DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment PDZ-GEF and Rasgrf1

DIOPT Version :9

Sequence 1:	NP_609012.2	Gene:	PDZ-GEF / 33881	FlyBaseID:	FBgn0265778	Length:	1573	Species:	Drosophila melanogaster
Sequence 2:	NP_035375.1	Gene:	Rasgrf1 / 19417	MGIID:	99694	Length:	1262	Species:	Mus musculus

Alignment Length:	1152	Identity:	207/1152 - (17%)
Similarity:	371/1152 - (32%)	Gaps:	426/1152 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly    38 CNRGSHSSDTSSAYSGSDTMASNYASSLEAEEIDLSGLVESVVDSDEEDLAESMDSLTVRDAVRD 102
            |.|||         .|...:..|             |:: |::|....|..|::|
Mouse   506 CTRGS---------GGKLHLTKN-------------GVI-SLIDCTLLDEPENLD---------- 537

  Fly   103 CLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMVFAVVDKAGTVVMSDGEELDSWS 167
                |.|:.:..::| .|||..|::...::.            |.|:     :|.|..:|..:|:
Mouse   538 ----DEAKGAGPEIE-HLEFKIGVEPKDSLP------------FTVI-----LVASTRQEKAAWT 580

  Fly   168 VLINGAVEIEHANGSREELQMGDSFGILPTMDK----LYHRGVMRTKCDDCQFVCITQTDYYRIQ 228
            ..|...|:....||........:|...:|.|.|    ||        |||.
Mouse   581 SDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLY--------CDDV-------------- 623

  Fly   229 HQGEENTRRHEDENGFVVMVTELRSIGGAGTDSAGSGGSATGASASLNMKRGHVVIRGTPERLLQ 293
                 :.|..:..|...|:.....|:                                  ||||:
Mouse   624 -----DIRFSKTMNSCKVLQIRYASV----------------------------------ERLLE 649

  Fly   294 QLVEENSMTDPTYVEDFLLTHRIFIQNPQEVTSKLLHWF--DLEQVDAHKTQELRDRVTRVVLLW 356
            :|.:...:: ..::..||.::|:| .|...|..||::.:  .:..:.|...:          ||:
Mouse   650 RLTDLRFLS-IDFLNTFLHSYRVF-TNAMVVLDKLINIYRKPMSAIPARSLE----------LLF 702

  Fly   357 VNNHFTDFEADYEMMEFLEVFEALLERKKLLSQLRLLHIACAAKARMRSCTLTRSSRDEPLNFRI 421
            .::|                      ..|||       ...|.|:...|   .:.|...||
Mouse   703 SSSH----------------------NAKLL-------YGDAPKSPRAS---RKFSSPPPL---- 731

  Fly   422 VGGYELRGVAIATGNAAVGIYISHVEPGSKAQDVGLKRGDQIHEVNGQSLDHVTSKRALEILTGT 486
                     ||.|.:.            |:.:.:.|            ::..:|..:|||:
Mouse   732 ---------AIGTSSP------------SRRRKLSL------------NIPIITGGKALEL---- 759

  Fly   487 THLSISVKSNLLGFKEIMQALEHGGGTAGSGSISAGSGSFKSVRSPRRICANDIAKLHGRSDSTT 551
                                          .|:...|.|:.::.||        ....|::...|
Mouse   760 ------------------------------ASLGCSSDSYANIHSP--------ISPFGKTTLDT 786

  Fly   552 DELSSVSASNRAHMVRLSSVDMLLDQPDCAPPQTPPVSGSGNMASNFMQQLLQSVNNSSAKKSGG 616
            .:|...|:        |......:|.|...|.:...:|.|...:|:.:::..:...|
Mouse   787 GKLCMASS--------LPKTPEEIDVPATIPEKPGELSASRKHSSDVLKEESEDDQN-------- 835

