DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment PDZ-GEF and rapgef6

DIOPT Version :9

Sequence 1:	NP_609012.2	Gene:	PDZ-GEF / 33881	FlyBaseID:	FBgn0265778	Length:	1573	Species:	Drosophila melanogaster
Sequence 2:	XP_017208494.2	Gene:	rapgef6 / 335032	ZFINID:	ZDB-GENE-030131-6972	Length:	1546	Species:	Danio rerio

Alignment Length:	1570	Identity:	575/1570 - (36%)
Similarity:	801/1570 - (51%)	Gaps:	315/1570 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    35 HQKCNRGSHSSDTSSAY-SGSDTMASNY-ASSLEAEEIDLSGLVESVVDSDE----EDLAESMDS 93
            ||:.:..:..|..|.|. |||.:::..| |:..|..::|||||.|:.|||:|    ||:..|.|:
Zfish   190 HQQMHMANSQSGCSIASDSGSSSLSDIYQATESELGDVDLSGLPEAPVDSEEEEEDEDMDRSSDA 254

  Fly    94 LTVRDAVRDCLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMVFAVVDKAGTVVMS 158
            |..||.||:|||||..:|:::|:|.||||...|.||.|:|::|||.||.||:|.||:..|||::.
Zfish   255 LLARDLVRECLEKDALDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCRVMMFEVVEGEGTVILQ 319

  Fly   159 DGEELDSWSVLINGAVEIEHANGSREELQMGDSFGILPTMDKLYHRGVMRTKCDDCQFVCITQTD 223
            |.:|||.|.|::||||||.:::|..|.|.||:||||.|::|:....|.:.|:.||||||||.|.|
Zfish   320 DKQELDLWYVILNGAVEISYSDGRSEILCMGNSFGITPSLDRQLMSGAVSTRADDCQFVCIAQQD 384

  Fly   224 YYRIQHQGEENTRRHEDENGFVVMVTELRSIGGAGTDSAGSGGSATGASASLNMKRGHVVIRGTP 288
            |.||.:..|:||.:.|:| |.:|||.|.|.:..:||                  ::||:||:|||
Zfish   385 YCRILNHVEQNTHKVEEE-GEIVMVKEHRELDRSGT------------------RKGHIVIKGTP 430

  Fly   289 ERLLQQLVEENSMTDPTYVEDFLLTHRIFIQNPQEVTSKLLHWFDLEQVDAHKTQELRDRVTRVV 353
            |||:..||||.|:.||||:|||||.:|.|:.:|.||.:|||.||   :|||     |||.|||:|
Zfish   431 ERLVLHLVEEPSVVDPTYIEDFLLMYRTFLSSPMEVGNKLLEWF---RVDA-----LRDTVTRIV 487

  Fly   354 LLWVNNHFTDFEADYEMMEFLEVFEALLERKKLLSQLRLLHIACAAKARMRSCTLTRSSRDEPLN 418
            ||||||||.|||.|..|.:|||.||.||:..|:...||||:|||||||:.|..||.:|.|:.||.
Zfish   488 LLWVNNHFNDFEGDPVMTQFLEDFEKLLDASKMKGHLRLLNIACAAKAKCRQVTLQKSCRESPLM 552

  Fly   419 FRIVGGYELRGVAIATGNAAVGIYISHVEPGSKAQDVGLKRGDQIHEVNGQSLDHVTSKRALEIL 483
            |.:.||.| ||         .||:|..|||.|:|.:.|||||||:.|:|||:.::::..:|::||
Zfish   553 FNLQGGSE-RG---------FGIFIESVEPNSRAAEAGLKRGDQVLEINGQNFENISYTKAMDIL 607

  Fly   484 TGTTHLSISVKSNLLGFKEIMQALEHGGGTAGSGSISAGSGSFKSVRSPRRICANDIAKLHGRSD 548
            ...||||::||:|:..|||:...:.|.....|.                      .|.|:..|.
Zfish   608 KNNTHLSLTVKTNIFVFKELQSRMRHEKKNGGP----------------------HIPKIQERK- 649

