Protein Alignment PDZ-GEF and pxf-1

DIOPT Version :10

• Sequence 1: NP_609012.2
• Gene: PDZ-GEF / 33881
• FlyBaseID: FBgn0265778
• Length: 1573
• Species: Drosophila melanogaster
• Sequence 2: NP_001023389.1
• Gene: pxf-1 / 177894
• WormBaseID: WBGene00004254
• Length: 1470
• Species: Caenorhabditis elegans
• Alignment Length: 1579
• Identity: 518/1579 - (32%)
• Similarity: 754/1579 - (47%)
• Gaps: 419/1579 - (26%)

  Fly    20 GVVVGSSTTINRPELHQKCN----RGSHSSDTSSAYSGSDTMASNYASSLEAEEIDLSGLVESVV 80
            |:.....|.:......:|.|    :..:...|:....|..|.:|   ::.|.|.....|     .
 Worm   237 GLSADEDTLVRVKHRREKSNSVGGQAQNGISTARRLRGRSTASS---TTTEGETASNEG-----A 293

  Fly    81 DSDEEDLAESMDS--------LTVRDAVRDCLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVR 137
            ||||::  .||.|        :.:||:||:||||:|:||:.||:.|||:|.|.:.||..:.::::
 Worm   294 DSDEDE--GSMPSQESSSGGFMDLRDSVRECLEKEPSERNSEDLAVLLDFMQHMSAFAALPMSIK 356

  Fly   138 RALCSVMVFAVVDKAGTVVMSDGEELDSWSVLINGAVEIEHANGSREELQMGDSFGILPTMDKLY 202
            |.||..||||||:.|||||::..|:||||||::||.||:...:|.|.|.::|||||..||.....
 Worm   357 RQLCLKMVFAVVNDAGTVVLAHNEKLDSWSVIVNGCVEVVKPSGERVEYKLGDSFGAEPTPATQI 421

  Fly   203 HRGVMRTKCDDCQFVCITQTDYYRIQHQ-GEENTRRHEDENGFVVMVTELRSIGGAGTDSAGSGG 266
            |.|.|||..|||:||.:...|:..|... |:...:..:...|.||...|.|::|
 Worm   422 HIGEMRTMVDDCEFVLVEHRDFCSIMSTIGDHIEKDRDGLTGEVVSEVERRTVG----------- 475

  Fly   267 SATGASASLNMKRGHVVIRGTPERLLQQLVEE-NSMTDPTYVEDFLLTHRIFIQNPQEVTSKLLH 330
                      ...|.|:|:|.|::|:..||:| :...||.||:|||||:|:||::|..:..||:.
 Worm   476 ----------THCGQVLIKGKPDKLIHHLVDERDHNVDPHYVDDFLLTYRVFIRDPTTIFEKLML 530

  Fly   331 WFDLEQVDAHKTQELRDRVTRVVLLWVNNHFTDFEADYEMMEFLEVFEALLERKKLLSQLRLLHI 395
            ||        .....||:|.|:|||||||||.|||.:.||...||.||..|||..:.|||.||:|
 Worm   531 WF--------ADSIYRDKVARLVLLWVNNHFNDFETNDEMWNLLERFEGALERDGMHSQLSLLNI 587

  Fly   396 ACAAKARMRSCTLTRSSRDEPLNFRIVGGYELRGVAIATGNAAVGIYISHVEPGSKAQDVGLKRG 460
            ||:.||:.|...||| .:|:.:..|:|||.|       :||:   :|::.|.|.:.|...|:||.
 Worm   588 ACSVKAKPRQVILTR-RKDDKMMMRLVGGQE-------SGNS---VYVAEVFPDTSAAREGVKRA 641

  Fly   461 DQIHEVNGQSLDHVTSKRALEILTGTTHLSISVKSNLLGFKEIMQALEHG---GGTAGSGSISAG 522
            |::.|||.||..::::|:|.::|||:..|::.:|:|:||:||.:..:||.   .||:.||
 Worm   642 DEMLEVNQQSAKYLSAKKAEDLLTGSLSLTLMLKNNVLGYKETIGKIEHNKPKNGTSRSG----- 701

  Fly   523 SGSFKSVRSPRRICANDIAKLHGRSDSTTDELSSVSASNRAHMVRLSSVDMLLDQPDCAPPQTPP 587
                                                          :.:.|::      |.....
 Worm   702 ----------------------------------------------AGIPMVI------PVHKTS 714

  Fly   588 VSGSGNMASNFMQQLLQSVNNSSAKKSGGNSNSDQQDTKGGFMTLAPKRRLQKALAKMNLLNKQN 652
            ::|                     |||...|      :|.|.|        :|.   |.:|.
 Worm   715 ITG---------------------KKSSTTS------SKSGMM--------EKL---MTILK--- 738

  Fly   653 HGSSLNDSSDTLLNDPKSKLSAVSSCSSSTQSSINGCTVSGGGRLYQSQSNPDLTSLNYDGGSDA 717
              ||..||.|  ..| ::|:|:..                    |..|:||||:||::...|.
 Worm   739 --SSKEDSMD--FTD-EAKISSAD--------------------LRPSRSNPDITSISQYYGP-- 776

  Fly   718 GGNGGGRLQVNYLNAHIHRPSAASTLTTNSTQSHLLPDYPDHVLKVYKADQTCKYVLIYKETTAH 782
                                              :..:.|:||||:|:.|||.||:.:||||:|.
 Worm   777 ----------------------------------VRSECPEHVLKIYRNDQTFKYLPVYKETSAQ 807