  Fly   617 NSNSDQQDTKGGFMTLAPKRRLQKALAKMNLLNKQNHGSSLNDSSDTLLNDPKSKLSAVSSCSSS 681
            :|:.|..:.........||..|.:.:.:....| .|:|..:....| |:::.:|.|||.|:.:.:
Mouse   836 HSDEDNTEVSPVKSPPTPKSFLNRTITEFPFFN-YNNGILMTTCRD-LVDNNRSTLSATSAFAIA 898

  Fly   682 TQSSINGCTVSGGGRLYQSQSNPDLTSLNYDGGSDAGGNGGGRLQVNYLNAHIHRPSAASTLTTN 746
            |                                  ||.|.|..      |..:.|..:.:....:
Mouse   899 T----------------------------------AGANEGPS------NKEVFRRMSLANTGFS 923

  Fly   747 STQSHLLPDY------PDHVLKV-----------YKADQTCKY-VLIYKETTAHEVVMLTLQEFG 793
            |.|.::..::      .:.||.|           :..|.|.|| |:.:.|...|:..:||.:...
Mouse   924 SDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPDLLTQERKA 988

  Fly   794 IHDPSSNFSLCEVSVGDGGMVKQRRLPDQLQNLAERISFAARYYLKLNDSTEPLVPDELALELVR 858
            ..:.....:|.|.:       :|..:.:::..:.|.:            .|||
Mouse   989 AANIIRTLTLEETT-------EQHSMLEEVILMTEGV------------KTEP------------ 1022

  Fly   859 ESNVHFLHLNAYELAIQLTLQDFANFRQIESTEYVDELFELRSRY-GVPMLSKFAELVNREMFWV 922
                 |.:..|.|:|.||||.|...|:.|...|:..:.:....:| ..|.:.|..:..|....::
Mouse  1023 -----FENHPALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKAEKYERTPYIMKTTKHFNHVSNFI 1082

  Fly   923 VSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNSMFAIVSGLGHGAVSRLRQTWEKLPSKYQRL 987
            .|||....:|..|...:::::.:|..|:...|:|::..|.|.:...|:.||::||.|:..:.:.|
Mouse  1083 ASEIIRNEDISARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSL 1147

  Fly   988 FNDLQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYPIVKKDLTFIHLG--NDTRVDGLINFEKLR 1050
            .:.||.|:...   .:::.|..:......|.:|:..:...||.||..|  |.|. |||:||.|:|
Mouse  1148 LDKLQKLVSSD---GRFKNLRESLRNCDPPCVPYLGMYLTDLVFIEEGTPNYTE-DGLVNFSKMR 1208

  Fly  1051 MLAKEVRLLTHMCSSPYDLLSILELKGQSPSNALFSLNQMSASQSNAAAGTVIAANAGQATIKRR 1115
            |::..:|.:.....:.|.:
Mouse  1209 MISHIIREIRQFQQTTYKI---------------------------------------------- 1227

  Fly  1116 KKSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDEDLLHKFSLECEP 1162
                 .|.||           |..||.....:.||:.|::.||..||
Mouse  1228 -----DPQPK-----------VIQYLLDESFMLDEESLYESSLLIEP 1258

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999	19/112 (17%)
		RasGEFN	279..400	CDD:214571	21/122 (17%)
		PDZ_signaling	404..494	CDD:238492	13/89 (15%)
		DegQ	<422..494	CDD:223343	9/71 (13%)
		PDZ_GEF_RA	757..841	CDD:176380	16/95 (17%)
		RasGEF	871..1050	CDD:279011	51/181 (28%)
		Rhomboid_SP	1252..>1357	CDD:289371
Rasgrf1	NP_035375.1	PH-like	19..155	CDD:302622
		PH	24..130	CDD:278594
		RhoGEF	248..429	CDD:214619
		PH-like	435..586	CDD:302622	26/134 (19%)
		PH	482..587	CDD:278594	26/135 (19%)
		RasGEF_N	638..>687	CDD:279012	14/84 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	714..738		9/39 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	800..854		9/61 (15%)
		REM	<927..996	CDD:295342	12/68 (18%)
		RasGEF	1023..1259	CDD:214539	70/302 (23%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2063
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.940