  Fly   549 STTDELSSVSASNRAHMVRLSSVDMLLDQPDCAPPQTPPVSGSGNMASNFMQQLLQSVNNSSAKK 613
                       |||..:                 |..|     |:|..        ..::.|.||
Zfish   650 -----------SNRFSI-----------------PDLP-----GDMEF--------PTDSKSHKK 673

  Fly   614 SGGNSNSDQQDTKGGFMTLAPKRRLQKALAKMNLLNKQNHGSSLNDSSDTLLNDPKSKLSAVSSC 678
            ...|:.|      ||      :.:::|.|.|...               ::|  |....|....|
Zfish   674 MKANTVS------GG------RNKIRKMLEKTRF---------------SIL--PPKPFSDSGVC 709

  Fly   679 SSSTQSSI--NGCTVSGG-----GRLYQSQSNPDLTSLNYDGGSDAGGNGGGRLQVNYLNAHIHR 736
            .|...|.:  ..|..|..     |.|  |.|:|||                  ||
Zfish   710 QSQDDSIVGTKQCRHSVAIMPIPGSL--SSSSPDL------------------LQ---------- 744

  Fly   737 PSAASTLTTNSTQSHLLPDYPDHVLKVYKADQTCKYVLIYKETTAHEVVMLTLQEFGIHDPSSNF 801
              .|:|:...|..|    |.||.|::|:||||...|::|.|:|||.:||...:.|||:...:..:
Zfish   745 --PAATVLDFSNPS----DIPDQVIRVFKADQQSAYIIISKDTTAKDVVSHVVSEFGLIAAAETY 803

  Fly   802 SLCEVSVGDGGMVKQRRLPDQLQNLAERISFAARYYLKLNDSTEPLVPDELALELVRESNVHFLH 866
            |||||||...|::|||||||||..||:||....|||||.|..||.|..||.|.:|:|||.:..|.
Zfish   804 SLCEVSVSPEGVIKQRRLPDQLSKLADRIQLNGRYYLKNNMETETLCSDEDAQDLLRESQISLLQ 868

  Fly   867 LNAYELAIQLTLQDFANFRQIESTEYVDELFEL--RSRYGVPMLSKFAELVNREMFWVVSEICAE 929
            |:..|:|.||:::||:.||.||||||||:||:|  ....|...|.:|.|::|:|.|||.:||..|
Zfish   869 LSTVEVAAQLSMRDFSLFRNIESTEYVDDLFKLDPGGGGGAGHLKQFEEIINQETFWVSTEILRE 933

  Fly   930 HNIVRRMKIVKQFIKIARHCKECRNFNSMFAIVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDL 994
            .|.::|||.:|.|||||.||:||:||||||||:|||....|:|||.|||||||||::||.||||:
Zfish   934 PNAIKRMKTIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRSTWEKLPSKYEKLFRDLQDI 998

  Fly   995 MDPSRNMSKYRQLVSAELLAQHPIIPFYPIVKKDLTFIHLGNDTRVDGLINFEKLRMLAKEVRLL 1059
            .||||||:|||.::|::.: |.||||.:|:|||||||:|.|||:.||||:|||||||:|||:|.:
Zfish   999 FDPSRNMAKYRNILSSQSV-QPPIIPLFPVVKKDLTFLHEGNDSSVDGLVNFEKLRMIAKEIRHV 1062

  Fly  1060 THMCSSPYD-LLSILELKGQSPSNALFSLNQMSASQSNAAAGTVIAANAGQATIKRRKKSTAAPN 1123
            ..|.|:..| .|...:.|.:..     ||..:|...:|:....|      |...|:|.:.::..|
Zfish  1063 VRMTSANMDPALMFRQRKKRWK-----SLGSLSQGSTNSNLLDV------QGNHKKRVRRSSLLN 1116

  Fly  1124 PKKMFEEAQMVRRVKAYLNSLKILSDEDLLHKFSLECEPAHGSTYSGSISHGNTSHRSGGGGSIS 1188
            .||::|:|||.|:||.||..|::.:||:.....||:||.|: ||.|.::|...::.......|:.
Zfish  1117 AKKLYEDAQMARKVKQYLAHLEVETDEEKFQIMSLQCEAAY-STLSKNLSERRSTKSDMSPVSLR 1180

