DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment PDZ-GEF and RAPGEF6

DIOPT Version :9

Sequence 1:	NP_609012.2	Gene:	PDZ-GEF / 33881	FlyBaseID:	FBgn0265778	Length:	1573	Species:	Drosophila melanogaster
Sequence 2:	NP_001157858.1	Gene:	RAPGEF6 / 51735	HGNCID:	20655	Length:	1609	Species:	Homo sapiens

Alignment Length:	1621	Identity:	554/1621 - (34%)
Similarity:	790/1621 - (48%)	Gaps:	348/1621 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly    42 SHSSDTSSAYSGSDTMASNY-ASSLEAEEIDLSGLVESVVDS----DEEDLAESMDSLTVRDAVR 101
            |.|..:.::.|||.:::..| |:..|..::||:.|.|..|||    :|::..:..|.|..||.||
Human   189 SQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVR 253

  Fly   102 DCLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMVFAVVDKAGTVVMSDGEELDSW 166
            :||||:||:::::|:|.||||...|.||.|:|::|||.|||||:|.||::||.:::.||:|||||
Human   254 ECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSW 318

  Fly   167 SVLINGAVEIEHANGSREELQMGDSFGILPTMDKLYHRGVMRTKCDDCQFVCITQTDYYRIQHQG 231
            .|::||.|||.|.:|..|.|.||:||||.||:||.|..|::|||.||||||||.|.||:||.:..
Human   319 YVILNGTVEISHPDGKVENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHV 383

  Fly   232 EENTRRHEDENGFVVMVTELRSIGGAGTDSAGSGGSATGASASLNMKRGHVVIRGTPERLLQQLV 296
            |:||.:.|:| |.:|||.|.|.:..:||                  ::||:||:.|||||:..|:
Human   384 EKNTHKVEEE-GEIVMVHEHRELDRSGT------------------RKGHIVIKATPERLIMHLI 429

  Fly   297 EENSMTDPTYVEDFLLTHRIFIQNPQEVTSKLLHWFDLEQVDAHKTQELRDRVTRVVLLWVNNHF 361
            ||:|:.||||:||||||:|.|:::|.:|..|||.||        |...|||:|||:|||||||||
Human   430 EEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWF--------KIDSLRDKVTRIVLLWVNNHF 486

  Fly   362 TDFEADYEMMEFLEVFEALLERKKLLSQLRLLHIACAAKARMRSCTLTRSSRDEPLNFRIVGGYE 426
            .|||.|..|..|||.||..||..|:...||||:|||||||:.|...|.::||:.||.|.:.||.|
Human   487 NDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSE 551

  Fly   427 LRGVAIATGNAAVGIYISHVEPGSKAQDVGLKRGDQIHEVNGQSLDHVTSKRALEILTGTTHLSI 491
                      ...||::..|||||||.|.||||||||.|||||:.:::|..:|:|||...|||::
Human   552 ----------KGFGIFVEGVEPGSKAADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLAL 606

  Fly   492 SVKSNLLGFKEIMQALEHGGGTAGSGSISAGSGSFKSVRSPRRICANDIAKLHGRSDSTTDELSS 556
            :||:|:..|||::...|..         .:|......: :.::...:.|..:.|..:.|:.|..|
Human   607 TVKTNIFVFKELLFRTEQE---------KSGVPHIPKI-AEKKSNRHSIQHVPGDIEQTSQEKGS 661

  Fly   557 --VSASNRAHMVRLSSVDMLLDQPDCAPPQTPPVSGSGNMASNFMQQLLQSVNNSSAKKSGGNSN 619
              |.|:.                          |||..|.....:.:...|:........||.|.
Human   662 KKVKANT--------------------------VSGGRNKIRKILDKTRFSILPPKLFSDGGLSQ 700

  Fly   620 SDQQDTKGGFMTLAPKRRLQKALAKMNLLNKQNHGSSLNDSSDTLLNDPKSKLSAVSSCSSSTQS 684
            |......|       .|..:.:||.|.:      ..:|:.||..||....|.|..
Human   701 SQDDSIVG-------TRHCRHSLAIMPI------PGTLSSSSPDLLQPTTSMLDF---------- 742

  Fly   685 SINGCTVSGGGRLYQSQSNPDLTSLNYDGGSDAGGNGGGRLQVNYLNAHIHRPSAASTLTTNSTQ 749
                             |||.
Human   743 -----------------SNPS-------------------------------------------- 746

  Fly   750 SHLLPDYPDHVLKVYKADQTCKYVLIYKETTAHEVVMLTLQEFGIHDPSSNFSLCEVSVGDGGMV 814
                 |.||.|::|:|.||...|::|.|:|||.|||...:.|||:...|..:|||||||...|::
Human   747 -----DIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEVSVTPEGVI 806

  Fly   815 KQRRLPDQLQNLAERISFAARYYLKLNDSTEPLVPDELALELVRESNVHFLHLNAYELAIQLTLQ 879
            ||||||||...||:||....|||||.|..||.|..||.|.|||:||.:..|.|:..|:|.||:::
Human   807 KQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMR 871

  Fly   880 DFANFRQIESTEYVDELFELRSRYGVPMLSKFAELVNREMFWVVSEICAEHNIVRRMKIVKQFIK 944
            ||..||.||.|||:|:||:|.|:.|...|.:|.::||:|.|||.|||..|.|.::||||:|.|||
Human   872 DFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWVASEILTEANQLKRMKIIKHFIK 936

  Fly   945 IARHCKECRNFNSMFAIVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDPSRNMSKYRQLVS 1009
            ||.||:||:||||||||:|||...:|:|||.|||||||||::...||||:.||||||:|||.::|
Human   937 IALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDIFDPSRNMAKYRNILS 1001

