Protein Alignment PDZ-GEF and rapgef2a

DIOPT Version :10

• Sequence 1: NP_609012.2
• Gene: PDZ-GEF / 33881
• FlyBaseID: FBgn0265778
• Length: 1573
• Species: Drosophila melanogaster
• Sequence 2: XP_005170987.3
• Gene: rapgef2a / 793952
• ZFINID: ZDB-GENE-090313-280
• Length: 1486
• Species: Danio rerio
• Alignment Length: 1582
• Identity: 560/1582 - (35%)
• Similarity: 794/1582 - (50%)
• Gaps: 355/1582 - (22%)

  Fly    29 INRPEL----HQKCNRGS--------------HSSDTSS-------AYSGSDTMASNY-ASSLEA 67
            :::|.|    |.:|.:..              ||..|||       :.|||.:::..| |:..|.
Zfish   169 VSKPPLPPGYHSECPKSQLPTDFSKLHPSDSCHSHMTSSQSRSSIASDSGSSSLSDIYQATESEC 233

  Fly    68 EEIDLSGLVESVVDSDE----EDLAESMDSLTVRDAVRDCLEKDPAERSEEDVEVLLEFTQGLKA 128
            .::|||||.|:.|||||    ||:....|.|..||.|||||||||.:|:::|:|.||||.|.|.|
Zfish   234 GDMDLSGLPETAVDSDEDDDDEDVGRGTDHLMSRDIVRDCLEKDPMDRTDDDIEQLLEFVQQLPA 298

  Fly   129 FTNITLAVRRALCSVMVFAVVDKAGTVVMSDGEELDSWSVLINGAVEIEHANGSREELQMGDSFG 193
            |.:::::|||.||:|||||||::|||:|::.|||||||||::|||||:.:.:|..|.:.||.|||
Zfish   299 FASLSVSVRRELCAVMVFAVVERAGTIVLNHGEELDSWSVILNGAVEVIYLDGRSESVCMGGSFG 363

  Fly   194 ILPTMDKLYHRGVMRTKCDDCQFVCITQTDYYRIQHQGEENTRRHEDENGFVVMVTELRSIGGAG 258
            :.|:|:|....||||||.||||||||.|.||..|.:|.|:||:|.|:| |.:|||.|.|.:...|
Zfish   364 VCPSMEKQLMTGVMRTKVDDCQFVCIAQQDYCCILNQVEQNTQRVEEE-GEIVMVKEHRELDRTG 427

  Fly   259 TDSAGSGGSATGASASLNMKRGHVVIRGTPERLLQQLVEENSMTDPTYVEDFLLTHRIFIQNPQE 323
            |                  ::||:|::||.|||:|.|||::|:.||||:||||||:|.|:.:|..
Zfish   428 T------------------RKGHIVVKGTAERLMQHLVEDHSIVDPTYIEDFLLTYRTFLPSPLI 474

  Fly   324 VTSKLLHWFDLEQVDAHKTQELRDRVTRVVLLWVNNHFTDFEADYEMMEFLEVFEALLERKKLLS 388
            :..:||.||:        ...|||:|||||||||||||:|||.|.:|..|||.|::.|||:|:..
Zfish   475 LGQRLLDWFN--------DPCLRDKVTRVVLLWVNNHFSDFEGDPDMTSFLEEFQSNLEREKMTG 531

  Fly   389 QLRLLHIACAAKARMRSCTLTRSSRDEPLNFRIVGGYELRGVAIATGNAAVGIYISHVEPGSKAQ 453
            |||||:|||||||:.|..|:||::|:.||.|.::||.| ||..         ::||.||.||||:
Zfish   532 QLRLLNIACAAKAKPRVITVTRTAREIPLPFTLIGGAE-RGTR---------LFISSVEVGSKAE 586

  Fly   454 DVGLKRGDQIHEVNGQSLDHVTSKRALEILTGTTHLSISVKSNLLGFKEIMQ--ALEHGGGTAGS 516
            :.||||||||.|||||:.::|...:|.|||.....||:|||:|||.|||::.  |.|...|....
Zfish   587 EAGLKRGDQILEVNGQTFENVQLSKATEILRNNIQLSMSVKTNLLVFKELVARVAEERKNGVPHL 651

  Fly   517 GSISAGSGSFKSVRSPRRICANDIAKLHGRSDSTTDELSSVSASNRAHMVRLSSVDMLLDQPDCA 581
            ..|..|          ::.|...|.:|...||....|  .:|...:||.|
Zfish   652 PKIGDG----------KKNCRYSIPELGAGSDVIGQE--KISKKAKAHTV--------------- 689

  Fly   582 PPQTPPVSGSGNMASNFMQQLLQSVNNSSAKKSGGNSNSDQQDTKGGFMTLAPKRRL----QKAL 642
                                             ||.:.         .|.:..|.|:    ||..
Zfish   690 ---------------------------------GGRNK---------LMKILDKTRMSILPQKPY 712

  Fly   643 AKMNLLNKQNHGSSLNDSSDTLLNDPKSKLSAVSSCSSSTQSSINGCTVSGGGRLYQSQSNPDLT 707
            :.:.|...|:         |:::...::|.:..:...|.|.||                |||||.
Zfish   713 SDLGLGQTQD---------DSIVGLRQNKQTPPTMPVSGTLSS----------------SNPDLA 752

  Fly   708 SLNYDGGSDAGGNGGGRLQVNYLNAHIHRPSAASTLTTNSTQSHLLPDYP------DHVLKVYKA 766
            .             |.:..::|                 |||    |..|      |.||:::||
Zfish   753 Q-------------GQQRIIDY-----------------STQ----PPAPGFTQLQDQVLRIFKA 783