  Fly  1189 GGAGGSSGGGGGGSSSLNAGDQL--------------SIYSHTSSSSAPNSSLSLRK-------R 1232
            .|.        ..|.:.|...|:              ||....:::.:|||..:|:|       .
Zfish  1181 SGL--------SSSRTQNRVSQVLQVPPVSLYPLRKKSIAKDLAATFSPNSPQTLKKSAGAAEDS 1237

  Fly  1233 HPSSPTLSTTSSTSSTSDHQRRQMHNNGPKFGTASPQAVKKMLSLSESSKIRPHQPFVPRHGSTM 1297
            ..:..:|||.||..|:.               |.|||...:.:|:|.|   |.:....|..|...
Zfish  1238 RRTEESLSTVSSLHSSP---------------TVSPQGSPRKVSVSSS---RQNGSSAPAPGGRT 1284

  Fly  1298 AGV---IPPLHHMHAAHGFSTPSPGGVVT-----SPATSAVANVQC-----TPSPSPCSHRRLAS 1349
            .|:   :.|......:......|...:|:     |..|:|.|:|..     |.:|:.||......
Zfish  1285 YGIGYALMPAGRSDLSDSSEISSRSSIVSNCSVDSGLTAAPASVPAETHTLTHTPADCSWSSSVR 1349

  Fly  1350 GGNIIPSRAIHERSHSDTPAPPPPLPSVDLSLESSSVTTFRDLPLRKSVTSGSISSCDSGYVHQQ 1414
            ...:..|..  ..|..:..||           |.|||:...|.. |.|.||.|.:|.||
Zfish  1350 DSTVALSTV--SNSAEELTAP-----------EHSSVSEIADSG-RASWTSCSSNSQDS------ 1394

  Fly  1415 QHYHLQ-YQQQQQQNSSQHEPSPPVYTAADCRLLQQ---------ISNNA-----------VTRN 1458
                || .|:..:..|.:|.|........|..||::         :.:|:           :|.:
Zfish  1395 ----LQTVQRAPEPLSLRHTPMGGPIAEVDPALLEERDSEDGYDPVKHNSTDPADGSDYKTITSS 1455

  Fly  1459 ---------LNSPCQSTNTPPSTPTPPPNQPTATIQLSAPPTAAAYMHARSQHQQLQQQ---QQS 1511
                     :.|||:........||||..|   .:.|.|.....|......:::...|.   ::|
Zfish  1456 TEKGLIVYCVTSPCKDERFRAPPPTPPGYQ---GLTLGAAQAQDAQKLKPPEYKVALQSCRLRRS 1517

  Fly  1512 LAMPPPPPPP 1521
            ||.|.|...|
Zfish  1518 LAEPQPELEP 1527

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999	58/108 (54%)
		RasGEFN	279..400	CDD:214571	73/120 (61%)
		PDZ_signaling	404..494	CDD:238492	39/89 (44%)
		DegQ	<422..494	CDD:223343	31/71 (44%)
		PDZ_GEF_RA	757..841	CDD:176380	45/83 (54%)
		RasGEF	871..1050	CDD:279011	110/180 (61%)
		Rhomboid_SP	1252..>1357	CDD:289371	23/117 (20%)
rapgef6	XP_017208494.2	CAP_ED	49..154	CDD:320730
		cNMP	290..405	CDD:197516	60/115 (52%)
		RasGEFN	421..534	CDD:214571	73/120 (61%)
		PDZ_signaling	538..618	CDD:238492	39/89 (44%)
		UBQ	759..843	CDD:320785	45/83 (54%)
		RasGEF	866..1102	CDD:214539	129/247 (52%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	236	1.000	Domainoid score	I2273
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	852	1.000	Inparanoid score	I416
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D21656at33208
OrthoFinder	1	1.000	-	-		FOG0002053
OrthoInspector	1	1.000	-	-		otm25544
orthoMCL	1	0.900	-	-		OOG6_104221
Panther	1	1.100	-	-	LDO	PTHR23113
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1353
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.970