Protein Alignment PDZ-GEF and sos1

DIOPT Version :10

• Sequence 1: NP_609012.2
• Gene: PDZ-GEF / 33881
• FlyBaseID: FBgn0265778
• Length: 1573
• Species: Drosophila melanogaster
• Sequence 2: NP_001428581.1
• Gene: sos1 / 556286
• ZFINID: ZDB-GENE-070209-128
• Length: 1317
• Species: Danio rerio
• Alignment Length: 1675
• Identity: 303/1675 - (18%)
• Similarity: 520/1675 - (31%)
• Gaps: 594/1675 - (35%)

  Fly    81 DSDEEDLAESMDSLTVRDAVRDCLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMV 145
            |...|:.|.....|.|....:...:..|...|:::.   |::.:||      .|.:...||....
Zfish     9 DFYSEENAHKWRGLLVPSLNKVLNQVHPKLSSQQEA---LQYIEGL------ILVLLNTLCQAQP 64

  Fly   146 FAVVDKAGTVVMSDGEELDSWSVLINGAVEIEHANGSREELQMGDSFGILPTMDKLYHRGVMRTK 210
            ..|.|....|..|....::.|:        |..|..:.|:.:..:... || :||::
Zfish    65 RTVQDVEERVQRSFPHPIEKWA--------IADAQAAIEKRKRRNPLA-LP-VDKIH-------- 111

  Fly   211 CDDCQFVCITQTDYYRIQHQGEENTRRHEDENGFVVMVTELRSIGGAGTDSAGSGGSATGASASL 275
                  ..:.:...|:|.||          .:.::|.|.|..|     .|.....|:........
Zfish   112 ------PLLKEVLGYKIDHQ----------VSVYIVAVLEYIS-----ADILKLAGNYVRNIRHY 155

  Fly   276 NMKRGHVVIRGTPERLLQQL---VEENSMTDPTYVEDFLLTHRIFIQNPQEVTSKLLHWFDLEQV 337
            .:.:..:.:....:::|..:   |||:....|...|:...|..   |:..|:....:
Zfish   156 EISQQDITVAMCADKVLMDMFHQVEEDISVFPLLDEEPSATEE---QSYYELVKTFM-------- 209

  Fly   338 DAHKTQELRD--RVTRVVLLWVNNHFTDFEA-DYEMM--EFLEVFEALLERKKLLSQLRLLHIAC 397
             |...|.|||  .:.||.....|.|...|.| |.|.:  ..::|.|..:   |||..:.
Zfish   210 -AELRQYLRDLNLIIRVFREPFNTHTLLFSAHDVESIFSRIVDVHEVTV---KLLGLIE------ 264

  Fly   398 AAKARMRSCTLTRSSRDEPLNFRIVGGYELRGVAIATGNAAVGIYISHVEPGSKAQDVGLKRGDQ 462
                  .:..:|..|...||     .||....:|   ...|...|.::      |||: |:.|..
Zfish   265 ------DTVEMTDESSPHPL-----VGYCFEDLA---EELAFDPYETY------AQDI-LRSGFH 308

  Fly   463 IHEVN-----GQSLDHVTS-----KRALE------ILTGTTHLSISVKSNLLGFKEIMQALEHGG 511
            .|.::     |.:: ::.|     |.|::      :||...|        .|.:.||::.||...
Zfish   309 EHFLSQLYKPGAAI-YLQSICEGFKEAVQYVLPRLLLTPVYH--------CLHYFEILKQLEEKS 364

  Fly   512 GTAGSGSI--SAGSGSFKSVRSPRRICANDIAK--------------LHGR-------------- 546
            ........  .|.:.......|..|||:..:||              :.|:
Zfish   365 EDEEDKECVKQAITALLNLQSSMERICSKSLAKRRLSESACRFYSQQMKGKHLAIKKMNEIQRNI 429

  Fly   547 ----------------SDSTTDELSSVSASNRAHMVRLSSVDMLLDQPDCAPPQTPPVSGSG-NM 594
                            .:.|   |:.|.|.:..|:.....: |:..:.:...|:.|..|.:. .:
Zfish   430 DGWEGKDIGQCCNEFIMEGT---LTRVGAKHERHIFLFDGL-MICCKSNHGQPRLPGASAAEYRL 490

  Fly   595 ASNFMQQLLQSVNNSSAKK-----------SGGNS---NSDQQDTKGGFM----TLAPKRRLQKA 641
            ...|..:.:| :|:...|:           ..|||   ::...:.|.|:|    :|..:..|::.
Zfish   491 KEKFFMRKVQ-INDKDDKEGEYRHAFEIILKDGNSVVFSAKSAEEKNGWMAALISLQYRSTLERM 554

  Fly   642 LAKMNLLNKQNHGSSLNDSSDTLLNDPKSKLSAVSSCSSSTQSSINGCTVSGGGRLYQSQSNPDL 706
            |....|..::.....|..:......:|.|:.:.|  ...:.||. :|..:...|.:.:
Zfish   555 LDTAMLQEEKEEQMRLPGAQLYRFAEPDSEENVV--FEENVQSK-SGIPIIKAGTVLK------- 609

  Fly   707 TSLNYDGGSDAGGNGGGRLQVNYLNAHIHRPSAASTLTTNSTQSHLLPDYPDHVLKVYKADQTCK 771
                               .:..|..|::..                |::....|..|::  .||
Zfish   610 -------------------LIERLTFHMYAD----------------PNFVRTFLTTYRS--FCK 637

