DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tsp and Thbs3

DIOPT Version :9

Sequence 1:	NP_001137802.1	Gene:	Tsp / 33941	FlyBaseID:	FBgn0031850	Length:	1060	Species:	Drosophila melanogaster
Sequence 2:	NP_038719.2	Gene:	Thbs3 / 21827	MGIID:	98739	Length:	956	Species:	Mus musculus

Alignment Length:	1076	Identity:	419/1076 - (38%)
Similarity:	556/1076 - (51%)	Gaps:	211/1076 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    16 LTFVDVASL--SLDPVASAE--LEQFIRKGDV-VISTRHIRPR---------------RKLHISI 60
            |..:|:.::  |...||.||  ....:..||: ::||..:.|:               |.|..|:
Mouse    25 LQVIDLLTVGESRQMVAVAEKIRTALLTAGDIYLLSTFRLPPKQGGVLFGLYSRQDNTRWLEASV 89

  Fly    61 EALFMIDFPMLKHKMSFFLDRKQQRVTLDISANGATESRNFEIPNINETSTIRSLALQFSKNR-- 123
            ..           |::..|.|.|:.       :|...:.|.:...:.:..|..:|......:|
Mouse    90 VG-----------KINKVLVRYQRE-------DGKVHAVNLQQAGLADGRTHTALLRLRGPSRPS 136

  Fly   124 --ITLHVDCKASTHHDIDMNLAKLYTQMDDPVIKLFRERKYPLHFDGDMEHSLQRANCQKGNHRR 186
              :.|:||||....|      |.|......|          |....| :|            .|.
Mouse   137 PGLQLYVDCKLGDQH------AGLPALAPIP----------PAEVSG-LE------------IRT 172

  Fly   187 GNRRMLRNKITEREKNKKRDVRGWYEP-------TIAREGVVDHRHQEVPTDVERGDIPVLN--- 241
            |.:..||             ::|:.|.       ::||.|.:    .|.|.   :||..:.|
Mouse   173 GQKAYLR-------------MQGFVESMKIILGGSMARVGAL----SECPF---QGDDSIHNAVT 217

  Fly   242 -------GDCEDALARSLSDLLALVKLLREDVAHQRQEIAYLRMLLENCAGCKNPLTTDNQLRIE 299
                   |:...||...|:....::..||:|:..|.:|::.:|..:..|..|
Mouse   218 SALQSILGEQTKALVTQLTLFNQILVELRDDIRDQVKEMSLIRNTIMECQVC------------- 269

  Fly   300 PDCRSANPCYPGVECLDSAAGPRCGHCPLGFIGDGKSCKPGVTCAHHMCYPGVQCHDTVNGAQCD 364
                                         ||                        |:.
Mouse   270 -----------------------------GF------------------------HEQ------- 274

  Fly   365 SCPAGYEGDGRTCSLRNPCLDTPCPSGAQCLQVGYPPYFHCISCPMGHEVNGTSCRDMNECLLYD 429
                           |:.|..:||..|..|::|...|.:.|..||.|.:.|||.|.|:|||...|
Mouse   275 ---------------RSHCSPSPCFRGVDCMEVYEYPGYRCGPCPPGLQGNGTHCDDINECAHAD 324

  Fly   430 PCDELATCTNLSPGFQCSPCPVGFDGTHAHGYFADYYSVDYRRQTCLDVDECRTG-FFRCPEHST 493
            ||...::|.|..|||.|..||.|:.||...|...||....  :|.|.|:|||..| ...|..:|.
Mouse   325 PCFPGSSCINTMPGFHCEACPPGYKGTRVSGVGIDYARAS--KQVCNDIDECNDGNNGGCDPNSI 387

  Fly   494 CINEIGSYRC-QCHEGYVTNGTYSCLDRSSVFMC--PDGTVCDRNAVCLRMDNIRHKCHCNVGWA 555
            |.|.:||::| .|..|::.|.:..|:...:   |  |..:.|..:|.||...|....|.||||||
Mouse   388 CTNTVGSFKCGPCRLGFLGNQSQGCVPART---CHSPAHSPCHIHAHCLFERNGAVSCQCNVGWA 449

  Fly   556 GNGLICGRDTDVDGWPDQAIGCPE--LRCQRDNCPKLPNSGQEDADLDGHGDGCDDDADGDNVQN 618
            |||.:||.|||:||:||||:.|.:  ..|::|||...|||||||||.||.||.||||||||.::|
Mouse   450 GNGNVCGPDTDIDGYPDQALPCMDNNKHCKQDNCLLTPNSGQEDADNDGVGDQCDDDADGDGIKN 514

  Fly   619 SQDNCWLAYNTEQLDSDGDKVGDVCDNCVLKYNPRQLDTDEDGLGDECDGDIDNDSIPNALDNCP 683
            .:|||.|..|.:|.:||.|..||.||||....|..|.|||.:|.||.||.|:|.|.|||.|||||
Mouse   515 VEDNCRLFPNKDQQNSDTDSFGDACDNCPNVPNNDQKDTDGNGEGDACDNDVDGDGIPNGLDNCP 579

  Fly   684 LLPNPSQSDVDNDGVGDACDNCPNLPNPDQKDRDMDFVGDACHRDIDGDDDGVPNSLDNCPMVSN 748
            .:|||.|:|.|.||||||||:||.:.||.|.|.|.|.|||.|..:.|.|.||..::.||||.:.|
Mouse   580 KVPNPLQTDRDEDGVGDACDSCPEMSNPTQTDADSDLVGDVCDTNEDSDGDGHQDTKDNCPQLPN 644

