|::||....|
Zfish   304 --------------------GPCPDG----MTG----------------NGTHCQDIDECSEAQP 328

  Fly   431 CDELATCTNLSPGFQCSPCPVGFDGTHAHGYFADYYSVDYRRQTCLDVDECRTGFFRCPEHSTCI 495
            |.....|.|.:.||.|..||.|..|....|...:|  ....||.|.|:|||......|..:|.||
Zfish   329 CYTPGACVNTARGFTCESCPPGMWGPPLSGVGVEY--AKSHRQECSDIDECVDLANACTPNSVCI 391

  Fly   496 NEIGSYRC-QCHEGYVTNGTYSCLDR---SSVFMCPDGTVCDRNAVCLRMDNIRHKCHCNVGWAG 556
            |.|||:|| ||..|||.|.|..|..|   ||:...|    ||.||.|:...|....|.|||||||
Zfish   392 NTIGSFRCGQCKTGYVGNQTAGCFPRKSCSSLSFNP----CDTNAHCVMQRNGDVSCACNVGWAG 452

  Fly   557 NGLICGRDTDVDGWPDQAIGCPE--LRCQRDNCPKLPNSGQEDADLDGHGDGCDDDADGDNVQNS 619
            ||..||:|||:||:||:::.|.:  ..|::|||...|||||||||.||.||.||:|||||.::|.
Zfish   453 NGHTCGKDTDIDGYPDRSLPCMDNHKHCRQDNCVYTPNSGQEDADNDGIGDQCDEDADGDGIKNV 517

  Fly   620 QDNCWLAYNTEQLDSDGDKVGDVCDNCVLKYNPRQLDTDEDGLGDECDGDIDNDSIPNALDNCPL 684
            :|||.|..|.:|.:||.|..||.||||....|..|.|||.:|.||.||.|||.|.|.|.|||||.
Zfish   518 EDNCRLVSNKDQQNSDTDSFGDACDNCPTVPNIDQKDTDSNGEGDACDDDIDGDGIQNVLDNCPK 582

  Fly   685 LPNPSQSDVDNDGVGDACDNCPNLPNPDQKDRDMDFVGDACHRDIDGDDDGVPNSLDNCPMVSNS 749
            :|||.|:|.|.||||||||:||.:.||.|.|.|.|.|||.|..:.|.|.||..::.||||.:.||
Zfish   583 VPNPMQTDRDRDGVGDACDSCPEISNPMQTDVDNDLVGDVCDTNQDTDGDGHQDTRDNCPDIPNS 647

  Fly   750 DQLDTDGDGTGDECDDDMDGDGIPNY-------KDNCPLAKNPKQDDFNRNGKGDSCEDDEDVDG 807
            .|||:|.||.||:||:|.|.||||:.       .|||.|..||.|.|.:.||.||.||:|.|.|.
Zfish   648 SQLDSDNDGIGDDCDEDDDNDGIPDNHAINGIGPDNCRLISNPNQKDSDSNGVGDVCENDFDNDS 712

  Fly   808 VPNGMDNCPNNSMIHHTDFRTLQTIPLDPKGLSQADPNWVVHANGTEIVQTLNSDPGLAVGKDAF 872
            |.:.:|.||.::.:..||||..||:.|||:|.:|.||||||...|.|||||:|||||||||..||
Zfish   713 VMDLVDVCPESAEVTLTDFRAYQTVILDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAF 777

  Fly   873 GGVDFDGTFYINDDTDDDYAGFVFSYQSSYKYYVVQWKKGTQTYWEPRPFTASAAPGIQIKLVNS 937
            .||||:|||::|..||||||||:|.||.|..:|||.||:..||||:..||.|.|.||:|:|.|.|
Zfish   778 NGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQTEQTYWQSIPFRAMAEPGLQLKAVKS 842