  Fly   783 EVVMLTLQEFGI-HDPSSNFSLCEVSVGDGGMVKQRRLPDQLQNLAERISFAARYYLKLNDSTEP 846
            .||.|.||||.: .:.|..:||||.:|...|::||||||.|::||||||:..:|||||.|..:||
 Worm   808 NVVQLALQEFNMTAEGSPEWSLCECTVTIDGVIKQRRLPPQMENLAERIALNSRYYLKNNSRSEP 872

  Fly   847 LVPDELALELVRESNVHFLHLNAYELAIQLTLQDFANFRQIESTEYVDELFELRSRYGVPMLSKF 911
            |||||||.||::|:....|.|||..:|.|||||||:.|..||.||::|.||:|.|:||.|.|.:|
 Worm   873 LVPDELAPELLKEAQTQLLSLNAQVVAAQLTLQDFSVFSAIEPTEFLDNLFKLDSKYGSPKLEEF 937

  Fly   912 AELVNREMFWVVSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNSMFAIVSGLGHGAVSRLRQT 976
            .:|.||||:||.:|||.|.::.:|.|::|:|||:||:|::.|||||||||:|||...||.||..:
 Worm   938 EQLFNREMWWVATEICTERHVQKRAKLIKKFIKVARYCRDLRNFNSMFAIMSGLDKPAVRRLHSS 1002

  Fly   977 WEKLPSKYQRLFNDLQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYPIVKKDLTFIHLGNDTRVD 1041
            ||::.|||.|:.:::..|:|||||||||||.: ||:..:.|::|.||::||||||.|.||.|..:
 Worm  1003 WERVSSKYIRMLDEIHQLVDPSRNMSKYRQHL-AEVAQEPPVVPIYPVIKKDLTFAHDGNATYSE 1066

  Fly  1042 GLINFEKLRMLAKEVRLLTHMCSSPYDLLSILELKGQSPSNALFSLNQMSASQSNAAAGTVIAAN 1106
            .||||||||::||.:|.:..:.|:||::.|:.|..|....:||..:|...              |
 Worm  1067 KLINFEKLRLIAKSIRGVMKLSSAPYEIASMAERSGGVVMDALLHMNSFE--------------N 1117

  Fly  1107 AGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDEDLLHKFSLECEPAHGSTYSGS 1171
            :..||:::..........||::|:|.|||:||:||..|.::.:|..|...|.:.||
 Worm  1118 SNVATMRKGMSGKQNQPRKKVYEQALMVRKVKSYLEGLHVVDNEMELDSMSYDIEP--------- 1173

  Fly  1172 ISHGNTSHRSGGGGSISGGAGGSSGGGGGGSSSLNAGDQLSIYSHTSSSSAPNSSLSLRKRHPSS 1236
              ...|:||         ||                                |||.:...|...|
 Worm  1174 --QVQTAHR---------GA--------------------------------NSSSTANIRRVPS 1195

  Fly  1237 PTLSTTSSTSSTSDHQ---RRQMHN---------NGPKFGTASPQAVKKMLSLSESSKIRPHQPF 1289
            ||.|:.||.|:.|..|   .|.:.|         .|.|||..|||||:|||||.::||::
 Worm  1196 PTPSSLSSQSAGSADQSSRHRLLFNGTGSISSAGGGSKFGVESPQAVQKMLSLVQNSKVK----- 1255

  Fly  1290 VPRHGSTMAGVIPPLHHMHAAHGFSTPSPGGVVTSPATSAVANVQCTPSPSPCSHRRLASGGNII 1354
                |:      ||                 .:|||:|||.:::|          |.:
 Worm  1256 ----GA------PP-----------------QITSPSTSARSSLQ----------RNM------- 1276

  Fly  1355 PSRAIHERSHSDTPAPPPPLPSVDLSLESSSVTTFRDLPLRKSVTSGSISSCDSGYVHQQQHY-- 1417
             .|....::.|....|      |.|:.|:|:|||:......:...|||....|:  :.....|
 Worm  1277 -PRVTGRQATSSAQGP------VQLNEETSTVTTYYQSDNGRRQRSGSEGRFDN--IPPSTFYLT 1332

  Fly  1418 --HLQYQQQQQQN----SSQHEPSPPVYTAADCRLLQQISNNAVTRNLNSPCQSTNTPPSTPTPP 1476
              .|....:|..:    :..|..||   |:..||.....|         |.|.|.:|..|.
 Worm  1333 SDGLTVSPRQSLSVVIPTHPHGHSP---TSPRCRSRSPAS---------SGCSSFSTIASI---- 1381

  Fly  1477 PNQPTATIQLSAPPTAAAYMHARSQHQQLQQQQQSLAMPPPPPPPYNVPPLGSIYSHHQ---GTA 1538
                .||...:||   :|::....||.|..:                    |.:..|..   .|:
 Worm  1382 ----AATSMAAAP---SAFVSNPYQHHQTVR--------------------GHVIGHRPMPIVTS 1419

  Fly  1539 GSRHL-NHMHGKTTGPQER 1556
            ||..| ||:..:...|:.|
 Worm  1420 GSATLPNHVSPRGLPPKSR 1438

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999	50/109 (46%)
		RasGEFN	279..400	CDD:214571	59/121 (49%)
		PDZ_RapGEF2_RapGEF6-like	404..496	CDD:467237	32/91 (35%)
		RA_PDZ-GEF1	759..841	CDD:340483	47/82 (57%)
		RasGEF	871..1050	CDD:459872	99/178 (56%)
pxf-1	NP_001023389.1	CAP_ED	45..131	CDD:237999
		cNMP	348..458	CDD:197516	49/109 (45%)
		RasGEFN	479..592	CDD:214571	59/120 (49%)
		PDZ_canonical	596..662	CDD:483948	27/76 (36%)
		RA_PDZ-GEF1	784..867	CDD:340483	47/82 (57%)
		RasGEF	890..1118	CDD:238087	117/242 (48%)