  Fly   772 YVLIYKETTAHEVVMLTLQEFGIHDPSSN---------------------------------FSL 803
                     ..|::.|.::.|.|.:|...                                 .::
Zfish   638 ---------PQELLDLLIERFEIPEPKPTEADQMALENGDQPLSAELKRFRKEFVQPVQLRVLNV 693

  Fly   804 C---------------------EVSVGD--GGMVK----------QRRLPDQLQNLAERISFAAR 835
            |                     |..:|.  |..::          ||:...|....:..|:|.:.
Zfish   694 CRHWVEHHFYDFERDAQLLRRLEEFIGTVRGKAMRKWVESITKIIQRKKQAQASGSSHNITFESS 758

  Fly   836 YYLKLNDSTEPLVPDELALELVRESNVH---FLHLNAYELAIQLTLQDFANFRQIESTEYVDELF 897
                         |..:...|.|...|.   .:.|:..|:|.||||.:...:|.::.:|.|..::
Zfish   759 -------------PPAIEWHLCRAGQVEQFDLMTLHPIEIARQLTLLESDFYRAVQPSELVGSVW 810

  Fly   898 -----ELRSRYGVPMLSKFAELVNREMFWVVSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNS 957
                 |:.|    |.|.:..........|:...|....|:..|:.:|.:.|:|.:..:|..|||.
Zfish   811 TKEDKEINS----PNLLRMIRHTTNLTLWLEKCIVETENVEERVSVVSRIIEILQVFQELNNFNG 871

  Fly   958 MFAIVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDP--SRNMSKYRQLVSAELLAQHPIIP 1020
            :..:||.:....|.||..|:|::||:.:::..:..:|.:.  .:.::|.|.:       ..|.:|
Zfish   872 VLEVVSAMNSSPVYRLDHTFEQIPSRQRKILEEAHELSEDHYKKYLAKLRSI-------NPPCVP 929

  Fly  1021 FYPIVKKDLTFIHLGNDTRV----DGLINFEKLRMLAKEVRLLTHMCSSPYDLLSILELKGQSPS 1081
            |:.|...::.....||...:    ..||||.|.|.:|:....:....:.||.|
Zfish   930 FFGIYLTNILKTEEGNPDFLKRHGTELINFSKRRKVAEITGEIQQYQNQPYCL------------ 982

  Fly  1082 NALFSLNQMSASQSNAAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKAYLNSLKI 1146
                                                 ....:.:|.||.          ||.::.
Zfish   983 -------------------------------------RVENDIRKFFEN----------LNPMED 1000

  Fly  1147 LSDE---DLLHKFSLECEPAHGST-------YSGSISHGNTSHRSGGGGSISGGAGGSS---GGG 1198
            :.::   |.|...|||.||.:..|       ||..:.              |.|...|:   |..
Zfish  1001 MMEKEFTDYLFNKSLEIEPRNARTLPRFAKKYSCPLK--------------SPGVRPSAVRPGAM 1051

  Fly  1199 GGGSSSLNAGDQLSIYS-------HTSSSSAPNSSLSLRKRHPSSPTLSTTSSTSSTSDHQRRQM 1256
            ...:...|...::| ||       .:|::|||||     .|.|.:|..::.:||.:.|...
Zfish  1052 RHPTPLQNEPRKIS-YSRIPDSETESSAASAPNS-----PRTPLTPPPASAASTDTCSVFD---- 1106

  Fly  1257 HNNGPKFGTASPQAVKKMLSLSESSKIRPHQPFVPRHGSTMAGVIPPLHHMHAAHGFSTPSPGGV 1321
                      |||              :|..||........|.|..|       ||
Zfish  1107 ----------SPQ--------------QPSSPFHSNSECVFAPVALP-------HG--------- 1131

  Fly  1322 VTSPATSAVANVQCTPSPSPCSHRRLASGGNIIPSRAIHERSHSDTPAPPPPLPSVDLSLESSSV 1386
               |.:|:|:::.                |.:          .:|.|..|||:|
Zfish  1132 ---PRSSSVSSMM----------------GFL----------RNDDPPVPPPVP----------- 1156

  Fly  1387 TTFRDLPLRKSVTSGSISSCDSGYVHQQQHYHLQYQQQQQQNSSQHEPSPPVYTAADCRLLQQIS 1451
                  |.|:..::.:.||                   ..:..|.|..|||...          .
Zfish  1157 ------PRRRPESAPAESS-------------------PSKMMSTHLDSPPAIP----------P 1186

  Fly  1452 NNAVTRNLNSPCQSTNTPP-STPTPPPNQPTAT--------IQLSAPPTAAAYMHARSQHQQLQQ 1507
            ....:..:.||..|...|| |.|..||.:|..|        :.|..||..     .|:...|...
Zfish  1187 RQPTSSKVYSPRYSLTDPPESPPLLPPREPVRTPDVFCSSPLHLQPPPPG-----RRAPELQAFF 1246

  Fly  1508 QQQSLAMPP------PPPPPYNVPPLGSIYSHHQGTAG-SRHLNHMHGKT 1550
            ..|:.:..|      |.|||  .||||........||| ..|:..:..||
Zfish  1247 PPQTPSPSPLGPRKHPEPPP--EPPLGPPVPPRLSTAGPPAHIPKLPPKT 1294

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999	19/108 (18%)
		RasGEFN	279..400	CDD:214571	27/128 (21%)
		PDZ_RapGEF2_RapGEF6-like	404..496	CDD:467237	22/107 (21%)
		RA_PDZ-GEF1	759..841	CDD:340483	18/147 (12%)
		RasGEF	871..1050	CDD:459872	47/189 (25%)
sos1	NP_001428581.1	None