Protein Alignment PDZ-GEF and RGL1

DIOPT Version :10

• Sequence 1: NP_609012.2
• Gene: PDZ-GEF / 33881
• FlyBaseID: FBgn0265778
• Length: 1573
• Species: Drosophila melanogaster
• Sequence 2: NP_055964.3
• Gene: RGL1 / 23179
• HGNCID: 30281
• Length: 803
• Species: Homo sapiens
• Alignment Length: 681
• Identity: 148/681 - (21%)
• Similarity: 247/681 - (36%)
• Gaps: 195/681 - (28%)

  Fly   735 HRPSAASTLTTNSTQSHLLPDYPDHVLKVYKADQTCKYVLIYKET-----TAHEVVMLTLQEFG- 793
            |..|...|....:.::..|....:::|..: .|....|:.|:..|     :..||:.|.|..:|
Human    91 HTVSQYETCKIRTIKAGTLEKLVENLLTAF-GDNDFTYISIFLSTYRGFASTKEVLELLLDRYGN 154

  Fly   794 IHDPSSNFSLCE----VSVGDGGMVKQ---------------------------RRLPDQL---- 823
            :..|:     ||    .|..:..||.:                           ::|.|.|
Human   155 LTSPN-----CEEDGSQSSSESKMVIRNAIASILRAWLDQCAEDFREPPHFPCLQKLLDYLTRMM 214

  Fly   824 ---------QNLAERISFAARYYLKLNDSTEPLVPDELALELVRE------SNVHFLHLNAYELA 873
                     |||.|:       :.|....|:..:|:.::..|..|      .:..|...:...:|
Human   215 PGSDPERRAQNLLEQ-------FQKQEVETDNGLPNTISFSLEEEEELEGGESAEFTCFSEDLVA 272

  Fly   874 IQLTLQDFANFRQIESTEYVDELFELR----SRYGVPMLSKFAELVNREMFWVVSEICA--EHNI 932
            .|||..|...|:::.....:..::..|    :::..|.:.......|.....|||.|..  |...
Human   273 EQLTYMDAQLFKKVVPHHCLGCIWSRRDKKENKHLAPTIRATISQFNTLTKCVVSTILGGKELKT 337

  Fly   933 VRRMKIVKQFIKIARHCKECRNFNSMFAIVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDP 997
            .:|.||::::|.||..|:..:||:|:.||||.|...::.||::||..:|.....:|.:|.|:...
Human   338 QQRAKIIEKWINIAHECRLLKNFSSLRAIVSALQSNSIYRLKKTWAAVPRDRMLMFEELSDIFSD 402

  Fly   998 SRNMSKYRQLVSAELLAQHPIIPFYPI----------------VKKDL-----TFIHLGN----- 1036
            ..|     .|.|.|||.:.....|..:                ::||:     |..:||.
Human   403 HNN-----HLTSRELLMKEGTSKFANLDSSVKENQKRTQRRLQLQKDMGVMQGTVPYLGTFLTDL 462

  Fly  1037 ---DTRVD-----GLINFEKLRM---LAKEVRLLTHMCSSPYDLLSILELKGQSPSNAL---FSL 1087
               ||.:.     |||||||.|.   :..:::||...|:| |.:         :|....   |..
Human   463 TMLDTALQDYIEGGLINFEKRRREFEVIAQIKLLQSACNS-YCM---------TPDQKFIQWFQR 517

  Fly  1088 NQMSASQSNAAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDEDL 1152
            .|:...:.:.|....|.|.|        ..||.:|.|:|     .||:|:     ||..|..:.:
Human   518 QQLLTEEESYALSCEIEAAA--------DASTTSPKPRK-----SMVKRL-----SLLFLGSDMI 564

  Fly  1153 LHKFSLECEPAHGSTYSG---------SISHGNTSHRSGGGGSISGG----------AGGSSGGG 1198
            ......:.:|.  ||.||         |:|...::|.....|||:..          :..||...
Human   565 TSPTPTKEQPK--STASGSSGESMDSVSVSSCESNHSEAEEGSITPMDTPDEPQKKLSESSSSCS 627

  Fly  1199 GGGSSSLNAGDQLSIYSHTSSSSAPNSSLSLRKRHPSSPTLSTTSSTSSTSDHQRRQMH------ 1257
            ...|...|:....|:.:..||..:.|::..:.||     ::|.||.||:.......|.:
Human   628 SIHSMDTNSSGMSSLINPLSSPPSCNNNPKIHKR-----SVSVTSITSTVLPPVYNQQNEDTCII 687

  Fly  1258 ------NNGPKFGTASPQAVKKMLSLSESSK 1282
                  |||..:         |.:.|:...|
Human   688 RISVEDNNGNMY---------KSIMLTSQDK 709

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999
		RasGEFN	279..400	CDD:214571
		PDZ_RapGEF2_RapGEF6-like	404..496	CDD:467237
		RA_PDZ-GEF1	759..841	CDD:340483	24/131 (18%)
		RasGEF	871..1050	CDD:459872	58/218 (27%)
RGL1	NP_055964.3	RasGEFN	100..231	CDD:214571	24/143 (17%)
		RasGEF	263..532	CDD:238087	69/283 (24%)
		RA_RGL	682..768	CDD:340730	6/37 (16%)