DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment PDZ-GEF and Rapgef5

DIOPT Version :9

Sequence 1:	NP_609012.2	Gene:	PDZ-GEF / 33881	FlyBaseID:	FBgn0265778	Length:	1573	Species:	Drosophila melanogaster
Sequence 2:	NP_001382072.1	Gene:	Rapgef5 / 362799	RGDID:	1561668	Length:	880	Species:	Rattus norvegicus

Alignment Length:	1106	Identity:	222/1106 - (20%)
Similarity:	359/1106 - (32%)	Gaps:	463/1106 - (41%)

- Green bases have known domain annotations that are detailed below.

  Fly    87 LAESMDSLTVRDAVRDCLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMVFAVVDK 151
            |.|.:..:..|..:.| |....||.|||..:.:|         ..:|.||:|.|.:|:.......
  Rat   206 LLELVHLIPARAGICD-LSHQKAEDSEESSDEIL---------ARLTSAVQRELAAVIALKARKS 260

  Fly   152 AGTVVMSDGEELDSWSVLINGAVEIEHANGSREELQMG--------DSFGILPTMDKLYHRGVMR 208
            |   :..|.|..|.       .|.:..||....:.|.|        |..|.:..:.||..
  Rat   261 A---IEQDDENTDK-------HVTVTEANDDAPDPQAGVMCKLQERDDIGRIELVHKLAR----- 310

  Fly   209 TKCDDCQFVCITQTDYYRIQHQGEENTR-RHEDENGFVVMVTELRSIGGAGTDSAGSGGSATGAS 272
               ::|||:...:.:..:.:.|.:|.|. :.:|:...|:::.::.|.|.|.|         ||::
  Rat   311 ---ENCQFLQTDKKEPEKSEQQDDEVTMVQVKDQGQSVLVLKKVASCGPAPT---------TGSA 363

  Fly   273 ASLNMKRGHVVIRGTPERLLQQLVEENSMTD------PTYVEDFLLTHRIFIQNPQEVTSKLLHW 331
            .|   ...:||:.||||::|:.|:.:..:.:      .|.::|||||:.:|:.......:.|.|:
  Rat   364 ES---DARYVVVSGTPEKILEHLLNDLHLAEVQHKETETLLDDFLLTYTVFMTTDDLCQALLRHY 425

  Fly   332 FDLEQVDAHKTQEL---RDRVTRVVLLWVNNHFTDFEADYEMMEFLEVFEALLERKKLLSQLRLL 393
            ...:.....:..::   :.:|..:|..|::.:......|.....||:
  Rat   426 SAKKYQGEEENSDVPCRKRKVLHLVSQWISLYKDWLHEDEHSKMFLK------------------ 472

  Fly   394 HIACAAKARMRSCTLTRSSRDEPLNFRIVGGYELRGVAIATGNAAVGIYISHVEPGSKAQDVGLK 458
                         |:.|:..|:...:.|:                      ..|.....:.:|:.
  Rat   473 -------------TIYRNVLDDVYEYPIL----------------------EKELKEFQKILGMH 502

  Fly   459 RGDQIHEVNGQSLDHVTSKRALEILTGTTHLSISVKSNLLGFKEIMQALEHGGGTAGSGSI---- 519
            |...:.|.:.|.     ..:||      .| ..|:|.|.         |:|.|..|.:..|
  Rat   503 RRHTVDEYSPQK-----KNKAL------FH-QFSLKENW---------LQHRGTVAETEEIFCHV 546

  Fly   520 SAGSGSFKSVRSPRRICANDIAKLHGRSDSTTDELSSVSASNRAHMVRLSSVDMLLDQPDCAPPQ 584
            .....|:.||::              :..||..|:..|.|
  Rat   547 YVTEHSYISVKA--------------KVSSTAQEILKVVA------------------------- 572

  Fly   585 TPPVSGSGNMASNFMQQLLQSVNNSSAKKSGGNSNSDQQDTKGGFMTLAPKRRLQKALAKMNLL- 648
                                                               .:||:|...:.|:
  Rat   573 ---------------------------------------------------EKLQRAEEDLALVA 586

  Fly   649 ---NKQNHGSSLNDSSDTLLNDPKSKLSAVSSCSSSTQSSINGCTVSGGGRLYQSQSNPDLTSLN 710
               :.:.|....||                .:.|.|.::|         ||:|
  Rat   587 IMFSGEKHEFQPND----------------LAISKSLEAS---------GRIY------------ 614

  Fly   711 YDGGSDAGGNGGGRLQVNYLNAHIHRPSAASTLTTNSTQSHLLPDYPDHVLKVYKADQTCKYVLI 775
                                                                ||:.|
  Rat   615 ----------------------------------------------------VYRKD-------- 619

  Fly   776 YKETTAHEVVMLTLQEFGIHDPSSNFSLCEVSVGDGGMVKQRRLPDQLQNLAERISFAARYYLKL 840
                                                       |.|.|...||
  Rat   620 -------------------------------------------LADTLNPFAE------------ 629

  Fly   841 NDSTEPLVPDELALELVRESNVHFLHLNAYELAIQLTLQDFANFRQIESTEYVDELFELRSRYG- 904
            |:.::             :.::..|.:|.::||::|...|::.|..|...|.:...|   ||.|
  Rat   630 NEESQ-------------QRSMRILGMNTWDLALELMSFDWSLFNSIHEQELIYFTF---SRQGS 678

  Fly   905 ---VPMLSKFAELVNREMFWVVSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNSMFAIVSGLG 966
               ...||...:..|....||.:||.....:.:|:::||:|||||.|||..:|.||.||||.||.
  Rat   679 GEHTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKKFIKIAAHCKAQQNLNSFFAIVMGLN 743

  Fly   967 HGAVSRLRQTWEKLPSKYQRLFNDLQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYPIVKKDLTF 1031
            ..:||||.|||||:|.|:::||::|:.|.|||.|...||   .|....:.|.|||.|::.||:||
  Rat   744 TASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYR---DAFKKMKPPKIPFMPLLLKDVTF 805

  Fly  1032 IHLGNDTRVDGLINFEKLRMLAKEVRLLTHMCSSPYDLLSILELKGQSPSNALFSLNQMSASQSN 1096
            ||.||.|.:|.|:|||||.|:|..||.|.| |.:                      ||..:..|
  Rat   806 IHEGNKTFLDNLVNFEKLHMIADTVRTLRH-CRT----------------------NQFGSDMS- 846

  Fly  1097 AAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDEDLLHKFSLECE 1161
                                       ||:..|       :|:|:|.|.::..:..|.:.|...|
  Rat   847 ---------------------------PKEQQE-------LKSYVNHLYVIDSQQALFELSHRLE 877

  Fly  1162 P 1162
            |
  Rat   878 P 878

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999	25/117 (21%)
		RasGEFN	279..400	CDD:214571	23/129 (18%)
		PDZ_signaling	404..494	CDD:238492	13/89 (15%)
		DegQ	<422..494	CDD:223343	9/71 (13%)
		PDZ_GEF_RA	757..841	CDD:176380	8/83 (10%)
		RasGEF	871..1050	CDD:279011	80/182 (44%)
		Rhomboid_SP	1252..>1357	CDD:289371
Rapgef5	NP_001382072.1	DEP_Epac	98..221	CDD:239884	3/14 (21%)
		RasGEFN	368..499	CDD:214571	28/183 (15%)
		RasGEF	640..879	CDD:214539	104/303 (34%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810