DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment PDZ-GEF and rasgrp4

DIOPT Version :9

Sequence 1:	NP_609012.2	Gene:	PDZ-GEF / 33881	FlyBaseID:	FBgn0265778	Length:	1573	Species:	Drosophila melanogaster
Sequence 2:	XP_005173714.1	Gene:	rasgrp4 / 558453	ZFINID:	ZDB-GENE-050419-110	Length:	725	Species:	Danio rerio

Alignment Length:	710	Identity:	143/710 - (20%)
Similarity:	236/710 - (33%)	Gaps:	222/710 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly   838 LKLNDSTEPLVPDELALELVRESNVHFLHLNAYELAIQLTLQDFANFRQIESTEYVDELFELRSR 902
            :.::.:..|||..       |:.::.|.|:...|:|..|:..:|.||..:...:|       ||
Zfish   175 VNISQTPSPLVKK-------RKVSLLFDHMEPDEMAEHLSYLEFKNFCNVSFLDY-------RS- 224

  Fly   903 YGV-------PMLSKFAELVNREMFWVVSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNSMFA 960
            |.|       |.|.:...|.|....||...|...|...:|.::..:||.:|:..:..:|||::.|
Zfish   225 YVVHGSVRDNPALERSVMLCNGVSQWVQLMILNRHTSQQRAEVFTKFIHVAQKLRSLQNFNTLMA 289

  Fly   961 IVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYPIV 1025
            ::.||.|.::|||:.|...|.|...:..::|.:|:....|.|.||::.|.....:.||:..:
Zfish   290 VIGGLCHSSISRLKDTASLLSSDVTKTLSELTELLSSYSNYSNYRRVYSECTGFKVPILGVH--- 351

  Fly  1026 KKDLTFIHLGN---DTRVDGLINFEKLRMLAKEVR--LLTHMCSSPY----DLLSILELK----- 1076
            .|||  |.|..   |...|..||..||:.|...:.  |..|.|:.|:    |||.:|.|.
Zfish   352 LKDL--ISLNEALPDYLEDDKINLGKLQHLYSNISDLLAIHDCTPPFEANKDLLHLLTLSLDLYY 414

  Fly  1077 ----------GQSPSNALFSLNQMSASQSNAAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEA 1131
                      .:.|.|...        |..|.....:.|..|.....|...:|.:.:.|:|.:..
Zfish   415 TEDEIYELSYAKEPKNPKI--------QPVAPVKPPVVAEWGSGVTPRLDPATISKHVKQMVDSI 471

  Fly  1132 QMVRRVKAY-LNSLKILSDEDLLHKFSLECEPAHGSTYSGSISHGNTSHRSGGGGSISGGAGGSS 1195
                 :|.| ||....:|.|:.             ...:.:......:|.|...|.||
Zfish   472 -----MKNYDLNMDGYISLEEF-------------EKIAANFPFSFCTHESDREGEIS------- 511

  Fly  1196 GGGGGGSSSLNAGDQLSIYSHTSSSSAPNSSLSLRKRHPSSPTLSTTSSTSSTSDHQRRQMHNNG 1260
                        .::::.|.....|.......:|...|
Zfish   512 ------------REEITSYFMRGMSVCAKLGFNLHNLH--------------------------- 537

  Fly  1261 PKFGTASPQAVKKMLSLSESSKIRPHQPFVPRHGSTMAGVIPPLHHMHAAHGFSTPSPGGVVTSP 1325
                           :..|::..||  .|....|..:.|||...:|....         ||....
Zfish   538 ---------------NFHETTYKRP--TFCDTCGGFLWGVIKQGYHCKDC---------GVNCHK 576

  Fly  1326 ATSAVANVQCTPSPSPCSHRRLASGGNIIPSRAIHERSHSDTPAPPPPLPSVDLSLESSSVTTFR 1390
            ....|..::|.        :||             :.:.|..|..|.|:..  |..:.:|.:.|
Zfish   577 HCKDVVGMECL--------KRL-------------QVNSSSCPCTPGPVSG--LHTKGNSWSEF- 617

  Fly  1391 DLPLRKSVTSGSISSCDSGYVHQQQHYHLQYQQQQQQNSSQHEPSPPVYTAADCRLLQQISNNAV 1455
                .:|..|||.   :..:|.            ...|.|:|...   .|::|        ::|.
Zfish   618 ----SQSFQSGSE---EEAFVF------------PNGNESEHSKG---QTSSD--------SDAE 652

  Fly  1456 TRNLNSPCQSTNTPPSTPTPPPNQPTATIQLSAPPTAAAYMHARSQHQQLQQQQQSLAMP 1515
            ...|:.  :||.|.|...||...:...             :|::.||..|   ::|..:|
Zfish   653 AATLSD--RSTQTEPGVWTPVAKRKDR-------------LHSQKQHPPL---EKSATLP 694

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999
		RasGEFN	279..400	CDD:214571
		PDZ_signaling	404..494	CDD:238492
		DegQ	<422..494	CDD:223343
		PDZ_GEF_RA	757..841	CDD:176380	0/2 (0%)
		RasGEF	871..1050	CDD:279011	55/188 (29%)
		Rhomboid_SP	1252..>1357	CDD:289371	15/104 (14%)
rasgrp4	XP_005173714.1	REM	58..162	CDD:100121
		RasGEF	194..430	CDD:214539	69/248 (28%)
		EF-hand_7	473..519	CDD:290234	11/77 (14%)
		EFh	475..524	CDD:298682	11/80 (14%)
		C1_1	537..586	CDD:278556	13/101 (13%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.910