  Fly   938 TEGPGPMMRNSLWHEGNTDGEARLLWKDPKNIAWKERTSYRWSLVHRPAIGLIRLQMHEGNRLIF 1002
            ..|||..:||:|||.|:||||.:||||||:|:.|.::|||||.|.|||.:|.||::::||:.::.
Zfish   843 RTGPGEFLRNALWHAGDTDGEVKLLWKDPRNVGWLDKTSYRWQLSHRPQVGYIRVKLYEGSEMVA 907

  Fly  1003 DSGNVFDSTLKGGRLGVFCFSQRMIIWSNLQYKCNNRVKPLIYNDLSDYLK 1053
            ||..|.|::::|||||||||||..||||||:|:||:.|.    .|.|.:.|
Zfish   908 DSDVVIDTSMRGGRLGVFCFSQENIIWSNLRYRCNDTVP----EDFSSHRK 954

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tsp	NP_001137802.1	EGF_CA	477..513	CDD:284955	19/36 (53%)
		TSP3 repeat_1C	563..595	CDD:275367	15/33 (45%)
		TSP3 repeat_short	632..654	CDD:275365	10/21 (48%)
		TSP_3	656..690	CDD:280556	22/33 (67%)
		TSP3 repeat_long	656..690	CDD:275366	22/33 (67%)
		TSP3 repeat_short	691..713	CDD:275366	14/21 (67%)
		TSP3 repeat_long	714..751	CDD:275365	17/36 (47%)
		TSP_3	715..751	CDD:280556	17/35 (49%)
		TSP3 repeat_short	752..787	CDD:275366	21/41 (51%)
		TSP_3	789..822	CDD:280556	14/32 (44%)
		TSP_C	841..1038	CDD:283408	121/196 (62%)
		coiled coil	245..286	CDD:293927	11/40 (28%)
		TSPcc_insect	245..286	CDD:293927	11/40 (28%)
		EGF_CA	343..377	CDD:214542	4/33 (12%)
		EGF_CA	421..457	CDD:238011	14/35 (40%)
thbs3a	NP_775332.1	LamG	<58..196	CDD:304605	27/195 (14%)
		coiled coil	230..272	CDD:293925	11/41 (27%)
		TSP-3cc	230..272	CDD:293925	11/41 (27%)
		EGF_CA	319..354	CDD:238011	13/34 (38%)
		EGF_CA	373..408	CDD:238011	18/34 (53%)
		TSP type-3 1	459..493		15/33 (45%)
		TSP type-3 2	494..529		21/34 (62%)
		TSP3 repeat_long	494..529	CDD:275366	21/34 (62%)
		TSP_3	495..529	CDD:280556	20/33 (61%)
		TSP type-3 3	530..552		10/21 (48%)
		TSP3 repeat_short	530..552	CDD:275366	10/21 (48%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	548..704		85/155 (55%)
		TSP3 repeat_long	553..588	CDD:275366	22/34 (65%)
		TSP_3	554..588	CDD:280556	22/33 (67%)
		TSP3 repeat_short	589..611	CDD:275365	14/21 (67%)
		TSP3 repeat_long	612..649	CDD:275365	17/36 (47%)
		TSP_3	613..649	CDD:280556	17/35 (49%)
		TSP3 repeat_short	650..692	CDD:275366	21/41 (51%)
		TSP type-3 8	693..728		14/34 (41%)
		TSP_3	694..727	CDD:280556	14/32 (44%)
		TSP_C	746..943	CDD:283408	121/196 (62%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170592970
Domainoid	1	1.000	285	1.000	Domainoid score	I1574
eggNOG	1	0.900	-	-		E1_2CMRF
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	738	1.000	Inparanoid score	I580
OMA	1	1.010	-	-		QHG49685
OrthoDB	1	1.010	-	-		D120983at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm26215
orthoMCL	1	0.900	-	-		OOG6_101187
Panther	1	1.100	-	-	O	PTHR10199
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X995
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	13	12.770