  Fly  1010 AELLAQHPIIPFYPIVKKDLTFIHLGNDTRVDGLINFEKLRMLAKEVRLLTHMCSSPYDLLSILE 1074
            ::.: |.||||.:|:||||:||:|.|||::||||:|||||||::||:|.:..|.|:..|...:..
Human  1002 SQSM-QPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFR 1065

  Fly  1075 LKGQSPSNALFSLNQMSASQSNAAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKA 1139
            .:    .....||..:|...:|:   .::....|  ..|:|.:.::..|.||::|:|||.|:||.
Human  1066 QR----KKRWRSLGSLSQGSTNS---NMLDVQGG--AHKKRARRSSLLNAKKLYEDAQMARKVKQ 1121

  Fly  1140 YLNSLKILSDEDLLHKFSLECEPAHGSTYSGSISHGNTSHRSGGGGSISGGAGGSSG-------- 1196
            ||:||.:.:||:.....||:.|||:     |:::...:..||.....:|.....|:|
Human  1122 YLSSLDVETDEEKFQMMSLQWEPAY-----GTLTKNLSEKRSAKSSEMSPVPMRSAGQTTKAHLH 1181

  Fly  1197 ---------------------GGGGGSSSLNAG---------DQLSIYSHTSSSSAPNSSLSLRK 1231
                                 .|.....:||..         :::|...||..:.:..|  ||..
Human  1182 QPHRVSQVLQVPAVNLHPIRKKGQTKDPALNTSLPQKVLGTTEEISGKKHTEDTISVAS--SLHS 1244

  Fly  1232 RHPSSP--------TLSTTSSTSSTSDHQRRQMHNNGPKFGTASPQAVKKMLSLSESSKIRPHQP 1288
            ..|:||        ||..::.:.:.||....::.:   :....|..:|..|.:..:..:......
Human  1245 SPPASPQGSPHKGYTLIPSAKSDNLSDSSHSEISS---RSSIVSNCSVDSMSAALQDERCSSQAL 1306

  Fly  1289 FVPRHGSTMAGVIPPLHHM-----HAAHGFSTPSPGGVVTSPA-------TSAVAN--------- 1332
            .||.  ||  |.:....|.     |:.||     ||..:..|:       :|:|:|
Human  1307 AVPE--ST--GALEKTEHASGIGDHSQHG-----PGWTLLKPSLIKCLAVSSSVSNEEISQEHII 1362

  Fly  1333 -----------VQCTPS--------PSPCSHRRLASGGNIIPSRAIHERSHSDTP-APPPPLPSV 1377
                       ..|:.|        |:|.|...|.|          :..:|.|.| |...|..|.
Human  1363 IEAADSGRGSWTSCSSSSHDNFQSLPNPKSWDFLNS----------YRHTHLDDPIAEVEPTDSE 1417

  Fly  1378 DLSLESSSVTT---FRDLPLRKSVTSGSISS--CDSGYVHQQQHYHLQYQQQQQQNSSQHEPSPP 1437
            ..|...|...|   .:....|||.||.|..|  .:..|...:|........:..:.....:.:.|
Human  1418 PYSCSKSCSRTCGQCKGSLERKSWTSSSSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKDATDP 1482

  Fly  1438 VYTAADCRLLQQISNNAVTRNLNSPCQSTNTPPSTPTPP-------------PNQPTATIQLSAP 1489
            ||..    :........:...:.||.:........||||             |:     ..|..|
Human  1483 VYKT----VTSSTEKGLIVYCVTSPKKDDRYREPPPTPPGYLGISLADLKEGPH-----THLKPP 1538

  Fly  1490 PTAAAYMHARSQHQQLQQQQQSLAMPP----------------PPPPPYNV----PPLGSI 1530
            ..:.|...::..|..|.:      :||                ..|..:|:    |.||.:
Human  1539 DYSVAVQRSKMMHNSLSR------LPPASLSSNLVACVPSKIVTQPQRHNLQPFHPKLGDV 1593

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999	65/108 (60%)
		RasGEFN	279..400	CDD:214571	69/120 (58%)
		PDZ_signaling	404..494	CDD:238492	41/89 (46%)
		DegQ	<422..494	CDD:223343	34/71 (48%)
		PDZ_GEF_RA	757..841	CDD:176380	45/83 (54%)
		RasGEF	871..1050	CDD:279011	108/178 (61%)
		Rhomboid_SP	1252..>1357	CDD:289371	25/144 (17%)
RAPGEF6	NP_001157858.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..22
		CAP_ED	37..>82	CDD:294041
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	179..250		18/60 (30%)
		Crp	274..>448	CDD:223736	100/192 (52%)
		CAP_ED	280..380	CDD:237999	61/99 (62%)
		RasGEFN	412..525	CDD:214571	69/120 (58%)
		PDZ_signaling	529..609	CDD:238492	41/89 (46%)
		UBQ	749..833	CDD:294102	45/83 (54%)
		RasGEF	857..1081	CDD:238087	122/228 (54%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1200..1282		16/86 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1310..1332		7/30 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1463..1486		3/22 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1579..1609		4/15 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	239	1.000	Domainoid score	I2286
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	882	1.000	Inparanoid score	I402
Isobase	1	0.950	-	1	Normalized mean entropy	S4867
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D21656at33208
OrthoFinder	1	1.000	-	-		FOG0002053
OrthoInspector	1	1.000	-	-		otm41882
orthoMCL	1	0.900	-	-		OOG6_104221
Panther	1	1.100	-	-	LDO	PTHR23113
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1353
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	12	11.870