DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tsp and thbs2a

DIOPT Version :9

Sequence 1:	NP_001137802.1	Gene:	Tsp / 33941	FlyBaseID:	FBgn0031850	Length:	1060	Species:	Drosophila melanogaster
Sequence 2:	XP_009305765.1	Gene:	thbs2a / 100535241	ZFINID:	ZDB-GENE-020708-2	Length:	1175	Species:	Danio rerio

Alignment Length:	1140	Identity:	392/1140 - (34%)
Similarity:	535/1140 - (46%)	Gaps:	280/1140 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    87 TLDI--SANGATESRNFEIPNINETSTIRSLALQFSKNRITLHVDCKASTHHDIDMNLAKLYTQM 149
            |||:  ...|:....:||..:::: |..::::|........|:|.|..     ||..:      :
Zfish   127 TLDLVYMVKGSQNVVSFEDVDLSD-SQWKNISLYVHGENAHLYVGCSL-----IDSVI------L 179

  Fly   150 DDPVIKLFRE---------------------RKYPLHFDGDMEHSLQRANCQKGNHRRGN----- 188
            |:|..:|.|.                     :.....||..:|:.|:...|:.......|
Zfish   180 DEPFYELLRPEGSQMFVAKGSIRENHFRGLLQNVQFLFDTPIENILRNKGCEIAKPEEVNVVNES 244

  Fly   189 -------RRMLRNKITEREKNKKRDVRGWYEPTIAREGVVDHRHQEVPTDV-ERGDIPVLNGDCE 245
                   ..:..|.|.|:||             ||.: |.:...:|:...| |...:.::.|:..
Zfish   245 TESVSVGTSISTNFIGEKEK-------------IASD-VCERSCEELSNMVQELKGLRIIVGNLI 295

  Fly   246 DALARSLSDLLALVKLLREDVAHQRQEIAYLRM--------------LLENCAGCK--------- 287
            |.|.:...:     ..:.::|....:.|...||              ::::|..|.
Zfish   296 DGLQKVTEE-----NTVMKEVLGNMKNIKDKRMCWQDGRLFEDKEDWVVDSCTKCTCQESKIVCH 355

  Fly   288 ----NPLTTDNQLRIEPDC--------------------------------------RSANPCY- 309
                .|:...:...::.:|                                      .::|||.
Zfish   356 QITCPPVACASPTFLDGECCPLCLPKDSEDGWSPWSEWTECTVTCGTGTQQRGRSCDATSNPCSG 420

  Fly   310 PGVECLDSAAGP----------------------RCG--------HC--PLGFIGDGKSC----- 337
            |.::....:.|.                      .||        ||  |:..:| ||.|
Zfish   421 PSIQTRRCSLGKCDSRVRQDGGWSLWSPWSSCSVTCGEGQITRIRHCNSPVPQLG-GKDCEGSGR 484

  Fly   338 -------KP----------------GVTCA------HHMC------YPGVQC-HDTVNGAQCD-- 364
                   ||                .|||.      ..:|      |.|.:| .|:|:...|:
Zfish   485 ETQKCEAKPCPIDGAWGPWSPWAVCSVTCGGGTRTRTRVCNSPRPQYGGKKCPGDSVDTNACNKQ 549

  Fly   365 SCPAGYEGDGRTCSLRNPCLDTPCPSGAQCLQVGYPP-YFHCISCPMGHEVNGTSCRDMNEC-LL 427
            .||.    ||        ||..||..|.:|  ...|. .:.|..||:|...||..|.|:||| ::
Zfish   550 DCPV----DG--------CLSNPCFGGVEC--TSSPDGSWECGPCPLGFRGNGKVCEDVNECDMV 600

  Fly   428 YDPCDELA---TCTNLSPGFQCSPCPVGFDGTHAHGYFADYYSVDYRRQTCLDVDECRTGFFRCP 489
            .|.|.::.   .|.|..|||.|.|||..:.|....|...:  :....:|.|...:.|:.
Zfish   601 PDLCYKVGDSQRCVNTDPGFHCLPCPPRYKGNQPFGMGVE--AAKLNKQVCEQENPCKE------ 657

  Fly   490 EHSTCINEIGSYRCQCHEGYVTNGTYSCLDRSSVFMCPDGTVCDRNAVCLRMDNIRH---KCHCN 551
                          :.|.                        |.|:|.|:.:.:...   ||.|.
Zfish   658 --------------KTHN------------------------CHRSAECIYLGHFSEPMFKCECK 684

  Fly   552 VGWAGNGLICGRDTDVDGWPDQAIGC---PELRCQRDNCPKLPNSGQEDADLDGHGDGCDDDADG 613
            :|:||:|||||.|:|:||||:|.:.|   ....|::|||||||||||||.|.||.||.||.|.|.
Zfish   685 IGYAGDGLICGEDSDLDGWPNQNLVCGANQSYHCKKDNCPKLPNSGQEDFDKDGQGDACDKDDDN 749

  Fly   614 DNVQNSQDNCWLAYNTEQLDSDGDKVGDVCDNCVLKYNPRQLDTDEDGLGDECDGDIDNDSIPNA 678
            |.:.:..|||.|.||..|.|.|.|.|||.||||..::||.|:|||.:|.||.|..|||.|.:.|.
Zfish   750 DGIMDETDNCPLLYNPRQFDYDKDLVGDRCDNCPYEHNPAQIDTDGNGEGDACAVDIDGDEVLNE 814