  Fly   749 SDQLDTDGDGTGDECDDDMDGDGIPNY----KDNCPLAKNPKQDDFNRNGKGDSCEDDEDVDGVP 809
            |.|||:|.||.|||||.|.|.||:|:|    .|||.|..||.|.|.:.||.||.||||.|.|.|.
Mouse   645 SSQLDSDNDGLGDECDGDDDNDGVPDYIPPGPDNCRLVPNPNQKDSDGNGVGDVCEDDFDNDAVV 709

  Fly   810 NGMDNCPNNSMIHHTDFRTLQTIPLDPKGLSQADPNWVVHANGTEIVQTLNSDPGLAVGKDAFGG 874
            :.:|.||.::.:..||||..||:.|||:|.:|.||||||...|.|||||:|||||||||..||.|
Mouse   710 DPLDVCPESAEVTLTDFRAYQTVILDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNG 774

  Fly   875 VDFDGTFYINDDTDDDYAGFVFSYQSSYKYYVVQWKKGTQTYWEPRPFTASAAPGIQIKLVNSTE 939
            |||:|||::|..||||||||:||||.|.::|||.||:..||||:..||.|.|.||:|:|.|.|..
Mouse   775 VDFEGTFHVNTVTDDDYAGFLFSYQDSGRFYVVMWKQTEQTYWQATPFRAVAQPGLQLKAVTSIS 839

  Fly   940 GPGPMMRNSLWHEGNTDGEARLLWKDPKNIAWKERTSYRWSLVHRPAIGLIRLQMHEGNRLIFDS 1004
            |||..:||:|||.|:|..:.||||.||:|:.|:::|||||.|:|||.:|.||::::||.:|:.||
Mouse   840 GPGEHLRNALWHTGHTPDQVRLLWTDPRNVGWRDKTSYRWRLLHRPQVGYIRVKLYEGPQLVADS 904

  Fly  1005 GNVFDSTLKGGRLGVFCFSQRMIIWSNLQYKCNNRV 1040
            |.:.|::::|||||||||||..||||||||:||:.|
Mouse   905 GVIIDTSMRGGRLGVFCFSQENIIWSNLQYRCNDTV 940

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tsp	NP_001137802.1	EGF_CA	477..513	CDD:284955	14/37 (38%)
		TSP3 repeat_1C	563..595	CDD:275367	17/33 (52%)
		TSP3 repeat_short	632..654	CDD:275365	10/21 (48%)
		TSP_3	656..690	CDD:280556	22/33 (67%)
		TSP3 repeat_long	656..690	CDD:275366	22/33 (67%)
		TSP3 repeat_short	691..713	CDD:275366	14/21 (67%)
		TSP3 repeat_long	714..751	CDD:275365	17/36 (47%)
		TSP_3	715..751	CDD:280556	17/35 (49%)
		TSP3 repeat_short	752..787	CDD:275366	22/38 (58%)
		TSP_3	789..822	CDD:280556	15/32 (47%)
		TSP_C	841..1038	CDD:283408	121/196 (62%)
		coiled coil	245..286	CDD:293927	10/40 (25%)
		TSPcc_insect	245..286	CDD:293927	10/40 (25%)
		EGF_CA	343..377	CDD:214542	1/33 (3%)
		EGF_CA	421..457	CDD:238011	17/35 (49%)
Thbs3	NP_038719.2	LamG	21..193	CDD:304605	42/227 (19%)
		coiled coil	227..269	CDD:293925	10/41 (24%)
		TSP-3cc	227..269	CDD:293925	10/41 (24%)
		EGF_CA	316..352	CDD:238011	17/35 (49%)
		EGF_3	378..407	CDD:289699	9/28 (32%)
		TSP type-3 1	457..491		17/33 (52%)
		TSP3 repeat_1C	457..491	CDD:275367	17/33 (52%)
		TSP type-3 2	492..527		22/34 (65%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	518..537		8/18 (44%)
		TSP type-3 3	528..550		10/21 (48%)
		TSP3 repeat_short	528..550	CDD:275365	10/21 (48%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	546..699		86/152 (57%)
		TSP3 repeat_long	551..586	CDD:275366	22/34 (65%)
		TSP_3	552..586	CDD:280556	22/33 (67%)
		TSP3 repeat_short	587..609	CDD:275366	14/21 (67%)
		TSP3 repeat_long	610..647	CDD:275365	17/36 (47%)
		TSP_3	611..647	CDD:280556	17/35 (49%)
		TSP type-3 8	688..723		15/34 (44%)
		TSP_3	689..722	CDD:280556	15/32 (47%)
		TSP_C	741..938	CDD:283408	121/196 (62%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167847612
Domainoid	1	1.000	283	1.000	Domainoid score	I1633
eggNOG	1	0.900	-	-		E1_2CMRF
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	764	1.000	Inparanoid score	I542
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG49685
OrthoDB	1	1.010	-	-		D120983at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm43939
orthoMCL	1	0.900	-	-		OOG6_101187
Panther	1	1.100	-	-	O	PTHR10199
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4962
SonicParanoid	1	1.000	-	-		X995
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.800