  Fly   767 DQTCKYVLIYKETTAHEVVMLTLQEFGIHDPSSNFSLCEVSVGDGGMVKQRRLPDQLQNLAERIS 831
            ||..:|:|..::|||.|...|.::|||:......||||||||...|::|||||||||..||:||.
Zfish   784 DQQSRYLLANRDTTAREAANLAIKEFGLSASPEAFSLCEVSVTQEGVIKQRRLPDQLSKLADRIQ 848

  Fly   832 FAARYYLKLNDSTEPLVPDELALELVRESNVHFLHLNAYELAIQLTLQDFANFRQIESTEYVDEL 896
            .:||||||.|..||.|..|..|||:.|||.|..|.|::.|:|.||:.:::..|..||.|:|:::|
Zfish   849 LSARYYLKSNMETETLCSDVEALEMQRESVVPLLSLSSMEIANQLSARNYLLFSCIEPTDYINDL 913

  Fly   897 FELRSRYGVPMLSKFAELVNREMFWVVSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNSMFAI 961
            |:|..:.....|..|..|||:|.|||.:||..|.|:.:|:||:|.|||||.||::|:||||||||
Zfish   914 FKLHPQEPPTNLRNFEGLVNQETFWVATEIVQETNLAKRVKIIKHFIKIALHCRDCKNFNSMFAI 978

  Fly   962 VSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYPIVK 1026
            :||.....|||||.|||:||.||::|..:|||:.||||||:|||.|::...| |.|:||.:|::|
Zfish   979 ISGFNLAPVSRLRSTWERLPGKYEKLLAELQDVFDPSRNMAKYRNLLNKHNL-QPPVIPLFPVIK 1042

  Fly  1027 KDLTFIHLGNDTRVDGLINFEKLRMLAKEVRLLTHMCSSPYDLLSILELKGQSPSNALFSLNQMS 1091
            |||||:|.||::.||||:|||||||:|:|:|.:..|.|...|...:.:.: :....:|.||:|:|
Zfish  1043 KDLTFLHEGNNSEVDGLVNFEKLRMIAREIRHVVRMASITMDPALLFKTR-KKKWRSLGSLSQVS 1106

  Fly  1092 ASQSNAAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDEDLLHKF 1156
            :|.         .::.|....||:.:.::..:.||::|...|.|||:.||::.....:||.||:.
Zfish  1107 SSS---------LSDIGTMGGKRKGRRSSFLSAKKLYEVEMMSRRVRQYLDTHSNECNEDTLHEL 1162

  Fly  1157 SLECEPAHGSTYSGSISHGNTSHR--SGGGGSISGGAGGSSGGGGGGSSSLNAGDQLSIYSHTSS 1219
            |:.||||:.||    :.|.....|  :...|..|.....::|         |.|.:.||     |
Zfish  1163 SMTCEPANSST----LKHAGERRRIDASPAGQRSASPQQTNG---------NHGRKKSI-----S 1209

  Fly  1220 SSAPNSSLSLRKRHPSSPTLSTTSSTSSTSDHQRRQMHNNGPKFGTASPQAVKKMLSLSESSKIR 1284
            ...|......:::.|.. :.|:|||..|:..:..:.....||:...:.......:||.|.|..:.
Zfish  1210 KELPFEGRDRQRKLPED-SQSSTSSLLSSPRNSPQSTPRKGPQLVVSEVSDQMSLLSSSSSELLM 1273

  Fly  1285 PHQPFVPRHGSTMAGVIPPLHHMHAAHGFSTPSPGGVVTSPATSAVANVQCTPSPSPCSHRRLAS 1349
            ...                  |.|              |.|.:.|          .|.|.||
Zfish  1274 VDD------------------HTH--------------TQPHSLA----------HPLSARR--- 1293

  Fly  1350 GGNIIPSRAIHERSHSDTPAPPPPLPSVDLSLESSSVTTFRDLPLRKSVTS-----GSISSCDSG 1409
                               |.|.     .|||.|.|:|  :|...|.|:.:     ||.:||.||
Zfish  1294 -------------------AEPD-----QLSLGSYSLT--QDQCDRASLDAADSGRGSWTSCSSG 1332

  Fly  1410 YVHQQQHYHLQ--------YQQQQQQNSS-----QHEPSPPVYTAADCRLLQQISNNAVTRNLNS 1461
                 .|.::|        |..:..:..|     .|..:|..|.|.:..     .:....:....
Zfish  1333 -----SHDNIQSIPQRVGYYDSRSWEMLSDGMGRSHMTTPTGYWADELE-----GDTGTIKRRGG 1387

  Fly  1462 PCQSTNTPPSTPT--------PPPNQPTATIQLSAPPTAAAYMHAR--SQHQQLQQQQQSLAMPP 1516
            ..::.||..:|.:        |||..|..|....|.....::.|.|  ..:....|:.:.|....
Zfish  1388 KDETPNTNKNTVSRREGHFREPPPTPPGYTAVSLAEADTNSHAHGRRPPDYSAALQRSRFLKRTC 1452

  Fly  1517 PPPPPYN---VPPLGSIYSHHQ 1535
            .|||.::   :||    |||.|
Zfish  1453 DPPPLHSSSRLPP----YSHCQ 1470

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999	64/108 (59%)
		RasGEFN	279..400	CDD:214571	68/120 (57%)
		PDZ_RapGEF2_RapGEF6-like	404..496	CDD:467237	44/91 (48%)
		RA_PDZ-GEF1	759..841	CDD:340483	44/81 (54%)
		RasGEF	871..1050	CDD:459872	96/178 (54%)
rapgef2a	XP_005170987.3	None