  Fly   679 LDNCPLLPNPSQSDVDNDGVGDACDNCPNLPNPDQKDRDMDFVGDACHRDIDGDDDGVPNSLDNC 743
            .||||.:.|..|.|.|.|||||.|||||.|.||:|.|.|.|.|||.|..:.|.|:||..||||||
Zfish   815 NDNCPYMYNTDQKDTDMDGVGDQCDNCPLLHNPEQTDSDNDRVGDECDNNQDIDEDGHQNSLDNC 879

  Fly   744 PMVSNSDQLDTDGDGTGDECDDDMDGDGIPNYKDNCPLAKNPKQDDFNRNGKGDSCEDDEDVDGV 808
            |.:.|::|.|.|.||.||.||.|.|.||:|:.||||.|..|..|.|.:.:|:||:|:||.|.|.:
Zfish   880 PYIPNANQADHDKDGKGDACDSDDDNDGVPDDKDNCRLVPNKDQRDSDGDGRGDACKDDFDNDSI 944

  Fly   809 PNGMDNCPNNSMIHHTDFRTLQTIPLDPKGLSQADPNWVVHANGTEIVQTLNSDPGLAVGKDAFG 873
            |:.||.||.|..|.:||||..|.:.|||||.:|.||||||...|.|:|||.|||||:|||.|.|.
Zfish   945 PDFMDVCPENDAISNTDFRKFQMVHLDPKGTAQIDPNWVVRHQGKELVQTANSDPGIAVGFDEFN 1009

  Fly   874 GVDFDGTFYINDDTDDDYAGFVFSYQSSYKYYVVQWKKGTQTYWEPRPFTASAAPGIQIKLVNST 938
            .|||.||||:|.|.|||||||||.||||.::|||.||:.||||||..|..|....|:.:|:||||
Zfish  1010 AVDFSGTFYVNTDRDDDYAGFVFGYQSSGRFYVVMWKQITQTYWEDNPSKAIGISGVSLKVVNST 1074

  Fly   939 EGPGPMMRNSLWHEGNTDGEARLLWKDPKNIAWKERTSYRWSLVHRPAIGLIRLQMHEGNRLIFD 1003
            .|.|..:||:|||.|:|.|:.|.||.|||||.||:.|:|||.|:|||..|.||:.::||.:::.|
Zfish  1075 TGTGEHLRNALWHTGDTPGQVRTLWHDPKNIGWKDYTAYRWHLIHRPKTGFIRVVVYEGKQIMAD 1139

  Fly  1004 SGNVFDSTLKGGRLGVFCFSQRMIIWSNLQYKCNN 1038
            ||.|:|.|..|||||:|.|||.::.:|:|:|:|.:
Zfish  1140 SGPVYDQTFAGGRLGLFVFSQELVYFSDLKYECRD 1174

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tsp	NP_001137802.1	EGF_CA	477..513	CDD:284955	2/35 (6%)
		TSP3 repeat_1C	563..595	CDD:275367	18/34 (53%)
		TSP3 repeat_short	632..654	CDD:275365	12/21 (57%)
		TSP_3	656..690	CDD:280556	17/33 (52%)
		TSP3 repeat_long	656..690	CDD:275366	17/33 (52%)
		TSP3 repeat_short	691..713	CDD:275366	15/21 (71%)
		TSP3 repeat_long	714..751	CDD:275365	19/36 (53%)
		TSP_3	715..751	CDD:280556	19/35 (54%)
		TSP3 repeat_short	752..787	CDD:275366	19/34 (56%)
		TSP_3	789..822	CDD:280556	15/32 (47%)
		TSP_C	841..1038	CDD:283408	115/196 (59%)
		coiled coil	245..286	CDD:293927	7/54 (13%)
		TSPcc_insect	245..286	CDD:293927	7/54 (13%)
		EGF_CA	343..377	CDD:214542	12/48 (25%)
		EGF_CA	421..457	CDD:238011	16/39 (41%)
thbs2a	XP_009305765.1	TSPN	30..219	CDD:214560	18/103 (17%)
		VWC	324..378	CDD:278520	4/53 (8%)
		TSP_1	388..433	CDD:278517	5/44 (11%)
		TSP1	443..495	CDD:214559	11/52 (21%)
		TSP1	500..552	CDD:214559	10/51 (20%)
		EGF_3	655..694	CDD:289699	14/82 (17%)
		TSP3 repeat_1C	696..731	CDD:275367	18/34 (53%)
		TSP3 repeat_long	732..767	CDD:275366	18/34 (53%)
		TSP3 repeat_short	769..790	CDD:275365	12/20 (60%)
		TSP_3	792..826	CDD:280556	17/33 (52%)
		TSP3 repeat_long	792..826	CDD:275366	17/33 (52%)
		TSP3 repeat_short	827..849	CDD:275366	15/21 (71%)
		TSP3 repeat_long	850..887	CDD:275365	19/36 (53%)
		TSP_3	851..887	CDD:280556	19/35 (54%)
		TSP3 repeat_short	888..923	CDD:275366	19/34 (56%)
		TSP_3	925..954	CDD:280556	14/28 (50%)
		TSP_C	977..1174	CDD:283408	115/196 (59%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170592963
Domainoid	1	1.000	70	1.000	Domainoid score	I9497
eggNOG	1	0.900	-	-		E1_2CMRF
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D120983at2759
OrthoFinder	1	1.000	-	-		FOG0001167
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101187
Panther	1	1.100	-	-	O	PTHR10199
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	8	7.750