Protein Alignment PDZ-GEF and Ralgdsl2

DIOPT Version :10

• Sequence 1: NP_609012.2
• Gene: PDZ-GEF / 33881
• FlyBaseID: FBgn0265778
• Length: 1573
• Species: Drosophila melanogaster
• Sequence 2: NP_997712.1
• Gene: Ralgdsl2 / 294283
• RGDID: 1302985
• Length: 778
• Species: Rattus norvegicus
• Alignment Length: 698
• Identity: 161/698 - (23%)
• Similarity: 245/698 - (35%)
• Gaps: 203/698 - (29%)

  Fly   714 GSDAGGN--GGGRLQVNYLNAHIHRPSAAS-----------TLTTNSTQSHLL-PDYP------- 757
            |.|.||.  |||:.:..      .....||           |.|..|.|...| |..|
  Rat    31 GGDPGGRAVGGGQEEEE------EEEEEASVSIWDEEEDGATFTVTSRQYRPLDPSAPLPPPRSS 89

  Fly   758 ------------DHVL--KVYKADQTCKYVLIYKETTAHEVVMLTLQEFGI----------HDPS 798
                        .|:|  :...||.|....|:    ..|.....|...||:          |.|.
  Rat    90 RRLRAGTLEALVRHLLDARTSGADMTFTPALL----ATHRAFTSTPALFGLVADRLEALESHPPG 150

  Fly   799 SNFSLCEVSVG-----------DGGMVKQRRLPDQLQNLAERISFAAR---------YYLKLNDS 843
            .......|::.           |.|...:.:| |:|::...|..:|||         ....|...
  Rat   151 ELERTTGVAISVLSTWLASHPEDFGSEVKGQL-DRLESFLLRTGYAAREGVVGGSADLIRNLRAR 214

  Fly   844 TEPLVPD---ELALE----------LVRESNVHFLHLNAYELAIQLTLQDFANFRQIESTEYVDE 895
            .:|..||   .|||.          ||      ||   |..||.||||.|...|..:..::.:..
  Rat   215 VDPRAPDLPKPLALPDDSPADPTDVLV------FL---ADHLAEQLTLLDAELFLSLIPSQCLGG 270

  Fly   896 LFELRSRYG----VPMLSKFAELVNREMFWVVSEICA--------EHNIVR------RMKIVKQF 942
            |:..|.|.|    .|.:.......|:....|||.:..        ....||      |.::::::
  Rat   271 LWGHRDRPGHSHLCPSVRATVTQFNKVAGAVVSSVLGATPTGEGPREVTVRPLRPPQRARLLEKW 335

  Fly   943 IKIARHCKECRNFNSMFAIVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDPSRNMSKYRQL 1007
            |::|..|:..|||:|::|:||.|....:.|||..|.:......|:|:.|..:.....|.|:.|:|
  Rat   336 IRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRIFSSLCQIFSEEDNYSQSREL 400

  Fly  1008 VSAELLAQHP-----------------IIPFYPIVKKDLTFIHLGN-DTRVDGLINFEKLRMLAK 1054
            :..|:..|.|                 ::|:.....|||..:...: |...:|.|||:|.|   |
  Rat   401 LMQEVKLQPPVEPHSKKAPRSGSRGGGVVPYLGTFLKDLVMLDAASKDELENGYINFDKRR---K 462

  Fly  1055 EVRLLTHM------CSSPYDLLSILELKGQSPSNALFSLNQMSASQSNAAAGTVIAANAGQATIK 1113
            |..:|:.:      |.. |||....:::     ..|..|..::.:||:..:..|..:....:...
  Rat   463 EFAVLSELLRLQNECRG-YDLRPNPDIQ-----QWLQGLQPLTEAQSHRVSCEVEPSGTIDSPAA 521

  Fly  1114 RRKK-------------STAAPNPKKMFEE------------AQMVRRVKAYL-----NSL-KIL 1147
            |..:             |...|.|...::.            |.::.|:..::     :|| ..|
  Rat   522 RTLRPALVITQWTEVLGSVGGPTPLVSWDRPTVGGDEVPGTPAPLLTRLAQHMKWPSVSSLDSAL 586

  Fly  1148 SDEDLLHKFSLECEPAHGSTYSGSISHGNTSHRSGGGGS-ISGGAG----GSSGGGGG----GSS 1203
            .....||.   ..:|.|.|..:.|........||...|| :|||.|    .|:|.|||    |||
  Rat   587 ESSPSLHS---PADPGHLSPPASSPRPSRGHRRSASCGSPLSGGTGEGASRSAGCGGGVSGPGSS 648

  Fly  1204 -------SLNAGDQLSIYSH---TSSSSAPN-SSLSLRKRHPSSPTLS 1240
                   .:..|:..|:|..   ||...||: .|..|:|.:..|...|
  Rat   649 DCRIIRVQMELGEDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVAS 696

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999
		RasGEFN	279..400	CDD:214571
		PDZ_RapGEF2_RapGEF6-like	404..496	CDD:467237
		RA_PDZ-GEF1	759..841	CDD:340483	22/113 (19%)
		RasGEF	871..1050	CDD:459872	55/214 (26%)
Ralgdsl2	NP_997712.1	RasGEFN	90..212	CDD:214571	22/126 (17%)
		RasGEF	239..509	CDD:238087	72/287 (25%)
		RA_RGL2	649..734	CDD:340731	12/48 (25%)